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Retention Method of Enhanced Cell Microcarrier Perfusion Culture

A cell microcarrier and perfusion culture technology, which is applied in the interception field of cell microcarrier perfusion culture, can solve the problems of low solid-liquid separation efficiency, loss of microcarriers, and difficult control of liquid level, etc., and achieves small cell damage, increased perfusion volume, The effect of improving retention efficiency

Active Publication Date: 2021-04-30
SHANDONG YIDU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the volume of perfusion required per day is large, it is difficult to meet the requirements with a settling column, which will cause the loss of microcarriers, low solid-liquid separation efficiency, and the liquid level is not easy to control

Method used

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  • Retention Method of Enhanced Cell Microcarrier Perfusion Culture
  • Retention Method of Enhanced Cell Microcarrier Perfusion Culture

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Embodiment Construction

[0019] The present invention will be further described below with reference to the accompanying drawings.

[0020] Enhanced cell microcarrier perfusion culture retention device and method, the device comprises a sedimentation column body 2, a sedimentation cavity 4, a layered funnel 3, a medium-microcarrier inlet 6, a medium outlet 1, a microcarrier return port 5, Observation window 7, sedimentation column 8, bioreactor 9, peristaltic pump 10, the device body is provided with a layered funnel 3, the bottom of the layered funnel 3 is a sedimentation cavity 4, and a culture medium-microcarrier is opened on the sedimentation chamber 4 The inlet 6, the bottom of the sedimentation chamber 4 is connected with a connecting pipe, the bottom of the connecting pipe is the medium-microcarrier return port 5, the top of the layered funnel 3 is the medium outlet 1, and the side layered funnel 3 of the device body is provided with an observation window. 7.

[0021] The medium-microcarrier i...

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PUM

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Abstract

The invention provides an enhanced interception device and method for perfusion culture of cells with microcarriers, wherein a layered funnel is arranged inside the device body, and the bottom of the layered funnel is a settling chamber, and a culture medium-microcarrier inlet is opened on the settling chamber, and the sedimentation The bottom of the cavity is connected with a connecting pipe, the bottom of the connecting pipe is the medium-microcarrier return port, the top of the layered funnel is the outlet of the medium, and there is an observation window at the layered funnel on one side of the device body; the advantage is that the present invention has solid-liquid settlement The function of separating and controlling the liquid level can improve the perfusion volume and interception efficiency of cell microcarrier culture, solve the problem of microcarrier loss due to not easy sedimentation when the liquid feeding speed is too high, and cause less damage to cells during the process of microcarrier retention.

Description

technical field [0001] The invention relates to the field of cell microcarrier perfusion culture, in particular to an enhanced retention method for cell microcarrier perfusion culture. Background technique [0002] The use of perfusion culture systems instead of traditional batch or batch-supplemented production processes can greatly improve cell density and yield of target products, and is increasingly used in the biopharmaceutical industry. For efficient perfusion culture, microcarriers need to be entrapped. The retention mode of microcarriers can be divided into internal retention and external retention. Internal interception mainly includes hollow fiber filtration, rotary filtration and other technologies, and external interception methods include external filtration, centrifugation and sedimentation technologies, but in practical applications, these devices have limited interception efficiency, or the cost is too high, or the use time is not long, and Can not meet the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/36C12N5/00
Inventor 庄长鹰
Owner SHANDONG YIDU BIOTECH
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