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Method for detecting HPLC specific chromatogram of origin-preserving decoction

A detection method and feature map technology, applied in the field of feature map detection, can solve problems such as the lack of quality control methods for Baoyuan Decoction, and achieve effective quality evaluation and control, high precision and accuracy, and good stability and repeatability. Effect

Active Publication Date: 2020-03-24
XIAMEN TRADITIONAL CHINESE MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a detection method for the characteristic spectrum of Baoyuan Decoction, to solve the problem of lack of precise quality control methods for Baoyuan Decoction in the prior art

Method used

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  • Method for detecting HPLC specific chromatogram of origin-preserving decoction
  • Method for detecting HPLC specific chromatogram of origin-preserving decoction
  • Method for detecting HPLC specific chromatogram of origin-preserving decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Baoyuan Decoction preparation method

[0023] Ginseng 3.73g Astragalus 7.46g Licorice 1.87g Cinnamon 0.75g Ginger 3.00g

[0024] Take the top 5 flavors, add water to soak for 0.5 hours, decoct twice, add 600ml water to decoct for 45 minutes; add 450ml water to decoct for 35 minutes; 1. Freeze-drying to obtain a freeze-dried powder.

Embodiment 2

[0025] Example 2: Baoyuan Decoction characteristic map detection method

[0026] 1. Instruments and reagents

[0027] Instruments: Agilent1260 high performance liquid chromatography (with Agilent 1290DAD detector); Sartorious M-power one-tenth electronic balance and Sartorious TE212-L one-hundredth electronic balance (Sartorious Scientific Instruments (Beijing) Co., Ltd. ), XS105DU 1 / 100,000 electronic balance (Switzerland, Mettler-Toledo); KQ-500E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); DK-98-ⅡA electric constant temperature water bath (Tianjin Tester Instrument Co., Ltd.).

[0028] Reagent: ginsenoside Rg1 reference substance (batch number: 110703-201731), ginsenoside Re reference substance (batch number: 110754-201827), ginsenoside Rb1 (batch number: 110704-201726) were purchased from China National Institutes for Food and Drug Control; Grade acetonitrile (U.S., Fisher company), water (Wahaha purified water).

[0029] 2. Preparation of the test solu...

Embodiment 3

[0039] Example 3: Inspection of multiple batches of samples of Baoyuan Tang characteristic map

[0040] Using decoction pieces from different origins to feed, according to the preparation method of Example 1, 18 batches of Baoyuantang freeze-dried powder were obtained, and the 18 batches of Baoyuantang liquid chromatogram data (AIA data) were imported into the "Chinese Medicine Chromatography Characteristic Map Similarity Evaluation System" (2012 version) software, with S1 as the reference spectrum, the time window width is set to 0.1min, after multi-point calibration and full-spectrum peak matching, the comparison spectrum is generated by the mean value method, and the overlay spectrum of the Baoyuantang sample is shown below figure 2. The 11 common peaks with large content, good separation and comparative characteristics in the spectrum were selected as the characteristic spectrum. In the characteristic spectrum, the peak separation of ginsenoside Rb1 was good, the time was...

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Abstract

The invention discloses a method for detecting an HPLC specific chromatogram of an origin-preserving decoction. The method is characterized by comprising the following steps of (1) preparing a test solution, namely taking an origin-preserving decoction preparation to be detected, and preparing the test solution; (2) preparing a mixed reference substance solution, namely taking ginsenosides Rg1, Reand Rb1, and preparing the ginsenosides Rg1, Re and Rb1 into the mixed reference substance solution; and (3) formulating a specific chromatogram, namely respectively sucking the test solution and thereference substance solution, injecting the solutions into a high performance liquid chromatograph, and obtaining the specific chromatogram of the origin-preserving decoction according to a common peak in the chromatograms of more than N batches of tested products. The detection method provided by the invention can be used for effectively characterizing the variety and quantity of the chemical components in the origin-preserving decoction, and an effective detection method is provided for the comprehensive and effective quality control of the origin-preserving decoction.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection and quality control, in particular, the invention relates to a detection method and application of a characteristic map of Baoyuan Decoction. Background technique [0002] Baoyuan Decoction comes from "Concise Medicine" (1629) compiled by Ming Sun Zhihong. It is an ancient classic prescription. Baoyuan Decoction can benefit Qi and Yang. It is traditionally used to treat weak vitality, mental fatigue, slow muscles, poor diet, and complexion. White, sleep peacefully, acne can't stand up embolism. Modern clinics are widely used in the treatment of coronary heart disease, chronic renal failure, aplastic anemia, hepatitis B, leukopenia and other diseases. [0003] Traditional Chinese medicine compound preparations are multi-component complex systems, and quality control is difficult. Feature map is a comprehensive and quantifiable quality control method, which can mor...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/34
CPCG01N30/88G01N30/06G01N30/34G01N2030/884
Inventor 孔令梅齐晓丹王春艳刘海滨张淹张路孙阳恩李士栋周祥山刘维岩任汝静
Owner XIAMEN TRADITIONAL CHINESE MEDICINE
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