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Carboxymethyl cellulose/sodium alginate stem cell micro capsule and preparation and cultivation method thereof

A technology of carboxymethyl cellulose and sodium alginate, applied in bone/connective tissue cells, animal cells, vertebrate cells, etc., to achieve good permeability and stability and improve efficiency

Inactive Publication Date: 2016-01-20
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of existing sodium alginate / polylysine microcapsules, to provide a kind of carboxymethyl cellulose / sodium alginate stem cell microcapsules and its preparation and cultivation method

Method used

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preparation example Construction

[0017] The example of the present invention proposes a kind of preparation method of carboxymethyl cellulose / sodium alginate stem cell microcapsule, and the steps comprise:

[0018] S10, obtaining carboxymethyl cellulose and sodium alginate;

[0019] S20, after dispersing sodium alginate in water, adding stem cells to be cultured to prepare a mixed suspension of sodium alginate and cells;

[0020] S30, dispersing carboxymethyl cellulose with 1-2% acetic acid to make carboxymethyl cellulose solution;

[0021] S40, adding the mixed suspension of sodium alginate and cells into the carboxymethyl cellulose solution, and obtaining microcapsules after coagulation.

[0022] In the present invention, carboxymethyl cellulose is used as the coating material cross-linked with sodium alginate, and carboxymethyl cellulose is formed after cellulose etherification (carboxymethylation reaction is a kind of etherification technology). Anionic cellulose ether has a high degree of substitution ...

Embodiment 1

[0038] Isolation of rabbit bone marrow mesenchymal stem cells (in this example, rabbit bone marrow mesenchymal stem cells are taken as an example):

[0039] S01, primary culture:

[0040] Take a New Zealand white rabbit weighing about 2kg, and inject 3% barbital-free sodium into the ear vein; after anesthesia, use a 18-gauge bone needle to extract 5ml of bone marrow fluid from the femur, and anticoagulate with 3000U / m1 heparin; Add the bone marrow fluid to LG-DMEM medium for 1:1 dilution, mix well and centrifuge at 1200rpm for 10 minutes; discard the supernatant serum and fat after centrifugation, then add LG-DMEM medium to dilute; then carefully add the suspension to Ficoll cells On the separation liquid, mix according to the volume ratio of 1:1 and then centrifuge at 2500rpm for 30 minutes. After centrifugation, absorb the buffy coat, add LG-DMEM medium to wash twice, centrifuge at 1200rpm for 5 minutes, remove the supernatant, and add LG-DMEM medium containing 10% fetal bo...

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Abstract

The invention provides a carboxymethyl cellulose / sodium alginate stem cell micro capsule and a preparation and cultivation method thereof. The preparation method includes the steps that after sodium alginate is dispersed in water, stem cells to be cultured are added, and sodium alginate and stem cell mixed suspension liquid is prepared, carboxymethyl cellulose is dispersed through acetic acid with the volume fraction of 1-2%, and a carboxymethyl cellulose solution is prepared; the mixed suspension liquid is added into the carboxymethyl cellulose solution to be coagulated. Compared with micro capsules cultured through common poly lysine / sodium alginate cells, a membrane coating structure of the micro capsule prepared through the method is of a semi-interpenetrating network structure formed by carboxymethyl cellulose and sodium alginate, good swelling performance, good contracting-expansion performance and other performance are achieved, permeability and stability are better when the micro capsule is used for cell culture, a good cell growing environment can be formed, and cell culture amplification efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of microencapsulated cell culture, and in particular relates to a carboxymethylcellulose / sodium alginate stem cell microcapsule and a preparation and culture method thereof. Background technique [0002] The microcapsule itself is a composite structure of coatings formed by encapsulating dispersed solid substances, liquid droplets or gases in a dense film. The dense film used for coating is usually made of natural or synthetic polymer materials . Because it has the above-mentioned coated microcapsule structure, in its functional application, biological macromolecules such as enzymes, coenzymes, proteins, or animal and plant cells can be surrounded in bead-shaped microcapsules, while small molecule substances and nutrients in the medium Substances can freely enter and exit the semipermeable membrane to facilitate catalysis or culture, so microcapsules are often used in three-dimensional cell culture, that is...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12N11/10C12N11/04C12N5/0775
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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