9 results about "Apoptotic body" patented technology

The invention discloses a phosphatidylserine modified gliadin nanoparticle as well as a preparation method and application thereof. The phosphatidylserine modified gliadin nanoparticle is of a core-shell structure, gliadin is entrapped by polylactic acid-glycolic acid copolymer to serve as a core, and a lipid composite membrane formed by phosphatidylserine and DSPE-PEG2000 is coated outside the nanoparticle. The gliadin-loaded nanoparticles modified by phosphatidylserine are used for simulating membrane signal characteristics of apoptotic bodies, enhancing recognition and uptake of the nanoparticles by macrophages and inducing tolerance phenotypic transformation of the nanoparticles, so that immune tolerance of celiac disease patients to gliadin is recovered, and the gliadin-loaded nanoparticles can be used for treating celiac disease patients. On the basis of simplifying the preparation process, the problems of unstable tolerance effect and lack of metabolic regulation in the existing nano immunotherapy are further solved. The nano-particles prepared by the technical route have good particle size distribution and stability, can effectively deliver antigens and induce immune tolerance, and are a novel convertible celiac disease treatment nano-preparation.

The invention relates to the technical field of biological medicine, in particular to an efficient macrophage targeting vesicle system as well as a preparation method and application thereof. The vesicle system is a chimeric vesicle (mHEs), and is prepared from extracellular vesicles (M2-EVs) derived from M2 type macrophages and apoptotic small bodies (MSC-ABs) derived from bone marrow mesenchymal stem cells. The vesicle system provided by the invention can realize specific recognition and functional regulation of macrophages, thereby playing an efficient therapeutic role in pathological processes such as tissue inflammation repair, myocardial infarction, ischemia-reperfusion injury and immune metabolism remodeling.

The invention relates to the technical field of biological tissue engineering, in particular to a young mesenchymal stem cell-derived apoptotic body as well as a preparation method and application thereof. The apoptotic body is formed by inducing apoptosis of young mesenchymal stem cells through staurosporine. The apoptotic body can reduce the expression level of senescence-related factors and promote expression of matrix synthesis-related factors, so that a degeneration microenvironment is improved, extracellular matrix synthesis is promoted, and cell functions are improved. The apoptotic body can be applied to the field of delaying and reversing intervertebral disc degeneration, promotes repair and regeneration of intervertebral disc tissues by delivering rejuvenation signals, provides a new cell-free treatment strategy for treatment of intervertebral disc degeneration, avoids the risks of low cell survival rate, immunological rejection, tumorigenicity and the like, and has a good application prospect. Meanwhile, the traditional limitation of recovering tissue functions through physical or medicine means is broken through.

This invention discloses a mitochondrial micro / nanoreactor system, its preparation method, and its applications. The system comprises mitochondrial-trained apoptotic bodies and a piezoelectric short-fiber scaffold, which are covalently linked via a copper-free click chemical linker. The mitochondrial-trained apoptotic bodies are obtained by inducing apoptosis in macrophages overexpressing Miro1 protein and pretreated with hypoxia, and are rich in functional mitochondria. The piezoelectric short-fiber scaffold is obtained by electrospinning a composite of poly-L-lactic acid and collagen, and can convert external mechanical energy into electrical signals. This invention, through synergistic action, on the one hand, utilizes apoptotic bodies to target and deliver functional mitochondria, enhancing intercellular mitochondrial transfer; on the other hand, it utilizes the piezoelectric effect to generate endogenous electrical stimulation, activating the mitochondrial autophagy pathway, thereby bidirectionally regulating mitochondrial energy metabolism homeostasis in diabetic wounds and significantly promoting wound healing.

Embodiments of the disclosure include treatments of subconcussive and / or concussive brain damage by administering fibroblasts and / or fibroblasts cultured with one or more types of immunocytes. In one specific embodiment fibroblasts are cultured with monocytes in the presence of patient-specific T cells, and subsequently the T cells are re-administered into the patient. In one particular embodiment, products derived from fibroblast-immunocyte mixtures are comprised of cellular lysate, apoptotic bodies, exosomes, and / or other microvesicles. In one embodiment, the fibroblast cells and / or products derived from the fibroblast cells are administered subsequent to one or multiple head injuries. In other embodiments, products are administered in combination with neurorestorative and / or neuroprotective interventions.

The application relates to the fields of biological medicine and tissue engineering materials, and particularly discloses a Janus microneedle capable of promoting inflammation subsiding and blood vessel regeneration of a diabetic wound surface as well as a preparation method and application of the Janus microneedle, the microneedle is a double-layer structure, the upper layer is loaded with an SLC7A11 inhibitor and an apoptotic body, and the lower layer is loaded with an engineered exosome overexpressing miR-17-5p. Through a unique double-layer hydrogel matrix design and a two-phase crosslinking process, the Janus microneedle realizes rapid release of active ingredients in the upper layer in the early healing stage to promote inflammation subsiding and phagocytosis, and slow release of active ingredients in the lower layer in the middle and late healing stage to continuously promote angiogenesis. The microneedle patch can intelligently simulate and synergistically regulate the natural timing of wound healing, solves the problem of space-time disconnection of the existing single therapy, and provides a novel and precise delivery strategy for efficient repair of chronic and difficult-to-heal wounds such as diabetes.

The invention relates to a double-layer detachable microneedle which comprises a soluble microneedle tip and a detachable hydrogel substrate layer, and the microneedle tip layer comprises at least one of drugs, extracellular vesicles, macromolecular substances and combinations of the drugs, the extracellular vesicles and the macromolecular substances. The extracellular vesicles are one or a combination of a plurality of exosomes, microvesicles, apoptotic bodies, autophagy-related extracellular vesicles, matrix vesicles and stress-related extracellular vesicles, and the macromolecular substances are one or a combination of a plurality of polypeptides, proteins, antigens, enzymes and antibodies. According to the double-layer detachable microneedle, early-stage antibiosis and pH adjustment can be achieved, infection is reduced, and inflammation is inhibited; macrophages are induced to be polarized from M1 to M2, and inflammation is promoted to be transited to a proliferation stage; needle tip loading is continuously released, and angiogenesis, cell proliferation and tissue regeneration are enhanced; the double-layer structure can sequentially respond to different stage requirements of the wound, and the healing efficiency of the diabetic wound is remarkably improved.

The present application relates to a kind of engineered stem cell nanometer apoptotic bodies, its preparation method includes the following steps: by 3,3'-diindolylmethane directional induction obtains the engineered stem cell rich in repair and anti-inflammatory signal molecules, and then the engineered stem cell is induced to apoptosis, and the supernatant is separated and obtained by collection, then the programmed tandem extrusion process is handled to the engineered apoptotic body, and the particle size uniformity of the engineered nanometer apoptotic body is prepared.The engineered nanometer apoptotic body of the present application can effectively improve the microenvironment around hair follicle and regulate hair growth cycle, significantly improve the hair density and regeneration rate of androgenetic alopecia model mice, provide a new strategy of safe, convenient and efficient for clinical hair regeneration treatment.

The invention provides an adipose-derived stem cell apoptotic body as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: step (1), obtaining adipose-derived stem cells by a type I collagenase digestion method, and culturing in vitro to an induction period; and (2) taking the adipose-derived stem cells in the induction period, inducing concentrated apoptosis by an STS method, adding trypsin for digestion, and carrying out differential centrifugation at room temperature to obtain a precipitate, namely the adipose-derived stem cell apoptotic body. It is found for the first time that the adipose-derived stem cell apoptotic body has the effect of inhibiting CDC20 expression in hypertrophic scar fibroblasts so as to reduce Wnt / beta-catenin signal channels, and meanwhile, the adipose-derived stem cell apoptotic body has the remarkable inhibiting effect on pathological phenotypes and transverse migration of the hypertrophic scar-derived fibroblasts. In addition, the method is simple to operate, low in cost, safe, efficient and convenient for clinical popularization and application.