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Tumor cell apoptotic bodies induced by CO2 shock method and anti-tumor application of tumor cell apoptotic bodies

A tumor cell apoptosis, tumor cell technology, applied in the CO2 shock method to induce tumor cell apoptotic body, anti-tumor application, tumor cell apoptotic body field, can solve inaccurate results, low immune activity, whole cell Tumor vaccines cannot effectively solve problems such as

Inactive Publication Date: 2019-02-01
庆云堂生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods need to control many variables such as temperature, CO2 concentration, time, etc., which will cause inaccurate results
At the same time, whole-cell tumor vaccines prepared by physical methods cannot effectively solve the problem of weak antigenicity of tumor cells, so their immune activity is relatively low and their anti-tumor efficiency is low

Method used

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  • Tumor cell apoptotic bodies induced by CO2 shock method and anti-tumor application of tumor cell apoptotic bodies
  • Tumor cell apoptotic bodies induced by CO2 shock method and anti-tumor application of tumor cell apoptotic bodies
  • Tumor cell apoptotic bodies induced by CO2 shock method and anti-tumor application of tumor cell apoptotic bodies

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Embodiment

[0026] Incubate the liver cancer cell HepG2 cells to the logarithmic growth phase, put into the 2 In the incubator to induce apoptosis of tumor cells; after cell apoptosis, collect the supernatant of the cell culture medium, centrifuge at 800g for 10min at room temperature, and after 10min, in order to remove the residual mixed cells and cell debris in the supernatant, take again Repeat the centrifugation of the supernatant once; then slowly move the supernatant to a 10ml sterile syringe, install a filter membrane filter device, apply slight pressure, and slowly let the supernatant pass through the 5μm filter membrane; collect the filtrate in a centrifuge tube, put it into a high-speed Centrifuge at 16,000 g for 20 min at 4°C in a centrifuge; the resulting gray precipitate is the tumor cell apoptotic body.

[0027] The apoptotic bodies of tumor cells were purified by differential centrifugation, and then preserved by adding a preservative until use.

[0028] 1. In vitro index...

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Abstract

The invention relates to tumor cell apoptotic bodies induced by a CO2 shock method and anti-tumor application of the tumor cell apoptotic bodies, wherein the tumor cell apoptotic bodies are obtained by placing tumor cells into a CO2-free incubator to cause apoptosis of the tumor cells. The tumor cell apoptotic bodies are collected by controlling single variables and utilizing separation and purification techniques, and in-vivo anti-tumor testing and in-vitro index detection can be simultaneously performed. The results show that the state and quantity of the collected apoptotic bodies are precisely grasped, and the collected apoptotic bodies have a strong anti-tumor effect.

Description

technical field [0001] The invention belongs to the field of anti-tumor technology, and relates to tumor cell apoptotic bodies, in particular to a CO 2 Shock method induces apoptotic bodies of tumor cells and its anti-tumor application. Background technique [0002] At present, the apoptotic bodies of tumor cells are mainly obtained by inducing apoptosis through ultraviolet irradiation, heat stress and other methods. However, these methods need to control many variables such as temperature, CO2 concentration, time, etc., which will cause inaccurate results. At the same time, whole-cell tumor vaccines prepared by physical methods cannot effectively solve the problem of weak antigenicity of tumor cells, so their immune activity is relatively low and their anti-tumor efficiency is low. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a CO 2 The shock method induces the apoptotic bodies of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A61K39/00A61P35/00
CPCA61K39/0011A61P35/00C12N5/0693
Inventor 刘安军呂淑明
Owner 庆云堂生物科技(北京)有限公司
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