Rapid and high-purity separation method of human plasma exosome
A separation method, human plasma technology, applied in the direction of material separation, analysis materials, measuring devices, etc., can solve the problems of low purity, low yield, and reduce the purity of exosomes
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[0022] The agarose gel column series high performance liquid phase exclusion chromatography of the present invention is used for rapid and high-purity separation of human plasma exosomes, and the specific steps are as follows:
[0023] (1) Plasma acquisition: centrifuge the human blood collected with EDTA collection tube at 500g for 8min, and take the upper layer liquid, which is the human plasma sample, the volume is 2mL;
[0024] (2) Plasma pretreatment: centrifuge the human plasma collected in (1) at 12000g for 15min, discard the precipitate, take the upper liquid, and then filter it with a 0.22um filter membrane;
[0025] (3) Filling of agarose gel column: absorb 12mL of purchased 4B or CL-4B agarose ethanol solution, replace it with PBS solution 3 times, then pour it into a gravity flow empty tube (total volume of 12mL) , after the agarose settles freely, open the lower end of the flow tube to let the solution flow out, then push the upper sieve plate into the flow tube w...
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