38 results about "Poly lysine" patented technology

Disclosed are water soluble compositions of paclitaxel and docetaxel formed by conjugating the paclitaxel or docetaxel to a water soluble polymer such as poly-glutamic acid, poly-aspartic acid or poly-lysine. Also disclosed are methods of using the compositions for treatment of tumors, auto-immune disorders such as rheumatoid arthritis. Other embodiments include the coating of implantable stents for prevention of restenosis.

Disclosed are water soluble compositions of paclitaxel and docetaxel formed by conjugating the paclitaxel or docetaxel to a water soluble polymer such as poly-glutamic acid, poly-aspartic acid or poly-lysine. Also disclosed are methods of using the compositions for treatment of tumors, auto-immune disorders such as rheumatoid arthritis. Other embodiments include the coating of implantable stents for prevention of restenosis.

A carbohydrate peptide conjugate comprising: a carrier comprising a dendrimeric poly-Lysine enabling multiple epitopes to be covalently attached thereto, at least one peptide comprising one T epitope or several identical or different T epitopes, at least one carbohydrate moiety, or a derivative thereof, containing B epitope, provided it is not a sialoside, or several identical or different epitopes. Use of this conjugate for inducing immune response.

A biological composition intermixed with a polyampholyte protectant for direct implantation has a mixture of biologic material and a volume of polyampholyte protectant. The mixture of biologic material has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components, or whole cells or combinations of the non-whole cellular components and whole cells, wherein the mixture is compatible with biologic function. The volume of polyampholyte protectant is intermixed with the mixture of biologic material, wherein the polyampholyte protectant is a liquid of a polyamine polymer compound of carboxylated poly-lysine and wherein the polyampholyte protectant forms a three-dimensional bonding shroud externally enveloping each of the non-whole cellular components, if any, and each of the whole cells, if any, of the mixture of biologic material.

The invention provides an ovarian cell microcapsule, which adopts alginate-poly-lysine- alginate (APA) to encapsulate ovarian cells and prepare a microcapsule containing ovarian cells inside. The ovarian cells in the microcapsule consists of ovarian granulosa cells and theca cells, which has endocrine function; undifferentiated ovary inner mesenchymal cells and connective tissue cells. If allogenously plant the microcapsule of the invention, the endocrine cells in the microcapsule can constantly secret sex hormone in body, and the endocrine function can be under the control of neuroendorine of the body to reach the aim of personalized supplementing the inefficiency of sex hormone endocrine. The allogenous plant of ovarian cell microcapsule has positive prevention and treatment effects to body degenerative lesion. Experiment shows that the invention can alleviate the loss of bone quantity and presents positive effect to the prevention and treatment of osteoporosis; meanwhile, the invention can affect the biological activity of islet Alpha cell and Beta cell, and regulate the endocrine of insulin and glucagons.

The invention discloses a compound-type nano-vaccine taking an amphiphilic three-block polymer of polyethylene glycol derivative-poly lysine-poly leucine as a nano-carrier bearing a tumor antigen, an immunological adjuvant and siRNA (si Ribonucleic Acid), and a preparation method of the compound-type nano-vaccine. According to the nano-vaccine, taking and presenting of the antigen by antigen-presenting cells can be improved by a nano-micelle bearing the antigen; siRNA is efficiently delivered to TADCs (Tumor-associated Dendritic Cells) to block immunosuppression signals of TADCs, and TADCs are induced and activated by collaboration with the immunological adjuvant, so that a tumor resisting effect of a tumor vaccine is improved; the nano-vaccine has the advantages that the adopted carrier of the nano-vaccine is good in biocompatibility and low in toxicity, and is degradable in a biological body; degradation products are nontoxic and harmless, and can be absorbed or metabolized; and the preparation method of the nano-vaccine is simple and convenient and feasible, good in stability and convenient for popularization.

The invention discloses a cold-resistant foliar fertilizer and a preparation method thereof, the cold-resistant foliar fertilizer includes 10-15 parts of abscisic acid, 10-20 parts of chitosan, 20-30 parts of boric acid, 25-35 parts of potassium carbonate, 5-15 parts of epsilon-Poly lysine, 5-15 parts of humic acid, 10-30 parts of itaconic acid, 1.7-15 parts of trace elements, 0.1-0.5 part of Hexanoic,2-(diethylamino) ethyl estercitrate, 60-80 parts of water and 0.1-1 part of a chelating agent. The preparation method of the cold-resistant foliar fertilizer comprises the following steps: obtaining raw materials according to the components of the cold resistant foliar fertilizer; and stirring and mixing the obtained raw materials at 75-80 DEG C for 25 to 35min. The cold-resistant foliar fertilizer is directly applied to leaf of crops by spraying, the cold-resistant foliar fertilizer contains the most environmentally-sensitive exogenous hormone abscisic acid, and can quickly adjust the crops to change the metaboilic level to adapt to cold damage environment, and by use of various functional components, effects of low temperature on water in plants can be reduced, nutrients and nutritional components for promoting growth and metabolism can be supplemented, and the metabolic ability of the crops in low temperature environment can be maintained stably.

The preparation process of dentritic polylysine derivative includes the stepped repeated condensation reaction of fluorylmethoxylcarbonyl amino acid modified poly(4-hydroxymethylphenoxymethyl styrene) resin as solid phase synthesized medium and N, N-difluorylmethoxylcarbonyl lysine to produce 3G dentritic polylysine with protecting fluorylmethoxylcarbonyl radical; further reaction with carboxy radical containing compound to produce polylysine derivative resin with protecting radical; deprotection with piperidine / methane dichloride; cracking with trifluoroacetic acid to cut down the polylysine derivative from the resin; and precipitation in anhydrous ethyl ether to obtain the one-side branched polylysine derivative. The polylysine derivative thus prepared has high uniqueness and high biocompatibility, and may be used as nucleic acid carrying carrier for gene treating process and may be also used as carrier for antigen and diagnosis contrast.

The invention discloses a diagnose kit for early stage forcast ACS. It is made up form sCD40L standard albumen used as biochemical criterion, single clone antibody and multi-clone antibody including sCD40L, sCD40L single antibody marked by biotin as second antibody. It uses multi sCD40L clone antibody to cover enzyme scale, uses HRP-poly-lysine as signal testing amplification system. The diagnose kit is a super sensitive kit for testing serum sCD40L ELISA, and could improve curative ratio.

Disclosed are water soluble compositions of paclitaxel and docetaxel formed by conjugating the paclitaxel or docetaxel to a water soluble polymer such as polyglutamic acid, poly-aspartic acid or poly-lysine. Also disclosed are methods of using the compositions for treatment of tumors, auto-immune disorders such as rheumatoid arthritis. Other embodiments include the coating of implantable stents for prevention of restenosis.

The invention provides a carboxymethyl cellulose / sodium alginate stem cell micro capsule and a preparation and cultivation method thereof. The preparation method includes the steps that after sodium alginate is dispersed in water, stem cells to be cultured are added, and sodium alginate and stem cell mixed suspension liquid is prepared, carboxymethyl cellulose is dispersed through acetic acid with the volume fraction of 1-2%, and a carboxymethyl cellulose solution is prepared; the mixed suspension liquid is added into the carboxymethyl cellulose solution to be coagulated. Compared with micro capsules cultured through common poly lysine / sodium alginate cells, a membrane coating structure of the micro capsule prepared through the method is of a semi-interpenetrating network structure formed by carboxymethyl cellulose and sodium alginate, good swelling performance, good contracting-expansion performance and other performance are achieved, permeability and stability are better when the micro capsule is used for cell culture, a good cell growing environment can be formed, and cell culture amplification efficiency is improved.

The invention provides a compound antibacterial agent and a preparation method thereof. The compound antibacterial agent contains the following components in parts by weight: 111 parts of water, 0.5-11 parts of soybean extractive solution, 0.5-2.5 parts of glycine, 0.005-3.0 parts of epsilon-poly-lysine, 0.1-5.0 parts of benzalkonium chloride, 0.01-0.1 part of ergosterol, 0.1-1 part of docosanoic acid, and 0.1-6.0 parts of didecyldimethylammonium chloride. The preparation method of the compound antibacterial agent comprises the steps of diluting soybean extractive solution with water, and proportionally adding glycine, epsilon-poly-lysine, benzalkonium chloride and didecyldimethylammonium chloride. The compound antibacterial agent provided by the invention is not harmful to human and livestock, has high sterilizing capacity, can be used at various sterilizing and disinfecting sites, and has the advantages of low cost, wide application range and good sterilizing effect.

A diagnostic test for head and facial pain includes providing a series of antibodies to several biological markers on a test strip, collecting a sample of saliva, and applying the saliva sample to the series of antibodies. If one or more of the anticipated biological markers are present in the sample of saliva, those markers bind to their correlating antibodies thereby providing an indicator of the presence and amount of a particular biological marker in the patient's saliva upon evaluation of the test strip. When applied to a suitable biosensor, the described method can generate an electrical signal that can subsequently be transmitted to other devices. The biosensor includes a substrate that is coated with D-poly lysine on which the various antibodies are randomly arranged. The interaction of biological marker and antibody alters the electrical impedance of the substrate.

The invention relates to a two-step microcapsule preparation method in a bio-artificial reaction device. The invention adopts the technical scheme that biological cell material is fully and evenly mixed in 2 percents of sodium alginate solution, the obtained suspension enters a microcapsule generator from a jet microcapsule generator and then is jetted into calcium chloride solution to react poly-lysine solution and then react with sodium alginate solution, and microcapsules are obtained; and the microcapsules are fully and evenly mixed with sodium alginate solution; the above step is carried out again through the jet microcapsule generator; and microcapsules are obtained after microcapsule cores are liquefied by sodium citrate. The invention overcomes the defect in the prior microcapsules that some cell materials are embedded among capsule membranes of the microcapsules to cause the attack of immune molecules resulting in the damage of the tightness of the microcapsule. The microcapsule produced with the two-step microcapsule preparation method has stronger mechanical tension, still can keep the integrity of the microcapsule walls under the impact of high shear force, and reduces the overflow ratio of cell materials, and the immune insulation function prevents the integrity of the microcapsule walls from being damaged.

This invention discloses a DNA fluorescence capillary biologic sensor (DNA-FCBS) and its preparation method, it belongs to biochemical field. DNA-FCBS is made by using crosslinking agent fixing DNA probe in capillary tube, it includes capillary tube, poly lysine and DNA probe. It can realize the classification of DNA and quantitative determination. DNA sample solution with butt is inhaled by DNA-FCBS for hybridization and coloration reaction, and then the butt DNA is quantitative determined in fluorescence device according to the fluorescence strength. Different butt nucleotides can be determined by changing DNA-FCBS inner probe type, multiple probes are fixed in DNA-FCBS to determine multiple butt nucleotides once time. The DNA-FCBS also can be made into all kinds of DNA fluorescence capillary biologic testing cassette. The reproducibility of this invention is good, operation is simple, and trace quick determination can be realized, so gene detection can be popular and practical.

The invention relates to a compound biological preservative, which is characterized by compounding 300-800mg / L of monosodium fumarate with 200-500mg / L of poly lysine in accordance with the ratio of 1:9-11:1 and then adding 0.02-0.04% of tea polyphenol. The compound biological preservative has the characteristics of high efficiency, safety and no toxic and side effect, is good in broad-spectrum antibacterial property, is low in minimum inhibitory concentration, can not only inhibit the growth of bacterial colony in food, but also can maintain the moisture content, sensory quality and a variety of flavor components, and the compound biological preservative is suitable for the preservation of food, is especially suitable for casual fish sheet preservation, and has the very good application prospect.

The invention discloses an intelligent anticancer polymer drug system. Phosphatidylcholine is subjected to an atom transfer radical polymerization reaction to obtain poly-phosphatidylcholine. Lysine benzyl ester carboxylic acid anhydride is subjected to a ring-opening polymerization reaction to obtain poly lysine benzyl ester. The poly lysine benzyl ester is grafted to doxorubicin through a hydrazone bond. The poly-phosphatidylcholine is bonded to the poly lysine benzyl ester in a manner of click chemistry to obtain a block copolymer. The block copolymer is dissolved in tetrahydrofuran to obtain a solution. The solution is transferred to a dialysis bag for carrying out a dialysis process through pure water to obtain a dialysate which is filtered through a filter membrane. A freeze drying process is carried out to the filtered solution to obtain a drug carrier micelle. The drug carrier micelle has a core-shell double-layer structure with an external layer being hydrophilic poly-phosphatidylcholine and an internal layer being drug molecule wrapping layer. The drug carrier micelle has advantages of belonging to nano particles and enabling a targeting transmission of a drug to cancer cells and a pH-sensitive releasing process in the cancer cells to be achieved. The drug carrier micelle is large in drug carrying amount and good in stability. Targeting function of the drug carrier micelle can effectively reduce toxic and side effects of drugs on normal tissues and organs.

The invention provides a functionalized mesoporous silica tumor targeted transportation controlled-release system and a preparation method thereof. Cyclodextrin is used for coating mesoporous silica,and further coating poly-lysine; amino amidation treatment is performed to obtain a system with high medicine carrying and release controlling. A preparation process comprises preparation of p-toluenesulfonyl-beta-cyclodextrin, preparation of nitriding cyclodextrin, synthesis of S-(2-aminoethylmercapto)-2-mercaptopyridine hydrochloride, preparation of mercapto functionalized mesoporous silica nanoparticles, preparation of disulfide functionalized mesoporous silica nanoparticles, preparation of alkynyl functionalized mesoporous silica nanoparticles, preparation of medicine-carrying mesoporous silica nanoparticles, preparation of amantadine-terminated poly-lysine, wrapping modification of poly-lysine, and amino amination modification. By adopting the preparation method, the targeted enriching and release of medicaments is realized finally, and high medicament utilization rate is achieved.

The present invention discloses dendrobium officinale leaf cakes and a production method thereof, and belongs to the technical field of food processing. The production method conducts a comprehensive utilization of the dendrobium officinale leaves and develops the new products of the dendrobium officinale leaf cakes. The low-sugar and high-fiber dendrobium officinale leaf cakes are prepared by using eggs, glutinous rice, dendrobium officinale leaves, and low-gluten wheat flour as the main raw materials, using white sugar and xylitol as accessory materials, and adding nano-fibers, poly-lysine, etc. The dendrobium officinale leaf cakes can be used as health-care food for middle aged and elderly people. The production method is simple in technology, energy-saving, and easy to operate, and can realize industrial production.

The invention discloses a process for producing ε-polylysine by adsorption and immobilization fermentation. The adsorption and immobilization material is fixed in a fermenter, and the ε-polylysine production strain is inserted to realize the production of ε-polylysine. Adsorption and immobilization of bacterial strains on adsorption and immobilization materials, and production of ε-polylysine by immobilization and fermentation of the production bacterial strains. Utilizing the process for producing ε-polylysine through adsorption and immobilization disclosed in the present invention can effectively improve the problems that mycelium is easy to attach to the wall of the fermenter during the fermentation process of ε-polylysine. In addition, the process can effectively improve the The production efficiency of ε-polylysine is high, and it has the advantages of high product concentration and stable batch production, and is suitable for the industrial production of ε-polylysine.

A carbohydrate peptide conjugate comprising a carrier comprising a dendrimeric poly-Lysine enabling multipic epitopes to be covalently attached thereto, at least one peptide comprising one T epitope or several identical or different T epitopes, at least one carbohydrate moiety, or a derivative thereof, containing B epitope, provided it is not a sialoside, or several identical or different epitopes. Use of this conjugate for inducing immune response.

The invention discloses a preparation method for mosquito-killing potting soil, and belongs to the technical field of the mosquito-killing application. The preparation method comprises the following steps: using urea and multiple types of bacteria and the like as raw materials, preparing a biological bactericide through fermenting, combining with other active ingredients, attracting mosquitoes, and killing the mosquitoes, finally decomposing in-vivo tissues of the mosquitoes as nutrients and killing pathogenic bacteria carried by the mosquitoes, and reducing transmission of diseases; modifyingbentonite, effectively improving antibacterial capability of a system by cooperating with poly-lysine, enabling the mosquitoes to experience a process from excitement, spasm, and paralysis to death,and achieving the effect of killing the mosquitoes, using an octopus salivary gland as a raw material and extracting multiple proteins which have the decomposition effect to a mosquito body, converting the proteins to the simple nutrients, using caustic soda, pig skin grease and the like to prepare a compound mosquito-trap ingredient, and achieving the effect of compound mosquito-trap. The methodis capable of solving problems that current common mosquito-killing liquid is single in effect, and poor in mosquito-killing effect.

A compound for polymeric targeted gene delivery carrier consisting of polyethylene glycol (PEG) grafted poly(L-lysine) (PLL) and a targeting moiety (TM), wherein at least one free amino function of the PLL is substituted with said PEG, at least one free amino function of the PLL is substituted with the TM, and the grafted PLL contains at least 50 % unsubstituted free amino function groups. TM is preferably lactose or galactose which are capable of specifically targeting a hepatoma cell or a liver cell. The new synthetic carriers with various substitution ratios of TM-PEG were characterized using NMR spectroscopy. The new polymeric gene carriers of this invention are capable of forming stable and soluble complexes with nucleic acids, which in turn are able to efficiently transform cells. PEG attached to the PLL gives better solubility properties to the gene / carrier complex and improved transfection efficiency without considerable cytotoxicity. Methods of preparing and using the TM-PEG-PLL as polymeric gene carriers to efficiently transfect cells are disclosed.

The invention discloses a stabilizer for extending the quality guarantee period of acidophilus milk, which comprises the following components in percentage by mass: 25-35% of acetylated starches, 25-35% of phosphorylated starches, 20-30% of R-polysaccharide and 10-20% of poly lysine. Furthermore, the stabilizer comprises the following components in percentage by mass: 30% of acetylated starches, 30% of phosphorylated starches, 25% of R-polysaccharide and 15% poly lysine. The preparation method of the stabilizer comprises the following steps: mixing the components in percentage by mass, and then carrying out instantaneous high-temperature disinfection on the obtained mixture. An acidophilus milk beverage contains the stabilizer for extending the quality guarantee period of acidophilus milk. The stabilizer disclosed by the invention can prevent the separation of whey, improves the taste, improves the product quality, and improves the product stability; the stabilizer can extend the quality guarantee period of acidophilus milk effectively without cold-chain control, and the quality guarantee period of products can reach 6 months at normal temperature; and tastes and structure properties of acidophilus milk can be retained better; and the stabilizer is moderate in cost, and conducive to industrial production.

The invention discloses polylysine for support frame coating, and belongs to the field of medical use. A preparation method comprises the following steps of 1, inoculating strains into a culture medium for culture; performing culture for 40 hours at 25 to 30 DEG C; 2, inoculating the culture liquid into the fermentation culture medium according to the inoculating quantity of 5 percent; performingculture for 48 hours at 25 to 30 DEG C; 3, performing centrifugation on the fermentation liquid for 20 minutes; collecting cells; after the water bath heating, taking liquid supernatant; draining thewater; preparing polylysine; 4, dissolving 2-amino-3-hydroxyphenylpropionic acid monomers and peroxide initiators into an organic solvent to obtain a solution; then, mixing the obtained solution withthe prepared polylysine powder; performing grafting modification on the mixture through melt grafting reaction equipment to obtain polylysine grafting 2-amino-3-hydroxyphenylpropionic acid biodegradable polymers.

The invention discloses a method for culturing luminous pearls with pinctada martensi. The method for culturing the luminous pearls with pinctada martensi includes the following steps of selecting waters, wherein the waters with tropical or subtropical monsoon climates are selected, the waters are high in seawater salinity, large in evaporation capacity, good in water quality and free of pollution, and indexes of cadmium, lead, arsenic, mercury and polychlorinated biphenyl in the waters all accord with national marine quality first-class standards; selecting insertion nuclei, wherein digestionliquid is prepared with trypsin, required small piece shells are dissociated, small piece shells with mantle cell pieces are selected, and the small piece shells had better be white; culturing pearlsacs, wherein luminous pearl nuclei are painted with fibronectin, poly-lysine, rat tail collagen and an SM substance, high-pressure steam sterilization is conducted after drying, cell suspension of mantle tissue is sucked and dropped on the luminous pearl nuclei, mantle tissue blocks are attached to the luminous pearl nuclei, and then a medium is sucked and dropped to conduct culturing for three days. The method for culturing the luminous pearls with pinctada martensi has the advantages of increasing the yield, improving the quality of the pearls and making the pearl industry actually achievehigh yield and high quality.

The present invention discloses an adriamycin-containing macromolecule drug. The preparation method comprises: adopting an atom transfer radical polymerization method to polymerize methacrylic acid to obtain polymethacrylic acid; modifying the polymethacrylic acid with folic acid molecules; adopting a ring-opening polymerization method to polymerize lysine benzyl ester carboxylic anhydride to obtain poly lysine benzyl ester; grafting adriamycin on the poly lysine benzyl ester; bonding the polymethacrylic acid and the poly lysine benzyl ester through a click chemistry method to obtain a block copolymer; respectively dissolving the block polymer in tetrahydrofuran, transferring into a dialysis bag, carrying out dialysis with purified water, and filtering the dialyzed solution with a filtration membrane; and carrying out freeze-drying on the filtered solution to obtain the drug-loaded micelles, wherein the drug carrier micelles have a core-shell double-layer structure, the outer layer is the hydrophilic polymethacrylic acid, and the inner layer is the drug molecule wrapping layer. According to the present invention, the material has the following advantages that: the material belongs to the nanoparticles; the targeted drug delivery on the cancer cells and the pH-sensitive drug release in the cancer cells can be achieved; the drug loading is high; the stability is good; and with the targeting function, the toxic-side effect of the drug on the normal tissues and organs can be effectively reduced.

The invention discloses a chilli yield-increasing fertilizer. The chilli yield-increasing fertilizer comprises the following components in parts by weight: 54 parts of large-leaf sophora japonica, 35 parts of polymaleic anhydride, 25 parts of carbendazim, 74 parts of lard, 35 parts of composite fertilizer, 54 parts of monopotassium phosphate, 55 parts of urea, 23 parts of cordyceps sinensis, 34 parts of potassium molybdate, 31 parts of dry pig manure, 23 parts of bone meal, 36 parts of polylysine, 23 parts of salt and 48 parts of epoxy resin powder. Therefore, the yield-increasing fertilizer dissolves easily-dissolved components firstly and then dissolves insoluble components during use, so the fertilizing times are reduced, and the produced chilli is large in size and sufficient in weight.

The invention relates to a compound biological preservative, which is characterized by compounding 300-800mg / L of monosodium fumarate with 200-500mg / L of poly lysine in accordance with the ratio of 1:9-11:1 and then adding 0.02-0.04% of tea polyphenol. The compound biological preservative has the characteristics of high efficiency, safety and no toxic and side effect, is good in broad-spectrum antibacterial property, is low in minimum inhibitory concentration, can not only inhibit the growth of bacterial colony in food, but also can maintain the moisture content, sensory quality and a variety of flavor components, and the compound biological preservative is suitable for the preservation of food, is especially suitable for casual fish sheet preservation, and has the very good application prospect.