Cell micro-carrier matrix and application thereof

Abstract

The invention provides a cell micro-carrier matrix, which is prepared from the following raw materials: methacrylated gelatin and oyster shell powder, and the mass ratio of the methacrylated gelatin to the oyster shell powder is (1-10): (0.5-2). The methacrylated gelatin is high in water content, similar to an extracellular matrix in component and good in surface activity, so that the methacrylated gelatin can be well compatible with cells and active factors, the cells are wrapped in the cell micro-carrier matrix containing the methacrylated gelatin as an effective component, a three-dimensional supporting effect can be provided for the cells, and the cells can be prevented from being damaged in the injection and transplantation processes. The methacrylated gelatin and the oyster shell powder are compounded for use, so that the swelling rate of the cell micro-carrier can be effectively reduced, and the cell micro-carrier has more excellent mechanical strength.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell microcarrier matrix and its application. Background technique [0002] Cell therapy is a method that uses active cells to repair tissues and organs. The cells used in cell therapy mainly include stem cells, mature functional cells, somatic cells, heterogeneous cells and transdifferentiated cells. According to the source of cells, the cells used in cell therapy can be divided into two types, one is autologous cells , derived from the patient's own body; the other is allogeneic cells, derived from a donor. Regardless of the source of cells, in the process of application, they generally need to go through the process of in vivo extraction, in vitro activation and culture, and in vivo transfusion. Cell injection is a commonly used method to inject cells into the patient's body, but there are some defects in the process of cell injection; firstly, the loss of cells is...

AI Technical Summary

Problems solved by technology

Cell injection is a commonly used method of injecting cells into the patient's body, but there are some defects in the process of cell injection; firstly, the loss of cells is serious. During the process of injecting target cells into the human body, the cell retention rate is lower due to tissue mechanical force. Low, the effective cells that finally reach the damaged site are insufficient; secondly, the survival rate of cells after transplantation is not high, which not only wastes the source of cells, but also consumes a large amount of cells, and directly affects the effective effect of the cells injected into the body

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