Construction method of recombinant adenovirus with capacity of fusion expression of gene TM-1 and gene gD, recombinant adenovirus and application

A recombinant adenovirus and fusion expression technology, which is applied in the field of recombinant adenovirus construction, can solve the problems of poor inactivated vaccine effect, mutated new strains, easy to return to ancestors, etc., and achieves the effects of high conservation and low mutation rate.

Inactive Publication Date: 2018-01-19
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

The inactivated vaccine of Mycoplasma gallisepticum cannot prevent the attack of new strains, and the attenuated vaccine is easy to become stronger, and it is easy to produce new mutant strains; the live attenuated vaccine of chicken infectious laryngotracheitis is easy to return to the ancestors, and the inactivated vaccine is less effective

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  • Construction method of recombinant adenovirus with capacity of fusion expression of gene TM-1 and gene gD, recombinant adenovirus and application
  • Construction method of recombinant adenovirus with capacity of fusion expression of gene TM-1 and gene gD, recombinant adenovirus and application
  • Construction method of recombinant adenovirus with capacity of fusion expression of gene TM-1 and gene gD, recombinant adenovirus and application

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Embodiment Construction

[0029] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0030] With the development of molecular biology, immunology, pathology and related disciplines, gene therapy has become a hotspot in the research of disease prevention and treatment. At present, adenovirus (adenovirus, Ad) vector therapy is one of the most promising gene transfer methods in gene therapy. It can effectively deliver foreign genes to various target cells or tissues. The construction and operation of the vector are simple, It has a wide range of hosts and strong infectivity. It can enter different tissues through different channels, and can also infect differentiated non-dividing cells.

[0031] Chicken Chronic Respiratory Disease (CRD) is a chronic, contagious disease of chickens caused by Mycoplasma Gallisepticum (MG), mainly characterized by respiratory clinical symptoms. The main clinical symptoms of the disease are cough, run...

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Abstract

The invention discloses a construction method of a recombinant adenovirus with capacity of fusion expression of a gene TM-1 and a gene gD, the recombinant adenovirus and an application. The gene TM-1of mycoplasma gallisepticum and the gene gD of infectious laryngotracheitis are cloned into a shuttle vector pDC315-EGFP, cells HEK293 are transfected with the shuttle vector constructed successfully,an adenovirus backbone vector pBHGlox (delta)E1 and 3cre simultaneously, so that the shuttle vector and the adenovirus backbone vector are homologously recombined in the 293 cell line by a Cre/loxP recombinase system, the recombinant adenovirus pBH-TM-1-gD containing the target genes is obtained by packaging, and the recombinant adenovirus with high titer is obtained after amplification. The combined effect and relevant mechanism of the gene TM-1 and the gene gD on the two contagious respiratory diseases, namely, the mycoplasma gallisepticum infection and the avian infectious laryngotracheitis virus under the mediation of the adenovirus are evaluated.

Description

technical field [0001] The present invention relates to a method for constructing a recombinant adenovirus, specifically, a method for constructing a recombinant adenovirus for fusion expression of TM-1 gene and gD gene, and the recombinant adenovirus and its application. Background technique [0002] With the rapid development of scale and intensification of the breeding industry, chicken chronic respiratory disease and chicken infectious laryngotracheitis have become one of the important respiratory infectious diseases that seriously threaten the chicken industry. Mycoplasma gallisepticum infection causes chronic respiratory infectious diseases in chickens. Once infected, it is difficult to clear up, and it is easy to develop secondary diseases such as chicken infectious laryngotracheitis, chicken infectious bronchitis, Escherichia coli and other diseases; chicken infectious laryngotracheitis virus infection caused Chicken acute respiratory disease, no specific medicine. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/861A61K39/295A61K39/02A61K39/245A61P31/04A61P31/22
Inventor 金天明李小慧张东超高珂珂林静李昕孟佳丽尚翠玲赵瑞利
Owner TIANJIN AGRICULTURE COLLEGE
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