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Apoptotic body preserving solution and method for preserving apoptotic body

A technology of apoptotic body and cryopreservation solution, which is applied in the field of long-term preservation of mesenchymal stem cell apoptotic body in vitro. Issues such as the long-term storage method of apoptotic bodies are not disclosed, so as to achieve the effects of convenient industrial application, high safety, and reduced removal of impurities

Inactive Publication Date: 2019-10-25
山东佰鸿干细胞生物技术有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] After searching, it is found that the current patent application number CN201580039370.9 on the application of apoptotic bodies describes the medical treatment application and isolation method of apoptotic bodies, but does not elaborate on the preservation and application of apoptotic bodies. The prior art does not disclose the long-term preservation method of apoptotic bodies, which is not convenient for large-scale industrial application

Method used

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  • Apoptotic body preserving solution and method for preserving apoptotic body
  • Apoptotic body preserving solution and method for preserving apoptotic body
  • Apoptotic body preserving solution and method for preserving apoptotic body

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Embodiment 1

[0056] This embodiment provides a cryopreservation solution for apoptotic bodies. According to parts by weight, the cryopreservation solution comprises the following components: 5 parts of hydroxyethyl starch, 5 parts of glycerin, 20 parts of human serum albumin, 5 parts of trehalose 1 part of vc injection, 10 parts of amino acid injection, 5 parts of glucose injection and 49 parts of normal saline.

Embodiment 2

[0058] This embodiment provides a cryopreservation solution for apoptotic bodies. According to parts by weight, the cryopreservation solution comprises the following components: 6 parts of hydroxyethyl starch, 5 parts of glycerin, 10 parts of human serum albumin, and 5 parts of trehalose. 0.1 part of vitamin C injection, 5 parts of amino acid injection, 1 part of glucose injection and 45 parts of sodium lactate Ringer injection.

Embodiment 3

[0060] This embodiment provides a cryopreservation solution for apoptotic bodies. According to parts by weight, the cryopreservation solution comprises the following components: 7 parts of hydroxyethyl starch, 5 parts of glycerin, 12 parts of human serum albumin, and 5 parts of trehalose. 0.2 parts of vitamin C injection, 6 parts of amino acid injection, 2 parts of glucose injection and 46 parts of sodium lactate Ringer injection.

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Abstract

The invention provides an apoptotic body preserving solution and a method of long-term in-vitro preservation of an apoptotic body. The apoptotic body preserving solution is prepared from the followingcomponents: a cryoprotectant, an antioxidant and a buffer. According to the technical scheme, a stable apoptotic body source can be provided for stem cell therapy and beauty industry transformation.

Description

technical field [0001] The invention belongs to the technical field of apoptotic bodies, and in particular relates to a preservation solution for long-term preservation of apoptotic bodies of mesenchymal stem cells in vitro and a method for preserving apoptotic bodies. Background technique [0002] Mesenchymal stem cells are a research branch of stem cells. Stem cells are a type of cells with self-replication and multi-directional differentiation capabilities. They can continuously renew themselves and transform into one or more types of human tissues or organs under specific conditions Cell. Stem cells are the origin cells of the body and the progenitor cells that form various tissues and organs of the human body. [0003] Clinical research on mesenchymal stem cells has been carried out in many countries. The United States has approved more than 60 clinical trials. With the maturation of mesenchymal stem cells and related technologies, my country has also approved a number...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 程蕊苹
Owner 山东佰鸿干细胞生物技术有限公司
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