Enzyme-linked immunologic detection method of bisphenol A

An enzyme-linked immunoassay and bisphenol technology, applied in measuring devices, material analysis by observing the impact on chemical indicators, instruments, etc., to achieve high specificity and high affinity, high selectivity and sensitivity, The effect of low cost

Inactive Publication Date: 2009-04-22
JIANGNAN UNIV
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Problems solved by technology

But so far, there is no better report on the immunological detection method for bisphenol structure residues in China. For this reason, a

Method used

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  • Enzyme-linked immunologic detection method of bisphenol A

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[0049] Specific implementation plan

[0050] The following examples further illustrate the present invention.

[0051] 1. Instrument:

[0052] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory;

[0053] KFLOW pure water machine, Kevron Company;

[0054] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory;

[0055]Costar 96-well 8×12 detachable ELISA plate, Shanghai Jitai Biotechnology Co., Ltd.;

[0056] MuLtiska Mks microplate reader, Thermo Labsystems;

[0057] Adjustable pipette, Thermo Labsystems;

[0058] Vortex mixer, Shanghai Huxi Instrument Analysis Factory.

[0059] 2. Reagents:

[0060] Horseradish peroxidase labeled goat anti-rabbit IgG (GAR-HRP), Kangcheng Bioengineering Company;

[0061] Tetramethylbenzidine (TMB), Huamei Bioengineering Company;

[0062] Other reagents are analytical reagents.

[0063] Three, steps

[0064] 1. Synthesis of immunogen and coating

[0065] The synthetic immunogen (bisphenolic acid ...

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Abstract

An enzyme-linked immunoassay method of bisphenol A belongs to the technical field of immunodetection. The enzyme-linked immunosorbent assay method utilizes the immunization of a synthetic bisphenol A immunogen to obtain a polyclonal antibody, takes the bisphenol A as a standard product, takes a conjugate of a hapten of diphenolic acid and OVA as a coating antigen and establishes the indirect competitive enzyme-linked immunosorbent assay method of the bisphenol A. The enzyme-linked immunosorbent assay method establishes the indirect competitive ELISA method of the bisphenol A and provides a rapid and high-efficient detection method for detecting the residual bisphenol A, as the method adopts the polyclonal antibody, the cost is lower, and the stability and the repeatability are good. The sensitivity is 0.1ng/ml, and the linear range is 0-100ng/ml. The high specificity and the affinity of the immune reaction lead the ELISA to have very high selectivity and sensitivity.

Description

technical field [0001] The invention relates to an alkylphenol residue detection method, more specifically an enzyme-linked immunological detection method for bisphenol A, belonging to the technical field of immunological detection. Background technique [0002] Bisphenol A (Bisphenol A, BPA) is a kind of "Endocrine Disrupting Chemicals (ECD)" newly discovered from food packaging materials in recent years, which has certain estrogenic properties. The discovery of BPA has aroused widespread concern for food and packaging materials in various countries. There have been reports of detection of BPA from canned vegetables, baby preparations, and food cans. BPA is the main raw material for the production of PC resin and epoxy (EP) resin, and is also used in phenolic resin, plastic polyester, antioxidant and polyvinyl chloride (PVC) stabilizer. Therefore, it has important uses in food packaging materials, container inner wall coatings, etc. In professional production and daily li...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/78
Inventor 胥传来李灼坤马伟徐丽广
Owner JIANGNAN UNIV
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