ELISA detecting method for dihexyl phthalate

A technology of dihexyl phthalate and enzyme-linked immunoassay, which is applied in the field of immunoassay, can solve the requirement that there is no dihexyl phthalate enzyme-linked immunoassay, which cannot meet the requirements and requirements of fast, convenient and accurate Compared with advanced problems, it achieves the effects of simple pretreatment process, high selectivity and sensitivity, good stability and repeatability

Inactive Publication Date: 2009-04-29
JIANGNAN UNIV
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Problems solved by technology

At present, the detection of dihexyl phthalate at home and abroad mainly includes high performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid-mass spectrometry (LC -MS), etc., but these methods not only require expensive instruments and equipment, professional operators, but also have relatively high requirements for test materials, and furth

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  • ELISA detecting method for dihexyl phthalate

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[0051] Specific implementation plan

[0052] The following examples further illustrate the present invention.

[0053] 1. Instrument:

[0054] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory;

[0055] KFLOW water purifier, Kevron Corporation;

[0056] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory;

[0057] Costar 96-well 8×12 detachable enzyme-labeled plate, Shanghai Jitai Biotechnology Co., Ltd.;

[0058] MuLtiska Mks microplate reader, Thermo Labsystems;

[0059] Adjustable pipette, Thermo Labsystems;

[0060] Vortex mixer, Shanghai Huxi Instrument Analysis Factory.

[0061] 2. Reagents:

[0062] Horseradish peroxidase labeled goat anti-rabbit GAR-HRP, Kangcheng Bioengineering Company;

[0063] Tetramethylbenzidine (TMB), Huamei Bioengineering Company;

[0064] Other reagents are analytical reagents.

[0065] Three, steps

[0066] 1. Synthesis of immunogen and coating

[0067] Synthetic immunogen (a conjugate of dihe...

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Abstract

The invention relates to an enzyme-linked immunoassay method for dihexyl phthalate, and belongs to the technical field of immunodetection. The invention utilizes immunity of synthesized dihexyl phthalate immunogen to obtain a polyclonal antibody, and takes the dihexyl phthalate as standard and conjugate of dihexyl phthalate hapten and OVA as envelope antigen, to establish indirect competitive enzyme-linked immunoassay method for the dihexyl phthalate. The invention establishes the indirect competitive ELISA method for the dihexyl phthalate, and provides a quick and high-efficiency detecting method for detecting residual of the dihexyl phthalate. The method adopts the polyclonal antibody, so the cost is lower and the stability and repetitiveness are good; and high specificity and compatibility of an immunoreaction make the ELISA have extremely high selectivity and sensitivity, and the sensitivity is 0.01ng/mL and the linear range is between 0 and 100ng/mL.

Description

technical field [0001] The invention relates to a method for detecting phthalate residues, in particular to an enzyme-linked immunological detection method for dihexyl phthalate, belonging to the technical field of immunological detection. Background technique [0002] Di-n-Hexyl phthalate (DHP) is a kind of phthalate. Phthalate esters are widely used as plasticizers in the processing and production of plastics, especially in the processing and production of polyvinyl chloride (PVC). Since the phthalate plasticizer and the plastic matrix do not form a covalent bond, but are connected by hydrogen bonds and van der Waals forces, they maintain their independent chemical properties, so when they come into contact with water and oil contained in packaged food will dissolve. After research, it was found that phthalate is an environmental hormone substance, which interferes with the production, release, transfer, metabolism, reaction and elimination of normal hormones that mainta...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 胥传来徐丽广马伟李灼坤
Owner JIANGNAN UNIV
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