Electrochemical biosensor based on chitosan-immobilized acetylcholinesterase and application thereof
A technology of acetylcholinesterase and biosensor, applied in chitosan-immobilized acetylcholinesterase electrochemical biosensor and application field, can solve the problems of leakage, acetylcholinesterase easy to fall off, etc., achieve long life, not easy to fall off, good The effect of repeatability and stability
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[0045] Preparation of chitosan-immobilized acetylcholinesterase electrochemical biosensor, the schematic diagram of the preparation process is shown in figure 1 . Concrete preparation steps are as follows:
[0046] (1) The glassy carbon electrode is pretreated by polishing and electrochemical activation by cyclic voltammetry scanning for 10 laps in a pH7.40 phosphate buffer solution;
[0047] (2), the pretreated glassy carbon electrode and platinum electrode in step (1) are used to form a two-electrode system and electrodeposited for 2 hours to form a bright blue electrodeposited layer of carbon nanotubes with a large amount of negative charges;
[0048] (3), immerse the glassy carbon electrode after electrodeposition in step (2) into disperse with the acetic acid solution of 2mol / L, and regulate in the 0.5% chitosan solution that pH value is 5.0 with sodium hydroxide, carry out For the electrostatic self-assembly of chitosan, take it out after 15 minutes and carefully rins...
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