7 results about "Cell plate" patented technology

The invention provides an organoid membrane protein and intracellular protein immunofluorescence in-situ co-staining method, which comprises the following steps of: sealing and fixing organoid glue drops, adding a mixed solution of membrane protein primary antibody and a Dune phosphate buffer solution containing bovine serum albumin for incubation, performing membrane permeation by using a permeation agent, sealing and washing, and drying to obtain the organoid membrane protein and intracellular protein immunofluorescence in-situ co-staining product. Adding a mixed solution of an intracellular protein primary antibody and a Dune phosphate buffer solution containing bovine serum albumin into the cell plate where the transparent organ-like glue drops are located, incubating and washing, then adding a mixed solution of a membrane protein and a fluorescent secondary antibody of the corresponding species of the intracellular protein, incubating and washing in a dark place, and finally obtaining the membrane protein / intracellular protein fluorescent antibody. And finally, adding a mixed solution of DAPI and a Duncheng phosphate buffer solution, and incubating and washing in a dark place again to obtain the membrane protein and intracellular protein immunofluorescence in-situ co-dyed organoid. According to the method, high-quality and high-fidelity membrane protein and intracellular protein in-situ co-dyeing is successfully realized, the integrity of a three-dimensional structure and the high efficiency of an experimental process are taken into consideration, and the method is convenient to operate, high in repeatability and good in universality.

The application relates to a non-uniform alkaline water electrolysis hydrogen production bipolar plate flow field structure and relates to the field of water electrolysis hydrogen production technical equipment, which comprises a main plate, one side of the main plate is provided with a water inlet part, the side, away from the water inlet part, of the main plate is provided with a water outlet part, a plurality of papillae are arranged on the main plate, and the density of the plurality of papillae is reduced along from the center of the main plate to the outer side of the main plate. The application has the advantages that the distribution of the flow field of the alkaline electrolysis cell plate is improved, the shortest path in the middle of the fluid is improved, the fluid is more uniformly dispersed to the periphery, and the electrolysis efficiency is improved.

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.

Surface polarity of a ferroelectric nematic can be configured to generate vectorial control of the orientation of its bulk polarization field. The contact between a surface with in-plane polarity and a ferroelectric nematic liquid crystal generates preferred in-plane orientation of the ferroelectric polarization field at that interface, which can lead to the formation of fluid or glassy monodomains of high polarization without electric field poling. Materials surfaces can be used as a route to making planar-aligned cells with a variety of azimuthal director / polarization structures, including twisted states, in a π-twist cell, obtained with antiparallel, unidirectional buffing on opposing surfaces, we demonstrate three distinct modes of ferroelectric nematic electro-optic response: intrinsic viscosity-limited field induced molecular reorientation, field-induced motion of domain walls separating twisted states of opposite chirality; and propagation of polarization reorientation solitons from the cell plates to the cell center upon field reversal.

The invention discloses a method for separating and culturing chick embryo forebrain neurons. The method comprises the following steps: (1) carrying out coating treatment on a cell plate by using a poly-D-lysine solution; (2) obtaining chick embryo brain tissue fluid; and (3) culturing neurons. By improving and optimizing a neuron preparation method and a reagent, the chick embryo forebrain neuron with the high purity of 98% or above is obtained.

The utility model relates to the technical field of industrial electrolysis, in particular to an electrolytic bath convenient for replacement of a bath plate, which comprises an electrolytic bath, the bath plate, a base, a moving frame, two connecting rods and a replacement mechanism, the bath plate is arranged on the electrolytic bath, and the electrolytic bath is fixedly connected with the base. The movable frame capable of sliding is arranged between the electrolytic tank and the base, the two connecting rods are fixedly connected to the movable frame, and the replacing mechanism is arranged on the two connecting rods; the technical problem that poison gas in the electrolytic bath escapes in the replacement process of a bath plate replacement device on an existing electrolytic bath is solved.