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Sterile dynamic detecting method for tissue culture seedling nitrogen use ratio

A technology for dynamic detection and tissue culture seedlings, applied in the field of plant biology

Inactive Publication Date: 2012-04-11
YUNNAN JIANGSHI TECH CO LTD
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Problems solved by technology

And the technical scheme that first utilizes near-infrared spectroscopic analysis to measure the nitrogen content in the semi-solid medium of plant tissue culture, and then combines the biomass detection to measure the nitrogen utilization rate of tissue culture seedlings has not been reported.

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  • Sterile dynamic detecting method for tissue culture seedling nitrogen use ratio
  • Sterile dynamic detecting method for tissue culture seedling nitrogen use ratio
  • Sterile dynamic detecting method for tissue culture seedling nitrogen use ratio

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Embodiment

[0015] The test samples are the culture medium of the 3rd to 4th generations of subcultured Zhuge Cai, Atractylodes atractylodes, and rapeseed. The tissue-cultured seedlings were respectively inoculated in the medium with three nitrogen levels, and cultivated for 30 days. Different types of tissue-cultured seedlings The initial nitrogen concentration levels in the medium are shown in Table 1 below:

[0016] Table 1 Initial test nitrogen concentration levels in the medium of different kinds of tissue culture seedlings

[0017]

[0018] by t blank medium volume V t and time t Perform a linear fit to obtain a water loss model. See figure 1 , the fitting equation of blank medium volume and culture time is V t =50.005-0.2707 t , the number of samples n=9; where V t for t Volume of time blank medium (ml); t is the culture time (d) of the tissue culture seedlings.

[0019] The diffuse reflectance spectrum information of the culture medium in the modeling sample ...

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Abstract

The invention discloses a sterile dynamic detecting method for tissue culture seedling nitrogen use ratio, which comprises the following steps of: constructing a water loss model and calculating the thickness of culture medium; collecting the diffuse reflection spectrum information in multi-point manner of the culture medium in the modeling sample and the test sample by the near infrared spectrometer to acquire the model of the modeling sample; then forecasting the test sample and acquiring the multipoint nitrogen content in the culture medium of the test sample; carrying out linear fitting by the nitrogen content of a certain point and the vertical distance between the point and the base diameter of the tissue culture seedling to acquire an equation between the nitrogen content and the vertical distance; calculating the total nitrogen amount of the culture medium of the test sample according to cylinder integral formula; detecting the multipoint nitrogen content in the culture mediumof the test sample and detecting the biomass of the tissue culture seedling in the test sample by image processing method at the same time; and finally calculating the nitrogen use ratio of the tissue culture seedling in the test sample according to the formula. The change of nitrogen in the culture medium in the culturing process of plant tissue can be measured in dynamic, sterile and undamaged way without extracting the nitrogen from the culture medium by the sterile dynamic detecting method.

Description

technical field [0001] The invention relates to a method for aseptic dynamic detection of nitrogen utilization rate of tissue culture seedlings based on spectral analysis method and image processing method, and belongs to the field of plant biotechnology. Background technique [0002] In plant tissue culture, nitrogen plays an important role in promoting plant growth. Nitrogen deficiency directly affects the biosynthesis of amino acids, proteins, nucleic acids and other substances in plants, resulting in the reduction of plant photosynthesis ability and final yield. Its consumption will affect the cultivation Therefore, if the nitrogen utilization rate of tissue culture seedlings can be dynamically detected during the culture process, it will be convenient for studying the physiological properties of cultures, judging the best inoculation period of cultures, and discussing tissue culture plantlets. It is of great significance to improve the growth law of the plant, improve t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01B11/00
Inventor 吴沿友鲁珊赵宽朱咏莉付为国李萍萍毛罕平赵玉国
Owner YUNNAN JIANGSHI TECH CO LTD
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