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Specific chromatogram construction method and quality detection method of schizonepeta

A quality detection method and characteristic map technology, which is applied in the field of characteristic map construction, can solve the problems of ineffectiveness and safety quality control, etc.

Active Publication Date: 2019-05-17
BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The second technical problem to be solved by the present invention is to overcome the defect that the existing technology cannot carry out comprehensive quality control on Nepeta from two aspects of effectiveness and safety, and establish a quality detection method of Nepeta

Method used

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  • Specific chromatogram construction method and quality detection method of schizonepeta
  • Specific chromatogram construction method and quality detection method of schizonepeta
  • Specific chromatogram construction method and quality detection method of schizonepeta

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Effect test

Embodiment 1

[0122] This embodiment provides a method for constructing a characteristic map of Nepeta chinensis, including a method for constructing a characteristic map of non-volatile components and a method for constructing a characteristic map of volatile components, as follows:

[0123] A, the construction method of described non-volatile component feature map, comprises the steps:

[0124] The preparation of need testing solution I: get and produce 0.5g of Nepeta chinensis medical material powder (crossing the No. 2 sieve) from Anhui, put in the stoppered conical flask, add methanol 25ml, close the plug, weigh the weight, reflux 30 minutes, Let it cool, make up for the lost weight with methanol, shake well, take the supernatant and filter, and take the subsequent filtrate to obtain the product;

[0125] Preparation of reference solution I: Take 0.5 g of Schizonepeta reference drug powder (passed through a No. 2 sieve), put it in a stoppered Erlenmeyer flask, add 25 ml of methanol, se...

Embodiment 2

[0134] This embodiment provides a method for constructing a characteristic map of Nepeta chinensis, including the steps of a method for constructing a characteristic map of non-volatile components and the steps of a method for constructing a characteristic map of volatile components, specifically as follows:

[0135] A, the construction method of described non-volatile component feature map, comprises the steps:

[0136] The preparation of need testing solution I: get and produce in Anhui for testing Schizonepeta herb powder (crossing the No. 2 sieve) 1g, put in the stoppered Erlenmeyer flask, add methanol 15ml, close the plug, weigh, reflux for 60 minutes, put Cool, use methanol to make up the lost weight, shake well, take the supernatant and filter, and take the subsequent filtrate to obtain;

[0137] Preparation of reference solution I: Get 1 g of Schizonepeta reference drug powder (passed through No. 2 sieve), put it in a stoppered Erlenmeyer flask, add 15 ml of methanol, ...

Embodiment 3

[0146] This embodiment provides a method for constructing a characteristic map of Nepeta chinensis, including the steps of a method for constructing a characteristic map of non-volatile components and the steps of a method for constructing a characteristic map of volatile components, specifically as follows:

[0147] A, the construction method of described non-volatile component feature map, comprises the steps:

[0148] The preparation of need testing solution I: get the Nepeta decocting pieces powder (crossing the No. 2 sieve) 0.8g produced in Hebei, put in the stoppered conical flask, add ethanol 30ml, close plug, weigh, reflux 120 minutes, Let it cool, make up the lost weight with ethanol, shake well, take the supernatant and filter, and take the subsequent filtrate to get the product;

[0149] Preparation of reference solution I: Get 0.8 g of Schizonepeta reference medicine powder (passed through a No. 2 sieve), put it in a stoppered Erlenmeyer flask, add 30 ml of ethanol...

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Abstract

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a specific chromatogram construction method and a quality detection method of schizonepeta. Liquid chromatography conditions, mobile phase compositions and an elution procedure are controlled, and a specific chromatogram of nonvolatile constituents of the schizonepeta is established; suitable gas chromatography conditions are selected, and a specific chromatogram of volatile constituents of the schizonepeta is established; specific peaks of the specific chromatograms are marked; the liquidchromatography elution procedure is adjusted, and the content of hesperidin in the schizonepeta is determined; and at the same time, a quality control method of the schizonepeta is established, the quality control method comprises the steps that the schizonepeta specific chromatograms are used for evaluating the schizonepeta, the content of the hesperidin is determined, the quality control methodfurther comprises the steps that thin layer chromatography identification is conducted, heavy metal and hazardous elements are examined, the pesticide residue amount is determined, extraction determination is conducted, and the quality of the schizonepeta is evaluated from the perspective of effectiveness and safety. The quality detection method has the advantages of being simple, fast, stable, reliable, high in precision, good in reproducibility, easy to master, and the like.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a construction method and a quality detection method of a nepeta nepeta characteristic map. Background technique [0002] Schizonepeta, mild in nature, pungent in taste, has the effects of relieving exterior wind, clearing rash and eliminating sores, and is used for colds, headaches, measles, rubella and sores, and is one of the commonly used traditional Chinese medicines. In today's increasingly concerned about the quality of medicines, the quality control of Chinese herbal medicines is very important. With the development of chromatographic technology, the quality standard of Nepeta chinensis includes not only simple properties, identification, routine inspection and determination of extracts, but also the determination of HPLC fingerprint. Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification, and i...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74G01N30/86G01N30/88G01N30/90
Inventor 迟玉明姚璐王淑英储兆铮李铮颜士华
Owner BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D
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