14 results about "Poly d lysine" patented technology

The invention relates to the technical field of slide processing. The invention discloses a slide treatment method for improving wall adhesion of 293T cells. The slide processing method comprises the following steps: preparing a cleaning solution; putting the slide into the cleaning solution, soaking, taking out the slide, washing the slide with deionized water, and putting the slide into a drying oven for drying; immersing the dried slide into PLL treatment liquid for 3-7 minutes, taking out and airing; wherein the cleaning solution is prepared from the following raw materials: potassium dichromate, water and concentrated sulfuric acid; the PLL treating fluid is poly-L-lysine with the mass concentration of 0.05%-0.15%, and the solvent is deionized water. The invention further discloses the slide prepared by the slide treatment method for improving the wall adhesion of the 293T cells. According to the slide treatment method, the adhesive force of the slide to 293T cells can be effectively improved, and the cell adherence is enhanced.

The application discloses a kind of composite drug-loaded nanoparticles based on calcium peroxide carrier, which is formed by the surface of hyaluronic acid modified calcium peroxide nanoparticles loaded with polylysine-dihydrophenophor e6 (Ce6) as carrier. pll The composite drug-loaded nanoparticles CaO2@HA / Ce6 are spherical particles with a particle size of about 100nm. pll In the application, Ce6 is converted into Ce6 by polylysine modification, and then combined with hyaluronic acid modified calcium peroxide nanoparticles. pll Ce6 is uniformly loaded on the surface of the composite drug-loaded nanoparticles CaO2@HA / Ce6. pll The application solves the problem that Ce6 cannot be attached to the surface of CaO2 nanoparticles. pll The prepared nanocomposite particles have uniform particle size and high specific surface area. pll After the combination of Ce6 and CaO2@HA, as a good bacterial microenvironment response material, the anoxic condition of bacterial site can be improved, and the photodynamic therapy can be enhanced. On the other hand, the biocompatibility and delivery efficiency of Ce6 are improved, so that significant bactericidal effect can be achieved at low dose.

The invention discloses application of desoxyribonucleic acid-poly-L-lysine composite hydrogel in redistribution of endogenous exosomes and treatment of myocardial infarction, and the desoxyribonucleic acid-poly-L-lysine composite hydrogel comprises a DNA (deoxyribonucleic acid) skeleton and poly-L-lysine wrapping the DNA skeleton, the DNA skeleton contains a nucleic acid aptamer targeting exosome surface protein, and the desoxyribonucleic acid-poly-L-lysine composite hydrogel is used for capturing and releasing endogenous exosomes. According to the invention, the injectable desoxyribonucleic acid-poly-L-lysine composite hydrogel is used for treating myocardial infarction, and targeted enrichment and controllable release of endogenous exosomes are realized through the unique molecular design of the desoxyribonucleic acid-poly-L-lysine composite hydrogel; important breakthrough is achieved in the aspects of targeting, controllability, safety, clinical transformation and the like, and a brand new solution is provided for myocardial infarction treatment.

The invention belongs to the technical field of medical immunodetection analysis, and discloses a kit for detecting an anti-AQP4 autoantibody and application of the kit. The kit comprises a cell pore plate and a cell freezing medium; reaction holes of the cell pore plate are internally coated with a coating substance, and the coating substance is one of polylysine, gelatin and collagen; the cell cryopreservation solution is a cryopreservation protection solution of cells containing overexpressed AQP4 antigens; during coating, the concentration of a coating substance is 0.5 mg / mL-3. 0 mg / mL, and the dosage of the coating substance is 40 [mu] L / hole to 60 [mu] L / hole. According to the kit for detecting the anti-AQP4 autoantibody, the detection performance can be improved, and the kit can be frozen at low temperature for a long time and then unfrozen for detection.

The invention discloses a method for inducing HL-60 cells to generate a neutrophil extracellular trap net and application of the method. The method comprises the following steps: firstly, inducing and differentiating HL-60 cells into neutrophile granulocyte-like cells by using dimethyl sulfoxide (DMSO), and then stimulating the differentiated cells by using phorbol ester (PMA), thereby inducing to form the neutrophile granulocyte extracellular trapping net. Preferably, the final concentration of the DMSO is 0.8%-1.5%, the final concentration of the PMA is 50-400 nM, and the stimulation time is 2-4 hours. According to the invention, a polylysine-coated substrate is adopted to enhance the structural stability of NETs, and SYTOX Green nucleic acid dye is adopted for fluorescence detection. According to the invention, a complete and standardized experimental system formed from the HL-60 cell to the NETs is successfully established, the technical problems of unstable primary cell source and poor experimental repeatability are overcome, the operation is simple and convenient, the result is reliable, and an ideal tool is provided for related research of the NETs.

The application belongs to the technical field of biomedical materials, and particularly relates to a biological adhesive with stable performance and a preparation method and application thereof. Specifically, by regulating a mixing mode of active ester and amino cross-linking agent, using an acidic polylysine hydrochloride aqueous solution as the amino cross-linking agent, directly dissolving the active ester in the polylysine hydrochloride aqueous solution, the solution mixing system formed has stable performance, can be stored at room temperature for 6 hours, an operator can prepare before operation, and can be used at any time during operation, and has good batch stability, avoids individual differences, and therefore has important application value and market prospect.

The application discloses a preparation method of a composite material, which comprises the following steps: S1, obtaining mesoporous silica, adding dopamine, and allowing the dopamine to self-polymerize on the surface of the mesoporous silica; S2, adding a silver source into the reaction system of S1, and carrying out chelation reaction with the polydopamine; S3, adding polylysine into the reaction system of S2; and S4, adding mannose into the reaction system of S3, carrying out coupling reaction with the polydopamine-polylysine, and forming a silver nanoparticle loaded mannose coupled polydopamine-polylysine modified mesoporous silica material. The loading of the polylysine can improve the in-vivo bioavailability and has a broad-spectrum antibacterial performance; the introduction of the mannose coupling can be coupled to the micro-nano material as a target recognition molecule, so that the toxicity generated by off-target is reduced. By utilizing the characteristics that mycobacterium tuberculosis surface highly expresses CD206 receptors, i.e., mannose receptors, and based on the bactericidal effect of silver nanoparticles and Schiff bases, an anti-tuberculosis system of the composite material is constructed.

The invention discloses a desoxyribonucleic acid-poly-L-lysine composite hydrogel and a preparation method thereof, the desoxyribonucleic acid-poly-L-lysine composite hydrogel comprises a DNA skeleton and poly-L-lysine wrapping the DNA skeleton, and the DNA skeleton contains a nucleic acid aptamer targeting exosome surface protein. By optimizing a cross-linking strategy (such as physical entanglement or chemical covalent bonds), the pore size and the stability of the gel are regulated and controlled, and the DNA aptamer capable of identifying the exosome surface protein in a targeted manner is introduced, so that efficient and specific exosome separation is expected to be realized, the key problems of low efficiency, poor specificity, insufficient stability and the like in exosome separation in the prior art are solved, and the method is suitable for large-scale industrial production. The method has wide application prospects in the fields of liquid biopsy, targeted therapy and the like, and a revolutionary technical platform is provided for clinical application of the exosome.

The application provides a method for modifying a solid support suitable for adhering cells, comprising: contacting the solid support with a polylysine-protein factor mixture, the polylysine-protein factor mixture comprising polylysine and a protein factor, the protein factor comprising at least one of an extracellular matrix protein and a cell integrin. The method modifies the solid support, allows the solid support to capture a large number of cells, makes the profile of the cells captured by the solid support clear, and improves the cell capture efficiency of the solid support.

The application provides a kind of composite adjuvant for enhancing cellular immunity and its preparation method and application, belong to biological medicine technical field.The adjuvant includes polylysine hydroxymethyl cellulose polymyxin solution and PEG5000-PLA15000-GM-CSF nanoparticle solution;The concentration of the polylysine hydroxymethyl cellulose polymyxin solution is 0.25-4mg / mL, the concentration of the PEG5000-PLA15000-GM-CSF nanoparticle solution is 0.5-2mg / mL;The volume ratio of polylysine hydroxymethyl cellulose polymyxin solution and PEG5000-PLA15000-GM-CSF nanoparticle solution is (2.5-10):1.The composite adjuvant of the application can activate DC cells, induce stronger immune response, and has better antitumor effect when applied to tumor treatment.

This invention discloses a bone cement possessing both antibacterial properties and high mechanical strength. The invention involves the methacrylylation modification of polylysine, followed by a cross-linking reaction to form a hydrogel, which is then mixed with a solidifying agent powder to successfully prepare a novel bone cement. This material not only exhibits excellent antibacterial activity but also possesses superior mechanical properties and good biocompatibility, making it widely applicable in the field of bone tissue repair and regeneration engineering.

This invention discloses a recombinant human (or bovine) basic fibroblast growth factor gel with wound-healing and antibacterial functions, and its preparation method. Recombinant human (or bovine) basic fibroblast growth factor gel is a genetically engineered topical drug used clinically to promote healing of burn wounds, chronic wounds, and fresh wounds, with significant efficacy. However, its drawbacks include limited antibacterial properties and inability to be used simultaneously with chemical disinfectants such as iodine and ethanol, which, while promoting wound healing, are not conducive to controlling wound infection. Research has found that protamine sulfate or polylysine, with high safety and good antibacterial properties, can exert good antibacterial and anti-infection functions without negatively affecting the bioactivity and stability of recombinant human (or bovine) basic fibroblast growth factor. This represents an advanced method for preparing recombinant human (or bovine) basic fibroblast growth factor gel with wound-healing and antibacterial functions, enabling this type of drug to better exert its clinical effects and expand its application scope.

The application discloses a method for identifying extracellular vesicles, and relates to the technical field of identification of extracellular vesicles. The method comprises the following steps in sequence: dropping a polylysine solution on a glass slide, carrying out coating, air-drying, then adding an extracellular vesicle solution, incubating at room temperature, then adding a fluorescein-5-maleimide solution, incubating at room temperature in the dark, and then carrying out fluorescence identification under a confocal microscope. The method realizes rapid detection of the vesicles by using fluorescein-5-maleimide for marking detection.

The invention discloses a method for separating and culturing chick embryo forebrain neurons. The method comprises the following steps: (1) carrying out coating treatment on a cell plate by using a poly-D-lysine solution; (2) obtaining chick embryo brain tissue fluid; and (3) culturing neurons. By improving and optimizing a neuron preparation method and a reagent, the chick embryo forebrain neuron with the high purity of 98% or above is obtained.