Development and application of molecular markers related to F-type three-line hybrid wheat male sterility gene

A technology of male sterility gene and hybrid wheat, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problem of recovering a wide range of sources, and achieve strong operability and simple methods Good, specific effect

Pending Publication Date: 2019-09-03
BIOCENTURY TRANSGENE CHINA
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Problems solved by technology

FA is a newly discovered source of sterility in my country. It has the advantages of no exogenous cytoplasmic toxicity and a wide range of recovery sources. However, the basic research on

Method used

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  • Development and application of molecular markers related to F-type three-line hybrid wheat male sterility gene
  • Development and application of molecular markers related to F-type three-line hybrid wheat male sterility gene
  • Development and application of molecular markers related to F-type three-line hybrid wheat male sterility gene

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Embodiment 1

[0049] The acquisition of SNPs related to male sterility in F-type three-line hybrid wheat and the development of molecular markers include the following steps:

[0050] (1) Through 3 rounds of crossing, 2 rounds of backcrossing, 8 generations of sister crossing and 6 generations of test crossing with 4 common wheat varieties, a batch of stable homozygous new sterile line FA and maintainer FB lines were bred (selected See National Invention Patent CN200810135045 for the breeding method), wherein the self-seed setting rate of FA-mix (international law) is 2.9%, the self-seed setting rate of FB-4 (international law) is 137.5%, and the self-seed setting rate of FB-5 (international law) ) is 66.7%.

[0051] (2) Genomic DNA of the above three samples was extracted by the CTAB method (cetyltrimethylammonium Ammonium Bromide) and randomly interrupted. After agarose electrophoresis, the required DNA fragments were recovered and adapters were added and carried out. Cluster preparation...

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Abstract

The invention discloses a method for development of specific PCR markers of SNP loci related to an F-type three-line hybrid wheat male sterility gene, wherein the method comprises the steps: (1) carrying out multi-generation sister crossing on wheat, dividing the obtained selfing lines into a maintainer line with high self-fruitful rate, a maintainer line with medium self-fertility rate, and a stable sterile line with self-fruitful rate low than 5%, wherein the three lines are identical in other biological characteristics except having fertility difference; (2) carrying out library-building and sequencing of the three samples; (3) searching for SNP variations between mitochondrial and cell nucleus genomes of the three samples; and (4) aiming at mitochondrial and cell nucleus genome SNP loci obtained after identification of the sterile line and the maintainer lines with different fruitfulness rates, detecting, designing and developing restriction endonuclease amplified polymorphism sequence (CAPS) markers or competitive allele specific PCR (KASP) markers. The invention also discloses relevant SNP loci molecular markers and a detection method thereof.

Description

technical field [0001] The invention belongs to the field of wheat molecular breeding, in particular, it relates to the resequencing analysis of mitochondrial genome and nuclear genome of common wheat sterile lines and two maintainer lines, and at the same time relates to the development of molecular markers related to F-type male sterility gene, and related molecular markers in The application of F-type three-line hybrid wheat sterile line improvement breeding to improve the efficiency of F-type hybrid wheat molecular breeding improvement. Background technique [0002] Wheat is the world's largest and my country's second largest food crop. Wheat production is an important agricultural industry that is related to my country's national economy, people's livelihood and social stability. Compared with crops such as rice, corn, and rapeseed, wheat is one of the crops that have not been widely used in production so far. As a worldwide problem, hybrid wheat has always been highly...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/6858C12Q1/6895C12N15/11
CPCC12Q1/6869C12Q1/6858C12Q1/6895C12Q2600/156C12Q2600/13C12Q2535/122C12Q2531/113C12Q2521/301
Inventor 吴坤杨雅生冯树英
Owner BIOCENTURY TRANSGENE CHINA
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