36 results about "Cells/microL" patented technology

The invention discloses a step-by-step targeting placenta trophoblast micro-nano robot as well as a preparation method and application thereof. The micro-nano robot comprises a yeast cell wall of a bag-shaped structure, bio-enzyme unevenly distributed on the surface of the yeast cell wall and targeted placenta trophoblast drug-loaded nanoparticles located in the yeast cell wall. The surfaces of the targeted placenta trophoblast drug-loaded nanoparticles are connected with placenta-like chondroitin sulfate A binding polypeptides. Inflammation placenta tissue can be targeted through macrophages, the medicine is accurately delivered to placenta trophoblastic cells through the medicine-carrying nano-particles connected with the targeting peptide, step-by-step accurate targeting is achieved, the medicine does not penetrate through the placenta barrier to reach the body of a fetus, and high biological safety is achieved for pregnancy administration.

The invention belongs to the technical field of biological medicine, and particularly relates to an engineered cell microvesicle and a delivery system based on the engineered cell microvesicle, the system realizes efficient preparation of 1-5 [mu] m cell microvesicles, and the cell microvesicles have a large space volume and can be used for preparing the cell microvesicles. The carrier can be used for loading and delivery of target protein, polypeptide and recombinase which are specifically expressed in mother cells. The system transfects mother cells through lentivirus transfection or plasmid transfection to further produce cell microvesicles, and the microvesicles can load more goods and inherit membrane proteins of the mother cells, and can also effectively load intracellular proteins to realize effective delivery. By virtue of good structural stability, high immunogenicity and excellent biocompatibility, the cell microvesicle reduces systematic toxic and side effects of a traditional carrier, is expected to become an effective drug delivery system, and has great application potential in the field of gene therapy.

The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroidal or spherical shape or substantially ovoid, cylindrical, spheroidal or spherical shape, the smallest dimension of which is between 200 and 400 μm, comprising an external hydrogel layer defining an internal part, said internal part having a granzyme B content of less than 1 μg / ml of medium and comprising between 500 and 5000 lymphocytes forming a three-dimensionally grouped culture. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

The invention discloses a construction method and application of a human lung 3D cell microsphere and a human pulmonary fibrosis 3D organoid. The construction of the human lung 3D cell microspheres comprises the following steps: respectively digesting human primary fibrosis cells and human II-type alveolar epithelial cells which are subjected to adherent culture into single cells; mixing digested human primary fibrotic cells, human II-type alveolar epithelial cells and THP-1 macrophages subjected to suspension culture according to a cell number ratio of 1: 1: 1, so as to obtain a cell precipitate; and preparing the cell precipitate into cell matrigel microspheres by using a T-type microfluidic technology, and culturing to obtain the human lung 3D cell microspheres. And further inducing the human lung 3D cell microspheres by using a human lung organoid culture medium containing 50ng / mL of TGF-beta for 24-48 hours to obtain the human pulmonary fibrosis 3D organoid. According to the invention, 3D cell microspheres with human normal lung cell characteristics and protein expression can be formed within 3 days, and pulmonary fibrosis 3D organs can be obtained after short-time induction.

The invention relates to the technical field of image processing, and further relates to a tumor characteristic spectrum construction method based on CTC enrichment and multi-omics analysis. The method comprises the following steps: 1, sequentially carrying out spiral inertial focusing and deterministic lateral displacement sorting on a whole blood sample to be detected, and finishing single-cell microcavity positioning capture of circulating tumor cells in a single-cell microcavity capture array region; 2, acquiring a time-varying curve of an oxygen molecule concentration value for each positioned and captured circulating tumor cell, and acquiring a metabolic function characteristic parameter group; 3, forming a multi-omics joint feature vector in combination with the metabolic function feature parameter group; and 4, constructing a circulating tumor cell subset based on the multi-omics combined feature vector, extracting symbolic molecular features, and integrating and outputting a tumor feature spectrum. According to the invention, more stable technical support can be provided for tumor heterogeneity analysis, metastasis potential evaluation and curative effect monitoring.

The present invention provides methods of detecting breast cancer or breast cancer cells comprising: (a) obtaining a liquid sample from a subject which sample comprises cells, microvesicles and / or exosomes; and (b) determining the presence of soluble or cell surface associated Cyr61 in the sample; wherein the liquid sample is selected from blood, and / or bone marrow aspirate and wherein the determination of the presence of Cyr61 comprises detection via ELISA.

The application provides a 5mCpG high-throughput detection method TnM-seq based on TnM transposase, which adopts transposase TnM including an MBD domain to detect 5mCpG in situ, and comprises the following steps: after pretreatment of cells to be detected, the cells are incubated with transposase TnM; DNA in the target sample after transposition is recovered and purified; the purified DNA is subjected to PCR library construction; and the library construction product is subjected to high-throughput sequencing. The new transposase TnM newly designed and synthesized is used, combined with high-throughput sequencing, to realize convenient, fast, low-cost and high-sensitivity detection of 5mCpG in a sample. Based on the transposase TnM, a multi-omics technology HAM-seq for simultaneously detecting histone modification H3K9me3, chromatin accessibility and DNA methylation modification 5mCpG in the same sample is further developed, and after being combined with a commercial single-cell microfluidic platform, an epigenome and gene expression four-omics technology EpiX-seq for single-cell analysis in a complex tissue is obtained.

A system and method are provided for enriching cell-free microbial nucleic acids from one or more target samples.

The present application relates to the fields of biology, medicine and bioinformatics, in particular to a method for separating or purifying peripheral red blood cell micronuclei DNA and a use thereof.

The present invention relates to the fields of cell transduction and transfection in a three-dimensional cell culture model and concerns, in particular, a specific cell microcompartment enabling transduction and / or transfection to be carried out within the internal part of said microcompartment.

The use of cellular microparticles in the treatment of respiratory viral pneumonia has been disclosed. Tumor cell-derived microparticles (MPs) include cellular vesicles released by apoptotic tumor cells. The cellular vesicles express spike protein-binding receptors on their surfaces. MPs have high biosafety. In addition, it has been found that the receptors on the surface of microparticles have virus adsorption ability, and macrophages can efficiently take up MPs, which can be used to treat viral pneumonia.

The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroid or spherical shape or of substantially ovoid, cylindrical, spheroid or spherical shape, the smallest dimension of which is between 200 and 400 μm, comprising an outer hydrogel layer that delimits an internal part, said internal part comprising, in a medium, between 500 and 5000 lymphocytes forming a grouped three-dimensional culture and having a TNF alpha content of less than 100 ng / ml of medium. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

The invention relates to cellular microcompartments, each microcompartment successively comprising the following layers, which are organised around at least one lumen: —at least one inner layer of human cells undergoing cardiac differentiation, expressing at least one gene chosen from PDGFRα, MESP-1, NKX2-5, GATA4, MEF2C, TBX20, ISL1 and TBX5, —at least one intermediate layer of isotonic aqueous solution, and—at least one outer hydrogel layer. The invention also relates to the cardiac tissues obtained from said microcompartments and the use thereof, particularly in the treatment of heart disease.

The present invention relates to a clinically usable "GMP" compliant cell microcell comprising at least: a. A cellular layer, b. An outer hydrogel layer, and c. A fibrin web disposed between the outer hydrogel layer and the cellular layer. The invention also relates to a method for producing such a microcompartment and to the use of a kit for producing a cell microcompartment comprising a fibrinogen solution and a thrombin solution.

This invention belongs to the field of cell culture technology, specifically relating to a method for preparing 3D hybrid cell microspheres and their applications. The invention involves co-seeding olfactory mucosal basal stem cells and olfactory mucosal mesenchymal stem cells in a low-absorption culture plate to obtain 3D hybrid cell microspheres. In the 3D hybrid culture system, the two cell types provide support for the state of the olfactory mucosal basal stem cells and olfactory mucosal mesenchymal stem cells, mutually supporting each other and further enhancing their biological characteristics, making them easier to colonize at lesion sites, thereby improving the anti-apoptotic ability of the 3D hybrid cell microspheres. The 3D hybrid cell microspheres prepared by this method offer promising applications for stem cell therapy of respiratory diseases and provide a foundation for using olfactory mucosal-derived stem cells in regenerative medicine.

The application discloses a cell microdroplet identification method based on XGBoost, and relates to the technical field of single-cell RNA sequencing, wherein step one is to search for the boundary of cells and empty droplets by using a RankMSE index and to construct a training set, and to solve the class imbalance problem in the training data by using multiple rounds of downsampling; step two is to combine the gene expression of cells and the cell quality control features calculated in advance such as cell entropy, and to use a machine learning method XGBoost to construct a cell-empty droplet binary classification model suitable for the current data; and step three is to iterate the cell-empty droplet binary classification model, add newly predicted empty droplet data to the model for retraining, and obtain an optimized prediction model. The cell microdroplet identification method based on XGBoost has the advantages that cells can be robustly identified in different data sets, and empty droplets and low-quality cells or cell fragments in the data can be effectively excluded, and the method has higher accuracy and stability compared with the prior art.

The invention provides a scaffold-free 3D hepatocyte micro-tissue as well as a preparation method and application of the scaffold-free 3D hepatocyte micro-tissue. According to the method, through a one-step transplantation strategy, hepatocytes and interstitial cells are mixed and then subjected to 3D co-culture, and the scaffold-free 3D hepatocyte micro-tissue with a tissue structure similar to that of a natural liver is prepared. According to the method, the step of repeated injection in the traditional technology is simplified, the clinical operability is improved, and the prepared 3D hepatocyte micro-tissue can be widely applied to drug screening, toxicity testing, establishment of a liver disease model, liver tissue engineering and cell transplantation and treatment of liver diseases.

Provided are systems and methods for enriching cell-free microbial nucleic acids from one or more subjects' samples.

The present invention relates to the field of three-dimensional cell culture and in particular to cell microcompartments free of extracellular matrix of animal origin and / or derived from cancer cell lines for the production of GMP grade cells and tissues.

The invention relates to the technical field of three-dimensional cell micro-compartment culture. In particular, the present invention relates to a method for preparing a three-dimensional cell microcompartment, the method comprising: carrying out the encapsulation of at least one cell in a hydrogel in order to form a suspension comprising the cell microcompartment in an immersion solution, and at least one step of applying a gravity of at least 10 g to at least one cell and / or at least one cell microcompartment. The invention also relates to a device suitable for carrying out at least part of the method.

The present application relates to the field of oocyte / embryo vitrification, and particularly relates to a method for preparing and vitrification of oocyte / embryo-loaded hydrogel microspheres. The present application first prepares oocyte-loaded cell microspheres encapsulated by sodium alginate hydrogel through a microfluidic chip, then places the collected cell-loaded microspheres in a freezing carrier, then places the freezing carrier loaded with the microspheres in a vitrification solution for CPA loading, and finally puts the freezing carrier loaded with the microspheres into liquid nitrogen for freezing preservation. The microfluidic chip used in the present application can stably generate oocyte-loaded sodium alginate microspheres with high dispersity and uniformity, the microsphere empty package rate and oocyte loss rate are low, and the survival rate and later development of the oocytes are good; the oocyte vitrification preservation technology can effectively reduce the concentration of the protective agent and the loading time, reduce the toxic effect and osmotic damage to the cells, and improve the survival rate and development rate of the oocytes.

The present invention relates to a closed three-dimensional cellular microcompartment or assembly of three-dimensional cellular microcompartments having an ovoid, cylindrical, spheroidal or spherical shape or a substantially ovoid, cylindrical, spheroidal or spherical shape, the smallest dimension being between 200 and 400 μm, comprising an outer hydrogel layer defining an inner portion, the inner portion comprising 500 to 5000 lymphocytes forming a grouped three-dimensional culture in a medium, the TNFα content being less than 100 ng / mL of medium. The present invention also relates to a method for producing such a microcompartment or microcompartment assembly.

The application relates to the technical field of cell culture, and discloses a cell microsphere forming device and a forming method. The cell microsphere forming device comprises a forming assembly and a filling assembly. The forming assembly comprises a first pipe body, a second pipe body and a third pipe body. The first pipe body defines a first cavity, and the first pipe body has a first end and a second end. The first cavity is communicated with the second end. The second pipe body defines a second cavity, and the second cavity is communicated with the second end. The third pipe body defines a third cavity, and the third cavity is communicated with the second cavity. The filling assembly comprises a first filling piece, a second filling piece and a third filling piece. The first filling piece is connected to the first end and defines a first filling cavity. The second filling piece defines a second filling cavity. The third filling piece defines a third filling cavity. The first filling cavity is communicated with the first cavity. The second filling cavity is communicated with the second cavity. The third filling cavity is communicated with the third cavity. The cell microsphere forming device can improve the size uniformity of the inner and outer cells and improve the culture state.

Provided are systems and methods for enriching cell-free microbial nucleic acids from one or more subjects' samples.

The invention relates to the field of three-dimensional cell culture and relates, in particular, to cellular microcompartments, without an extracellular matrix of animal origin, and / or derived from cancer cell lines, for the production of GMP-grade cells and tissues.

The invention relates to a method for separating viable target cells from a sample comprising the steps of contacting a sample comprising a suspension of viable target cells that display a molecule on the cell surface with non-porous microparticles that have a density of about 1.45 g / cm3 or greater, a diameter of about 10 μm to 200 μm, and a capture ligand covalently immobilized to the microparticle surface that is capable of specifically binding to said molecule; incubating said sample without substantial agitation to form a target cell / microparticle complex; separating non-bound substances in said sample from said target cell / microparticle complex by washing said non-bound substances through a filter while retaining said target cell / microparticle complex; mechanically dissociating said target cell / microparticle complex and eluting said viable target cells through said filter while retaining said microparticles with said capture ligand covalently immobilized to the microparticle surface as well as a cartridge, kit-of-parts, an apparatus configured to be used in the method and a medicament comprising viable target cells obtainable by the method.

Relates to the fields of biology, medicine and bioinformatics, in particular to a peripheral red blood cell micronucleus DNA separation or purification method and application thereof.

The invention discloses a microsphere based on methacrylated gelatin, a preparation method of the microsphere and application of the microsphere in bio-ink, the microsphere is prepared by taking methacrylated gelatin as a core base material and selectively compounding glycosaminoglycan-methacrylated gelatin and / or lipidosome through a microfluidic technology, the size of the obtained microsphere is uniform, and the size of the microsphere is uniform. The composite components can endow the microspheres with excellent self-adhesion characteristics; a microfluidic technology is further adopted, a base material and a composite component are used as a microsphere shell phase, a cell dispersion liquid is used as a microsphere core phase, the cell-carrying microspheres with a core-shell structure are prepared, and the cell-carrying microspheres can well maintain normal survival and growth of embedded cells. The methylacryloylated gelatin-based microspheres or cell-loaded methylacryloylated gelatin-based microspheres can be used as 3D printing ink in the fields of preparation of scaffold materials, cell-loaded scaffold materials, adaptive cell three-dimensional culture, cell ball culture, high-density cell culture and tissue engineering implant materials.

The application discloses a microfluidic chip for tissue cell nucleus spatial transcriptome sequencing, and belongs to the technical field of particle control in microfluidic chips; the microfluidic chip comprises a free transcriptome capturing layer, a micro-well layer, a code A flow channel layer, a code B flow channel layer and a nucleus transcriptome capturing layer. The micro-well layer is used for placing a tissue slice to be measured, and single cells after dissociation of the tissue slice are captured through the micro-well. The micro-well layer is attached to the free transcriptome capturing layer below, and the free transcriptome capturing layer exposed at the bottom of the micro-well is fixed with mRNA capturing sequences. The code A flow channel layer and the code B flow channel layer are used for fixing cross-type DNA microarrays on the surface of the nucleus transcriptome capturing layer. The code A flow channel layer and the code B flow channel layer are simultaneously used for fixing cross-type DNA microarrays on the free transcriptome capturing layer, so that the common sequencing of single cell spatial free transcriptome and spatial nucleus transcriptome is realized, and the blank of single cell nucleus spatial transcriptome sequencing and the common sequencing of spatial free transcriptome and spatial nucleus transcriptome is filled.

The utility model discloses a 3D cell microsphere or organoid culture well plate, including culture well plate bottom plate and culture well plate cover, and the top of culture well plate bottom plate is provided with the mounting groove, and the culture well plate cover includes the culture well plate top plate who cooperates with the mounting groove, is provided with a plurality of culture grooves on the culture well plate top plate, and the bottom of culture groove is provided with the matrix formula micropore plate, is used for the construction of 3D cell microsphere or organoid, and the lateral wall of culture groove is provided with a plurality of micropores, to solve the problem that the current liquid exchange process is easy to cause 3D cell microsphere to be moved, and to wash away.