Method for detecting HPLC specific chromatogram of origin-preserving decoction

A detection method and feature map technology, applied in the field of feature map detection, can solve problems such as the lack of quality control methods for Baoyuan Decoction, and achieve effective quality evaluation and control, high precision and accuracy, and good stability and repeatability. Effect

Active Publication Date: 2020-03-24
XIAMEN TRADITIONAL CHINESE MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a detection method for the characteristic spectrum of Baoy

Method used

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  • Method for detecting HPLC specific chromatogram of origin-preserving decoction
  • Method for detecting HPLC specific chromatogram of origin-preserving decoction
  • Method for detecting HPLC specific chromatogram of origin-preserving decoction

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1: Preparation method of Baoyuan soup

[0023] Ginseng 3.73g Astragalus 7.46g Licorice 1.87g Cinnamon 0.75g Ginger 3.00g

[0024] Take 5 flavors, soak in water for 0.5 hour, decoct twice, one decoction with 600ml of water and decoction for 45 minutes; the second decoction with 450ml of water and decoction for 35 minutes; the decoction is filtered while it is hot, the filtrate is combined and concentrated to about fifth 1. Freeze-dry into freeze-dried powder, ready to be obtained.

Example Embodiment

[0025] Example 2: Detection method of Baoyuantang feature map

[0026] 1. Instruments and reagents

[0027] Instrument: Agilent 1260 high performance liquid chromatograph (with Agilent 1290DAD detector); Sartorious M-power one-tenth electronic balance and Sartorious TE212-L one-percent electronic balance (Sartorious Scientific Instruments (Beijing) Co., Ltd. ), XS105DU 1 / 100,000 electronic balance (METTLER TOLEDO, Switzerland); KQ-500E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DK-98-ⅡA electric heating constant temperature water bath (Tianjin Test Instruments Co., Ltd.).

[0028] Reagents: ginsenoside Rg1 reference substance (batch number: 110703-201731), ginsenoside Re reference substance (batch number: 110754-201827), ginsenoside Rb1 (batch number: 110704-201726) were purchased from China Institute for Food and Drug Control; Chromatography Grade acetonitrile (United States, Fisher Company), water (Wahaha purified water).

[0029] 2. Preparation of test solution...

Example Embodiment

[0039] Example 3: Investigation of multiple batches of samples of Baoyuantang feature map

[0040] Using decoction pieces from different origins, 18 batches of Baoyuantang freeze-dried powder were obtained according to the preparation method in Example 1, and 18 batches of Baoyuantang liquid chromatogram data (AIA data) were imported into the "Chinese Medicine Chromatographic Feature Atlas Similarity Evaluation System" (2012 Version) software, with S1 as the reference map, the time window width is set to 0.1min, after multi-point calibration and full spectrum peak matching, the control map is generated by the mean method, and the superimposed map of Baoyuantang sample is shown below figure 2 . The 11 common peaks with large content, good resolution and comparative characteristics in the map are selected as the characteristic map. In the characteristic map, the ginsenoside Rb1 peak has good resolution, suitable time, good stability, and is the characteristic active component of g...

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Abstract

The invention discloses a method for detecting an HPLC specific chromatogram of an origin-preserving decoction. The method is characterized by comprising the following steps of (1) preparing a test solution, namely taking an origin-preserving decoction preparation to be detected, and preparing the test solution; (2) preparing a mixed reference substance solution, namely taking ginsenosides Rg1, Reand Rb1, and preparing the ginsenosides Rg1, Re and Rb1 into the mixed reference substance solution; and (3) formulating a specific chromatogram, namely respectively sucking the test solution and thereference substance solution, injecting the solutions into a high performance liquid chromatograph, and obtaining the specific chromatogram of the origin-preserving decoction according to a common peak in the chromatograms of more than N batches of tested products. The detection method provided by the invention can be used for effectively characterizing the variety and quantity of the chemical components in the origin-preserving decoction, and an effective detection method is provided for the comprehensive and effective quality control of the origin-preserving decoction.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection and quality control, in particular, the invention relates to a detection method and application of a characteristic map of Baoyuan Decoction. Background technique [0002] Baoyuan Decoction comes from "Concise Medicine" (1629) compiled by Ming Sun Zhihong. It is an ancient classic prescription. Baoyuan Decoction can benefit Qi and Yang. It is traditionally used to treat weak vitality, mental fatigue, slow muscles, poor diet, and complexion. White, sleep peacefully, acne can't stand up embolism. Modern clinics are widely used in the treatment of coronary heart disease, chronic renal failure, aplastic anemia, hepatitis B, leukopenia and other diseases. [0003] Traditional Chinese medicine compound preparations are multi-component complex systems, and quality control is difficult. Feature map is a comprehensive and quantifiable quality control method, which can mor...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/34
CPCG01N30/88G01N30/06G01N30/34G01N2030/884
Inventor 孔令梅齐晓丹王春艳刘海滨张淹张路孙阳恩李士栋周祥山刘维岩任汝静
Owner XIAMEN TRADITIONAL CHINESE MEDICINE
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