Preparation of broad spectrum antitumor mouse mold with apoptotic body vaccine immunity

A technology of apoptotic body and mouse model, which is applied in the field of anti-tumor animal experiment research, can solve problems such as unreasonable design and unstable anti-tumor effect

Active Publication Date: 2018-12-18
庆云堂生物科技(北京)有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation of broad-spectrum anti-tumor mice under the immune mechanism and its model establishment in view of the unstable an

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  • Preparation of broad spectrum antitumor mouse mold with apoptotic body vaccine immunity
  • Preparation of broad spectrum antitumor mouse mold with apoptotic body vaccine immunity
  • Preparation of broad spectrum antitumor mouse mold with apoptotic body vaccine immunity

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Embodiment Construction

[0019] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0020] The method of obtaining broad-spectrum anti-tumor mice: the first batch (2-3) Kunming mice were intraperitoneally inoculated with 1×10 7 Left or right H22 mouse liver cancer cells to make them grow into ascites tumors. After about 10 days, after the mouse ascites tumors grow to the logarithmic phase, extract an appropriate amount of ascites from the abdominal cavity and use it at a ratio of 1:5 under sterile conditions. The erythrocyte lysate was used to remove the erythrocytes, washed twice with normal saline after centrifugation, and centrifuged to obtain H22 mouse liver cancer cells. Place H22 cells in medium containing 10% fetal bovine serum at 37°C with 5% CO 2 The H22 cells were culture...

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Abstract

The invention provides preparation of broad spectrum antitumor mouse mold with apoptotic body vaccine immunity, wherein the mouse is prepared through the apoptotic body vaccine immunity and has the broad spectrum antitumor property. An establishment method includes: 1) in vitro, inducing apoptosis of cancer cells through a nutrient deficiency process to acquire the apoptotic body; 2) immunizing aKunming mouse with the apoptotic body by subcutaneous injection of 25 mg/kg of the apoptotic body to every mouse once per week, the injection lasting for five weeks in total. In vivo antitumor experimental results of the mouse prove that the anti-tumor rate on H22 tumor-bearing mice can reach 80.27% and model forming successful rate can reach 50%. The invention directs at problems that in a present experiment of immunizing antitumor animals, a positive control model is not reasonable and there is no broad spectrum immuno-antitumor mouse on market, whereas the mouse immunized by the apoptotic body solves the problem. The product has stronger antitumor immunity, is stable in model and is high in production quality.

Description

technical field [0001] The invention belongs to the research field of anti-tumor animal experiments, and relates to the research on the establishment of anti-tumor animal models, in particular to an immune mouse with broad-spectrum anti-tumor effect, its preparation method and its application in anti-tumor animal experiments. Background technique [0002] As an indispensable part of the development of modern science and technology, animal experiments are to explore the mysteries of human life, cure diseases and prolong life by extending from animal life phenomena to human life activities. Since the incidence and mortality of cancer in China have been rising, many scholars have been working hard to study anti-tumor drugs. [0003] At this stage, in the design of animal experiments of most anti-tumor drugs, the experimental animals will be divided into normal group, model group, positive control group and experimental drug group. In the positive control group, antitumor drugs...

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Application Information

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IPC IPC(8): A61K39/00A61K35/13A61P35/00A01K67/027
CPCA61K39/00A61P35/00A01K67/0271A61K35/13A01K2267/0331A01K2227/105A01K2207/12
Inventor 刘安军王喜玲
Owner 庆云堂生物科技(北京)有限公司
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