Preparation method of ilicin A and total triterpenoids contained in Hainan holly leaf and application of ilicin A and total triterpenoids

A technology of mountain green tea and total triterpenes, which is applied to the method for extracting and enriching monomers of total triterpenes and ilextin A of mountain green tea and the field of cardiovascular activity thereof, can solve the problems such as no single component medicine, achieves low cost, Easy to operate, reproducible and stable results

Inactive Publication Date: 2013-01-16
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, statins are the first choice for clinical lipid-lowering treatment. There are few reports on the efficacy evaluation of traditional Chinese medicine for anti-atherosclerosis, and there are

Method used

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  • Preparation method of ilicin A and total triterpenoids contained in Hainan holly leaf and application of ilicin A and total triterpenoids
  • Preparation method of ilicin A and total triterpenoids contained in Hainan holly leaf and application of ilicin A and total triterpenoids
  • Preparation method of ilicin A and total triterpenoids contained in Hainan holly leaf and application of ilicin A and total triterpenoids

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Experimental program
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Example Embodiment

[0025] Example 1

[0026] a. Take 5kg of raw camellia, add 25L of 70% methanol solution and soak overnight, heat and reflux for extraction once, 1.5h each time;

[0027] b. Combine the extracts, concentrate to obtain a thick extract, centrifuge at 4000r / min for 10min, and separate the supernatant and the precipitated part;

[0028] c. Suspend the precipitated part with 30%-40% ethanol, and then extract with petroleum ether (5:1, v / v) for 3 times to decolorize. The lower layer solution is concentrated under reduced pressure to obtain the extract, which is naturally dried, crushed, and passed. A 60-mesh sieve yielded 537g of total triterpenoids in green tea with a content of 50.8%.

Example Embodiment

[0029] Example 2

[0030] a. Take 5kg of raw camellia, add 35L of 75% ethanol solution to soak overnight, heat and reflux for extraction once, 3h each time;

[0031] b. Combine the extracts, concentrate to obtain a thick extract, centrifuge at 4000r / min for 10min, and separate the supernatant and the precipitated part;

[0032] c. Suspend the precipitated part with 30%-40% ethanol, and then extract twice with petroleum ether (3:1, v / v) to decolorize. The lower layer solution is concentrated under reduced pressure to obtain the extract, which is naturally dried, crushed, and passed. A 60-mesh sieve yielded 546 g of total triterpenoids in green tea with a content of 53.6%.

Example Embodiment

[0033] Example 3

[0034] a. Take 5kg of raw camellia, add 50L of 85% ethanol solution to soak overnight, heat and reflux for extraction 2 times, 2.5h each time;

[0035] b. Combine the extracts, concentrate to obtain a thick extract, centrifuge at 4000r / min for 10min, and separate the supernatant and the precipitated part;

[0036] c. Suspend the precipitated part with 30%-40% ethanol, and then extract 3 times with dichloromethane (6:1, v / v) to decolorize. The lower layer solution is concentrated under reduced pressure to obtain the extract, which is naturally dried and crushed. After passing through a 60-mesh sieve, 562 g of total triterpenoids of mountain green tea were obtained, with a content of 54.7%.

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Abstract

The invention relates to a method for extracting total triterpenoids and monomer ilicin A of enriched Hainan holly leaf. The method is characterized by comprising the following extraction steps that raw materials of Hainan holly leaf is subjected to reflux extraction by 50 percent to 95 percent of ethanol, liquid supernatant and a precipitation part are obtained after the extraction liquid is concentrated and centrifuged, the precipitation part is subjected to mixed suspension by 30 percent to 40 percent of ethanol, then, petroleum ether is used for extraction, the lower layer solution is subjected to pressure reduction, concentration and drying to obtain total triterpenoids; in addition, the ethanol extraction liquid is subjected to pressure reduction, recovery and evaporation to dryness, water is used for dissolving, acid is added for hydrolysis, organic solvents are extracted, the recovery to dryness, the silicagel column coating and the sherwood oil-ethyl acetate gradient elution are carried out, eluant is collected, elution solvents are recovered through pressure reduction, and the monomer ilicin A is obtained. According to an extraction enriching method, the total triterpenoids content of the obtained product exceeds 50 percent, the purity of the monomer ilicin A is high, meanwhile, the repeatability and the stability of the method are good, the transfer rate of effective ingredients of the raw material is high, the cost is low, the operation is simple and convenient, and the method is suitable for industrial production. Meanwhile, the invention also relates to application of the total triterpenoids and the monomer ilicin A of Hainan holly leaf in the cardiovascular disease treating aspect, in particular to the atherosclerosis aspect, and the potential development value is realized.

Description

technical field [0001] The invention relates to an extraction and enrichment process of effective parts and monomers of traditional Chinese medicines and its application, in particular to a method for extraction and enrichment of total triterpenes and hollylicin A monomers of camellia green tea and their cardiovascular activity, belonging to the technical field of traditional Chinese medicines. Background technique [0002] Cardiovascular disease is a common clinical disease. In recent years, with the rapid economic development and continuous improvement of living standards, especially the changes in diet structure and living habits, the incidence and mortality of cardiovascular diseases in the world have continued to increase. Atherosclerosis is a type of arteriosclerosis, manifested by the appearance of yellow substances containing cholesterol and fat-like substances in the intima of large and medium arteries, mostly caused by disorders of fat metabolism and neurovascular d...

Claims

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Application Information

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IPC IPC(8): A61K36/185C07J63/00A61P9/00A61P9/10
Inventor 杨杰崔伟曦王强陈筱清谈景福白敏李晶晶
Owner CHINA PHARM UNIV
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