Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
153 results about "Cell injection" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Stem cell injections are used as a form of therapy in regenerative medicine, a branch of medicine that focuses on healing damaged tissues and organs. Stem cells are a special type of cell that can divide and split into nearly any type of cell.
Methods for assisting recovery of damaged brain and spinal cord using arrays of X-ray microplanar beams
ActiveUS7158607B2Promote regenerationMaximum functionX-ray/gamma-ray/particle-irradiation therapyIrradiation devicesX-rayInjury Site
Owner:WASHINGTON UNIV IN SAINT LOUIS +1
Microfabricated cell injector
InactiveUS6846668B1Control flowBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringCell injection
The microfabricated cell injector having an injection wall and a cell injection needle projecting from the wall for piercing cells suspended in a fluid. The needle is held within a housing defined by the internal surfaces of the microfabricated cell injector. The housing has an inlet for suspended cells to enter and an outlet for cells to exit via the cell injection needle. A cell propulsion device is provided for impelling cells towards the needle such that, in use, cells suspended in the fluid are impelled towards the injection wall by the injection needle.
Owner:GARMAN ANDREW
Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes
InactiveCN102920735AIncrease productionEasy quality controlCell dissociation methodsPeptide/protein ingredientsVitamin CSide effect
The invention discloses a mesenchymal stem cell injection, a preparation method and application of the mesenchymal stem cell injection in preparing a medicine for treating diabetes; the mesenchymal stem cells are derived from human umbilical cord and placenta; the mesenchymal stem cell injection consists of components: human mesenchymal stem cells, human albumin, low molecular heparin calcium, compound amino acid, vitamin C of 0.5% and dissolving medium; and the dissolving medium can be a compound electrolyte solution or glucose or normal saline. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparing the medicine for treating the diabetes, the mesenchymal stem cell injection is used for repairing injured islet beta cells, and the blood sugar is reduced by secretion of the endogenous insulin, therefore, a purpose of foundational treating the diabetes is achieved. A disease course of the diabetes can be reversed, patients are helped in escaping out of inconvenience and toxic and side effects of taking the endogenous drug and injecting the insulin as well as serious complications caused by poor control of the blood sugar; and the 1-type and 2-type diabetes are treated thoroughly.
Owner:青岛奥克生物开发有限公司
Three-dimensional high-flux medicaments sifting chip and manufacture method thereof
InactiveCN101245311ALow costShort cycleBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringPolydimethyl siloxane
The invention discloses a three-dimensional high-flux drug screening PDMS-glass chip and the preparation method thereof, which makes uses of micro-electro processing and micro-plastic film technologies to produce the three-dimensional PDMS-glass chip with a two-layer micro-channel structure. A cell injection and waste liquid output channel of a first layer of PDMS chip is bonded with another layer of polydimethyl siloxane chip together, a drug concentration gradient channel and a quantitative cell culture chamber are arranged on the other layer of PDMS chip, the quantitative cell culture chamber is connected with an outlet channel of the drug concentration gradient channel, the quantitative cell culture chamber is respectively connected with a cell sample injection hole drilling position and a waste liquid outlet hole drilling position by the channel; the three-dimensional high-throughput drug screening PDMS-glass chip can overcome the shortcomings of heavy workload, great reagent and drug consumption, long screening period and high development cost of the traditional drug screening technology. The three-dimensional high-flux drug screening PDMS-glass chip can realize miniaturization of the chip, high flux, low cost, small reagent consumption, accurate and reliable test data and the simple design requirements of the operational process in the drug screening application on cell level.
Owner:WUHAN UNIV +1
Mesenchymal stem cell cryopreserved liquid and injection liquid
The invention discloses a mesenchymal stem cell cryopreserved liquid which comprises the following ingredients in percentage by volume: 25%-75% of plasmalyte-A, 10%-35% of an 18AA5% compound amino acid injection liquid, 1%-50% of 20% human serum albumin and 5%-20% of DMSO (dimethylsulfoxide). The invention further provides a mesenchymal stem cell injection liquid and a preparation method thereof. According to the mesenchymal stem cell cryopreserved liquid, the defects of a conventional mesenchymal stem cell cryopreserved liquid can be overcome, not only can the motility rate of the mesenchymal stem cell be maintained for a long time, but also the direct clinical infusion can be performed, and accordingly, the mesenchymal stem cell cryopreserved liquid has a good application prospect and economic benefits.
Owner:SICHUAN NEO LIFE STEM CELL BIOTECH
Cell microinjection instrument
InactiveCN1763170AEasy to operateIncrease success rateBioreactor/fermenter combinationsBiological substance pretreatmentsSuccessful injectionCell membrane
The microscopic cell injecting instrument includes cell conveyer, cell injector, cell holder and digital control and drive system. In cell injection, cell is conveyed automatically with the cell conveyer and in microfluid conveying mode to the cell injector, and the outlet of the cell holder. During cell injection, all the cell conveyance, holding, cell membrane puncture with the injection needle, intracellular medicine injection, the withdrawn of injection needle, cell release and other microscopic operation steps are computer digital controlled in the same computer operating interface. The present invention has simple operation, high successful injection rate, and automatic separation and counting of injected and un-injected cells.
Owner:章维一 +2
MSC (mesenchymal stem cell) injection as well as preparation and application thereof
InactiveCN104857022AImprove survival rateAvoid gatheringPharmaceutical delivery mechanismUnknown materialsClinical gradeHydroxyethyl starch
The invention relates to the field of biology, in particular to MSC (mesenchymal stem cell) injection as well as a preparation and an application thereof. The injection comprises MSCs and a cell cryopreservation solution, wherein the cryopreservation solution comprises components in percentage by volume as follows: 25%-70% of an electrolyte balance solution, 5%-20% of clinical-grade DMSO (dimethyl sulfoxide), 1%-50% of 20% human serum albumin, 1%-10% of hydroxyethyl starch 130 / 0.4 and 5%-20% of triphosadenine-disodium magnesium chloride freeze-drying powder. The injection is free of animal serum, has clear ingredients and good cell cryopreservation effect and is safe and controllable, long-term storage and long-distance transport are facilitated, the survival rate of cells after recovery is higher than 95%, the vitality is high, and the injection can effectively relieve injury and inflammation symptoms of lesion tissue of lungs and promote tissue regeneration of the lungs, so that acute lung injury can be fundamentally and comprehensively treated.
Owner:北京青藤谷禧干细胞科技研究院有限公司
Micro-fluidic chip and method for research on oriented movement of cell in three-dimensional medium
InactiveCN101748059ADetection of mobilityReduce dosageMicrobiological testing/measurementTissue cultureThree-dimensional spaceConcentration gradient
The invention provides a micro-fluidic chip and a method for research on oriented movement of cell in three-dimensional medium. The micro-fluidic chip comprises a cell injection port, a cell culture pool, two parallel perfusion channels and a waste liquid pool; the lower ends of the perfusion channels are connected with the waste liquid pool, the cell culture pool is connected with the two parallel perfusion channels, the upper end of the waste liquid pool is connected with the cell injection port; and the micro-fluidic chip is used to provide three-dimensional space support for growth and migration of the cell, simultaneously stable concentration gradient is formed in the three-dimensional space, and with the inducement of the chemoattractant, the cell moves in orientation in the three-dimensional medium. The invention has the advantages of simple and fast operation, low sample use level and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Leak testing device and method for battery case
InactiveCN102297752AHigh sensitivityGuaranteed pass rateMeasurement of fluid loss/gain rateEngineeringLeak testing
The invention discloses a leak detection device and a leakage detection method for a battery shell, belonging to the field of power battery development. The leak detection equipment includes a plugging head and a vacuum system, the liquid injection port of the battery to be tested is connected to the plugging head, the vacuum system includes a pipeline, the plugging head is connected to the pipeline, the There is a vacuum pressure gauge on the pipeline. The present invention improves the sensitivity of battery casing leak detection by using a vacuum pressure gauge to test pressure changes, so that small leaks can also be detected, ensuring the pass rate of the battery casing, and at the same time has the advantages of simple operation and easy observation of detection results , Leak detection time is short and so on.
Owner:CHERY AUTOMOBILE CO LTD
Umbilical cord mesenchymal stem cell injection as well as preparation method and application thereof
InactiveCN104873542AImprove the quality of lifeReduce releaseDigestive systemPharmaceutical delivery mechanismHydroxyethyl starchClinical grade
The invention provides umbilical cord mesenchymal stem cell injection as well as a preparation method and application thereof. The umbilical cord mesenchymal stem cell injection comprises mesenchymal stem cells and a frozen stock solution, wherein the frozen stock solution comprises the following components: a balanced electrolyte solution with the volume percentage of 25-70 percent, hydroxyethyl starch 130 / 0.4 with the mass / volume percentage (g / ml) of 1-10 percent, triphosadenine disodium magnesium chloride with the volume percentage of 5-20 percent, clinical-grade DMSO with the volume percentage of 5-20% and 20% human albumin with the volume percentage of 1-50 percent. The prepared umbilical cord mesenchymal stem cell injection is free of animal serum, safe, high in cell viability after cryopreservation resuscitation, can be used for treating inflammatory bowel disease, has a good effect and has no toxic or side effects.
Owner:北京青藤谷禧干细胞科技研究院有限公司
Mixing stem cell injection and preparation method thereof
ActiveCN103330720AImprove securityProliferation effect is goodAntinoxious agentsMammal material medical ingredientsProgenitorMesenchymal stem cell
The invention discloses a mixing stem cell injection and a preparation method thereof, and belongs to the field of cell preparation agents. The injection of the invention is mixture of mesenchymal stem cells from umbilical cord source and hematopoietic stem / progenitor cells from the umbilical cord blood source; the method of the mixing stem cell injection comprises the following steps: CD34+ hematopoietic stem cells are separated from the fresh umbilical cord blood and purified, and are subjected to mixed culture and amplification together with the mesenchymal stem cells from the umbilical cord source, during the amplification, the culture system is culture medium including AB umbilical cord blood plasma the volume fraction of which is 15 percent and the cell factor, 6*104 mesenchymal stem cells and CD34+ hematopoietic stem cells with the concentration of 5*104 / ml are inoculated into a culture flask and are cultured for about 10 days, and two kinds of cells are obtained at the same time, so that the mixed stem cell injection including 5*105 / ml of hematopoietic stem / progenitor cells and 2*105 / ml of mesenchymal stem cells and containing normal saline with the volume fraction of AB umbilical cord blood plasma as 15 percent is obtained.
Owner:广州市天河诺亚生物工程有限公司
Umbilical cord mesenchymal stem cell injection and preparation method and application thereof
InactiveCN104922059ANo immune rejectionNon-tumorigenicPharmaceutical delivery mechanismUnknown materialsHydroxyethyl starchFiltration
The invention relates to umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. The injection comprises umbilical cord mesenchymal stem cells and mixed solution; the mixed solution comprises balanced electrolyte solution, hydroxyethyl starch, adenosine disodium triphosphate-magnesium chloride, dimethyl sulfoxide and human serum albumin. The preparation method comprises the following steps: (1) fully and evenly mixing the balanced electrolyte solution, the hydroxyethyl starch and the human serum albumin, and performing filtration sterilization; (2) adding the umbilical cord mesenchymal stem cells; (3) adding the dimethyl sulfoxide; (4) performing split charging and frozen preservation to finish preparation; the invention also provides the application of the injection in the aspect of drugs for treating chronic ischemic heart diseases. The injection disclosed by the invention has a very good resuscitation effect after the frozen preservation, can be directly injected after being unfrozen and resuscitated, and has a remarkable effect on treatment on the chronic ischemic heart diseases.
Owner:北京青藤谷禧干细胞科技研究院有限公司
Mesenchymal stem cell injection and preparation method thereof as well as application in preparation of medicine for treating ulcerative colitis
InactiveCN102920734AIncrease productionEasy quality controlDigestive systemMammal material medical ingredientsSide effectUlcerative colitis
The invention discloses a mesenchymal stem cell injection and a preparation method of the mesenchymal stem cell injection and an application in preparation of a medicine for treating ulcerative colitis, wherein the mesenchymal stem cell injection consists of the following components: 2*105-1*107 pieces / ml of mesenchymal stem cells, 5-10% of dimethyl sulfoxide (DMSO) by volume, 1-6% of human albumin by mass-to-volume ratio, and 1% of low molecular dextran and compound electrolyte solution. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparation of the medicine for treating ulcerative colitis, the mesenchymal stem cell injection is used for correcting immune dysfunction of a patient of ulcerative colitis, and preventing the autoimmune dysfunction from continuously damaging the intestinal tract; meanwhile, many cell growth factors and repair factors are secreted, to promote pathologically changed intestinal ulcer to heal, therefore, the purpose of foundational treating the ulcerative colitis is achieved. The disease course of the ulcerative colitis can be reversed, the patient can be helped in escaping out of influence of stomachache and diarrhea on the life as well as toxic and side effects of the medicine, so as to treat the ulcerative colitis thoroughly.
Owner:青岛奥克生物开发有限公司
Cell injection for treating bone injury and preparation method thereof
InactiveCN102232970AReduce the impactSimple and fast extraction procedureSkeletal disorderUnknown materialsCell injectionTemperature sensitive
The invention relates to a cell injection for treating bone injury and a preparation method thereof, belonging to the field of tissue engineering. The injection is a compound formed by mesenchymal stem cells from autologous bone marrow, temperature sensitive gel and effective trophic factors separated and extracted from autoblood. The preparation method comprises the steps of: after extracting the bone marrow of a patient, separating and extracting the mesenchymal stem cells from the bone marrow, adding no animal serum in the amplification culture process, and mixing 1*(105-108) cells with 1-5ml of biological scaffold material to form the injectable compound. The cell injection for treating bone injury is mainly used for treating bone injuries including osteoarthritis, femoral head necrosis and delayed union and disunion of bone fracture, is convenient to use, has notable effect without immunological rejection, and can alleviate the great pain of patients caused by bone injury.
Owner:董运海
Mesenchymal stem cell injection, as well as preparation method and application in preparing medicament for treating children dilated cardiomyopathy
InactiveCN102908364AIncrease productionEasy quality controlPeptide/protein ingredientsMammal material medical ingredientsClinical gradeLife quality
The invention provides mesenchymal stem cell injection, as well as a preparation method and application in preparing a medicament for treating children dilated cardiomyopathy. The mesenchymal stem cell injection comprises 2*10<5>-1*10<7> mesenchymal stem cells in every milliliter, clinical grade DMSO (dimethylsulfoxide) with volume ratio of 5 to 8 percent, human albumin with mass volume ratio of 1 to 6 percent, and multi-electrolyte liquid. The mesenchymal stem cell injection is prepared from placenta and umbilical cord, has the advantages of being high in yield, and being easy to realize industrialization, the preparation system quality is easy to control; the injection can be directly frozen through program-controlled freezing procedure and directly used in clinical injection after being recovered, and is safe and reliable. According to the injection, the dilated cardial structure can be changed, the function of heart can be recovered, the water supply state of each tissue in the whole body can be improved, the living quality of a patient can be improved, and the dilated cardiomyopathy can be treated fundamentally, so that the patient can get rid of serious complications caused by massive medicine administration, living action limitation and poor disease control.
Owner:青岛奥克生物开发有限公司
Umbilical cord mesenchymal stem cell injection with anti-aging function and preparation method thereof
InactiveCN105920042AWith amplificationDirected differentiationCell dissociation methodsCulture processTissue repairSelf recovery
The invention provides an umbilical cord mesenchymal stem cell injection with an anti-aging function and a preparation method thereof. The injection comprises the following components: umbilical cord mesenchymal stem cell (5*10<5> to 10*10<5> / mL), 2 wt% of human albumin, and 98 wt% of compound electrolyte solution. The produced umbilical cord mesenchymal stem cells can rapidly repair and replace damaged cells and tissues, and have the characteristics of amplification and directional cell differentiation; and after the stem cells receive the signals from damaged parts, the stem cells will move to the damage parts, amplify in the damage parts, carry out induced differentiation, promote the self recovery function of stem cells, rapidly repair and replace damaged cells and tissues, rapidly secrete cell factors, growth factors, and tissue related factors in time, thus improve the blood circulation, enhance the metabolism functions, improve the tissue repairing function and immunity, and finally achieve the anti-aging goal.
Owner:SHANGHAI HUAYAN MEDICINE TECH CO LTD
High-throughput automated cellular injection system and method
ActiveUS20080077329A1Easy to adaptReduce throughputBiological testingSpecial data processing applicationsHigh fluxGenetic Materials
An automated cell injection system and method are described, which can perform automatic, reliable, and high-throughput cell injection of foreign genetic materials, proteins, and other compounds. The system and method overcome the problems inherent in traditional manual injection that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for zebrafish embryo injection but can be readily extended to other biological injection applications such as mouse embryo, drosophila embryo, and C. elegans injections, capable of facilitating high-throughput genetic research at both academic and industry levels. A novel vacuum based cell-holding device is also provided.
Owner:SUZHOU BOUNDLESS MEDICAL TECH CO LTD
Method for high-efficiency establishment of genetically modified animal model through haploid stem cells
The invention discloses a method for high-efficiency establishment of a genetically modified animal model through haploid stem cells. Specifically, the method for high-efficiency establishment of the genetically modified animal model through haploid stem cells comprises the following steps: a) knocking out the H19 gene of male haploid embryonic stem cells through homologous recombination; b) screening and identifying the male haploid embryonic stem cells in which the H19 gene is correctly knocked out; and c) injecting the male haploid embryonic stem cells in which the H19 gene is correctly knocked out into cytoplasm of oocyte so as to produce a semi-cloned genetically modified animal. The method provided by the invention simulates the functions of sperms by establishing an H19 gene-knocked-out male haploid embryonic stem cell system so as to highly efficiently generate fertile progeny, which can then be used for implementation of novel genetic manipulation and establishment of corresponding genetically modified animal models.
Owner:THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
Dielectric cytometric apparatus and dielectric-cytometric cell sorting method
Disclosed herein is a dielectric cytometric apparatus capable of analyzing cells and sorting the cells without adopting an optical analysis method and provides a dielectric cytometric cell sorting method for the apparatus. A stenosis channel allowing a single cell to flow is created on a cell injection section of a micro flow channel device used as a portion of the dielectric cytometric apparatus. A pair of measurement electrodes is created on the stenosis channel and an analyzer connected to the measurement electrodes measures the complex dielectric constant of for every cell passing through the stenosis channel. A electric-field application section provided on the downstream side of the stenosis channel applies an electric field for changing the flow of the cells inside a flow channel on the basis of information on the measured complex dielectric constants so that the cells can be sorted by making use of branch channels.
Owner:SONY CORP
Method for accurate in vivo delivery of a therapeutic agent to a target area of an organ
InactiveUS20070167700A1Precise deliveryAccurate in vivo deliverySurgical needlesSurgical navigation systemsCell injection3d image
In a method for accurately delivering a therapeutic agent to a target area of an organ of a living subject, such as for injecting stem cells into the myocardium of the heart, a 3D image, in which the target area and a delivery path thereto are visible, is obtained prior to delivery of the therapeutic agent. The 3D image is displayed, and a catheter is introduced into the subject and a real time positional indication of the catheter in the subject is obtained and incorporated into the displayed image, providing visual support for guiding the catheter to the target area. When the catheter is at the target area, the therapeutic agent is injected into the target area via the catheter. The distribution of the injected therapeutic agent relative to the target area is then monitored in the displayed image.
Owner:SIEMENS AG
Stem cell culture solution and injection
ActiveCN106754668AKeep aliveHealth recoveryCulture processPharmaceutical delivery mechanismDipeptideHuman albumin
A stem cell culture solution is prepared from raw materials as follows: 10% of a compound amino acid injection, 1% of water-soluble vitamins for injection, 0.5% of glutamine dipeptide, 10% of human albumin, 10% of a hydrolyzed protein injection, 0.5% of a microelement injection and 68% of a plasmalyte-A solution, wherein the pH value is 6.8-7.0, and the osmotic pressure is 310 mOsm / kg. The stem cell culture solution is used for culturing mesenchymal stem cells, and a mesenchymal stem cell culture supernatant is collected; the collected culture supernatant is filtered by a filter membrane, and filtrate is collected and is the stem cell injection. A decellularized stem cell secretion factor is used for intravenous injection, so that the dispute of stem cells in the aspect of ethic is effectively avoided; the stem cell culture solution is prepared from clinical raw materials, is safe and reliable, provides nutrition for the living environment of the stem cells, can effectively promote the stem cells to secrete a large quantity of cell factors and keeps the activity of the stem cells; through cooperation of a nutritional supplement formula and the stem cell secretion factor, the sub-health state of a body can be effectively relieved, and the body health is regained.
Owner:沈阳细胞治疗工程技术研发中心有限公司
Apparatus for detecting cell chemotaxis
InactiveUS20060121600A1Improve automationBioreactor/fermenter combinationsBiological substance pretreatmentsDiffusionConcentration gradient
The present invention relates to an improvement in an apparatus for detecting chemotaxis of cells. It aims at providing a structure for detecting chemotaxis of cells at an elevated accuracy with the use of a microquantity of cells. That is to say, an object of the present invention is to provide an apparatus for detecting chemotaxis of cells by which cell injection and position control can be easily carried out while ensuring the prevention of unexpected migration of the cells definitely positioned in a well or the injected sample so that a stable concentration gradient due to the diffusion of the specimen can be maintained and which ensures further automated operation and controlling. Namely, an apparatus for detecting chemotaxis of cells with a structure wherein two wells are connected to each other via a channel having resistance to the passage of cells and each well has an opening for injecting cells or a specimen, characterized by having (1) a means of transporting a liquid and a means of stopping the transportation after the injection or the aspiration discharge of the liquid and (2) a means of sealing the opening(s) in one or both of the cell-injection side and the specimen-injection side.
Owner:ECI
In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel
InactiveCN103497931ASimple methodGood repeatabilityTumor/cancer cellsAnimal husbandryCancer cellMalignancy
The invention discloses application research of an in-vivo tumor engineering human oophoroma tumor model established on the basis of an injectable collagen aquogel composite human oophoroma cells in a noninvasive subcutaneous injection form to a nude mouse. The injectable collagen aquogel stent used as a substrate material is compounded with human oophoroma cell SKOV3 to noninvasively establish the in-vivo tumor engineering human oophoroma tumor model at the nude mouse subcutaneous tissues; and compared with the traditional human oophoroma tumor model established in an independent cell injection mode under the condition of no stent material, the detection on the tumor model indicates that the malignancy of the human oophoroma tumor established on the basis of the injectable collagen aquogel is higher, and is more approximate to that of the human oophoroma tumor, and the in-vivo tumor model established in such a mode has smaller trauma possibility. The tumor model established by the method has higher tumor formation rate (up to 95%), which is about 15% higher than the traditional mode, thereby providing a more reliable tumor model for further research.
Owner:GUANGXI MEDICAL UNIVERSITY
Stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof
The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells / 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells / one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells / one time / one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.
Owner:WUHAN HAMILTON BIOTECH
Cell microinjection system with force feedback
ActiveUS20190292567A1Low costEasy to installAnimal reproductionProgramme-controlled manipulatorElectricityEmbryo
A novel piezo-driven cell injection system with force feedback overcomes the unsatisfied force interaction between the pipette needle and embryos in conventional position control. By integrating semiconductor strain-gage sensors for detecting the cell penetration force and the micropipette relative position in real time, the developed cell microinjection system features high operation speed, confident success rate, and high survival rate. The effectiveness of the developed cell injection system is experimentally verified by penetrating zebrafish embryos. The injection of 100 embryos are conducted with separate position control and force control. Results indicate that the force control enables a survival rate of 86%, which is higher than the survival rate of 82% produced by the position control in the same control environment. The experimental results quantitatively demonstrate the superiority of force control over conventional position control for the first time.
Owner:UNIVERSITY OF MACAU
Multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously
ActiveCN105838603AShorten the timeReduce experimental errorBioreactor/fermenter combinationsBiological substance pretreatmentsCancer cellTumor cells
The invention provides a multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously and belongs to the technical field of microfluidics. By means of the multifunctional integrated microfluidic chip, the experiment of conducting online screening of multiple types of cancer cells simultaneously can be completed, and 16 types of cancer cells can be inoculated; online screening of multiple types of medicine simultaneously can be achieved, and online screening of eight types of cancer-resistant medicine simultaneously can be completed. The multifunctional integrated microfluidic chip comprises three layers, namely a first PDMS film layer, a second PDMS film layer and a glass bottom layer, and the three parts are bonded by irreversible plasms from top to bottom in sequence to form a composite integrated structure. Four modules of the same structure and a waste liquid opening are integrated into a chip body, four columns of cell culture channels, a fluid channel, a cell injection opening, two medicine injection openings and six micro valves are integrated into each module, a bent pipe communicated with the cell culture channels is arranged below each column of cell culture channels, and the top end of each bent pipe is provided with an outlet.
Owner:浙江弘瑞医疗科技有限公司
Mesenchymal stem cell injection as well as preparation method and application thereof
InactiveCN106177918AEasy to get materialsNo ethical issuesOrganic active ingredientsNervous disorderVitamin CMesenchymal stem cell
The invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell injection as well as a preparation method and application thereof. The mesenchymal stem cell injection comprises mesenchymal stem cells, human serum albumins, low-molecular weight heparin calcium, vitamin C, lentinan, dimethyl sulfoxide and a menstruum. The mesenchymal stem cell injection provided by the invention can effectively maintain the activity of the stem cells, can increase the content of striatal dopamine and can effectively treat the Parkinson's disease.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Micro-fluidic chip and method for studying effect of fluid shearing force on cell with the micro-fluidic chip
InactiveCN102816695AMicrobiological testing/measurementTissue/virus culture apparatusFluid shearEngineering
The invention provides a micro-fluidic chip and a method for studying an effect of fluid shearing force on a cell with the micro-fluidic chip. The micro-fluidic chip is composed of four cell culture inserts and four fluid passages, wherein the four cell culture inserts have the same size and are parallel mutually; a cell culture insert a and a cell culture insert b share one cell injection port and one cell waste reservoir; a cell culture insert c and a cell culture insert d share one cell injection port and one cell waste reservoir; the upper ends of the four cell culture inserts are separately connected with the four fluid passages; the lower ends of the four cell culture inserts are connected with the same waste reservoir; and the four fluid passages collectively start from a nutrient solution injection port, with the tail ends being connected with the four cell culture inserts respectively. The micro-fluidic chip can be used for studying the effect of the fluid shearing force on the cell.
Owner:DALIAN MEDICAL UNIVERSITY
DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid
PendingCN112772637ASo as not to damageReduce adverse effectsDead animal preservationSodium Chloride InjectionMesenchymal stem cell
The invention discloses a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid, which is characterized by being prepared from the following raw materials in parts by volume: 50 to 60 parts of compound electrolyte injection, 20 to 40 parts of dextran 40 glucose injection, 1 to 10 parts of sodium chloride injection, 1 to 10 parts of glucose injection, 30 to 50 parts of human serum albumin, and 1 to 10 parts of a mesenchymal stem cell serum-free medium. The cryopreservation liquid does not contain DMOS or serum, so that the risk of clinical use is reduced, the influence of the uncertainty of serum components and the instability of serum culture on the normal induced differentiation function of the mesenchymal stem cells is avoided, and the cryopreservation liquid enables the human umbilical cord mesenchymal stem cells to keep a good cryopreservation effect, and the human umbilical cord mesenchymal stem cells have high survival rate after cryopreservation and resuscitation. In addition, the cells cryopreserved by the cryopreservation liquid can be directly diluted and then applied clinically, components of the cryopreservation liquid do not need to be removed through centrifugation, and the cryopreservation liquid can be used as an auxiliary material and directly applied to clinical administration, so that the cryopreservation liquid is more convenient to use.
Owner:朱灏
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com