A method of an avian infectious laryngotracheitis live vaccine using a cell line includes: replacing chick embryos with the cell line to proliferate avian infectious laryngotracheitis virus liquid, and performing cell line culturing, cloning and purifying, passage, culturing, cloning and culturing of a viral strain, preparing of vaccines, collecting of vaccine preparation virus liquid, subpackaging, freeze-drying and the like. The avian infectious laryngotracheitis live vaccine is produced using passage cells, the production cost is lowered, vaccine immunity side effect is reduced, inter-batch difference is diminished, quality stability of the vaccine is guaranteed, and basis is laid for the large-scale culturing of the virus. Experiments show that bright, round, and vividly outlined cells free of intra-cell impurity can be obtained by only three times of cloning, while an existing method needs seven times of cloning, the cloning time of the present method is shortened, and high activity and high cloning rate are given to cloning.