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Method for rapid determination of feruloyltyramine content in Pothos chinensis

A rapid determination technology for feruloyl tyramide, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of shallow methods, lack of specificity, lack of quantitative indicators and means, etc., and achieve simple determination methods, good stability and sensitivity high effect

Active Publication Date: 2015-11-25
GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM) +1
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Problems solved by technology

[0003] Shimanzi is a commonly used traditional Chinese medicine of the Yao nationality. At present, only the identification of traits is used as the basis for identification, lack of quantitative indicators and means, and the method is very superficial. Not only lacks specificity, but also it is difficult to accurately evaluate the authenticity and quality of the medicinal materials

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  • Method for rapid determination of feruloyltyramine content in Pothos chinensis
  • Method for rapid determination of feruloyltyramine content in Pothos chinensis
  • Method for rapid determination of feruloyltyramine content in Pothos chinensis

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Embodiment Construction

[0022] The rapid assay method of feruloyl tyramide content in the lime provided by the invention, the method comprises the following steps and conditions:

[0023] (1) Chromatographic conditions: with octadecylsilane bonded silica gel as filler; methanol-0.4% phosphoric acid solution (35:65) as mobile phase; detection wavelength is 318nm; less than 1500;

[0024] (2) Preparation of reference substance solution: take an appropriate amount of feruloyl tyramide reference substance, weigh it accurately, add methanol to make a solution containing 143.6 μg per 1 mL, and obtain it;

[0025] (3) Preparation of the test solution: Take about 2 g of the powder of this product (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered conical flask, add 25 mL of methanol precisely, seal it tightly, weigh it, and ultrasonically treat it ( power 250W, frequency 40kHz) for 30 minutes, let cool, weigh again, make up the lost weight with methanol, shake well, filter, and take ...

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Abstract

The invention discloses a method for rapid determination of feruloyltyramine content in Pothos chinensis, and the method is based on high performance liquid chromatography technology for determination of feruloyltyramine content in Pothos chinensis. The rapid determination is simple and accurate, has high sensitivity, good precision, good stability, and is very conducive to application in rapid quality control of Pothos chinensis medicinal material in the pharmaceutical process.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a rapid determination method for the content of feruloyl tyramide in limes. Background technique [0002] Licorice is the dry whole herb of Pothoschinensis (Raf.) Merr., a plant of the genus Pothoschinensis (Raf.) Merr. in the family Araceae. One of the commonly used medicines. However, since the Yao nationality has no written language of its own, the inheritance and dissemination of Yao nationality's medical knowledge is completely passed on by word of mouth, resulting in the confusion of homonyms and different names in the use of Yao medicines, and the safety and effectiveness of clinical medication are difficult to be guaranteed. , At the same time, it also brings great difficulties to the further development and utilization of Yao medicine and the formulation of quality standards. At present, Shikanzi is only recorded in the "Natio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孙冬梅罗文汇钟鸣毕晓黎刘布鸣陈昭邱宏聪赖茂祥胥爱丽江洁怡
Owner GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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