Cell microcapsule and preparation method thereof, cell microcapsule loaded with cancer resisting medicines, and preparation method and application of cell microcapsule loaded with cancer resisting medicines
An anticancer drug and cell technology, which is applied in the field of biology and medicine, can solve the problems of lack of biological activity and binding, and achieve the effect of precise treatment targeting, low cost and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0060] One embodiment of the present invention provides a method for preparing cell microcapsules, comprising the following steps:
[0061] (1) Cultivate immune cells and take cell supernatant;
[0062] (2) centrifuging the cell supernatant described in step (1) to remove cells and cell debris, and taking the supernatant;
[0063] (3) centrifuging the supernatant described in step (2) to remove apoptotic bodies, and taking the supernatant;
[0064] (4) centrifuging the supernatant described in step (3), and taking the precipitate to obtain final product;
[0065] The centrifugation conditions described in step (3) include: the centrifugal force is 2000-3000g, and the centrifugation time is 40-100min.
[0066] The preparation method is very simple to operate and can obtain cell microcapsules with good biological activity, complete structure and high purity.
[0067] In the preparation method, the centrifugation condition is further preferably a differential centrifugation me...
Embodiment 1
[0085] Example 1 Cell microcapsules derived from macrophages loaded with doxorubicin
[0086] 1. Preparation of fat-soluble doxorubicin
[0087] 1. Weigh 20mg of doxorubicin hydrochloride into a 100ml round bottom flask, add 20ml of dichloromethane to fully dissolve.
[0088] 2. Add 14.3ml of triethylamine according to the molar ratio of 1:3, and stir overnight at 25°C.
[0089] 3. After 24 hours, collect the product and place it in a dialysis bag for dialysis until the dialysate is clear and colorless.
[0090] 4. Collect the material in the dialysis bag and freeze-dry it to obtain dark red doxorubicin powder.
[0091] 5. Dissolve 543.52 μg of doxorubicin powder in 1 mL of DMSO to prepare a doxorubicin solution with a final concentration of 1 mM.
[0092] 2. Preparation of cell microcapsules
[0093] In 95% air and 5% CO 2 In a cell culture incubator, in a sterile and non-toxic environment, at a culture temperature of 35°C-37°C, continuously culture macrophages (RAW264.7...
Embodiment 2
[0100] Example 2 Cell microcapsules derived from macrophages loaded with paclitaxel
[0101] 1. Configuration of paclitaxel stock solution
[0102] Weigh 853.91 μg of paclitaxel powder and dissolve it in 1 mL of DMSO to prepare a paclitaxel solution with a final concentration of 1 mM.
[0103] 2. Preparation of cell microcapsules
[0104] In 95% air and 5% CO 2 In a cell culture incubator, in a sterile and non-toxic environment, at a culture temperature of 35°C-37°C, continuously culture macrophages (RAW264.7) in a complete medium containing 10% serum for more than 2 days, collect the cell supernatant for micro Capsule extraction and purification, the experimental steps are as follows:
[0105]1. Remove cells and cell debris: Centrifuge the above cell supernatant at 500g for 10 minutes, discard the precipitate, and take the supernatant;
[0106] 2. Remove apoptotic bodies: centrifuge the supernatant at 2500 g for 1 hour in step (1), discard the precipitate, and take the su...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com