Influenza hemagglutination inhibition test detection method
A hemagglutination inhibition test and detection method technology, which is applied in the field of influenza hemagglutination inhibition test detection, can solve the problems of rare human red blood cells, shortage of experimental resources, and large amount of experiments
Pending Publication Date: 2020-06-23
广州鸿泉生物科技有限公司
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Problems solved by technology
However, with the increase of the population, the amount of experiments is large, and human red blood cells are scarce, resulting in a shortage of experimental resources.
Method used
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Embodiment 1
[0078] This embodiment provides a method for detecting influenza hemagglutination inhibition test, comprising the following steps:
[0079] S1. Pretreat the standard reference antiserum to remove non-specific inhibins and non-specific lectins in the serum;
[0080] S2. Prepare red blood cell suspension;
[0081] S3. Determination of influenza strain hemagglutination titer;
[0082] S4. Prepare 4 hemagglutination unit antigens for erythrocyte agglutination inhibition test;
[0083] S5. Check and titrate the 4 hemagglutination unit antigens;
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The invention belongs to the technical field of microbiological detection, and discloses an influenza hemagglutination inhibition test detection method which comprises the following steps: pretreatingstandard reference antiserum to remove non-specific inhibin and non-specific lectin in the serum; preparing an erythrocyte suspension; determining the hemagglutination titer of the influenza virus strain; preparing four hemagglutination unit antigens for an erythrocyte agglutination inhibition test; rechecking and titrating four hemagglutination unit antigens; and performing influenza virus identification and antigen analysis by using an HAI method to obtain the serum titer of the detected serum. According to the detection method, chicken erythrocyte, turkey erythrocyte and guinea pig erythrocyte are used as raw materials to prepare erythrocyte suspension, animal erythrocyte is used for replacing human erythrocyte, and the difficulty that an experiment cannot be conducted due to lack of erythrocyte is solved.
Description
technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a detection method for influenza hemagglutination inhibition test. Background technique [0002] The hemagglutinin (HA) protein on the surface of influenza virus contains a structure that recognizes and binds to receptors on the surface of host cells, and can cause some specific red blood cells to agglutinate. When erythrocytes and virus are mixed in an appropriate proportion, the erythrocytes agglutinate due to the action of the virus, which is called agglutination. Antiserum containing specific anti-influenza virus HA protein can inhibit the occurrence of hemagglutination, that is, after the antibody binds to the virus, hemagglutinin can not be adsorbed on the receptor on the surface of red blood cells, which is called hemagglutination inhibition (HAI) . [0003] Trace hemagglutination inhibition test is currently the most commonly used test method...
Claims
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Login to View More IPC IPC(8): G01N33/569G01N33/68C12N5/078
CPCG01N33/56983G01N33/68C12N5/0641
Inventor 杨夏黄庆丽
Owner 广州鸿泉生物科技有限公司
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