HPLC (high performance liquid chromatography) detection method for stilbene fluorescent brightening agent and application of HPLC detection method

A high-performance liquid chromatography and fluorescent whitening agent technology, which is applied in the field of detection of stilbene-type fluorescent whitening agents, can solve the problems of fluorescent whitening agents that are difficult to degrade naturally, not recommended for manufacturers to use, and environmental burdens, and achieve repeatability And the effect of good stability, low detection limit and strong applicability

Inactive Publication Date: 2018-07-20
SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2015, the EU Joint Research Center (JRC) pointed out that some fluorescent whitening agents are difficult to degrade naturally when revising the EU eco-label product certification principles, which will cause a burden to the environment, and manufacturers are not recommended to use them in products
At present,

Method used

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  • HPLC (high performance liquid chromatography) detection method for stilbene fluorescent brightening agent and application of HPLC detection method
  • HPLC (high performance liquid chromatography) detection method for stilbene fluorescent brightening agent and application of HPLC detection method
  • HPLC (high performance liquid chromatography) detection method for stilbene fluorescent brightening agent and application of HPLC detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Determination of the optimal detection parameters of the high performance liquid chromatography detection method.

[0042] The present invention determines the optimal detection parameters of the high-performance liquid chromatography detection method by optimizing the sampling volume, demulsification means, ultrasonic extraction time and optimizing the conditions such as chromatographic ion pair reagent, mobile phase ratio, and chromatographic column in the pretreatment step, specifically as Table 1 shows. Each group of embodiments in Table 1 is carried out under the following conditions: the detection range of diode array detector is 210nm-400nm; the excitation wavelength of fluorescence detector is 350nm, and the emission wavelength is 432nm.

[0043] Table 1 Determination of high performance liquid chromatography conditions

[0044]

[0045]

[0046]

[0047]

[0048] Conclusion: The optimal chromatographic conditions for the qualitative and q...

Embodiment 2

[0059] The detection of embodiment 2 sample

[0060] Detection instrument: high performance liquid chromatography with series diode array detector and fluorescence detector. The detection range of the diode array detector is 210nm-400nm, the excitation wavelength of the fluorescence detector is 350nm, and the emission wavelength is 432nm.

[0061] Chromatographic conditions: the chromatographic column is Shiseido MG C 18 Column (4.6mm×250mm, 5μm), mobile phase is acetonitrile-10mmol / L ammonium acetate containing 2mmol / L di-n-hexylammonium acetate (adjust pH to 6.0 with acetic acid), gradient elution (0-12min acetonitrile content maintains 35 %, the content of acetonitrile increases linearly from 35% to 50% in 12-24min, and the content of acetonitrile decreases linearly from 50% to 35% in 24-25min, and finally the content of acetonitrile remains at 35% until 35min), flow rate 1.0mL / min, column temperature 40 ℃, the injection volume is 20 μL.

[0062] Cosmetics: Weigh about 1...

Embodiment 3

[0064] The establishment of embodiment 3 standard curve

[0065] Accurately weigh 20.0 mg of each fluorescent whitening agent standard sample into a 100 mL brown volumetric flask, and make a standard solution (200 μg / mL) with 33% acetonitrile to make a standard solution (200 μg / mL). 2, 10, 50, 100 μg / mL standard series solution, then carry out high performance liquid chromatography detection, adopt the best chromatographic condition and detection instrument of embodiment 1. The result shows: the concentration of each fluorescent whitening agent has a linear relationship with the chromatographic peak area, and the linear regression equation and the correlation coefficient R 2 , see Table 2.

[0066] Table 2 Linear Data

[0067]

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Abstract

The invention discloses an HPLC (high performance liquid chromatography) detection method for a stilbene fluorescent brightening agent. A standard curve is established on the basis of HPLC and combined use of a diode array detector and a fluorescence detector; according to the HPLC, Shiseido MG C18 column is taken as a chromatographic column, acetonitrile and 10 mmol/L ammonium acetate which contains 2 mmol/L dihexylammonium acetate as a mobile phase, the initial volume ratio of acetonitrile to 10 mmol/L ammonium acetate containing 2 mmol/L dihexylammonium acetate is 33:67, and gradient elution is adopted for separation. Composition and content of the stilbene fluorescent brightening agent in cosmetics and detergents can be determined rapidly with the HPLC detection method.

Description

technical field [0001] The invention belongs to the technical field of detection of stilbene-type fluorescent whitening agents, and in particular relates to a high-performance liquid chromatography detection method and application of stilbene-type fluorescent whitening agents in cosmetics and detergents. Background technique [0002] Fluorescent whitening agent belongs to a branch of the dye industry. It is a separate category on the "Colour Index" (C.I.). Its special chemical structure enables it to absorb light with a wavelength of 300-400nm invisible to the naked eye Ultraviolet rays, and then excite the blue or blue-violet fluorescence with a wavelength of 420-480nm visible to the naked eye. This fluorescence can make up for the lack of blue light in the reflected light of white objects with a yellowish color, and enhance the reflected light in the blue band. , the whiteness and brightness of white objects appear to the naked eye to be improved. Because fluorescent whit...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 顾宇翔杨晋青王磊周泽琳戴彦韵王丁林周耀斌
Owner SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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