26 results about "Cell plate" patented technology

The present invention provides a preparation method and application of a high-throughput FAMA virus plate, which belongs to the field of biotechnology. By using a cell culture plate to prepare a high-throughput FAMA virus plate, the technical parameters such as the formula of the fixing solution and the blocking solution are optimized. A high-throughput FAMA virus plate prepared from a 96-well cell plate can detect 7 samples at the same time, increasing the detection throughput by 7 times, simplifying the detection process, shortening the detection time from 4 hours to 2.5 hours, and improving the timeliness of the FAMA method for detecting VZV antibodies; the present invention also provides a method for detecting the uniformity of a high-throughput FAMA virus plate, which is conducive to establishing and improving the quality control standards for the production, manufacturing and application of high-throughput FAMA virus plates. The present invention provides a high-throughput FAMA detection platform, which improves the automation level of the preparation and detection of high-throughput FAMA virus plates, and is conducive to the promotion and application of large-scale VZV antibody automated detection.

The invention provides an organoid membrane protein and intracellular protein immunofluorescence in-situ co-staining method, which comprises the following steps of: sealing and fixing organoid glue drops, adding a mixed solution of membrane protein primary antibody and a Dune phosphate buffer solution containing bovine serum albumin for incubation, performing membrane permeation by using a permeation agent, sealing and washing, and drying to obtain the organoid membrane protein and intracellular protein immunofluorescence in-situ co-staining product. Adding a mixed solution of an intracellular protein primary antibody and a Dune phosphate buffer solution containing bovine serum albumin into the cell plate where the transparent organ-like glue drops are located, incubating and washing, then adding a mixed solution of a membrane protein and a fluorescent secondary antibody of the corresponding species of the intracellular protein, incubating and washing in a dark place, and finally obtaining the membrane protein / intracellular protein fluorescent antibody. And finally, adding a mixed solution of DAPI and a Duncheng phosphate buffer solution, and incubating and washing in a dark place again to obtain the membrane protein and intracellular protein immunofluorescence in-situ co-dyed organoid. According to the method, high-quality and high-fidelity membrane protein and intracellular protein in-situ co-dyeing is successfully realized, the integrity of a three-dimensional structure and the high efficiency of an experimental process are taken into consideration, and the method is convenient to operate, high in repeatability and good in universality.

A gut organoid includes a tissue structure that encloses a cavity. A method of using the gut organoid includes providing a test drug to the gut organoid in the presence of a buffer solution so that liquid comprising the test drug enters into the cavity, and collecting and analyzing the liquid in the cavity. A method for producing the gut organoid includes plating cells on a cell culture substrate including a surface on which a cell-adhesive region and a non-cell-adhesive region surrounding the cell-adhesive region are formed, and culturing the cells plated on the cell culture substrate so as to induce differentiation of the cells.

The present invention is directed to obtaining a cell population comprising mesenchymal stem cells that are excellent in proliferative capability and osteoblastic differentiation capability and are useful for the regeneration of periodontal tissues. The present invention is also directed to providing a cell sheet and a method for producing a cell sheet, comprising, as a material, a cell population comprising mesenchymal stem cells that are excellent in proliferative capability and osteoblastic differentiation capability and are useful for the regeneration of periodontal tissues. A cell population having alkaline phosphatase activity before calcification induction of 1 U or more is selected and obtained from a cell population comprising mesenchymal stem cells derived from a dental or periodontal tissue. A cell sheet is obtained by the following steps (a) to (c): (a) obtaining a cell population comprising mesenchymal stem cells and having alkaline phosphatase activity; (b) culturing the cell population in a calcification induction medium, and proliferating the cell population on a plastic substrate; and (c) recovering and washing a sheet-shaped cell population formed on the plastic substrate.

Embodiments herein disclose a method for fabricating high aspect ratio structures. The method includes depositing a predefined quantity of a viscoelastic fluid on a top surface of a bottom cell plate and compressing the viscoelastic fluid deposited on the top surface of the bottom cell plate using a bottom surface of a top cell plate. The viscoelastic fluid is a blend of a solvent and a polymer. At least one of the top cell plate and bottom cell plate comprises a plurality of lands, sealed source holes and / or unsealed source hole for penetration of a low-viscous fluid. Further, the method includes separating the top cell plate and the bottom cell plate to induce out of plane stretching of the high viscous fluid and obtaining a plurality of high aspect ratio structures between the top cell plate and the bottom cell plate due to the penetration of the low-viscous fluid.

The invention relates to a hybridoma high-throughput screening system and method.The hybridoma high-throughput screening system comprises a cell culture area, a cell analysis area and a transfer area, and the cell culture area comprises a first table board unit, a first storage unit, a first liquid working unit, a culture unit and a first transfer unit; the cell analysis area comprises a second table board unit, a second storage unit, a centrifugal unit, a cell analysis unit, a detection unit, a second liquid working unit, a cleaning unit and a second transfer unit, and the transfer area is arranged between the cell culture area and the cell analysis area. The cell plate transferring device is used for acquiring the cell plate transferred by the cell culture area / cell analysis area and transferring the cell plate to the cell analysis area / cell culture area. The intelligent hybridoma high-throughput screening system has the advantages that by means of the highly-integrated intelligent hybridoma high-throughput screening system, the steps needing manual participation are greatly reduced, the processing efficiency can be improved, and processing errors are reduced.

The invention belongs to the field of biomedicine, and specifically discloses a method for screening a cell line resistant to enzalutamide and an application thereof, comprising the following steps: 1) reviving Lncap cells, and plating cells after the Lncap cells grow to a normal state; 2) after plating the cells, replacing the culture medium with an incomplete culture medium, and starving the Lncap cells; 3) after the starvation treatment, replacing the culture medium with a complete culture medium, and adding testosterone and 0.1-1 mg / mL acetylnitrosourea ENU, and treating for 8-24 hours to induce cell mutation; 4) washing the cells twice with a complete culture medium, and replacing the complete culture medium to continue culturing for more than 24 hours; 5) adding a first concentration of enzalutamide and culturing for one week; 6) using a second concentration of enzalutamide with an increased concentration to culture for one week; 7) using a third concentration of enzalutamide with a further increased concentration to culture for two weeks; 8) after the culture is completed, selecting monoclonal cells for culture amplification, and freezing and preserving; 9) identifying drug resistance to obtain a cell line resistant to enzalutamide.

The problem to be solved by the present invention is to provide an immunoisolation device that achieves both a reduction in diffusion distance, which is effective for increasing the permeability of substances such as physiologically active substances and nutrients, and improvement in durability to withstand long-term transplantation. Another object of the present invention is to provide an immune control technique using a cell sheet. The present invention provides an immunoisolation device comprising a sheet-like cell aggregate containing cells and an extracellular matrix, and an immunoisolation layer that covers the cell aggregate.

The application relates to a non-uniform alkaline water electrolysis hydrogen production bipolar plate flow field structure and relates to the field of water electrolysis hydrogen production technical equipment, which comprises a main plate, one side of the main plate is provided with a water inlet part, the side, away from the water inlet part, of the main plate is provided with a water outlet part, a plurality of papillae are arranged on the main plate, and the density of the plurality of papillae is reduced along from the center of the main plate to the outer side of the main plate. The application has the advantages that the distribution of the flow field of the alkaline electrolysis cell plate is improved, the shortest path in the middle of the fluid is improved, the fluid is more uniformly dispersed to the periphery, and the electrolysis efficiency is improved.

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.

The invention belongs to the field of hydrogen production by water electrolysis, and provides a novel interdigital flow channel structure of a proton exchange membrane electrolytic cell polar plate, which comprises a polar plate main body, and an inlet flow channel and an outlet flow channel arranged on the polar plate, the inlet runner comprises an inlet main runner, a plurality of inlet secondary runners and inlet branch runners; the outlet runner comprises an outlet main runner, a plurality of outlet secondary runners and outlet branch runners; the inlet branch flow channels and the outlet branch flow channels are parallel to each other and are sequentially and alternately arranged to form an interdigital structure; the inlet branch flow channels and the outlet branch flow channels are parallel to each other and are alternately and uniformly distributed on the proton exchange membrane electrolytic cell polar plate in sequence to form an interdigital structure. According to the flow channel structure, the distribution uniformity of fluid in the flow channel is improved, and the pressure loss and impedance loss in the electrolysis process are further reduced, so that the overall performance of the electrolytic cell is improved.

A method of making a cell sheet comprising secondary spheroids, including (a) obtaining cardiosphere-derived cells; (b) cultivating the cardiosphere-derived cells for a first period of time in a first media comprising at least one of an ascorbic acid and an analog thereof, to form secondary spheroids; (c) transferring an amount of the spheroids formed in step (b) into a mold; (d) culturing the secondary spheroids for a second period of time in a second media comprising at least one of the ascorbic acid and an analog thereof, wherein the at least one of the ascorbic acid and an analog thereof is present in an amount effective to promote a formation of an extracellular matrix; and (e) culturing a product obtained in step (d) for a third period of time, in the absence of the at least one of the ascorbic acid and an analog thereof.

The invention discloses a diamond cell plate lattice metamaterial based on sea urchin bionic inspiration and an additive manufacturing method thereof. The bone plates are divided into two groups by taking relative positions of face centers of crystal lattices and internal atoms as geometric constraints, the group A is an equilateral triangular plate, and the group B is an integrated composite plate formed by tightly splicing three full-length equal waist-sharing triangular plates according to the waist-sharing edges; and filling A and B into a unit cell frame according to a diamond lattice atomic layout to obtain a complete bone plate structure. And the spurs are arranged along the waistline direction of the group B of triangles and a path pointing to the vertexes of the crystal lattices, and form a continuously coupled support network with the bone lamella. And taking the bone lamella-bone spur collaborative unit as a prototype, sequentially performing mirror symmetry copying around three orthogonal reference surfaces, constructing a three-dimensional periodic array and obtaining a metamaterial structure. Through parameterization cooperation of the cell size, the cell wall thickness and the aperture, the relative density and anisotropy can be continuously regulated and controlled, and the composite material is suitable for application such as lightweight bearing, energy absorption and vibration reduction isolation.

The application relates to the technical field of soil filling loss prevention, and particularly discloses a soil engineering cell and a large-area backfill soil prevention method, which comprises a first cell plate and a second cell plate, a first through hole is formed in the outer wall of the first cell plate, a second through hole is formed in the outer wall of the second cell plate, the outer wall of the first cell plate is in a wavy shape, and the outer wall of the second cell plate is in a wavy shape; the application facilitates the transportation of the cell, the cell can be stretched into a net shape during construction, and after loose materials such as backfill soil are filled, the cell can form a structure body with the characteristics of strong lateral limitation and large rigidity; meanwhile, the positions of first and second measuring plates are manually adjusted by workers, the clamping groove plate and the clamping plate are connected and fixed, the deformation of the soil cell is buffered, the damage of the soil cell during deformation is reduced, and meanwhile, the workers can measure the area size of the foundation by using the first and second sliding plates, so that the functionality of the device is improved.

Polymeric substrates, optionally in sheet form, treated with a thin layer of photoresist are perforated by laser ablation. Following removal of the photoresist the polymer substrates perforated with holes are patterned in stripes by photolithography, which is followed by synthesis of a cell-adhesive organometallic / self-assembled monolayer of phosphonate (SAMP) interface in the exposed regions, providing well-aligned continuous stripes for various levels of perforation. Cells plated on each of these 2-dimensional (2D) perforated surfaces attach to the interface and spread in alignment with pattern fidelity that is as high as that measured on a non-perforated, patterned substrate. A stack of such 2D patterned polymers yields a 3-dimensional (3D) device which facilitates cell growth and viability via the perforations.

A gut organoid includes a tissue structure that encloses a cavity. A method of using the gut organoid includes providing a test drug to the gut organoid in the presence of a buffer solution so that liquid comprising the test drug enters into the cavity, and collecting and analyzing the liquid in the cavity. A method for producing the gut organoid includes plating cells on a cell culture substrate including a surface on which a cell-adhesive region and a non-cell-adhesive region surrounding the cell-adhesive region are formed, and culturing the cells plated on the cell culture substrate so as to induce differentiation of the cells.

The invention discloses a method for detecting the infectivity of oncolytic viruses in urine and swab preserving fluid based on an ELISpot technology, and relates to the technical field of detection of the infectivity of the oncolytic viruses in the urine and swab preserving fluid, and the specific method comprises the following steps: firstly, placing a proper amount of cells in a transparent cell culture plate for overnight culture, then transferring a sample into a cell plate, and carrying out ELISA detection on the cell plate; culturing for a period of time. An indirect immunofluorescence method is established by adopting an immunofluorescence technology and taking a rabbit anti-Vaccinia Virus Polyclonal Antibody polyclonal antibody as a primary antibody and an Alexa FluorR 488 labeled goat anti-rabbit antibody as a secondary antibody, and the virus infectivity is judged according to the quantity of fluorescent cells infected by the virus. The invention fills the technical blank in the field of detecting the infectivity of the oncolytic virus by using the ELISpot technology, is accurate in detection and high in sensitivity, and has important significance for new drug research and development of oncolytic virus drugs.

The present invention aims to provide a simple method for producing a cell sheet, which allows easy handling of the cell sheet produced by the method. The present invention provides a method for producing a cell sheet, which includes: (1) a step of preparing one or more types of mammal-derived cells; (2) a step of seeding the prepared cells on a porous membrane of a first cell culture vessel having the porous membrane on its bottom; and (3) a step of placing the first cell culture vessel in a second cell culture vessel and culturing the cells, and which allows easy handling of the cell sheet produced by the method; and provides a cell sheet produced by the method.

The invention provides a preparation method and application of a high-throughput FAMA virus plate, and belongs to the technical field of biology, the high-throughput FAMA virus plate is prepared by adopting a cell culture plate, technical parameters such as a stationary liquid formula and a confining liquid formula are optimized, one high-throughput FAMA virus plate prepared from a 96-hole cell plate can detect 7 samples at the same time, the detection throughput is improved by 7 times, and the detection time is shortened. Meanwhile, the detection process is simplified, the detection time is shortened from 4 hours to 2.5 hours, and the timeliness of detecting the VZV antibody by the FAMA method is improved; the invention also provides a method for detecting the homogeneity of the high-throughput FAMA virus plate, which is favorable for establishing and perfecting quality control standards of the high-throughput FAMA virus plate in production, manufacturing and application. The invention provides a high-throughput FAMA detection platform, the automation level of preparation and detection of a high-throughput FAMA virus plate is improved, and popularization and application of large-scale VZV antibody automatic detection are facilitated.

The invention relates to an isolated culture and induced differentiation method of grass carp precursor adipocytes, and belongs to the technical field of adipocyte isolated culture, and the method comprises the following steps: S1, taking grass carp adipose tissues under an aseptic condition, and digesting the tissues with I-type collagenase digestive juice for 30-60 min to obtain grass carp precursor adipocytes; s2, inoculating the digested grass carp precursor fat cells into a 24-hole cell plate, putting the 24-hole cell plate into a 28 DEG C cell culture box for culture, and utilizing a grass carp precursor fat cell culture medium for culture until the cells are adhered to the wall. According to the isolated culture and induced differentiation method of the grass carp precursor fat cells, an enzymolysis method for simplifying primary culture of fish precursor tissue cells is combined with a tissue crushing method and a collagen enzymolysis method, and the precursor fat cells are purified by differential centrifugation; by adjusting the formula of the culture medium, the pH stability of the culture medium can be better maintained, and by adjusting the cell osmotic pressure, the phenomenon that a large number of cells die due to internal and external osmotic pressure difference during digestion can be avoided.

Surface polarity of a ferroelectric nematic can be configured to generate vectorial control of the orientation of its bulk polarization field. The contact between a surface with in-plane polarity and a ferroelectric nematic liquid crystal generates preferred in-plane orientation of the ferroelectric polarization field at that interface, which can lead to the formation of fluid or glassy monodomains of high polarization without electric field poling. Materials surfaces can be used as a route to making planar-aligned cells with a variety of azimuthal director / polarization structures, including twisted states, in a π-twist cell, obtained with antiparallel, unidirectional buffing on opposing surfaces, we demonstrate three distinct modes of ferroelectric nematic electro-optic response: intrinsic viscosity-limited field induced molecular reorientation, field-induced motion of domain walls separating twisted states of opposite chirality; and propagation of polarization reorientation solitons from the cell plates to the cell center upon field reversal.

To store a medical sheet in an external cylinder efficiently in transfer equipment having an external cylinder for transferring a medical sheet (cell sheet) used for organ transplantation to a treatment object part of a living body.SOLUTION: A medical jig 94 includes: a jig body 941 that can be attached to or detached from an outer peripheral surface 22A of an external cylinder 22 in transfer equipment 10; a guide part 942 extending in a tip direction from the tip of the jig body 941 and having an internal space 943 into which a first carrier member is taken; and a partition wall part 945 protruding from an inner peripheral surface of the guide part 942 toward the internal space 943. When causing a first support part 26 and the medical jig 94 to be relatively moved so that the first support part 26 of the transfer equipment 10 is moved relatively in a base end direction with respect to the medical jig 94, the guide part 942 and the partition wall part 945 guide the first support part 26 in the internal space 943, and deform the first support part 26 into a curved shape so that the two sides in a width direction of the first support part 26 protrude toward a first support surface 261.SELECTED DRAWING: Figure 6

The invention discloses a method for separating and culturing chick embryo forebrain neurons. The method comprises the following steps: (1) carrying out coating treatment on a cell plate by using a poly-D-lysine solution; (2) obtaining chick embryo brain tissue fluid; and (3) culturing neurons. By improving and optimizing a neuron preparation method and a reagent, the chick embryo forebrain neuron with the high purity of 98% or above is obtained.

The utility model relates to the technical field of industrial electrolysis, in particular to an electrolytic bath convenient for replacement of a bath plate, which comprises an electrolytic bath, the bath plate, a base, a moving frame, two connecting rods and a replacement mechanism, the bath plate is arranged on the electrolytic bath, and the electrolytic bath is fixedly connected with the base. The movable frame capable of sliding is arranged between the electrolytic tank and the base, the two connecting rods are fixedly connected to the movable frame, and the replacing mechanism is arranged on the two connecting rods; the technical problem that poison gas in the electrolytic bath escapes in the replacement process of a bath plate replacement device on an existing electrolytic bath is solved.

The present invention relates to a method for culturing adult hair follicle bulge cells, belonging to the field of biotechnology, and comprising the following steps: S1. Cleaning and processing the hair follicle tissue to obtain the hair follicle tissue containing bulge cells; S2. Immersing the hair follicle tissue containing bulge cells in a mixed solution of Advanced DMEM / F12 medium containing collagenase, dispase, EGF, bFGF, and 1× penicillin-streptomycin for digestion; S3. Chopping the hair follicle tissue containing bulge cells, centrifuging after terminating digestion, adding a cell culture dissociating agent to the precipitated tissue for digestion twice, centrifuging the supernatant after terminating digestion, collecting the precipitate, resuspending and filtering; S4. Inoculating the cells into a six-well cell plate for 3D suspension culture, changing the medium every other day and pipetting the cells. The present invention uses a two-step method to digest the hair follicle bulge cell tissue site, obtaining a larger amount of hair follicle bulge cells and improving the characteristics and stemness of the hair follicle bulge cells.