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I-colony fowl adenovirus 4 strain and application thereof

An avian adenovirus and virus technology, applied in the directions of viruses, antiviral agents, virus antigen components, etc., can solve the problems such as loopholes in the prevention and control of group I avian adenoviruses, and achieve the prevention of chicken hydropericardium hepatitis syndrome and good specificity. , the effect of good immunogenicity

Active Publication Date: 2016-03-02
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the prevention and control of this disease, there are commercial vaccines against group I adenovirus in foreign countries, but there is no vaccine available in China so far, which leads to loopholes in the prevention and control of group I avian adenovirus

Method used

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  • I-colony fowl adenovirus 4 strain and application thereof
  • I-colony fowl adenovirus 4 strain and application thereof
  • I-colony fowl adenovirus 4 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Isolation and Identification of Group I Group 4 Avian Adenovirus YBAV-4 Strain

[0018] 1. Epidemiological survey Since 2010, some broiler breeders, laying hens and Ma chickens in Shandong, Jiangsu and other regions have experienced a disease characterized by high mortality and anatomical manifestations of hepatomegaly and hydropericardium. The disease was initially diagnosed as hydropericardium hepatitis syndrome caused by group I group C-4 avian adenovirus through clinical investigation and laboratory testing. In 2010, the inventor successfully isolated a virus strain from the liver of a dead chicken with typical symptoms of inclusion body hepatitis and hydropericardium in a farm in Zibo, Shandong.

[0019] 2. Virus isolation Take the liver of sick and dead chicken and grind it, add sterilized saline at a ratio of 1:5 to make a suspension; after repeated freezing and thawing 3 times, centrifuge at 3000r / min for 30min, take the supernatant; add penicillin an...

Embodiment 2

[0040] Example 2 Virulence determination of group I group 4 avian adenovirus YBAV-4 strain

[0041] 1 The effect of cytopathic effect (CPE) will reduce the virus content to not less than 10 5.0 TCID 50 Each generation of viruses per 0.1ml was inoculated into well-growing chicken hepatocytes in an amount of 0.5%, and cultured at 37° C. for 36 to 48 hours, and the cell pathological changes were observed. The results showed specific lesions such as cell shrinkage.

[0042] 2 Determination of virus content The above-mentioned viruses of each generation were diluted serially by 10 times with cell maintenance solution, and 10 －6 、10 －7 、10 －8 3 dilutions were respectively inoculated on well-grown chicken hepatocytes (96-well cell plate), each dilution was inoculated into 6 wells, 0.1 ml per well. Set virus positive control wells and cell negative control wells at the same time, at 37°C, 5% CO 2 Cultivate and observe in the incubator for 168 hours, if CPE occurs, it is judged a...

Embodiment 3

[0046] Example 3 Immunogenicity Evaluation of Group Ⅰ Type 4 Avian Adenovirus YBAV-4 Strain

[0047] The C4, C10, C15, and C18 generations of viruses were inoculated with chicken liver cells for reproduction, and the virus content was ≥ 10 7.0 TCID 50 / 0.1ml of virus liquid, after inactivation, make an inactivated vaccine with a ratio of 1:2 (water phase: oil phase), take 20 SPF chickens aged 21 to 28 days, and inject subcutaneously or intramuscularly into each neck of 10 chickens. Live vaccine, 0.3ml each, and the other 10 were not immunized as a control. 21-28 days after inoculation, all immunized chickens and control chickens were intramuscularly injected with group I type 4 avian adenovirus YBAV-4 strain virus liquid (containing about 10 5.5 TCID 50 / 0.1ml), each 0.2ml. Observe for 14 days and record the incidence. Results: The chickens in the immunized group were observed for 14 days, and the protection rate was 9 / 10-10 / 10; the incidence rate of chickens in the contr...

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Abstract

The invention aims at providing an I-colony fowl adenovirus 4 strain, which is preserved with preservation number of CCTCC No. V201541. The I-colony fowl adenovirus 4 YBAV-4 strain disclosed by the invention is excellent in specificity and immunogenicity; a specific precipitation line does not appear in specific positive serum chicken SPF chicken serum such as infected cell sap and egg drop syndrome resisting virus, chicken infectious bursal disease virus, Newcastle disease virus, chicken infectious laryngotracheitis virus, chicken Marek's disease virus, avian influenza and the like, while an obvious specific precipitation line appears in I-colony fowl adenovirus 4 specific serum. The strain disclosed by the invention, as a vaccine strain which is good in manufacturing effect, is capable of preventing chicken hydropericardium syndrome, and the strain is applicable to identification of virus serotype and investigation on epidemiology.

Description

technical field [0001] The invention belongs to the technical field of microbial virus screening, and in particular relates to a group I type 4 poultry adenovirus strain and application thereof. technical background [0002] Group I avian adenoviruses make up the genus Avian Adenovirus in the family Adenoviridae. Group II (Turkey Hemorrhagic Enteritis and Related Viruses) and Group III (Egg Drop Syndrome) adenoviruses are clearly associated with disease, in contrast to most Group I avian adenoviruses that have not been shown to be pathogenic in poultry. totally sure. But FAdV-1 and FAdV-4 are obviously exceptions, among which FAdV-1 can cause quail bronchitis, and FAdV-4 is the main cause of hydropericardium hepatitis syndrome. Other adenovirus strains allow rapid infection of opportunistic pathogens when chicken health is compromised, such as co-infection with other pathogens such as Chicken Infectious Anemia Virus (CIAV) and Infectious Bursal Disease Virus (IBDV) . Gro...

Claims

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Application Information

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IPC IPC(8): C12N7/00G01N33/569A61K39/235A61P31/20C12R1/93
CPCA61K39/12A61K2039/54A61K2039/545C12N7/00C12N2710/10034G01N33/56983G01N2333/075
Inventor 王红申茂欣张恒韩建文李陆梅刘红祥党启峰范根成杜元钊
Owner YEBIO BIOENG OF QINGDAO
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