Development and application of molecular markers related to F-type three-line hybrid wheat male sterility gene
A technology of male sterility gene and hybrid wheat, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problem of recovering a wide range of sources, and achieve strong operability and simple methods Good, specific effect
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[0049] The acquisition of SNPs related to male sterility in F-type three-line hybrid wheat and the development of molecular markers include the following steps:
[0050] (1) Through 3 rounds of crossing, 2 rounds of backcrossing, 8 generations of sister crossing and 6 generations of test crossing with 4 common wheat varieties, a batch of stable homozygous new sterile line FA and maintainer FB lines were bred (selected See National Invention Patent CN200810135045 for the breeding method), wherein the self-seed setting rate of FA-mix (international law) is 2.9%, the self-seed setting rate of FB-4 (international law) is 137.5%, and the self-seed setting rate of FB-5 (international law) ) is 66.7%.
[0051] (2) Genomic DNA of the above three samples was extracted by the CTAB method (cetyltrimethylammonium Ammonium Bromide) and randomly interrupted. After agarose electrophoresis, the required DNA fragments were recovered and adapters were added and carried out. Cluster preparation...
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