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Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof

A technology of acetylcholinesterase and biosensor, applied in chitosan-immobilized acetylcholinesterase electrochemical biosensor and application field, can solve the problems of leakage, acetylcholinesterase easy to fall off, etc., achieve long life, not easy to fall off, good The effect of repeatability and stability

Inactive Publication Date: 2009-09-09
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, studies have shown that the disadvantage of using PDDA compared to chitosan is that acetylcholinesterase is easier to fall off and leak

Method used

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  • Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof
  • Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof
  • Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof

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Experimental program
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Embodiment

[0045] Preparation of chitosan-immobilized acetylcholinesterase electrochemical biosensor, the schematic diagram of the preparation process is shown in figure 1 . Concrete preparation steps are as follows:

[0046] (1) The glassy carbon electrode is pretreated by polishing and electrochemical activation by cyclic voltammetry scanning for 10 laps in a pH7.40 phosphate buffer solution;

[0047] (2), the pretreated glassy carbon electrode and platinum electrode in step (1) are used to form a two-electrode system and electrodeposited for 2 hours to form a bright blue electrodeposited layer of carbon nanotubes with a large amount of negative charges;

[0048] (3), immerse the glassy carbon electrode after electrodeposition in step (2) into disperse with the acetic acid solution of 2mol / L, and regulate in the 0.5% chitosan solution that pH value is 5.0 with sodium hydroxide, carry out For the electrostatic self-assembly of chitosan, take it out after 15 minutes and carefully rins...

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Abstract

The invention relates to an electrochemical biosensor and application thereof, in particular to an electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof. The acetylcholinesterase is immobilized with chitosan by way of layer-by-layer self-assembly, but not immobilized by simple cross-linking. The biosensor detects organic phosphorus and carbamate pesticides at a higher sensitivity, and a prepared enzyme electrode is not easy to fall off has better repeatability, better stability and longer service life.

Description

technical field [0001] The present invention relates to a kind of electrochemical biosensor and application, especially Chitosan immobilization Acetylcholinesterase electrochemical biosensor and its application. Background technique [0002] At present, there are many methods for pesticide detection, including gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry, enzyme inhibition, immunoassay, and biosensor. Chromatography involves relatively complicated pretreatment and requires high equipment; the disadvantage of enzyme inhibition method is that the determination of samples and pesticide types is limited. Currently, it is only used for the detection of organophosphorus and carbamate pesticide residues in vegetables and fruits, and cannot give Qualitative and quantitative results can only be used as a qualitative and rapid primary screening method; due to the wide variety of pesticides, the difficulty of antibody preparation, ...

Claims

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Application Information

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IPC IPC(8): G01N27/327A01N57/00A01N47/10
Inventor 张淑平郑炜赵彦马杰
Owner UNIV OF SHANGHAI FOR SCI & TECH
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