The invention relates to a method for extracting total triterpenoids and
monomer ilicin A of enriched Hainan holly leaf. The method is characterized by comprising the following extraction steps that raw materials of Hainan holly leaf is subjected to
reflux extraction by 50 percent to 95 percent of
ethanol, liquid supernatant and a
precipitation part are obtained after the extraction liquid is concentrated and centrifuged, the
precipitation part is subjected to mixed suspension by 30 percent to 40 percent of
ethanol, then,
petroleum ether is used for extraction, the lower layer solution is subjected to
pressure reduction, concentration and
drying to obtain total triterpenoids; in addition, the
ethanol extraction liquid is subjected to
pressure reduction,
recovery and
evaporation to
dryness, water is used for dissolving, acid is added for
hydrolysis, organic solvents are extracted, the
recovery to
dryness, the silicagel column
coating and the sherwood oil-
ethyl acetate gradient elution are carried out, eluant is collected,
elution solvents are recovered through
pressure reduction, and the
monomer ilicin A is obtained. According to an extraction enriching method, the total triterpenoids content of the obtained product exceeds 50 percent, the purity of the
monomer ilicin A is high, meanwhile, the
repeatability and the stability of the method are good, the transfer rate of effective ingredients of the
raw material is high, the cost is low, the operation is simple and convenient, and the method is suitable for industrial production. Meanwhile, the invention also relates to application of the total triterpenoids and the monomer ilicin A of Hainan holly leaf in the cardiovascular
disease treating aspect, in particular to the atherosclerosis aspect, and the potential development value is realized.