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Detection chip determining cell alpha-naphthyl batyrate esterase and reagent thereof

A technology of naphthol butyrate esterase and detection chip, which is applied to the determination/inspection of microorganisms, specific-purpose bioreactors/fermenters, enzymology/microbiology devices, etc. Determination of cell expression and other issues, to achieve the effect of simple method

Inactive Publication Date: 2015-05-13
天津禄浩科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem that the present invention solves is to overcome in utilizing chip technology to measure cell α-naphthyl acetate esterase process because body fluid cell type is difficult to accurately measure cell expression α-naphthyl acetate esterase activity

Method used

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  • Detection chip determining cell alpha-naphthyl batyrate esterase and reagent thereof
  • Detection chip determining cell alpha-naphthyl batyrate esterase and reagent thereof
  • Detection chip determining cell alpha-naphthyl batyrate esterase and reagent thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Determination of α-naphthyl acetate esterase in chronic myeloid leukemia cells using this chip

[0034] 1. Preparation of Chips

[0035] Light-transmitting polymer materials are used, and irregular and rectangular reaction tanks are prepared by micro-processing on the base plate. The size and shape of the input and output microfluidic channels are designed according to the design requirements, and the cell capture nest is planned at the same time.

[0036] 2. Prepare Cell Suspension

[0037] According to the WHO diagnostic criteria for leukemia, clinical, blood and bone marrow cell examinations, cytogenetic examinations, and cellular immunological examinations were performed on patients to clinically diagnose chronic myelogenous leukemia, and 6 blood samples were collected from each patient. At the same time, 6 cases of healthy people with no abnormalities in clinical and laboratory examinations were set as leukemia patients as the experimental group and contro...

Embodiment 2

[0047] Example 2 Determination of α-naphthyl acetate esterase in acute myeloid leukemia cells using this chip

[0048] 1. Chip Preparation

[0049] Light-transmitting polymer materials are used, and irregular and rectangular reaction tanks are prepared by micro-processing on the base plate. The size and shape of the input and output microfluidic channels are designed according to the design requirements, and the cell capture nest is planned at the same time.

[0050] 2. Prepare Cell Suspension

[0051] According to the WHO diagnostic criteria for leukemia, clinical, blood and bone marrow cell examinations, cytogenetic examinations, and cellular immunological examinations were performed on patients to clinically diagnose acute myeloid leukemia, and 6 blood samples were collected from each patient. At the same time, 6 cases of healthy people with no abnormalities in clinical and laboratory examinations were set as the control group, and peripheral EDTA sodium salt was used to a...

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Abstract

The invention relates to the technical field of microfluidic cell chip, and discloses a detection chip determining cell alpha-naphthyl butyrate esterase and a reagent thereof. Concretely, a cell chip structure contains a cell microfluidic channel, a cell-capturing nest exist in a process of a cell passing through the channel, and is reacted with alpha-naphthyl butyrate esterase after capturing granulocyte, and thus intracellular enzyme activity is determined. The beneficial effects comprise that the chip for determining cell alpha-naphthyl butyrate esterase realizes that the activity of expressing alpha-naphthyl butyrate esterase in cells is realized by using a single cell, and makes up the blank of determining cell alpha-naphthyl butyrate esterase activity. The determinable alpha-naphthyl batyrate esterase sensitivity is 20 IU / cell or more, the whole determination process is finished within 1 hour, and the method is simple, rapid and stable.

Description

technical field [0001] The invention relates to the use of cell chip technology to measure the activity of cell α-naphthyl acetate esterase, which is used for the identification of monocytes and other cells, and provides biological information for individualized treatment of granulocyte blood diseases. It is embodied in the present invention that a cell microfluidic capture channel and a microchip structure for nested cell capture are designed, and at the same time, it contains reagents that can interact with α-naphthyl acetate esterase and are suitable for chip detection, and are used for cell α-naphthyl acetate. Enzyme detection. technical background [0002] Cell chip is a kind of biochip technology with cells as the research object. It is an emerging technology to meet the needs of exploring life sciences in the age of genes and molecules. Cell chips not only maintain the advantages of traditional cell research methods, but also meet the characteristics of high throughp...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/44
Inventor 孙续国孙朝赵鹏宋林郑芳赵萌刘金平张锦仁
Owner 天津禄浩科技股份有限公司
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