24 results about "Cell injection" patented technology

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The invention relates to the technical field of acoustic wave device application and biological tissue engineering, in particular to a method for constructing a multi-component heterogeneous fiber structure under the assistance of acoustic tweezers. According to the method, an acoustic tweezers technology and a microfluidic spinning technology are combined, an electric signal is converted into an acoustic surface wave through the piezoelectric effect of a piezoelectric material, and a beam-shaped acoustic standing wave field is formed in a microchannel of a specific structure; different target cells form a stable fiber configuration under the interaction of microchannel limitation and acoustic radiation force, and spatial precise classification and arrangement of multiple types of cells are realized. The low-temperature-sensitive hydrogel is introduced in the cell injection process, and the fiber scaffold is formed after ultraviolet curing, so that the fiber structure of the cells is still maintained after the sound field is removed. The cells treated by the surface acoustic wave can form a complex three-dimensional bionic tissue structure more easily, and the cell viability is remarkably improved. Various fiber structures can be formed by regulating and controlling sound field parameters, and great potential is shown in the field of in-vitro barrier model construction.

The present disclosure provides for one or more embodiments for treating retinal degenerative disorders in a subject by administering to the subject an effective amount of allogeneic mesenchymal stem cells to the subject's suprachoroidal area. Allogeneic mesenchymal stem cells, when used in the disclosed treatment regimen, may exhibit anti-inflammatory, immunomodulatory, anti-fibrotic, pro-vascular, and / or pro-regenerative processes. Thus, allogeneic mesenchymal stem cells may be understood to have the potential to target and treat multiple pathological targets implicated in retinal degenerative disorders, including retinal disorders characterized by drusen accumulation, some of which have inherited forms.

The utility model relates to the technical field of dosing devices, and discloses a stem cell injection dosing device which comprises a needle cylinder, an observation window is fixedly installed on the outer surface of the needle cylinder, a needle head is movably installed on the right surface of the needle cylinder, and a protection mechanism is arranged on the needle cylinder. The protection mechanism comprises a hollow column, a protection sleeve, a sealing groove, a clamping hole, a needle head, a push rod, a push handle, a piston head, a rubber sealing ring, a sealing gasket, a sliding groove and two springs, according to the stem cell injection dosing device, the hard protection sleeve is installed in a clamping mode to protect the thin needle head, the needle head is prevented from being bent due to external factors, and the safety of the stem cell injection dosing device is improved. A sealing groove formed in the outer surface of the needle cylinder is filled with a rubber sealing ring fixedly installed on the inner surface of the hollow column after installation for sealing treatment, germs are prevented from entering the protective sleeve and polluting the needle head, meanwhile, the needle head is inserted into a sealing gasket fixedly installed in the inner wall of the protective sleeve for sealing, germs are prevented from being carried, and protection on the needle head is improved.

The present application relates to a kind of extracellular matrix-cell complex for inhibiting intervertebral disc degeneration and its preparation method and application, the extracellular matrix-cell complex for inhibiting intervertebral disc degeneration includes modified cell and the biomimetic extracellular matrix nanofiber constructed on the surface of modified cell.The present application is creatively modified to implant cell in cell therapy, constructs artificial extracellular matrix with nanofiber characteristics in situ on the surface of cell, the complex is implanted into nucleus pulposus by cell injection therapy, can induce cell rest, so that implanted cell is better adapted to the intracellular environment of nucleus pulposus anoxia.The artificial extracellular matrix can be long-term targeted to cell surface, significantly prolongs the action time, and different modified cells can be selected according to actual needs, so that intervertebral disc degeneration cell therapy can be better realized, with wide application prospect.

Collected stem cells are activated by treating them with a wavelength-modulated laser beam having a wavelength ranging from 405 to 980 nanometers. The frequency of the laser beam is modulated within a range of 8 to 12 MHz. Using the activated stem cells, tissue can be repaired and regenerated by preparing non-activated stem cells, treating the non-activated stem cells with a wavelength-modulated laser beam having a predetermined frequency to obtain activated stem cells, administering the activated stem cells into a body containing the tissue, and using a targeting beam to guide the activated stem cells within the body to the location of the tissue.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.

The invention provides a construction method of an embryo-like model, which comprises the following steps: culturing primitive state stem cells in vitro or injecting the primitive state stem cells into an immunodeficient animal body for differentiation to obtain the embryo-like model. According to the method, in the process of preparing the embryo-like model, cell factors and small molecular pathway interference factors do not need to be added, gene editing is not needed, only a single starting cell type is adopted, and the method has the advantages of being simple and high in repeatability, facilitates large-scale preparation and application, and has wide application prospects. And the prepared embryo-like model has all embryo external cell lineages and all embryo internal cell lineages except for trophoderm, and can simulate the development process from an implanted embryo of a Carbonate-resistant group 3 stage to a Carbonate-resistant group 9 stage nerve embryo, so that the embryo-like model can be used for preparing various progenitor cell lineages, modeling diseases, screening drugs and carrying out scientific research.

The invention belongs to the technical field of biomedical analysis, and relates to a cell sorting and detecting system integrating microfluidic control and a terahertz transmission line and a method thereof. A cell sorting area of the system comprises: a cell injection chamber for introducing a multi-cell mixed culture solution to be sorted; the microfluid channel is used for realizing primary cell sorting based on a magnetic field; the capture trap microstructure array is used for capturing the free MNPs and realizing the separation of the free MNPs and the target cells with the MNPs markers; the transmission line detection area comprises a fixing structure used for fixing a to-be-detected cell in a sensing area of the transmission line; the transmission line detection unit is used for detecting the characteristic spectrums of the labeled cells and the unlabeled cells in situ in real time; an outlet collection chamber is also included. High integration of cell sorting and electrical detection is achieved, high-sensitivity detection of terahertz waves and accurate sample control of microfluidics can be combined, and higher-throughput, lower-damage and more accurate sample analysis and sorting are achieved.

The invention relates to the technical field of medicine, in particular to a preparation method of a human umbilical cord mesenchymal stem cell injection and a product thereof, and the preparation method at least comprises the following steps: obtaining P0-generation secondary human umbilical cord mesenchymal stem cells; and carrying out inoculated culture on the P0-generation human umbilical cord mesenchymal stem cells, carrying out cell digestion, centrifugation and washing, and then carrying out resuspension by using an auxiliary material solution to obtain the human umbilical cord mesenchymal stem cell injection. The human umbilical cord mesenchymal stem cells obtained by screening and culturing the primary separation, the culture medium, the inoculation density, the centrifugal parameters, the culture container, the digestive juice, the washing conditions and the like have higher cell viability and more stable cell growth characteristics. According to the preparation method of the human umbilical cord mesenchymal stem cell injection provided by the invention, the high-quality and high-activity cell injection can be prepared, and a reliable guarantee is provided for clinical application.

The application provides a mesenchymal stem cell cryopreservation solution which can be directly transfused into human body, the cryopreservation solution comprises dimethyl sulfoxide with a mass concentration greater than 0% and less than 5%, carboxymethyl cellulose salt with a mass concentration greater than 0% and less than or equal to 0.8%, and compound electrolyte injection solution. The mesenchymal stem cell cryopreservation solution of the application adopts clinical grade reagent, does not contain serum and animal-derived components, and the cell injection solution after cell cryopreservation and recovery can be directly transfused into human body, so that cell damage and loss caused by the washing process are significantly reduced, and the application is convenient. The carboxymethyl cellulose salt in the cryopreservation solution effectively replaces the protein protective agent, significantly reduces the dosage of dimethyl sulfoxide, has very low toxic side effects and irritability, and can ensure excellent 0-hour and 24-hour cell survival rate and recovery rate, and the cells after recovery can be placed at room temperature for a long time, and have good room temperature stability; meanwhile, the cryopreservation cost of the mesenchymal stem cells is greatly reduced, and the application is suitable for large-scale clinical application.

The invention discloses a directional blood flow training composite dilator, and relates to the technical field of medical instruments. The device comprises an expansion bag, one side of the expansion bag is communicated with a water injection connecting pipe, the other end of the water injection connecting pipe is communicated with a water injection kettle, and one side of the expansion bag is provided with a stem cell injection kettle. The hard base plate structure is arranged, the rigid characteristic of the hard base plate can restrain the expansion bag to only expand towards the upper soft tissue direction, directional controllable mechanical stress is formed, a one-way expansion mode promotes new blood vessels to grow in order in the preset direction, the blood vessel density and blood supply quality of the expanded skin flap are remarkably improved, and the expansion effect is good. The design of the uniformly distributed grooves provides an accurate positioning reference for blood flow training, so that a blood supply path can be accurately controlled during follow-up blood flow blocking training through instruments such as an elastic band, finally, an axial flap with a clear vascular pedicle is formed, and the survival rate and the tissue utilization rate of the flap after transplantation are greatly improved.

The invention relates to the technical field of injection equipment, in particular to a mouse cell injection device which comprises an operation table, a plurality of mounting seats are arranged on the upper surface of the operation table, lifting lead screws are vertically and rotatably arranged on the mounting seats, and a mounting frame is arranged on the upper portions of the lifting lead screws; a driving motor used for driving lifting lead screws is arranged on the upper portion of the mounting frame, a lifting block is arranged on a rod body of each lifting lead screw, two sets of sliding rods are arranged between the lifting blocks, a cross beam is arranged on the sliding rods in a sliding mode, and a sliding table used for fixing an injector is arranged on the upper surface of the cross beam in a sliding mode. A positioning device for positioning a blood vessel is arranged on the mounting frame, and the working end of the sliding table faces the positioning device, so that physiological shaking of a person is effectively eliminated, an injection needle can be accurately aligned with a blood vessel positioning point in a three-dimensional space, and the positioning accuracy is improved.

The application provides a method for genetic reactivation of individual Procyprinus palpebrosus, comprising the following steps: S1, taking the gonadal tissue of Procyprinus palpebrosus, washing, cutting, adding L-15 culture medium containing double antibodies and DPBS buffer solution to centrifuge the gonadal tissue; S2, water-bathing the gonadal tissue to obtain recovered gonadal tissue; S3, digesting the recovered gonadal tissue, filtering, centrifuging and purifying to obtain germ stem cells; S4, injecting morpholino to obtain rare gobi-ocypris rarus with deleted primordial germ cells; S5, transplanting the germ stem cells into the body of the rare gobi-ocypris rarus juvenile to culture, and obtaining the gametes of Procyprinus palpebrosus; and S6, fertilizing the generated gametes of Procyprinus palpebrosus to obtain the individual Procyprinus palpebrosus. The application successfully obtains the sperm of Procyprinus palpebrosus by using the cryopreserved gonadal tissue and the technology of parthenogenesis, and provides a feasible way for the individual genetic reactivation of rare and endangered fish species.

The utility model discloses a mesenchymal stem cell injection preservation device, which relates to the technical field of medical equipment and comprises a preservation box, a box cover, a mounting frame, a fixing plate, an adjusting seat, a fixing seat, a bearing cylinder and a series of transmission and support components. Wherein the storage box serves as a shell of the device and is used for containing internal components and forming a closed space; the box cover is matched with the storage box to prevent external pollution. Furthermore, the mounting frame is arranged in the storage box and provides a mounting foundation for other components, and the fixing plate is mounted on the inner wall of the storage box and provides support for the components such as the sliding hole and the sliding block. The position of the adjusting seat is adjusted through the cooperation of the sliding block and the sliding hole so as to adapt to injection containers of different sizes. According to the mesenchymal stem cell injection preservation device, the position can be accurately adjusted, the mesenchymal stem cell injection preservation device adapts to injections with different thicknesses, comprehensive protection is provided, transportation stability and safety are ensured, and the preservation time of the injections is effectively prolonged.

The utility model relates to cell biomedical technology field discloses a kind of cell injection liquid anti-bunching oscillation device, including vibration plate, the vibration plate upper surface is fixedly connected with stand one, the stand one outer wall is fixedly connected with connecting block one, the connecting block one outer wall is fixedly connected with fixed box, the fixed box upper surface is provided with clamping assembly;The clamping assembly includes multiple clamping plate one and clamping plate two, the clamping plate one and clamping plate two lower surface are all fixedly connected with fixed block one, the fixed block one inner wall is slidably connected with connecting rod, the clamping plate one and clamping plate two inner wall sliding joint include multiple clamping plate one and clamping plate two, the clamping plate one and clamping plate two lower surface are all fixedly connected with fixed block one limit rod.The utility model in, by setting up the clamping assembly of reasonable structure on vibration plate, utilize rack-gear linkage structure between clamping plate one and clamping plate two, realized the synchronous adjustable of jig spacing.

The application discloses a visual puncture needle assembly and system for stem cell injection, and belongs to the technical field of stem cell treatment surgical instruments. The visual puncture needle system comprises a visual puncture needle assembly and a collection display system. The visual puncture needle assembly comprises a puncture needle, a visual sleeve, a plurality of camera probes and a plurality of laser ranging probes. The collection display system is in communication connection with the plurality of camera probes and the plurality of laser ranging probes. The collection display system is used for acquiring internal image picture information of a lesion cavity and displaying the internal image picture of the lesion cavity. Not only can the internal condition of the lesion cavity be observed, but also the position of the laser ranging probe in the lesion cavity can be adjusted. The collection display system is also used for acquiring internal size information of the lesion cavity, calculating the volume of the lesion cavity according to the internal size information of the lesion cavity, and then obtaining the dosage of stem cell injection, so that the purpose of precise treatment is achieved.

The invention belongs to the technical field of cell sorting and cell detection, and relates to a cell sorting and real-time detection system and a cell sorting and real-time detection method combining microfluidics and a uniform-spacing microelectrode array. The system comprises a cell injection chamber, a microfluid channel, a capture trap microstructure array, a cell collection chamber and a microelectrode array, the microelectrode array comprises a hexagonal electrode array and a sinusoidal line electrode array, the side length of the hexagonal electrode array and the cell size meet L = kDc, and the hexagonal electrode array meets d =; the amplitude A of the sine line electrode array is lambda / 4, and the wavelength lambda is two times of the cell diameter. Microfluidic sorting and a microelectrode detection array are combined, two innovative interdigitated electrode structures are adopted, the detection sensitivity is remarkably improved, and the micro-fluidic chip is particularly suitable for cell sorting, cell monitoring, precise diagnosis and biosensing application; an integrated and high-throughput solution is provided for the fields of early diagnosis of cancers, drug screening, personalized medical treatment and the like.

This utility model relates to a packaging bag for stem cell injection solution. The packaging bag includes a bag body and an infusion interface. The bag body includes a light-proof area and a transparent window area. The inner layer of the light-proof area is polytetrafluoroethylene (PTFE), and the outer layer is a dark-colored coating or dark-colored high-density polyethylene (HDPE). A sealing strip is provided between the transparent window area and the light-proof area. Compared with existing technologies, this packaging bag is specifically designed for the biochemical characteristics of cardiac stem cells and their injection solution. Using HDPE as the main material, it has good biocompatibility, non-stick properties, and thermal insulation, ensuring both the safety and stability of stem cell storage. The sealing strip between the transparent window area and the light-proof area prevents the injection solution from being exposed to the transparent area and deteriorating before use. A Luer-Lock interface with a double-sealing structure (sealing ring or threaded structure) ensures that the stem cell injection solution is leak-free and uncontaminated during storage and transportation.

1. Name of the designed product: Real-time microscopic observation equipment for stem cell injection therapy. 2. Use of the designed product: Real-time microscopic observation of ongoing stem cell injection therapy. 3. Design points of the designed product: In shape. 4. Picture or photo best indicating the design points: Perspective view.

To provide a cell injection device and a cell injection method capable of efficiently injecting a substance into a cell mass.SOLUTION: A cell injection device includes a first tubular micro tool that has a first opening provided at a distal end and a first hollow portion communicating with the first opening and is capable of holding a cell aggregate in the first opening, a second tubular micro tool that has a second opening provided at a distal end and a second hollow portion communicating with the second opening and is capable of injecting a substance accommodated in the second hollow portion into the cell aggregate from the second opening, and a fixing portion that forms an insertion path wider than an outer diameter of the second micro tool and narrower than a diameter of the cell aggregate. The second micro tool has an outer diameter smaller than an inner diameter of the first micro tool and is capable of holding the cell aggregate held by the first micro tool by penetrating the cell aggregate in the axial direction, and when the second micro tool that has penetrated the cell aggregate is arranged in the insertion path, the fixing part is arranged on the base end side in the axial direction with respect to the cell aggregate penetrated by the second micro tool.SELECTED DRAWING: Figure 12

The invention discloses an autologous stem cell injection method for knee osteoarthritis treatment, and relates to the technical field of cell treatment and orthopedic disease intervention, and the autologous stem cell injection method comprises the following steps: collecting a broadband noise signal, subharmonic amplitude and ultrasonic excitation power recorded by an acoustic monitoring unit in real time, carrying out unified timestamp synchronization on the obtained original data, and carrying out time stamp synchronization; and obtaining a complete acoustic observation sequence. According to the method, acoustic monitoring, image observation and cell activity signals are fused, a cross-modal risk vector is constructed, a Mahalanobis distance and exponential smoothing algorithm is introduced for accurate prediction, and the risk effectiveness is judged in combination with chi-square test, microsecond-level phase modulation intervention is triggered, a sound field standing wave structure is actively disturbed, and cavitation formation is inhibited. The scheme has the advantages of quick response, high-precision recognition and prospective control, the activity of stem cells is effectively protected, the injection treatment effect is improved, and intelligent, safe and efficient technical support is provided for knee osteoarthritis regenerative medicine.

1. The name of the design product: split stem cell injection therapy real-time microscopic observation equipment. 2. The use of the design product: using split screen design, real-time microscopic observation of stem cells in the infusion tube dropper during stem cell injection therapy. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.