A kind of cell microcarrier matrix and its application

A technology of cell microcarriers and substrates, which is applied to cell culture supports/coatings, prostheses, microorganisms, etc., can solve the problems of wasting cell sources, cell loss, and low cell retention rate, so as to improve the effective rate and effective Facilitate adhesion and reduce damage rate

Active Publication Date: 2022-07-29
GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Cell injection is a commonly used method of injecting cells into the patient's body, but there are some defects in the process of cell injection; firstly, the loss of cells is serious. During the process of injecting target cells into the human body, the cell retention rate is lower due to tissue mechanical force. Low, the effective cells that finally reach the damaged site are insufficient; secondly, the survival rate of cells after transplantation is not high, which not only wastes the source of cells, but also consumes a large amount of cells, and directly affects the effective effect of the cells injected into the body

Method used

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  • A kind of cell microcarrier matrix and its application
  • A kind of cell microcarrier matrix and its application
  • A kind of cell microcarrier matrix and its application

Examples

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Embodiment 1

[0021] In this example, 6 groups of treatment groups were set, respectively marked as treatment 1, treatment 2, treatment 3, treatment 4, treatment 5 and treatment 6, to prepare cell microcarriers. Among the six treatment groups set in this example, different cell microcarrier matrices were used as variables, and the cell microcarrier matrix formulations used in each treatment group were shown in Table 1. The oyster shell powder used in this example is obtained by crushing and sieving oyster shells. Before use, the oyster shell powder is placed in a nuclease solution, soaked at 37° C. for 3 hours, and dried for later use.

[0022] Table 1 Composition of cell microcarrier formulas corresponding to each treatment group (mass fraction)

[0023]

[0024]

[0025] In this embodiment, the preparation method of cell microcarriers is as follows:

[0026] 1. Prepare 0.25% (w / v) initiator standard solution

[0027] Take 20mL of PBS and add it to a brown bottle (containing 0.05g ...

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Abstract

The invention provides a cell microcarrier matrix. The raw materials include methacrylated gelatin and oyster shell powder. According to the mass ratio, methacrylated gelatin: oyster shell powder=1-10:0.5-2. Methacrylated gelatin has high water content, similar composition to extracellular matrix, and has good surface activity, so it can be well compatible with cells and active factors. The carrier matrix wraps the cells in it, which can provide a three-dimensional support for the cells, and can also prevent the cells from being damaged during injection and transplantation. The compound use of methacrylated gelatin and oyster shell powder can effectively reduce the swelling rate of the cell microcarriers, so that the cell microcarriers have better mechanical strength.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular, relates to a cell microcarrier matrix and its application. Background technique [0002] Cell therapy is a method of using active cells to repair tissues and organs. The cells used for cell therapy mainly include stem cells, mature functional cells, ontology cells, xenogeneic cells and transdifferentiated cells. According to the source of cells, the cells used in cell therapy can be divided into two types, one is autologous cells. , derived from the patient's own body; the other is allogeneic cells, derived from donors. Regardless of the source of cells, in the process of application, it generally needs to go through the process of in vivo extraction, in vitro activation and culture, and infusion into the body. While cell injection is a common method of infusing cells into a patient, there are some defects in the process of cell injection; first, the loss of cells is serious, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A61L27/22A61L27/36A61L27/38A61L27/20A61L27/54A61L27/56A61L27/58
CPCA61L27/222A61L27/3604A61L27/58A61L27/56A61L27/54A61L27/227A61L27/20A61L27/38C12N5/0075A61L2400/06C12N2533/54C12N2533/18C12N2533/78C08L1/28
Inventor 曾皓宇刘园月林海珠张晓敏黄嘉莉
Owner GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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