Method for detecting tanshinone components, and application thereof

A technology of tanshinone and cryptotanshinone, which is applied in the field of medicine and can solve problems such as time lag, simplicity, and quality control

Active Publication Date: 2018-10-16
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 2015 edition of "Chinese Pharmacopoeia", the determination of the content of tanshinones under the item of Compound Danshen Tablets was determined by high performance liquid chromatography to detect tanshinone ⅡA, and the content of tanshinones under the item of Danshen was also determined by high performance liquid chromatography. Or UPLC has a time lag, and it is difficult to quickly, efficiently, and real-time reflect the content changes in the process of extracting and concentrating Salvia miltiorrhiza, and cannot be used for content monitoring in the process of extracting and concentrating Salvia miltiorrhiza.
At present, the control mode of the current Chinese medicine production process in my country basically stays on the traditional empirical control method, which rarely takes into account the changes in the ingredients and their quantities in the process, and the control is too simple to achieve on-line detection and quality of the production process. monitor

Method used

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  • Method for detecting tanshinone components, and application thereof
  • Method for detecting tanshinone components, and application thereof
  • Method for detecting tanshinone components, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Model building and selection

[0092] I. Modeling

[0093] 1. Collection of calibration set samples

[0094] When extracting for the first time, use 4-6 times the amount of 95% ethanol to heat and reflux the salvia miltiorrhiza, keep slightly boiling, filter the extract, and concentrate the filtrate; add 3-5 times the amount of 50% ethanol to the dregs and heat and reflux for the second extraction , keep slightly boiling, filter the extract, recover ethanol from the filtrate, and concentrate the filtrate. Samples were taken every 15 minutes during extraction and every 3 minutes during concentration.

[0095] The extraction and concentration process of Danshen mentioned above is the extraction and concentration process of Danshen when preparing Compound Danshen Tablets, wherein the formula of Compound Danshen Tablets is 450g of Salvia miltiorrhiza: 141g of Panax notoginseng: 8g of borneol.

[0096] 2. Determination of the content of dihydrotanshinone, crypto...

Embodiment 2

[0180] Example 2 A method for rapid determination of tanshinone components in the process of extraction and concentration of salvia miltiorrhiza

[0181] The model selected in Example 1 was used to measure the tanshinone components in the process of extracting and concentrating Danshen.

[0182] 1. Collection of samples to be tested

[0183] The samples in the process of extracting and concentrating Salvia miltiorrhiza were collected, and the process of extracting and concentrating Salvia miltiorrhiza was as described in Example 1.

[0184] 2. Collection of near-infrared spectra of samples to be tested

[0185] The near-infrared spectrum of the sample to be tested was measured by a near-infrared spectrometer, and the measurement method of the near-infrared spectrum was as described in Example 1.

[0186] 3. Detection

[0187]The following model established in Example 1 is used to convert the near-infrared spectrum obtained in Part 2 into the content values ​​of dihydrotan...

Embodiment 3

[0192] Example 3 A method for judging the reaction end point of salvia miltiorrhiza extraction and concentration, the method comprises the following steps:

[0193] (1) The method of Example 2 was used to detect the content values ​​of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA in the sample to be tested.

[0194] (2) End point judgment

[0195] For the first extraction in the process of extraction and concentration of Danshen, the initial time is 0 minutes in the state of slight boiling, and samples are taken every 15 minutes according to the aforementioned detection method. Dihydrotanshinone, cryptotanshinone, tanshinone I and The content of tanshinone ⅡA is the denominator, and the content of sampling points at different times is calculated as the numerator, see Figure 14 a.

[0196] For the second extraction in the process of extraction and concentration of Danshen, the initial time is 0 minutes in the state of slight boiling, and samples are...

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Abstract

The invention relates to a method for detecting tanshinone components, and an application thereof. The tanshinone components are dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA. The method comprises the following steps: collecting samples to be tested in the extraction and concentration of Salvia miltiorrhiza, collecting the near infrared spectra of the samples to be tested bynear-infrared spectroscopy, and converting the near infrared spectra into the respective content of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA in every sample to be tested according to a selected model. The method has the advantages of fastness in detection of the respective content of the tanshinone components in a compound Salvia miltiorrhiza tablet, simplicity in thepreparation of the samples, and realization of online analysis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for quickly measuring tanshinone components in the extraction and concentration process of salvia miltiorrhiza and a method for judging the reaction end point of extraction and concentration of salvia miltiorrhiza by using the detection method. Background technique [0002] Near Infrared Spectroscopy (NIR for short) analysis technology is a testing technology developed rapidly in the late 1980s. It is about 12000~4000cm -1 ) characteristic spectrum, and use appropriate chemometric methods to extract relevant information, it is mainly used as a detection technology for qualitative and quantitative analysis of organic substances. Irradiate the measured sample with near-infrared light, use organic matter containing OH, NH and other chemical bonds to vibrate or rotate over-frequency, obtain the absorption spectrum in the near-infrared region by means of diffuse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3577G01N30/02
CPCG01N21/3577G01N21/359G01N30/02
Inventor 唐海姣匡艳辉王德勤黄雁利幼
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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