68results about How to "The assay method is fast" patented technology

The invention provides an edible oil quality inspection method based on 1H-nuclear magnetic resonance (NMR) fingerprint spectra and multivariate analysis. The edible oil quality inspection method is mainly characterized by comprising steps of developing 12 1H-NMR fingerprint spectra for identifying swill-cooked dirty oil; establishing an oil 1H-NMR fingerprint spectrum database according to the 12 1H-NMR fingerprint spectra; classifying samples in the database by clustering analysis; establishing a discriminant function, substituting 1H-NMR data of an unknown sample into the discriminant function; and judging the quality of the unknown sample according to a calculated result. The edible oil quality inspection method has the advantages of small sample quantity, simple pretreatment method, high sensitivity, low cost and high accuracy.

The invention discloses a method for detecting a heavy metal content of coal gangue filling reclamation reconstruction soil based on a high spectrum technology. The method comprises the steps of performing sampling on site; collecting a sample spectrum curve; performing data processing; constructing a high spectrum estimation model for inverting soil heavy metal content; constructing a partial least squares regression model; and finally utilizing the partial least squares regression model to detect the heavy metal content of the coal gangue filling reclamation reconstruction soil. Compared with a conventional multiple linear regression analysis method, according to the method, the problem of multicollinearity faced by the multiple linear regression analysis method is solved, spectral information can be summarized and extracted, system information and noise are identified more easily, and thus the content of heavy metal elements can be quantitatively inverted accurately. The simple and quick detection method is provided for large-area low-cost investigation and monitoring of heavy metal pollution of mine lot reclamation land soil, technical means are provided for guaranteeing mine lot reclamation land crop safety, and development and application of a high spectrum remote sensing technology are promoted.

The invention relates to a cement clinker component determination method in cement production, in particular to a method for determining calcium sulfoaluminate content in cement clinker, which comprises the following steps of: connecting experimental instruments such as a tubular type high-temperature furnace, a nitrogen cylinder, a tower type drier, an absorption bottle and a glass rotor flowmeter, and measuring the content of C4A3S in cement clinker according to experimental steps. The method provided by the invention has the advantages of rapid, accurate and reliable determination, simple instrument and equipment, low cost and convenient operation, and is suitable for C4A3S content determination of cement clinker in factory production, thus facilitating timely production control.

The invention discloses a method for determining thermal-conduction resistance of a metal foam porous medium. The method comprises the following steps: placing a to-be-determined metal foam porous medium between a hot plate and a cold plate and carrying out vacuumization; energizing and heating the hot plate at an upper part and cooling the cold plate at a lower part via a cooling module; when the whole heat transfer process reaches a stable state and a certain degree of vacuum is reached and maintained unchanged in a test section, acquiring data like temperature, current and voltage in real time by using a data acquisition and processing system; and processing the acquired data by using the data acquisition and processing system so as to eventually obtain thermal-conduction resistance of the metal foam porous medium and providing a test report. The determination method provided by the invention is rapid and simple and has high accuracy.

The invention relates to a method for measuring hydrogen sulfide in mainstream cigarette smoke, in particular to an ion chromatography method for measuring hydrogen sulfide in mainstream cigarette smoke. The invention provides a method for directly measuring hydrogen sulfide in mainstream cigarette smoke, in order to solve the problem that complete capture and derivatization indirect measurement of hydrogen sulfide in mainstream cigarette smoke are susceptible to interference in prior arts. In the inventive method, an alkaline solution containing ascorbic acid, zinc acetate, ethylenediamine and sodium hydroxide is adopted to capture hydrogen sulfide in the gas phase of cigarette smoke, and anion exchange chromatographic separation and silver working electrode-equipped pulsed amperometric detector are adopted to directly measure hydrogen sulfide (sulfur ions) in the mainstream cigarette smoke. Compared with a conventional method, the inventive method has simpler operation, higher safety and higher interference resistance.

The invention discloses a device for sampling asphalt smoke dust, which consists of a closed container, a heating furnace, a temperature controller, a freezer, an absorption device, a water container and a dry air supplying device, wherein an air outlet of the dry air supplying device is connected with an air inlet of the closed container; an air outlet of the closed container is connected with an air inlet of the absorption device; an air outlet of the absorption device is positioned under the liquid level of the water container; the heating furnace is connected with the temperature controller; a temperature sensing part of the temperature controller is connected with the closed container; the heating furnace is used for heating the closed container; asphalt with unit mass is arranged inthe closed container; the absorption device is arranged in the freezer; adsorption substance is arranged in the absorption device; and the freezer is internally provided with cooling substance. The invention also discloses a method for measuring the content of the asphalt smoke dust. The invention has the beneficial technical effects that the sampling device is simple in structure and convenient to operate, the measurement method is simplified and rapid, and the measurement result is high in accuracy.

The invention discloses an immunodetection kit for detecting adulterated cow milk in camel milk and an application thereof, wherein alpha-lactalbumin and alphas1-casein in camel milk are used as characteristic protein of the camel milk, and beta-lactoglobulin and kappa casein in cow milk are used as characteristic protein of the cow milk. After the camel milk characteristic protein is separated and purified, the polyclonal antibody and the monoclonal antibody prepared by the commercialized cow milk characteristic protein and the self-made camel milk characteristic protein can be applied to ELISA detection. The kit mainly adopts an indirect ELISA method to qualitatively or quantitatively detect the content of camel-derived alpha-lactalbumin, alphas1-casein or bovine-derived beta-lactoglobulin and kappa-casein in camel milk, the sample pretreatment process is simple, and after a camel milk sample is repeatedly centrifuged to obtain a milk sample, the content can be directly measured after diluted by diluent, and a large number of samples can be simultaneously detected. The determination method is accurate, rapid and convenient, and has wide practicability and development value in thefield of camel milk adulteration monitoring.

The invention provides a method of measuring residual liquid hydrocarbon content in shale via low-field nuclear magnetic resonance. A shale sample of any shape is used to saturate manganese chloride solution of sufficient concentration; low-field nuclear magnetic resonance is then performed to obtain a nuclear magnetic T2 spectrum in the shale sample; the shale sample is subjected to dewatering prior to dealkylation; a T2 spectrum signal is measured again; amplitude integral difference delta S of the T2 spectral signal is introduced into a calibration relationship (namely, a marking equation)of hydrocarbon content and the signal, built in the method, so as to calculate hydrocarbon volume in shale of unit volume; the result is that the hydrocarbon volume in shale of unit volume is presented by percentage, which is considered as residual liquid hydrocarbon content in shale. The problems are effectively avoided that samples experience light hydrocarbon loss due to crushing pretreatment and that insufficiency of solvent polarity leads to incomplete extraction of heavy hydrocarbons; the method is suitable for precisely measuring residual hydrocarbon content in shale and is an effectiveand quick measuring method.

The invention discloses a method for detecting the content of methylene blue in blood plasma. The method comprises the following steps of: (a) adding carboxymethyl-beta-cyclodextrin saturated solution into blank blood plasma, enabling the concentration of the mixed solution to reach 1-4.5mmol / L and maintaining the constant volume to obtain a blood plasma blank sample; (b) adding quantitative methylene blue solution into the blank blood plasma and adding the carboxymethyl-beta-cyclodextrin saturated solution to obtain a standard blood plasma sample containing the methylene blue with the concentration of Cs; and (c) adding the carboxymethyl-beta-cyclodextrin saturated solution in a blood plasma sample containing the methylene blue to obtain a blood plasma to-be-measured sample containing the methylene blue with the concentration of Cs; detecting the strength or the area of a fluorescence peak of the sample; and then calculating according to a reference substance comparison method to obtain the concentration Cs of the methylene blue in the blood plasma to-be-measured sample. According to the method disclosed by the invention, the lowest detectable limit is 0.012mumol / L and the unexpected effect is obtained.

The invention discloses a method using a single point adsorption method to measure the specific surface area of atmospheric particulates. The single point adsorption method utilizes a methylene blue water solution and comprises the following steps: (1) collecting atmospheric particulates, cutting, and weighing; (2) preparing a diluted original solution of methylene blue and a standard solution, determining the absorbance of standard solution, and drawing a work curve; (3) determining the absorbance of methylene blue solution, and calculating the adsorption amount of methylene blue according to the work curve; (4) calculating the specific surface area of atmospheric particulates. The method is simple, feasible, fast, and convenient, is suitable for measuring the surface area of atmospheric particulates, and has the characteristics of simple technology, low cost, strong operability, and low instrument requirements.

The invention discloses a method for measuring bacitracin and the content of the preparation thereof by the turbidity method, and the method contains the following steps: the preparation of standard solution, the preparation of test solution, the preparation of test organism suspension, the content measurement, etc. The antibiotics-microbial test and multi-dose turbidity method of the invention are adopted for measuring the bacitracin and the content of the preparation thereof, thus the test method is accurate and effective and has low cost, and test results can truly reflect antibacterial activity of the bacitracin and the preparation thereof; the method has stronger practicality and operability in the production and the quality control of the drug and the preparation thereof.

The invention relates to a method for detecting musk xylene in a tobacco additive and belongs to the technical field of detection of tobacco additives. The method comprises the following steps of: weighing 1.0g of tobacco additive and putting into a 50mL conical flask with a plug, adding 10mL of ultrapure water of which electrical resistivity is 18.2 M Omega and dispersing a sample, adding 0.3mL of D15-musk xylene interior label solution with the concentration of 1mu g / mL, and 10mL of cyclohexane, oscillating and extracting at the temperature of 25 DEG C for 10 minutes, centrifuging a mixed solution at rotation speed of 4,000r / min by a centrifugal machine for 10 minutes, extracting an upper-layer organic phase, adding 10mL of chromatographically pure cyclohexane into a water phase of which the upper-layer organic phase is extracted, performing secondary extraction under the same operation conditions, centrifuging, and extracting an upper-layer organic phase; and mixing the organic phases extracted twice, drying by using anhydrous sodium sulfate, and metering the volume to ensure that the volume is 1mL for gas chromatography-mass spectrometer (GC-MC) determination. The method has the advantages of high sensitivity and capacity of effectively determining the content of musk xylene in the tobacco additive, and can determine the content of the musk xylene accurately and quickly, and is suitable for monitoring and self-inspection work of the highly-concerned musk xylene in the tobacco additive.

The invention relates to a method for measuring perfluorinated compounds in textile, clad layers, coatings, liquid and powder samples. The method comprises the measuring steps that firstly, a sample is cut according to a certain dimension in an area method or a sample of a proper amount is weighed in a weight method, an organic solvent is added for ultrasonic extraction, and the mixture is cooled to the room temperature and then filtered to be fed into a machine for analysis; secondly, the quantity of perfluorinated compounds in the extracted solution obtained in the first step is measured through a liquid chromatogram and mass spectrum combined instrument; thirdly, if a liquid chromatogram and mass spectrum selected ion scanning result shows that perfluorinated compounds seem to be contained in the sample, a spectrogram of the sample is compared with that of a standard substance, if the spectrogram of the sample is matched with that of the standard substance, the extracted solution obtained in the first step is measured through a liquid chromatogram tandem mass spectrum combined instrument, characteristic ions of the sample are compared with those of the standard substance to confirm whether perfluorinated compounds are contained in the sample or not, and the content of perfluorinated compounds in the sample is calculated. The measuring method is fast and simple, and perfluorinated compounds in various materials can be accurately measured.

The invention discloses a tobacco strip aqueous extract absorbance determining and alcoholization process determining method. The method comprises the concrete steps that (1) a tobacco strip is sampled in a five-point sampling method, and crushing is carried out at low temperature; (2) certain weight of the sample is weighed and placed in a 50ml Erlenmeyer flask, certain volume of pure water is added, extraction is carried out for 30 min, and filtering is carried out; (3) colorimetric determination is carried out in the wavelength of 355nm by taking the pure water as a blank sample; and (4) the alcoholization process of the aqueous extract is substituted into a formula, Y=-7.7913X2+45.024X+4.5655, to calculate a tobacco strip sensing quality simulated value. The method is simple and rapidin operation and high in stability, the optimal tobacco strip alcoholization period can be determined accurately, and the color darkness of the tobacco strip can be represented quantitatively. The tobacco strip alcoholization period can be mastered rapidly needless of complex operation or smoking for evaluation, and the working efficiency is improved. The method relates to fewer instruments, and the detection cost is lower.

The invention discloses a method for determining titer of a swine flu inactivated vaccine. The method comprises the following steps: immunizing a pig with a swine flue inactivated vaccine, collecting blood 21-28 days after immunization and separating blood serum; removing non-specific components in the serum to obtain a serum to be tested; diluting the swine flue inactivated antigen, and preparing a unit swine flue antigen diluent with a concentration of 4HA; and diluting the serum to be tested by multiple proportions, successively adding the 4HA unit swine flue antigen diluent and 1% red cells to conduct hemagglutination inhibition test, so as to completely inhibit highest dilution of the 4HA unit swine flu antigen serum at HI titer. The method has advantages of greatly shortened measurement time, little operation error, strong controllability, and small inter-batch difference, and also reduces detection errors caused by different levels of experimental animals in an animal challenge protection experiment for testing the titer.

The invention discloses a device for determination of foaming performance of a foaming agent in CO2 in an oil field and a determination method thereof. The device comprises a separating funnel (1), a metering tube composite structure (2), a jacketed measuring cylinder (3), a sealing rubber plug (4), a breather tube (5) and a one-way valve (6); the metering tube composite structure (2) is mounted at the lower end of the separating funnel (1); the sealing rubber plug (4) is plugged into a mouth part of the jacketed measuring cylinder (3); the sealing rubber plug (4) is provided with a metering tube hole (42), a breather tube hole (41) and a one-way valve hole (43) for respectively inserting the metering tube composite structure (2), the breather tube (5) and the one-way valve (6). The determination method comprises the steps: 1) connecting the device, and filling a test solution; 2) filling the device with CO2; and 3) determining the foaming performance. According to the device provided by the invention, the determination method is rapid, efficient and easy to carry out; and compared with the foaming performance in air measured by international standards and national standards, an obtained determined result is more intuitive and accurate.

The invention discloses a determination method for the chromium content in cotton-seed meal. The determination method includes the steps that different kinds of cotton-seed meal samples planted in different regions are prepared, a near-infrared spectrometer is used for collecting spectrums of the cotton-seed meal samples, full cross validation is adopted in a full spectrum range to build a PLS model, and then the PLS model is analyzed by adopting eight preprocessing methods; a processing method corresponding to the maximum prediction correlation coefficient R2 and the minimum root-mean-square error RMSE is used as the optimal preprocessing method, and a near-infrared spectrum correction model is set up through the optimal preprocessing method; the near-infrared spectrum correction model built in the step mentioned above is used for detecting the cotton-seed meal to be detected to obtain the chromium content of the cotton-seed meal. The FOSS5000 near-infrared spectrometer is used for collecting spectrograms of the cotton-seed meal, determination accuracy is high, and the determination method is fast, convenient and efficient and has great significance in cultivating new cotton species and promoting processing and utilization of cotton by-products.

The invention relates to a method for detecting tanshinone components, and an application thereof. The tanshinone components are dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA. The method comprises the following steps: collecting samples to be tested in the extraction and concentration of Salvia miltiorrhiza, collecting the near infrared spectra of the samples to be tested bynear-infrared spectroscopy, and converting the near infrared spectra into the respective content of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA in every sample to be tested according to a selected model. The method has the advantages of fastness in detection of the respective content of the tanshinone components in a compound Salvia miltiorrhiza tablet, simplicity in thepreparation of the samples, and realization of online analysis.

The invention relates to a construction method of a HPLC specific chromatogram of a daphne genkwa standard decoction and a component content determination method of the daphne genkwa standard decoction, and the component content determination method of the daphne genkwa standard decoction comprises the following steps: (1) respectively sucking a reference substance solution and a to-be-determineddaphne genkwa standard decoction sample solution, injecting the solutions into a high performance liquid chromatograph, and determining corresponding peak areas; (2) calculating the content of genkwanin through an external standard method; and (3) calculating the contents of apigenin-7-O-beta-glucuronide, apigenin and hydroxygenkwanin through a quantitative analysis of multiple components by single marker. According to the method, the content of genkwanin is calculated through an external standard method, and then the content of apigenin-7-O-beta-glucuronide, apigenin and hydroxygenkwanin is calculated through a quantitative analysis method, so that the operation steps of content determination of four components can be effectively simplified, the determination time is shortened, the working efficiency is improved, and quantitative analysis of chemical components of the genkwanin standard decoction is realized.

The invention relates to a method for detecting the content of guanidine phosphate in textile and a filling material. The method comprises the following steps: 1, preprocessing a sample: shearing a textile or filling material sample by scissors or crushing the sample by a crusher in order to form a sample with the dimension size not exceeding 2*2*2 mm, uniformly mixing the sample, filling a clean and dry valve bag with the sample, and sealing the valve bag for later use; 2, extracting the sample: weighing a proper amount of the sample, and carrying out ultrasonic extraction by using a methanol-water solution; 3, detecting the concentration of guanidine phosphate in a sample extract solution obtained in step 2 through adopting liquid chromatography-mass spectrometry; and 4, calculating the content of the guanidine phosphate in the sample according to the concentration of the guanidine phosphate in the sample extract solution, obtained in step 3. The detection method is fast and simple, and can realize accurate detection of the guanidine phosphate in textile and the filling material, the lowest quantification concentration is 5 mg / kg, and a problem that no methods for detecting the content of a fire retardant guanidine phosphate exist is solved.

The invention provides a high performance liquid chromatography detection method for cyanoacetamide. The chromatographic conditions adopted by the method include that: the chromatographic column is Polar-Silica, the particle size is 5 micrometers, the length is 250mm, the diameter is 4.6mm, and the aperture is 120 angstrom; the mobile phase is a mixed system of acetonitrile and methyl chloride; and the detector is ELSD. The detection method provided by the invention has the advantages of rapidity, accuracy, high selectivity, simple sample treatment, and is suitable for qualitative and quantitative detection of cyanoacetamide industrial crude products and intermediates.

The invention relates to a method for measuring bisphenol-S in consumer goods. The measuring method includes the steps that (1) a smashed and evenly mixed sample is taken, an organic solvent is added to the sample, extraction is conducted, an extracted solution is concentrated through a rotary evaporation instrument, and dilution constant volume is conducted on the solution through methanol and water; (2) for a plastic product sample and a wooden product sample, the extracted solution generated after concentration and methanol-water constant volume are conducted is purified through a small HLB pillar; (3) the amount of bisphenol-S in the methanol-water solution obtained through the step (1) or the step (2) is measured through a liquid chromatograph-mass spectrometer; (4) Comparison between the sample and a full scan mass spectrogram of standard matter on the aspects of the retention time, the characteristic ions and the abundance ratio is conducted is conducted, whether the sample contains the bisphenol-S or not is determined, and finally the content of the bisphenol-S in the sample is calculated after conversion according to the amount of the bisphenol-S obtained in the step (3). The measuring method is rapid and simple, the content of the bisphenol-S in the consumer goods made of various materials can be accurately measured, and the detection limits each can reach 0.1 mg / kg.

The invention discloses a sterile dynamic detecting method for tissue culture seedling nitrogen use ratio, which comprises the following steps of: constructing a water loss model and calculating the thickness of culture medium; collecting the diffuse reflection spectrum information in multi-point manner of the culture medium in the modeling sample and the test sample by the near infrared spectrometer to acquire the model of the modeling sample; then forecasting the test sample and acquiring the multipoint nitrogen content in the culture medium of the test sample; carrying out linear fitting by the nitrogen content of a certain point and the vertical distance between the point and the base diameter of the tissue culture seedling to acquire an equation between the nitrogen content and the vertical distance; calculating the total nitrogen amount of the culture medium of the test sample according to cylinder integral formula; detecting the multipoint nitrogen content in the culture mediumof the test sample and detecting the biomass of the tissue culture seedling in the test sample by image processing method at the same time; and finally calculating the nitrogen use ratio of the tissue culture seedling in the test sample according to the formula. The change of nitrogen in the culture medium in the culturing process of plant tissue can be measured in dynamic, sterile and undamaged way without extracting the nitrogen from the culture medium by the sterile dynamic detecting method.

The invention relates to an in-situ microextraction and portable Raman spectrometer combined method for determining polycyclic aromatic hydrocarbon. The method comprises the following steps: performing mercaptan modification on silver nanoparticle surfaces and microextraction on polycyclic aromatic hydrocarbon in a food contact material, condensing the prepared silver nanoparticle solution modified with mercaptan on the surface to be 10-100 folds, then mixing the concentrated solution with the extract solution in situ according to a volume ratio of the concentrated solution to the extract solution being (1-2):(1-2), and adopting the Raman spectrometer to detect surface enhancement Raman spectroscopy signals of polycyclic aromatic hydrocarbon, so as to obtain the polycyclic aromatic hydrocarbon surface enhancement Raman spectroscopy signals. According to the invention, the extraction method and the surface enhancement Raman spectroscopy method are combined, the extracting solution is not required to be transferred into a detector, but the pollutant can be detected in situ, so that the sample pretreatment procedure can be avoided and the error introduced during the pretreatment process can be avoided; the determination method is simple, environment-friendly, rapid and capable of solving the problem that the food contact material is required to be screened rapidly on the site to prevent migration of polycyclic aromatic hydrocarbon into the food.

The invention belongs to the technical field of analysis chemistry and particularly relates to a method for measuring calcium stearate in a drug. The method comprises the following steps of: 1) extracting a sample solution, adding hydrochloric acid to the sample solution to acidize the sample solution, extracting through normal hexane to obtain a normal hexane extracting solution, rotationally evaporating and concentrating the normal hexane extracting solution, and blowing to dryness by using nitrogen; 2) carrying out methyl esterifying, namely, adding methanol into the solution to dissolve, adding sulfuric acid, uniformly mixing, and extracting a methyl esterified product through normal hexane, helically and uniformly mixing, stewing for layering and extracting the normal hexane layer; 3) measuring the dosage of the methyl stearate in the normal hexane layer obtained in step 2) through a gas chromatograph-mass spectrometer; and 4) finally calculating the content of calcium stearate in the sample solution according to the dosage of the methyl stearate obtained in step 3). The method disclosed by the invention is used for measuring calcium stearate in the drug through the gas chromatograph-mass spectrometer by acidizing the sample to extract stearic acid; and the measuring method is not only quick and simple, but also capable of being used for accurately measuring the content of calcium stearate in the drug, and the detection limit is low.

The invention discloses a method for determination of padimate in cosmetics, wherein the method includes the following steps: (1) pretreating samples; (2) analyzing padimate by high performance liquid chromatography, and optimizing high performance liquid chromatography conditions; and (3) drawing a standard working curve, quantifying by adopting an external standard method, and calculating the content of padimate. The method for determination of padimate in the cosmetics adopts the high performance liquid chromatography for simultaneous determination of padimate A and padimate O in the cosmetics; the samples detected to be positive by the high performance liquid chromatography are further identified by using high performance liquid chromatography-mass spectrometry / mass spectrometry. The method is accurate and fast, is high in sensitivity, and has a great significance for monitoring of the cosmetics quality safety risks.

The invention provides a method for rapidly determining the content of a flame retardant in inorganic flame-retardant cellulose fibers. The content of the flame retardant in the inorganic flame-retardant cellulose fibers is rapidly determined by adopting a low-field pulse nuclear magnetic resonance method. The rapid determination method established on the basis of the low-field pulse nuclear magnetic resonance technology has the advantages of simplicity in operation, short test time, high precision, good stability and accurate and reliable measurement result, the quantity of samples required by the test is small, the morphological structure of the samples cannot be damaged in the test process, the samples can be recycled, and resources are saved; the determination method disclosed by the invention is rapid, accurate and good in reproducibility, can be used for simultaneously determining the moisture content and the flame retardant content of an unknown flame-retardant fiber sample at one time, has good conformity between detection data and data obtained by a conventional laboratory determination method, can meet the requirement of rapid analysis and detection of the flame retardant content of a flame-retardant cellulose fiber product, and has good practicability.

The invention discloses a determination method which determines the reduction degree of a cadmium-copper reduction post in water nitrate by continuous flowing automatic chemistry analysis instrument; the basic technical route is that: firstly, nitrite analysis module of multi-channel continuous flowing automatic chemistry analysis instrument is used to analyze nitrite standard series; secondly, nitrate analysis module is used to analyze nitrate standard series; thirdly, nitrate analysis module is used to analyze nitrite standard series; finally, the reduction degree of the cadmium-copper reduction post is determined by slope combination.

The invention discloses a method for rapid determination of feruloyltyramine content in Pothos chinensis, and the method is based on high performance liquid chromatography technology for determination of feruloyltyramine content in Pothos chinensis. The rapid determination is simple and accurate, has high sensitivity, good precision, good stability, and is very conducive to application in rapid quality control of Pothos chinensis medicinal material in the pharmaceutical process.

The invention belongs to the technical field of analytical chemistry and particularly provides a method for measuring content of multiple azoic coupling components in a dye or pigment simultaneously.The method comprises steps as follows: 1), sample pretreatment: dye or pigment samples are mixed uniformly; if the samples are caked, after the caked samples are ground into powder, the samples are mixed uniformly; then the uniformly mixed samples are put in a clean and dry self-sealing bag, sealed and stored for standby application; 2), sample extraction: an appropriate quantity of samples are weighed and ultrasonically extracted by alkaline methanol, and a sample extracting solution is obtained; 3), measurement: concentration of each azoic coupling component in the sample extracting solutionobtained in step 2) is measured by a high performance liquid chromatograph; 4), calculation: content of each azoic coupling component in the samples is calculated according to the concentration of each azoic coupling component in the sample extracting solution obtained by detection in step 3). Compared with the prior art, the measurement method is rapid and simple, the extraction is simple and convenient, and furthermore, separation of multiple azoic coupling components and accurate measurement of the content can be realized.