153 results about "Cell injection" patented technology

The microfabricated cell injector having an injection wall and a cell injection needle projecting from the wall for piercing cells suspended in a fluid. The needle is held within a housing defined by the internal surfaces of the microfabricated cell injector. The housing has an inlet for suspended cells to enter and an outlet for cells to exit via the cell injection needle. A cell propulsion device is provided for impelling cells towards the needle such that, in use, cells suspended in the fluid are impelled towards the injection wall by the injection needle.

The invention discloses a three-dimensional high-flux drug screening PDMS-glass chip and the preparation method thereof, which makes uses of micro-electro processing and micro-plastic film technologies to produce the three-dimensional PDMS-glass chip with a two-layer micro-channel structure. A cell injection and waste liquid output channel of a first layer of PDMS chip is bonded with another layer of polydimethyl siloxane chip together, a drug concentration gradient channel and a quantitative cell culture chamber are arranged on the other layer of PDMS chip, the quantitative cell culture chamber is connected with an outlet channel of the drug concentration gradient channel, the quantitative cell culture chamber is respectively connected with a cell sample injection hole drilling position and a waste liquid outlet hole drilling position by the channel; the three-dimensional high-throughput drug screening PDMS-glass chip can overcome the shortcomings of heavy workload, great reagent and drug consumption, long screening period and high development cost of the traditional drug screening technology. The three-dimensional high-flux drug screening PDMS-glass chip can realize miniaturization of the chip, high flux, low cost, small reagent consumption, accurate and reliable test data and the simple design requirements of the operational process in the drug screening application on cell level.

The invention relates to the field of biology, in particular to MSC (mesenchymal stem cell) injection as well as a preparation and an application thereof. The injection comprises MSCs and a cell cryopreservation solution, wherein the cryopreservation solution comprises components in percentage by volume as follows: 25%-70% of an electrolyte balance solution, 5%-20% of clinical-grade DMSO (dimethyl sulfoxide), 1%-50% of 20% human serum albumin, 1%-10% of hydroxyethyl starch 130/0.4 and 5%-20% of triphosadenine-disodium magnesium chloride freeze-drying powder. The injection is free of animal serum, has clear ingredients and good cell cryopreservation effect and is safe and controllable, long-term storage and long-distance transport are facilitated, the survival rate of cells after recovery is higher than 95%, the vitality is high, and the injection can effectively relieve injury and inflammation symptoms of lesion tissue of lungs and promote tissue regeneration of the lungs, so that acute lung injury can be fundamentally and comprehensively treated.

The invention discloses a leak detection device and a leakage detection method for a battery shell, belonging to the field of power battery development. The leak detection equipment includes a plugging head and a vacuum system, the liquid injection port of the battery to be tested is connected to the plugging head, the vacuum system includes a pipeline, the plugging head is connected to the pipeline, the There is a vacuum pressure gauge on the pipeline. The present invention improves the sensitivity of battery casing leak detection by using a vacuum pressure gauge to test pressure changes, so that small leaks can also be detected, ensuring the pass rate of the battery casing, and at the same time has the advantages of simple operation and easy observation of detection results , Leak detection time is short and so on.

The invention provides umbilical cord mesenchymal stem cell injection as well as a preparation method and application thereof. The umbilical cord mesenchymal stem cell injection comprises mesenchymal stem cells and a frozen stock solution, wherein the frozen stock solution comprises the following components: a balanced electrolyte solution with the volume percentage of 25-70 percent, hydroxyethyl starch 130/0.4 with the mass/volume percentage (g/ml) of 1-10 percent, triphosadenine disodium magnesium chloride with the volume percentage of 5-20 percent, clinical-grade DMSO with the volume percentage of 5-20% and 20% human albumin with the volume percentage of 1-50 percent. The prepared umbilical cord mesenchymal stem cell injection is free of animal serum, safe, high in cell viability after cryopreservation resuscitation, can be used for treating inflammatory bowel disease, has a good effect and has no toxic or side effects.

The invention discloses a mixing stem cell injection and a preparation method thereof, and belongs to the field of cell preparation agents. The injection of the invention is mixture of mesenchymal stem cells from umbilical cord source and hematopoietic stem/progenitor cells from the umbilical cord blood source; the method of the mixing stem cell injection comprises the following steps: CD34+ hematopoietic stem cells are separated from the fresh umbilical cord blood and purified, and are subjected to mixed culture and amplification together with the mesenchymal stem cells from the umbilical cord source, during the amplification, the culture system is culture medium including AB umbilical cord blood plasma the volume fraction of which is 15 percent and the cell factor, 6*104 mesenchymal stem cells and CD34+ hematopoietic stem cells with the concentration of 5*104/ml are inoculated into a culture flask and are cultured for about 10 days, and two kinds of cells are obtained at the same time, so that the mixed stem cell injection including 5*105/ml of hematopoietic stem/progenitor cells and 2*105/ml of mesenchymal stem cells and containing normal saline with the volume fraction of AB umbilical cord blood plasma as 15 percent is obtained.

The invention relates to umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. The injection comprises umbilical cord mesenchymal stem cells and mixed solution; the mixed solution comprises balanced electrolyte solution, hydroxyethyl starch, adenosine disodium triphosphate-magnesium chloride, dimethyl sulfoxide and human serum albumin. The preparation method comprises the following steps: (1) fully and evenly mixing the balanced electrolyte solution, the hydroxyethyl starch and the human serum albumin, and performing filtration sterilization; (2) adding the umbilical cord mesenchymal stem cells; (3) adding the dimethyl sulfoxide; (4) performing split charging and frozen preservation to finish preparation; the invention also provides the application of the injection in the aspect of drugs for treating chronic ischemic heart diseases. The injection disclosed by the invention has a very good resuscitation effect after the frozen preservation, can be directly injected after being unfrozen and resuscitated, and has a remarkable effect on treatment on the chronic ischemic heart diseases.

The invention discloses a mesenchymal stem cell injection and a preparation method of the mesenchymal stem cell injection and an application in preparation of a medicine for treating ulcerative colitis, wherein the mesenchymal stem cell injection consists of the following components: 2*105-1*107 pieces/ml of mesenchymal stem cells, 5-10% of dimethyl sulfoxide (DMSO) by volume, 1-6% of human albumin by mass-to-volume ratio, and 1% of low molecular dextran and compound electrolyte solution. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparation of the medicine for treating ulcerative colitis, the mesenchymal stem cell injection is used for correcting immune dysfunction of a patient of ulcerative colitis, and preventing the autoimmune dysfunction from continuously damaging the intestinal tract; meanwhile, many cell growth factors and repair factors are secreted, to promote pathologically changed intestinal ulcer to heal, therefore, the purpose of foundational treating the ulcerative colitis is achieved. The disease course of the ulcerative colitis can be reversed, the patient can be helped in escaping out of influence of stomachache and diarrhea on the life as well as toxic and side effects of the medicine, so as to treat the ulcerative colitis thoroughly.

The invention relates to a cell injection for treating bone injury and a preparation method thereof, belonging to the field of tissue engineering. The injection is a compound formed by mesenchymal stem cells from autologous bone marrow, temperature sensitive gel and effective trophic factors separated and extracted from autoblood. The preparation method comprises the steps of: after extracting the bone marrow of a patient, separating and extracting the mesenchymal stem cells from the bone marrow, adding no animal serum in the amplification culture process, and mixing 1*(105-108) cells with 1-5ml of biological scaffold material to form the injectable compound. The cell injection for treating bone injury is mainly used for treating bone injuries including osteoarthritis, femoral head necrosis and delayed union and disunion of bone fracture, is convenient to use, has notable effect without immunological rejection, and can alleviate the great pain of patients caused by bone injury.

The invention provides mesenchymal stem cell injection, as well as a preparation method and application in preparing a medicament for treating children dilated cardiomyopathy. The mesenchymal stem cell injection comprises 2*10<5>-1*10<7> mesenchymal stem cells in every milliliter, clinical grade DMSO (dimethylsulfoxide) with volume ratio of 5 to 8 percent, human albumin with mass volume ratio of 1 to 6 percent, and multi-electrolyte liquid. The mesenchymal stem cell injection is prepared from placenta and umbilical cord, has the advantages of being high in yield, and being easy to realize industrialization, the preparation system quality is easy to control; the injection can be directly frozen through program-controlled freezing procedure and directly used in clinical injection after being recovered, and is safe and reliable. According to the injection, the dilated cardial structure can be changed, the function of heart can be recovered, the water supply state of each tissue in the whole body can be improved, the living quality of a patient can be improved, and the dilated cardiomyopathy can be treated fundamentally, so that the patient can get rid of serious complications caused by massive medicine administration, living action limitation and poor disease control.

The invention provides an umbilical cord mesenchymal stem cell injection with an anti-aging function and a preparation method thereof. The injection comprises the following components: umbilical cord mesenchymal stem cell (5*10<5> to 10*10<5>/mL), 2 wt% of human albumin, and 98 wt% of compound electrolyte solution. The produced umbilical cord mesenchymal stem cells can rapidly repair and replace damaged cells and tissues, and have the characteristics of amplification and directional cell differentiation; and after the stem cells receive the signals from damaged parts, the stem cells will move to the damage parts, amplify in the damage parts, carry out induced differentiation, promote the self recovery function of stem cells, rapidly repair and replace damaged cells and tissues, rapidly secrete cell factors, growth factors, and tissue related factors in time, thus improve the blood circulation, enhance the metabolism functions, improve the tissue repairing function and immunity, and finally achieve the anti-aging goal.

An automated cell injection system and method are described, which can perform automatic, reliable, and high-throughput cell injection of foreign genetic materials, proteins, and other compounds. The system and method overcome the problems inherent in traditional manual injection that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for zebrafish embryo injection but can be readily extended to other biological injection applications such as mouse embryo, drosophila embryo, and C. elegans injections, capable of facilitating high-throughput genetic research at both academic and industry levels. A novel vacuum based cell-holding device is also provided.

The invention discloses a method for high-efficiency establishment of a genetically modified animal model through haploid stem cells. Specifically, the method for high-efficiency establishment of the genetically modified animal model through haploid stem cells comprises the following steps: a) knocking out the H19 gene of male haploid embryonic stem cells through homologous recombination; b) screening and identifying the male haploid embryonic stem cells in which the H19 gene is correctly knocked out; and c) injecting the male haploid embryonic stem cells in which the H19 gene is correctly knocked out into cytoplasm of oocyte so as to produce a semi-cloned genetically modified animal. The method provided by the invention simulates the functions of sperms by establishing an H19 gene-knocked-out male haploid embryonic stem cell system so as to highly efficiently generate fertile progeny, which can then be used for implementation of novel genetic manipulation and establishment of corresponding genetically modified animal models.

Disclosed herein is a dielectric cytometric apparatus capable of analyzing cells and sorting the cells without adopting an optical analysis method and provides a dielectric cytometric cell sorting method for the apparatus. A stenosis channel allowing a single cell to flow is created on a cell injection section of a micro flow channel device used as a portion of the dielectric cytometric apparatus. A pair of measurement electrodes is created on the stenosis channel and an analyzer connected to the measurement electrodes measures the complex dielectric constant of for every cell passing through the stenosis channel. A electric-field application section provided on the downstream side of the stenosis channel applies an electric field for changing the flow of the cells inside a flow channel on the basis of information on the measured complex dielectric constants so that the cells can be sorted by making use of branch channels.

In a method for accurately delivering a therapeutic agent to a target area of an organ of a living subject, such as for injecting stem cells into the myocardium of the heart, a 3D image, in which the target area and a delivery path thereto are visible, is obtained prior to delivery of the therapeutic agent. The 3D image is displayed, and a catheter is introduced into the subject and a real time positional indication of the catheter in the subject is obtained and incorporated into the displayed image, providing visual support for guiding the catheter to the target area. When the catheter is at the target area, the therapeutic agent is injected into the target area via the catheter. The distribution of the injected therapeutic agent relative to the target area is then monitored in the displayed image.

The present invention relates to an improvement in an apparatus for detecting chemotaxis of cells. It aims at providing a structure for detecting chemotaxis of cells at an elevated accuracy with the use of a microquantity of cells. That is to say, an object of the present invention is to provide an apparatus for detecting chemotaxis of cells by which cell injection and position control can be easily carried out while ensuring the prevention of unexpected migration of the cells definitely positioned in a well or the injected sample so that a stable concentration gradient due to the diffusion of the specimen can be maintained and which ensures further automated operation and controlling. Namely, an apparatus for detecting chemotaxis of cells with a structure wherein two wells are connected to each other via a channel having resistance to the passage of cells and each well has an opening for injecting cells or a specimen, characterized by having (1) a means of transporting a liquid and a means of stopping the transportation after the injection or the aspiration discharge of the liquid and (2) a means of sealing the opening(s) in one or both of the cell-injection side and the specimen-injection side.

The invention discloses application research of an in-vivo tumor engineering human oophoroma tumor model established on the basis of an injectable collagen aquogel composite human oophoroma cells in a noninvasive subcutaneous injection form to a nude mouse. The injectable collagen aquogel stent used as a substrate material is compounded with human oophoroma cell SKOV3 to noninvasively establish the in-vivo tumor engineering human oophoroma tumor model at the nude mouse subcutaneous tissues; and compared with the traditional human oophoroma tumor model established in an independent cell injection mode under the condition of no stent material, the detection on the tumor model indicates that the malignancy of the human oophoroma tumor established on the basis of the injectable collagen aquogel is higher, and is more approximate to that of the human oophoroma tumor, and the in-vivo tumor model established in such a mode has smaller trauma possibility. The tumor model established by the method has higher tumor formation rate (up to 95%), which is about 15% higher than the traditional mode, thereby providing a more reliable tumor model for further research.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells/ 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells/ one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells/ one time/ one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The invention relates to a rotary tissue stress culture system and a method. The rotary tissue stress culture system comprises a support frame, wherein a culture liquid bottle, a multi-manifold joint, an oxygen exchanger, a defoaming device, a constant flow pump, a culture liquid pressure chamber, a multi-passage rotary joint, a bottle rotating machine, a peristaltic pump, a waste liquid bottle, a bottle rotating hydraulic chamber, a single-passage rotary joint and a control unit are arranged on the support frame, the multi-manifold joint is respectively connected with the culture liquid bottle, the oxygen exchanger and the waste liquid bottle, a plurality of passages are arranged inside the multi-passage rotary joint, two connecting openings which are arranged on the side wall of the multi-passage rotary joint are connected with two pipelines, the first pipeline is connected with a second valve inlet of the multi-manifold joint, the second pipeline is connected with the lower end of the culture liquid pressure chamber, a seed cell injection opening is arranged on the second pipeline, both ends of the bottle rotating machine are respectively provided with a top cover and a bottom cover, the top cover of the bottle rotating machine is connected with the multi-passage rotary joint, the bottom cover of the bottle rotating machine is connected with the single-passage rotary joint, the bottle rotating hydraulic chamber is connected with the single-passage rotary joint, and control signals of the control unit are respectively connected with the bottle rotating machine, the peristaltic pump, the constant flow pump and an electromagnetic valve.

The invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell injection as well as a preparation method and application thereof. The mesenchymal stem cell injection comprises mesenchymal stem cells, human serum albumins, low-molecular weight heparin calcium, vitamin C, lentinan, dimethyl sulfoxide and a menstruum. The mesenchymal stem cell injection provided by the invention can effectively maintain the activity of the stem cells, can increase the content of striatal dopamine and can effectively treat the Parkinson's disease.