134 results about "Poly d lysine" patented technology

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

The invention discloses a preparation method and application of a carbon nanomaterial-immunostimulatory sequence compound. The preparation method comprises the steps of (1) modifying a carbon nanomaterial through polylysine to obtain a polylysine-modified carbon nanomaterial; and (2) mixing the polylysine-modified carbon nanomaterial obtained from the step (1) with an immunostimulatory sequence in a water solution, oscillating for 0.5-3 hours at 20-37 DEG C, and centrifuging, collecting and depositing to obtain the carbon nanomaterial-immunostimulatory sequence compound. According to the carbon nanomaterial-immunostimulatory sequence compound, the carbon nanomaterial serves as a carrier for intracellular transport of the immunostimulatory sequence (CpG DNA), so as to remarkably improve the cellular uptake efficiency of the CpG DNA, protect the CpG DNA from being degraded by nuclease and enhance the immunocompetence of an organism for a long time, so the carbon nanomaterial-immunostimulatory sequence compound has a good medical application prospect.

The invention discloses a method for constructing CD3<->CD56<+>NK cell efficient culture system, and mainly provides a culture system for adhering invitro mass amplified CD3<->CD56<+>NK cells which consist of anti-CD137mAb and IL-15 by using Poly-D-Lysine or Poly-L-Lysine as adhesion agent. Compared with the prior other methods, the method obviously has the advantages of simple and reasonable method, easy promotion, less required raw materials, low cost, shorter preparation cycle, high amplification efficiency, high killing efficiency to tumor cells and so on. Experiments prove that the capability of killing A549 of the CD3<->CD56<+>NK cells which are amplified by the method is increased by one time compared with CD3<->CD56<+>NK cells which are amplified by other known cytokines or cytokine combination in the same cell concentration.

The invention belongs to the technical field of inorganic materials and bioanalysis and in particular relates to an aminophenylboronic acid surface-modified nano-carbon material, as well as a preparation method and application thereof. According to the invention, an available nano-carbon material is subjected to carbodiimide reaction on the basis of acid treatment. Firstly, the surface of the nano-carbon material is modified by polylysine and polyethylene glycol/diglycolic acid, then the nano-carbon material reacts with aminophenylboronic acid for surface functional modification of the nano-carbon material. The synthesis method provided by the invention is simple and efficient. The prepared aminophenylboronic acid-functionalized nano-carbon material, as a nano-adsorbent, has a large specific surface area and strong selective specificity, exhibits better dispersibility and stability in aqueous solutions, and can rapidly achieve the effect of specifically separating and concentrating glycopeptide/glycoprotein. The material is significant in use value and application prospects in the glycoprotein proteomics field and the like.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

The invention relates to an active amino-modified substrate for a microarray and a preparation method of the active amino-modified substrate and belongs to the technical field of medical science and biology. The biological chip substrate is characterized in that amino silanization treatment is performed on the surface of a glass substrate, and polylysine coating is performed on the obtained glass substrate with rich amino on the surface to obtain the active amino-modified glass substrate. The active amino-modified substrate has the advantages that due to the fact preprocessing is performed by using concentrated sulfuric acid and hydrogen peroxide according to optimal proportion, the problem that a common substrate has many impurities is solved, polylysine coating is performed after the amino silanization, the dual combined effect of static absorption and chemical coupling is formed, the fixing rate of the substrate to a probe is evidently increased, and the problems that the substrate is low in sensitivity and poor in stability are solved; the substrate is a biological chip probe vector with excellent performance and quality and is widely applicable to biological chip preparation.

The invention discloses a gene vector as well as a preparation method and application thereof. The gene vector comprises metal nanoparticles as a core and a positive polymer connected to the surfaces of the metal nanoparticles, wherein the positive polymer is polylysine. The invention further discloses a preparation method of the gene vector and application of the gene vector to epidermal stem cell transfection. The gene vector is connected with the metal nanoparticles through polylysine and cell-penetrating peptide, so that the transfection efficiency of the gene vector is improved, the influence on cell toxicity is lowered, the low-toxicity characteristic of polylysine is effectively utilized, and the low transfection efficiency characteristic of polylysine is successfully optimized.

The invention discloses a simulated retrovirus of a targeting tumor; the simulated retrovirus adopts fusion polypeptide consisting of poly-D-lysine and RGD peptide as a genomic vector, packs siRNA retrovirus recombinant and expression vector of gene which is related to the tumor and is formed by self-assembling; a preparation method thereof comprises three steps of the preparation of the fusion polypeptide, the preparation of the siRNA retrovirus recombinant and expression vector and the preparation of the simulated retrovirus; the simulated retrovirus of the targeting tumor of the invention can be targeted and positioned into tumor cells, mediates gene transfection effectively, inhibits the generation and propagation of the tumor obviously, and improves the safety and effectiveness of the tumor gene therapy; and the preparation method is simple and convenient, can be used for preparing anti-tumor drugs and has good application prospect.

The invention discloses an Epsilon-polylysine-vitamin E succinate amide compound, and a preparation method and application thereof. The amide compound has the structure shown as in formula (I), and is obtained by a nucleophilic substitution reaction on Epsilon-polylysine and -vitamin E succinate under the catalysis of N-N dilauryl hydroge dicarboximide. The amide compound has high bacteriostatic ability and emulsibility, and solves the problem of low lipid solubility of Epsilon-polylysine in the prior art.

The invention provides a compound antibacterial agent and a preparation method thereof. The compound antibacterial agent contains the following components in parts by weight: 111 parts of water, 0.5-11 parts of soybean extractive solution, 0.5-2.5 parts of glycine, 0.005-3.0 parts of epsilon-poly-lysine, 0.1-5.0 parts of benzalkonium chloride, 0.01-0.1 part of ergosterol, 0.1-1 part of docosanoic acid, and 0.1-6.0 parts of didecyldimethylammonium chloride. The preparation method of the compound antibacterial agent comprises the steps of diluting soybean extractive solution with water, and proportionally adding glycine, epsilon-poly-lysine, benzalkonium chloride and didecyldimethylammonium chloride. The compound antibacterial agent provided by the invention is not harmful to human and livestock, has high sterilizing capacity, can be used at various sterilizing and disinfecting sites, and has the advantages of low cost, wide application range and good sterilizing effect.

The invention relates to a vascularized tissue engineering bone and a preparation method thereof. The vascularized tissue engineering bone is composed of a support composed of poly-L-lysine (PLL) modified coralline hydroxyapatite (CHA) as well as a vascular endothelium dermatoblast patch and a bone formation cell patch which are sequentially wrapped at the inner layer of the support. According to the invention, an exogenous support obtained by virtue of the PLL modified CHA is beneficial to crawling ingrowth of blood vessels, and vascularization is realized finally, so that a precondition is provided for long term survival of cells in the tissue engineering bone, and organism immune rejection and inflammatory response are also reduced; adipose mesenchymal stem cells are selected for preparing a double cell patch, endothelial cells different in sources do not need to be added, cells are rich in resources and easy to separate and acquire, a trauma on a donor is minimal, ethical principles are not violated, and culture requirements are low. The vascularized tissue engineering bone provided by the invention is simple in preparation method and mild in preparation conditions, has good bone formation and vascularization properties, is consistent with in vivo application requirements, and has good application prospects as a novel vascularized tissue engineering bone.

The invention relates to a junctional complex, in particular to a Gd-DTPA-Polylysine-McAb (anti VEGF) juncitonal complex, a preparation method and the application thereof. The Gd-DTPA-Polylysine-McAb (anti VEGF) juncitonal complex (contrast agent) is prepared by the following methods: a, synthesis of DTPA bisanhydride; b, preparation of DTPA-Polylysine; c, preparation of Gd-DTPA-Polylysine; and d, junction of anti VEGF monoclonal antibody and the Gd-DTPA-Polylysine. With the aid of the expansion effect of Polylysine and the specificity of monoclonal antibody, the contrast agent Gd-DTPA-Polylysine-McAb (anti VEGF monoclonal antibody) has the advantages of high immune activity, good targeting ability, high content of Gd, and the like.

The invention relates to a reinforcing type gingiva repairing and oral cavity care composition and an application thereof. Active components of the composition include bioactive mineral powder and polylysine. The composition has the comprehensive effects of treating oral diseases such as tooth sensitivity, gingivitis, dental plaques, gingival atrophy, dental ulcer, oral cavity dysbacteriosis and the like. The composition has synergistic effects and is especially suitable for people requiring implant post-operation gingival care and daily use, gingival repairing and care after tooth extraction, post-operation daily oral cavity care of cardiac valve transplanting, and people being sensitive to oral bacteria and being liable to be affected by various inflammations and viruses. The composition also has the effect of preventing tooth sensitivity and reinforcing teeth and gingiva.

The invention discloses a method for transfecting Jurkat cells. The method comprises the following steps: adding 0.1mg/mL poly-D-lysine into a 24-pore cell culture plate, standing at the temperature of 4 DEG C overnight, sucking out liquid, uncapping and blowing in a super clean bench, and washing with a PBS (Phosphate Buffered Solution); inoculating Jurkat cells into the culture plate, culturing in an incubator, removing supernatant the next day, and washing with the PBS for 3 times to turn the Jurkat cell into a wall-attaching state; transfecting pLL3.7 plasmids according to conventional transfecting steps of wall-attaching cells; observing the expression amount of GFPs (Green Fluorescent Proteins) under a fluorescence microscope 24 hours after transfection in order to determine the transfecting efficiency. By adopting the method, the contact opportunity between a plasmid compound and cells can be increased after the Jurkat cells are subjected to wall-attaching treatment, and the transfecting efficiency is increased effectively (90 percent or more). The cell viability is not influenced by a wall-attaching treatment process, and exogenous plasmids can be transfected for the Jurkat cells safely.

The invention discloses a preparation method of a difunctional liver cancer targeted nanobubble carrying NET-1siRNA. The method comprises the following steps: (1) synthesizing 3 pairs of NET-1siRNA sequences, and biotinylating the sequences; (2) preparing a biotinylated nanobubble; (3) synthesizing biotinylated galactosylated polylysine; and (4) coupling the biotinylated NET-1siRNA, the biotinylated nanobubble and the biotinylated galactosylated polylysine through a biotin-avidin system to obtain the difunctional liver cancer targeted nanobubble carrying NET-1siRNA. The liver cancer targeted nanobubble can be used in clinical ultrasonic diagnosis as a targeted ultrasonic contrast agent; and more importantly, the NET-1siRNA carried by the liver cancer targeted nanobubble can inhibit tumor growth, so the liver cancer targeted nanobubble can be used in gene therapy of the liver cancer.

The invention discloses gargle. The gargle comprises 0.5-1.2 parts of paeonol, 0.6-1.0 part of eucalyptus oil, 0.8-1.5 parts of eugenol, 0.05-0.1 part of borax, 0.1-0.5 part of epsilon-polylysine, 0.04-0.09 part of borneolum syntheticum, 0.05-0.1 part of menthol, 0.05-0.08 part of natamycin, 20-27 parts of propylene glycol, 15-21 parts of hydrogenated castor oil [PEG-40 (polyethylene glycol-40)], 0.02-0.04 part of citric acid, 1.0-1.9 parts of sweetening agents and 45-62 parts of deionized water. The gargle has the advantage that strong effects of inhibiting pyogenic bacteria in oral cavities still can be realized by the gargle without chemical components such as alcohol, cetylpyridinium chloride, chlorhexidine and triclosan or hormone such as metronidazole and tinidazole under the condition of low usage of the borax.

The invention discloses a magnetic signal probe of a magnetic relaxation time immunosensor. The probe comprises a Gd < 3 + > chelate, polylysine and a superparamagnetic nano-particle of an antibody which are coupled. By coupling polylysine and an antibody to the surfaces of superparamagnetic nano-particles,the antibody-superparamagnetic nanoparticle-polylysine conjugate with the multi-dimensionalspace reticular dendritic structure is obtained; then, succinimide-tetraazacyclododecane tetraacetic acid with chelating performance is coupled to the surface of polylysine, and paramagnetic Gd < 3 +> ions are added; gd < 3 + > is captured through DOTA, and finally, a large number of Gd < 3 + > ions are chelated on the surfaces of the superparamagnetic nano-particles, so that the magnetic relaxation time immunosensing magnetic signal probe with multiple signal amplification is obtained. According to the invention, magnetic separation and magnetic sensing integrated nano magnetic particles andparamagnetic Gd < 3 + > ions are controllably assembled into the multifunctional nano magnetic probe for the first time, so that multiple amplification of sensing signals is realized, and the sensitivity and detection speed of the sensor are greatly improved.

The invention relates to a method for coating graphene on a tip of an atomic force microscope probe. The method comprises steps that the atomic force microscope probe is taken and is immersed into thepolylysine aqueous solution, the atomic force microscope probe is then immersed into the graphene solution after taking out from the polylysine aqueous solution, and the atomic force microscope probeis taken out and is dried. The method is advantaged in that interaction of polycations of the polylysine with anions on the graphene is utilized, the force between the atomic force microscope tip andthe graphene is enhanced, the yield of the graphene-coated AFM probe tip prepared by the impregnation method is greatly improved, the yield can reach over 90%,raw materials are easy to get, operationis simple, and the method has great practical and popularization values.

The invention relates to an active aldehyde group modified substrate applied to a microarray and a preparation method of the active aldehyde group modified substrate, and belongs to the technical field of medicine and biology. A biological chip substrate is a substrate which is characterized in that an amination surface is connected with an aldehyde group. According to the aldehyde group substrate provided by the invention, the substrate is treated by concentrated sulfuric acid and a plasma washing machine, so that the problem of multiple impurity points of the common substrate can be solved; polylysine is firstly prepared into the amination surface, then glutaraldehyde is used as an aldehyde group donor; through combined action of electrostatic adhesion and chemical coupling, the fixing force of nucleic acid is improved; each probe point is full in shape; the stability of the substrate is improved; therefore, the aldehyde group substrate is an excellent biological chip probe carrier and can be widely applied to the preparation of biological chips.

The invention relates to anticorrosion system of infant wet tissue immersion liquid and a preparation method thereof. The anticorrosion system is prepared by mixing 0.5 to 1 part of paeonol, 15 to 20 parts of propanediol, 0.4 to 1 part of eucalyptus oil, 0.8 to 1.5 parts of eugenol, 0.01 to 0.02 part of tea tree oil, 10 to 15 parts of hydrogenated castor oil (PEG-40), 1 to 2.5 parts of sodium hexametahposphate, 0.01 to 0.05 part of Epsilon-polylysine, 0.02 to 0.05 part of natamycin and 60 to 72 parts of deionized water. Chemical bactericides, chemical fungistats and chemical bacterial inhibitors are not used; the safety is high; and sterilization, fungus inhibition and bacterium inhibition properties are good.

The invention provides a preparation method of a tumor organoid. The preparation method comprises the following steps: acquiring a tumor tissue sample; spreading a layer of methacrylated gelatin on acell culture plate to solidify the gelatin and spreading a layer of pigskin gelatin or polylysine gel to solidify the gelatin; placing the sample tissue into a sterile container and chopping the sample tissue in a cell culture medium to obtain a tissue block mass; passing a chopped sample tissue-culture medium suspension through a sterile filter screen; flushing the tissue block mass intercepted by the filter screen to a new sterile container by using the cell culture medium, adding serum from the tumor tissue sample, and performing uniform mixing; adding an obtained mixture into wells of thecell culture plate and performing culture overnight; and observing the tissue block mass and obtaining a tumor organoid when the block mass is adsorbed to an upper layer of a gelatin surface in a semi-inlaying manner and does not float back and forth along with liquid. The method has short time for preparing the tumor organoid, cells in the organoid are well connected and not prone to scattering,whether the organoid has activity or not can be conveniently seen, and the method can also be used for detecting a cell-killing ability.

The invention relates to an NK cell serum-free culture solution and an NK cell culture method. The serum-free culture solution comprises a basic culture solution, a growth-promoting component, an enzyme inhibitor, trace elements and a micro-support body, bovine serum albumin is not contained in the culture solution, after NK cells are cultured, the purification process is simple, and human immuneresponse caused by animal protein is avoided; meanwhile, a micro-support body is arranged in a serum-free culture solution, laminine and polylysine for promoting adherent growth are arranged, and theamplification speed of the NK cells can be increased; trace element zinc is added into the culture medium, so that the antibody expression quantity can be increased, and apoptosis of cells can be inhibited; and copper can also improve the antibody expression quantity. The culture solution provided by the invention can realize efficient culture of the NK cells, and can improve the tumor killing capability of the NK cells.

The invention discloses a storage method of adipose-derived stem cells. The storage method comprises the following steps: separating adipose-derived stem cells from adipose tissues and culturing the adipose-derived stem cells; adding the adipose-derived stem cells into a cell cryopreservation tube filled with an adipose-derived stem cell storage solution, and sealing a tube opening; and freezing the sealed cell cryopreservation tube, wherein every 100mL of the adipose-derived stem cell storage liquid is prepared from the following components: 0.6-4g of carboxylated poly-L-lysine, 1-10g of trehalose, 1-3mL of DMSO, 6-10mL of glycerin, 20-30mL of a serum-free medium, 0.2-2mL of low-molecular dextran, 2-5g of glucose, 80-100U/mL of penicillin, 75-100 [mu]g/mL of streptomycin, and the balanceof a buffer solution. According to the storage method of adipose-derived stem cells, the survival rate of the resuscitated adipose-derived stem cells can be remarkably increased.