51 results about "Cell injection" patented technology

The invention relates to the technical field of stem cell injections, in particular to a human umbilical cord mesenchymal stem cell injection and a preparation method thereof. The cell cryoprotectant comprises the following components: a cell cryoprotectant and P6-generation human umbilical cord mesenchymal stem cells. By selecting P6-generation human umbilical cord mesenchymal stem cells, the capacity of secreting hepatocyte growth factors (HGF) can be improved; the cell cryoprotectant contains 6-9wt% of dimethyl sulfoxide and a certain amount of human serum albumin, can better protect cells and maintain the motility rate and biological functions of the cells, and is more beneficial to preparation of cryopreserved human umbilical cord mesenchymal stem cell injection. The P6-generation human umbilical cord mesenchymal stem cells are obtained by culturing a culture medium containing fetal calf serum, and the ability of the injection to repair liver injury can be improved.

To provide a cell injection device, a cell collection accessory jig, and a cell collection method capable of stably injecting foreign cells into a cell mass regardless of the skill and technique of an operator.SOLUTION: A cell injection device includes: a cell collection accessory jig 24 including a filter section 26 having bubbles of a size that allows a liquid medium D to pass through but not cells Ce; an operation manipulator equipped with an injection pipette 90 capable of collecting and discharging a plurality of cells Ce and the liquid medium D from an opening 90a at the tip; an injection pump capable of adjusting the internal pressure of the injection pipette 90; and a controller that, after collecting a plurality of cells Ce and the liquid medium D inside the injection pipette 90, applies positive pressure to the inside of the injection pipette 90 while pressing the opening 90a at the tip of the injection pipette 90 against the filter section 26, thereby discharging the liquid medium D through the filter section 26 and aligning the exogenous cells Ce inside the injection pipette 90 in rosary-like pattern.SELECTED DRAWING: Figure 2

The present application discloses a method for injecting exogenous substances into cells based on a single-cell operation device. The single-cell operation device includes a stage carrying a sample, a first probe, a first electrode arranged on the first probe, and a second electrode electrically connected to the sample. The first probe is an elongated hollow structure and includes a front end and a rear end. The front end of the first probe acts on the cell membrane of the target cell in the sample. The method for injecting exogenous substances into cells includes: step S100, establishing a first electric field between the first electrode and the second electrode, and the first probe detects the change of the first electric field signal during the movement to determine whether the front end of the first probe is in contact with the target cell; step S200, the front end of the first probe moves relative to the sample to penetrate the cell membrane of the target cell; step S300, establishing a second electric field between the first electrode and the second electrode, and the exogenous substance is injected into the target cell under the action of the second electric field. The technical solution in the present application has the advantages of high precision, low damage, high throughput, and multifunctionality.

The invention provides a mesenchymal stem cell injection, a preparation process and application of the mesenchymal stem cell injection to treatment of autoimmune diseases, and belongs to the technical field of biological medicines. The mesenchymal stem cell injection provided by the invention is prepared from a complete culture solution 1, a complete culture solution 2 and a complete culture solution 3 under specific culture conditions. The mesenchymal stem cell working bank used for preparing the mesenchymal stem cell injection is good in cell safety, has better cell proliferation ability, highly expresses CD73, CD90 and CD105, does not express CD19, CD34, CD45, CD11b and HLA-DR, and has osteogenesis and adipogenesis differentiation ability and better biological efficacy. The mesenchymal stem cell injection provided by the invention meets the pharmacopoeia standard, has high HGF and sTNFR1 contents, and can play a role in preventing and treating autoimmune diseases.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The invention relates to the technical field of acoustic wave device application and biological tissue engineering, in particular to a method for constructing a multi-component heterogeneous fiber structure under the assistance of acoustic tweezers. According to the method, an acoustic tweezers technology and a microfluidic spinning technology are combined, an electric signal is converted into an acoustic surface wave through the piezoelectric effect of a piezoelectric material, and a beam-shaped acoustic standing wave field is formed in a microchannel of a specific structure; different target cells form a stable fiber configuration under the interaction of microchannel limitation and acoustic radiation force, and spatial precise classification and arrangement of multiple types of cells are realized. The low-temperature-sensitive hydrogel is introduced in the cell injection process, and the fiber scaffold is formed after ultraviolet curing, so that the fiber structure of the cells is still maintained after the sound field is removed. The cells treated by the surface acoustic wave can form a complex three-dimensional bionic tissue structure more easily, and the cell viability is remarkably improved. Various fiber structures can be formed by regulating and controlling sound field parameters, and great potential is shown in the field of in-vitro barrier model construction.

The invention discloses a testis Leydig cell senescence marker and application thereof, the senescence marker is CCN5 protein, the senescence condition of the Leydig cell can be specifically reflected by detecting the CCN5 level of testis tissue, the senescence marker can be used as an index for evaluating Leydig cell senescence and testis senescence, and meanwhile, the senescence marker is an effective supplement for a diagnostic scheme of late onset hypogonadism (LOH). Furthermore, the invention also provides application of a preparation for inhibiting CCN5 protein expression in preparation of a product for improving testis Leydig cell senescence or treating diseases caused by testis Leydig cell senescence, the testis Leydig cell senescence can be improved and the LOH symptom can be treated by reducing CCN5 in a targeted manner, and treatment can be performed aiming at the core phenotypes of'lipid droplet loss' and'fibrosis' of testis Leydig cell senescence. The method is a treatment scheme different from exogenous testosterone supplement, stem cell injection, Senlytics drugs and exosome therapy.

The present disclosure provides for one or more embodiments for treating retinal degenerative disorders in a subject by administering to the subject an effective amount of allogeneic mesenchymal stem cells to the subject's suprachoroidal area. Allogeneic mesenchymal stem cells, when used in the disclosed treatment regimen, may exhibit anti-inflammatory, immunomodulatory, anti-fibrotic, pro-vascular, and / or pro-regenerative processes. Thus, allogeneic mesenchymal stem cells may be understood to have the potential to target and treat multiple pathological targets implicated in retinal degenerative disorders, including retinal disorders characterized by drusen accumulation, some of which have inherited forms.

The invention provides a cryopreservation protective solution for CIK (cytokine-induced killer) cells. The cryopreservation protective solution for a CIK cell injection comprises the following components in percentage by volume: 5-10% of dimethyl sulfoxide, 20-40% of human serum albumin and the balance of compound electrolyte. The cryopreservation protection liquid for the CIK cells is simple in component and low in raw material cost, the safety risk caused by high-concentration DMSO is reduced, meanwhile, the cell viability after resuscitation of the cryopreserved cells is improved, the CIK cells are cryopreserved through the cryopreservation protection liquid, the CIK cells can be directly used after being unfrozen and centrifugally cleaned, and activation culture does not need to be conducted again.

The utility model relates to the technical field of dosing devices, and discloses a stem cell injection dosing device which comprises a needle cylinder, an observation window is fixedly installed on the outer surface of the needle cylinder, a needle head is movably installed on the right surface of the needle cylinder, and a protection mechanism is arranged on the needle cylinder. The protection mechanism comprises a hollow column, a protection sleeve, a sealing groove, a clamping hole, a needle head, a push rod, a push handle, a piston head, a rubber sealing ring, a sealing gasket, a sliding groove and two springs, according to the stem cell injection dosing device, the hard protection sleeve is installed in a clamping mode to protect the thin needle head, the needle head is prevented from being bent due to external factors, and the safety of the stem cell injection dosing device is improved. A sealing groove formed in the outer surface of the needle cylinder is filled with a rubber sealing ring fixedly installed on the inner surface of the hollow column after installation for sealing treatment, germs are prevented from entering the protective sleeve and polluting the needle head, meanwhile, the needle head is inserted into a sealing gasket fixedly installed in the inner wall of the protective sleeve for sealing, germs are prevented from being carried, and protection on the needle head is improved.

The invention provides a micro-fluidic chip for cell identification, cell culture and drug response monitoring and a use method. The chip comprises a substrate, a first electrode, a second electrode, a third electrode and a fourth electrode, the cell injection layer is arranged on the substrate, and a cell recognition assembly is arranged on the cell injection layer and used for recognizing the type of injected cells; a cell monitoring component is arranged on the cell injection layer and is used for culturing injected cells, performing different medication on the cultured injected cells according to the types of the injected cells, and monitoring the medication reaction of the cultured injected cells. According to the micro-fluidic chip for identifying and culturing the cells and monitoring the medication reaction and the use method of the micro-fluidic chip, the cell type is identified through the cell identification assembly, medication is determined according to the cell type, targeted medication is performed on different types of cells through the cell monitoring assembly, and the medication reaction is monitored, so that the medication reaction of the cells is monitored, and the accuracy of the medication reaction is improved. The micro-fluidic chip integrating identification and medication monitoring is realized.

The invention discloses a method for reducing stem cell injection loss and improving injection accuracy, which comprises the following steps: determining radioactive rectal injury diagnosis and treatment indications according to clinical medical history and previous examination, determining typical focuses in intestinal tracts through enteroscopy, preparing a stem cell preparation, and performing multi-point injection administration on typical lesion parts. Air in the endoscope injection tube is emptied before injection; a submucous membrane water cushion is formed by utilizing the endoscope injection tube; a vertical injection method is adopted for dosing and air is used for boosting; according to a preset formula, the injection dosage of each diseased region and each injection site is calculated, the injection speed is controlled, the injection dosage is finely adjusted according to the diseased condition, and the endoscope is retreated after injection is finished; the problems of dose loss and inaccurate injection existing in stem cell injection in radioactive rectal injury treatment are solved, and the effect and reliability of stem cell treatment are improved.

The present application relates to a kind of extracellular matrix-cell complex for inhibiting intervertebral disc degeneration and its preparation method and application, the extracellular matrix-cell complex for inhibiting intervertebral disc degeneration includes modified cell and the biomimetic extracellular matrix nanofiber constructed on the surface of modified cell.The present application is creatively modified to implant cell in cell therapy, constructs artificial extracellular matrix with nanofiber characteristics in situ on the surface of cell, the complex is implanted into nucleus pulposus by cell injection therapy, can induce cell rest, so that implanted cell is better adapted to the intracellular environment of nucleus pulposus anoxia.The artificial extracellular matrix can be long-term targeted to cell surface, significantly prolongs the action time, and different modified cells can be selected according to actual needs, so that intervertebral disc degeneration cell therapy can be better realized, with wide application prospect.

Collected stem cells are activated by treating them with a wavelength-modulated laser beam having a wavelength ranging from 405 to 980 nanometers. The frequency of the laser beam is modulated within a range of 8 to 12 MHz. Using the activated stem cells, tissue can be repaired and regenerated by preparing non-activated stem cells, treating the non-activated stem cells with a wavelength-modulated laser beam having a predetermined frequency to obtain activated stem cells, administering the activated stem cells into a body containing the tissue, and using a targeting beam to guide the activated stem cells within the body to the location of the tissue.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

The invention relates to a mesenchymal stem cell injection and a preparation method thereof. The injection takes 18AA-V-SF compound amino acid injection as a mother solution, and contains 0.375 to 6 [mu] g / mL of coenzyme Q10, 0.25 to 4 ng / mL of vitamin B2 and 0.5 to 8 Um / 10 ml of low molecular weight heparin sodium. Compared with the prior art, the injection obviously enhances the stability and activity of stem cells in in-vitro culture and clinical transplantation. Tests prove that the high-concentration, medium-concentration and relatively low-concentration injection combined with human umbilical cord mesenchymal stem cells has an anti-inflammatory effect on H9C2 cell LPS damage, and the anti-inflammatory effect of a medium-concentration group is obviously superior to that of other groups. The injection is an efficient and safe umbilical cord mesenchymal stem cell injection for treating myocardial infarction, and has great clinical application potential.

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.

A cell and tissue sheet forming package includes a container body, a membrane, a sliding door plate and a sealing film. The sliding door plate is disposed slidably on a top of the container body to cover or expose the membrane. The sliding door plate has a hole and a passive magnetic assembly. The cell injection equipment includes a carrier, an injection mechanism and a drive mechanism. The carrier carries the package, and the drive mechanism moves the carrier and the injection mechanism to have the injection mechanism to inject a solution, through the hole, into the package. A heating element of the carrier is introduced to heat the membrane and the solution to transform the solution into a colloid sheet on the membrane. Then, the positive magnetic assembly engages magnetically the passive magnetic assembly to slide the sliding door plate to expose the colloid sheet on the membrane.

The invention provides a construction method of an embryo-like model, which comprises the following steps: culturing primitive state stem cells in vitro or injecting the primitive state stem cells into an immunodeficient animal body for differentiation to obtain the embryo-like model. According to the method, in the process of preparing the embryo-like model, cell factors and small molecular pathway interference factors do not need to be added, gene editing is not needed, only a single starting cell type is adopted, and the method has the advantages of being simple and high in repeatability, facilitates large-scale preparation and application, and has wide application prospects. And the prepared embryo-like model has all embryo external cell lineages and all embryo internal cell lineages except for trophoderm, and can simulate the development process from an implanted embryo of a Carbonate-resistant group 3 stage to a Carbonate-resistant group 9 stage nerve embryo, so that the embryo-like model can be used for preparing various progenitor cell lineages, modeling diseases, screening drugs and carrying out scientific research.

The invention belongs to the technical field of biomedical analysis, and relates to a cell sorting and detecting system integrating microfluidic control and a terahertz transmission line and a method thereof. A cell sorting area of the system comprises: a cell injection chamber for introducing a multi-cell mixed culture solution to be sorted; the microfluid channel is used for realizing primary cell sorting based on a magnetic field; the capture trap microstructure array is used for capturing the free MNPs and realizing the separation of the free MNPs and the target cells with the MNPs markers; the transmission line detection area comprises a fixing structure used for fixing a to-be-detected cell in a sensing area of the transmission line; the transmission line detection unit is used for detecting the characteristic spectrums of the labeled cells and the unlabeled cells in situ in real time; an outlet collection chamber is also included. High integration of cell sorting and electrical detection is achieved, high-sensitivity detection of terahertz waves and accurate sample control of microfluidics can be combined, and higher-throughput, lower-damage and more accurate sample analysis and sorting are achieved.

The invention relates to the technical field of medicine, in particular to a preparation method of a human umbilical cord mesenchymal stem cell injection and a product thereof, and the preparation method at least comprises the following steps: obtaining P0-generation secondary human umbilical cord mesenchymal stem cells; and carrying out inoculated culture on the P0-generation human umbilical cord mesenchymal stem cells, carrying out cell digestion, centrifugation and washing, and then carrying out resuspension by using an auxiliary material solution to obtain the human umbilical cord mesenchymal stem cell injection. The human umbilical cord mesenchymal stem cells obtained by screening and culturing the primary separation, the culture medium, the inoculation density, the centrifugal parameters, the culture container, the digestive juice, the washing conditions and the like have higher cell viability and more stable cell growth characteristics. According to the preparation method of the human umbilical cord mesenchymal stem cell injection provided by the invention, the high-quality and high-activity cell injection can be prepared, and a reliable guarantee is provided for clinical application.

The invention belongs to the technical field of biological medicines, and particularly relates to an amniotic mesenchymal stem cell injection and a large-scale construction method thereof. The amniotic mesenchymal stem cell injection is prepared from amniotic mesenchymal stem cells, a compound electrolyte injection, hydroxyethyl starch, low-molecular-weight heparin calcium, vitamin C, trehalose, ophiopogonic acid, ganoderma capense glycopeptide and water. The amniotic mesenchymal stem cell injection provided by the invention can effectively maintain the cell viability and osteogenic differentiation capacity of amniotic mesenchymal stem cells, and additives are clinically common drugs, so that the amniotic mesenchymal stem cell injection is safe and has no side effects.

The utility model discloses a device for reducing clotting of clinical cell injection, which comprises a base, an object carrying plate and a protective cover, an adapter is placed on the upper surface of the object carrying plate, the protective cover is covered above the object carrying plate, a sliding plate is slidably connected in the protective cover, the lower surface of the sliding plate is slidably connected with a plurality of pressing plates through first springs, and the lower surface of the sliding plate is provided with a plurality of second springs. According to the utility model, through the design of the replaceable adapter, the device can be flexibly adapted to bagged or bottled cell injections with different specifications and shapes, so that the application range is greatly widened, special equipment does not need to be customized for each container, the use cost is reduced, and the universality and flexibility of the equipment are improved; the stability of the cell injection in the uniform shaking process is achieved through the synergistic effect of the protective cover, the fixing plate, the pressing plate and other assemblies, meanwhile, the pressing handle is ingeniously combined with the clamping rod and the clamping groove, the whole fixing and unlocking process is simple and rapid, an operator can conveniently and rapidly complete equipment mounting and dismounting, and the working efficiency is improved.

The invention discloses a visual puncture needle assembly and system for stem cell injection, and belongs to the technical field of stem cell treatment surgical instruments. The visual puncture needle system comprises a visual puncture needle assembly and an acquisition and display system, the visual puncture needle assembly comprises a puncture needle, a visual sleeve, a plurality of camera probes and a plurality of laser ranging probes, and the acquisition and display system is in communication connection with the camera probes and the laser ranging probes. The collection and display system is used for obtaining internal image picture information of the focus sac cavity and displaying the internal image picture of the focus sac cavity, the internal condition of the focus sac cavity can be observed, the position of the laser ranging probe in the focus sac cavity can be adjusted in an auxiliary mode, and the collection and display system is further used for obtaining internal size information of the focus sac cavity and displaying the internal size information of the focus sac cavity. And the volume of the focus sac cavity is calculated according to the internal size information of the focus sac cavity, so that the dosage of stem cell injection is obtained, and the purpose of precise treatment is achieved.

The application provides a mesenchymal stem cell cryopreservation solution which can be directly transfused into human body, the cryopreservation solution comprises dimethyl sulfoxide with a mass concentration greater than 0% and less than 5%, carboxymethyl cellulose salt with a mass concentration greater than 0% and less than or equal to 0.8%, and compound electrolyte injection solution. The mesenchymal stem cell cryopreservation solution of the application adopts clinical grade reagent, does not contain serum and animal-derived components, and the cell injection solution after cell cryopreservation and recovery can be directly transfused into human body, so that cell damage and loss caused by the washing process are significantly reduced, and the application is convenient. The carboxymethyl cellulose salt in the cryopreservation solution effectively replaces the protein protective agent, significantly reduces the dosage of dimethyl sulfoxide, has very low toxic side effects and irritability, and can ensure excellent 0-hour and 24-hour cell survival rate and recovery rate, and the cells after recovery can be placed at room temperature for a long time, and have good room temperature stability; meanwhile, the cryopreservation cost of the mesenchymal stem cells is greatly reduced, and the application is suitable for large-scale clinical application.

The invention discloses a microcolumn chip as well as a preparation method and a use method thereof, and relates to the technical field of biological chips. The microcolumn chip comprises a glass substrate and a PDMS chip arranged on one side of the glass substrate, a cell migration and deformation channel is arranged in the PDMS chip, and a plurality of microcolumns are arranged in the cell migration and deformation channel; the device further comprises a cell injection channel, the downstream of the cell injection channel is communicated with a cell calibration channel, and the cell calibration channel is communicated with the cell migration and deformation channel; the downstream of the Christmas tree-shaped channel is communicated with the cell migration and deformation channel, and the upstream of the Christmas tree-shaped channel is communicated with multiple groups of serpentine channels; according to the microcolumn chip, through the coupling design of the microcolumn channel and the stable chemical gradient, mechanical extrusion and chemotactic signals can be synchronously applied on the basis of simulating the real microenvironment of cells, high bionic simulation of single cell migration and deformation behaviors is achieved, and the limitation that an existing chip can only independently simulate the chemical gradient or mechanical compression is broken through.

The present invention belongs to the field of drug research and development, and relates to an organ microfluidic chip and a drug safety evaluation system. The organ microfluidic chip includes a chip substrate, a cell culture matrix network, a cell injection loading port, an organ chip perfusate output port, and a drug perfusion port; a groove is provided on the chip substrate; the cell culture matrix network is embedded in the groove on the chip substrate; holes are provided on the cell culture matrix network; the cell injection loading port is provided on the top of the cell culture matrix network and is connected to the cell culture matrix network; the cell injection loading port injects cells required by the organ chip into the cell culture matrix network, and the cells eventually gather in the holes; the drug perfusion port is connected to the organ chip perfusate output port through the cell culture matrix network. The present invention provides an organ microfluidic chip and a drug safety evaluation system that can directly and conveniently evaluate the safety or effectiveness of drugs, has a short detection time, and simplifies processing.

The utility model discloses a tube for cell preparation or cell washing, which comprises a tube body, the tube body is used for storing cell washing liquid or cell injection, a sealing plug is fixedly arranged on the inner wall of the tube body and is positioned at a position close to the top end of the tube body, the bottom end of the tube body is fixedly connected with a collecting tube and a filter membrane, and the collecting tube is fixedly connected with the filter membrane. The filter membrane is arranged in the tube body and is used for filtering impurities in the cell suspension mixed solution; the pipe clamp is arranged on the outer wall of the collecting pipe in a sleeving mode and used for closing the collecting pipe. The device has the advantages that preparation or washing operation can be completed in the closed pipe, and the high requirement for the cleanliness of the air environment is lowered to a certain degree; meanwhile, the functions of filtering and metering the liquid volume are also realized. The maximum expected value of the design is that the air cleanliness requirement of a preparation or filtering operation environment can be reduced to a certain extent on the premise of ensuring that the cell product meets the sterile requirement by using the product disclosed by the utility model.

The invention discloses a directional blood flow training composite dilator, and relates to the technical field of medical instruments. The device comprises an expansion bag, one side of the expansion bag is communicated with a water injection connecting pipe, the other end of the water injection connecting pipe is communicated with a water injection kettle, and one side of the expansion bag is provided with a stem cell injection kettle. The hard base plate structure is arranged, the rigid characteristic of the hard base plate can restrain the expansion bag to only expand towards the upper soft tissue direction, directional controllable mechanical stress is formed, a one-way expansion mode promotes new blood vessels to grow in order in the preset direction, the blood vessel density and blood supply quality of the expanded skin flap are remarkably improved, and the expansion effect is good. The design of the uniformly distributed grooves provides an accurate positioning reference for blood flow training, so that a blood supply path can be accurately controlled during follow-up blood flow blocking training through instruments such as an elastic band, finally, an axial flap with a clear vascular pedicle is formed, and the survival rate and the tissue utilization rate of the flap after transplantation are greatly improved.

The invention relates to the technical field of injection equipment, in particular to a mouse cell injection device which comprises an operation table, a plurality of mounting seats are arranged on the upper surface of the operation table, lifting lead screws are vertically and rotatably arranged on the mounting seats, and a mounting frame is arranged on the upper portions of the lifting lead screws; a driving motor used for driving lifting lead screws is arranged on the upper portion of the mounting frame, a lifting block is arranged on a rod body of each lifting lead screw, two sets of sliding rods are arranged between the lifting blocks, a cross beam is arranged on the sliding rods in a sliding mode, and a sliding table used for fixing an injector is arranged on the upper surface of the cross beam in a sliding mode. A positioning device for positioning a blood vessel is arranged on the mounting frame, and the working end of the sliding table faces the positioning device, so that physiological shaking of a person is effectively eliminated, an injection needle can be accurately aligned with a blood vessel positioning point in a three-dimensional space, and the positioning accuracy is improved.