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Cell microcapsule, cell microcapsule loaded with anticancer drug, preparation method and application thereof

An anti-cancer drug and cell technology, applied in the field of biology and medicine, can solve the problems of lack of biological activity and restraint

Active Publication Date: 2020-10-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, liposomes used clinically have a particle size of about 100nm and contain lipid components such as phospholipids and cholesterol. However, the lack of biological activity as an exogenous substance restricts its further development as a drug carrier for precision therapy.

Method used

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  • Cell microcapsule, cell microcapsule loaded with anticancer drug, preparation method and application thereof
  • Cell microcapsule, cell microcapsule loaded with anticancer drug, preparation method and application thereof
  • Cell microcapsule, cell microcapsule loaded with anticancer drug, preparation method and application thereof

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preparation example Construction

[0060] One embodiment of the present invention provides a method for preparing cell microcapsules, comprising the following steps:

[0061] (1) Cultivate immune cells and take cell supernatant;

[0062] (2) centrifuging the cell supernatant described in step (1) to remove cells and cell debris, and taking the supernatant;

[0063] (3) centrifuging the supernatant described in step (2) to remove apoptotic bodies, and taking the supernatant;

[0064] (4) centrifuging the supernatant described in step (3), and taking the precipitate to obtain final product;

[0065] The centrifugation conditions described in step (3) include: the centrifugal force is 2000-3000g, and the centrifugation time is 40-100min.

[0066] The preparation method is very simple to operate and can obtain cell microcapsules with good biological activity, complete structure and high purity.

[0067] In the preparation method, the centrifugation condition is further preferably a differential centrifugation me...

Embodiment 1

[0085] Example 1 Cell microcapsules derived from macrophages loaded with doxorubicin

[0086] 1. Preparation of fat-soluble doxorubicin

[0087] 1. Weigh 20mg of doxorubicin hydrochloride into a 100ml round bottom flask, add 20ml of dichloromethane to fully dissolve.

[0088] 2. Add 14.3ml of triethylamine according to the molar ratio of 1:3, and stir overnight at 25°C.

[0089] 3. After 24 hours, collect the product and place it in a dialysis bag for dialysis until the dialysate is clear and colorless.

[0090] 4. Collect the material in the dialysis bag and freeze-dry it to obtain dark red doxorubicin powder.

[0091] 5. Dissolve 543.52 μg of doxorubicin powder in 1 mL of DMSO to prepare a doxorubicin solution with a final concentration of 1 mM.

[0092] 2. Preparation of cell microcapsules

[0093] In 95% air and 5% CO 2 In a cell culture incubator, in a sterile and non-toxic environment, at a culture temperature of 35°C-37°C, continuously culture macrophages (RAW264.7...

Embodiment 2

[0100] Example 2 Cell microcapsules derived from macrophages loaded with paclitaxel

[0101] 1. Configuration of paclitaxel stock solution

[0102] Weigh 853.91 μg of paclitaxel powder and dissolve it in 1 mL of DMSO to prepare a paclitaxel solution with a final concentration of 1 mM.

[0103] 2. Preparation of cell microcapsules

[0104] In 95% air and 5% CO 2 In a cell culture incubator, in a sterile and non-toxic environment, at a culture temperature of 35°C-37°C, continuously culture macrophages (RAW264.7) in a complete medium containing 10% serum for more than 2 days, collect the cell supernatant for micro Capsule extraction and purification, the experimental steps are as follows:

[0105]1. Remove cells and cell debris: Centrifuge the above cell supernatant at 500g for 10 minutes, discard the precipitate, and take the supernatant;

[0106] 2. Remove apoptotic bodies: centrifuge the supernatant at 2500 g for 1 hour in step (1), discard the precipitate, and take the su...

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Abstract

The invention relates to a cell microcapsule and a preparation method thereof, a cell microcapsule loaded with cancer resisting medicines, and a preparation method and application of the cell microcapsule loaded with cancer resisting medicines. The preparation method of the cell microcapsule comprises the following steps of (1) culturing immunocyte, and taking cell supernatant; (2) centrifuging the cell supernatant obtained in the step (1) to remove cells and cell fragments, and taking supernatant; (3) centrifuging the supernatant obtained in the step (2) to remove apoptotic bodies, and takingsupernatant, wherein the centrifuging condition includes that the centrifugal force is 2000-3000g, and the centrifuging time is 40-100min; and (4) centrifuging the supernatant obtained in the step (3), and taking precipitate. The preparation method is simple to operate, the cell microcapsule which is good in bioactivity, complete in structure, high in purity and uniform in particle diameter can be obtained; the obtained cell microcapsule can load liposoluble cancer resisting medicines through simple incubation, and the cell microcapsule is good in effects, good in targeting properties and lowin toxic and side effects when being used for preventing or treating cancer diseases.

Description

technical field [0001] The invention relates to the technical fields of biology and medicine, in particular to a cell microcapsule, a cell microcapsule loaded with anticancer drugs, a preparation method and an application thereof. Background technique [0002] According to the World Health Organization, tumors, or cancer, are the second leading cause of death in the world, accounting for nearly one in six deaths globally. Liposome dosage forms are often used in clinical cancer treatment because they can reduce the toxic and side effects of drugs in the body. At present, liposomes used clinically have a particle size of about 100nm and contain lipid components such as phospholipids and cholesterol. However, due to their lack of biological activity as exogenous substances, their further development as a drug carrier for precision therapy is hampered. The vesicles secreted by various immune cells in the body's circulatory system have a variety of physiological functions. Cros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/46A61K31/704A61K31/337A61K31/175A61P35/00
CPCA61K9/5063A61K31/175A61K31/337A61K31/704A61P35/00
Inventor 冯敏郭羚
Owner SUN YAT SEN UNIV
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