Tumor-targeted delivery carrier based on cell-derived micro-vacuoles, preparation method and application

A technology for tumor targeting and delivery vectors, which is applied in the field of preparation of tumor targeting delivery vectors and can solve the problem of low yield
CN106692984AActive Publication Date: 2017-05-24珈泌生物科技(武汉)有限责任公司
5 Cites 14 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
珈泌生物科技(武汉)有限责任公司
Publication Date
2017-05-24

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a tumor-targeted delivery carrier based on cell-derived micro-vacuoles, a preparation method and an application. The preparation method comprises the following steps of: (A) preparing a conditioned medium: supplementing fetal bovine serum, antibiotics, DSPE-PEG-Biotin and DSPE-PEG-Folate into a basal medium; (B) using the obtained conditioned medium in cell culture, and collecting cell culture supernatant for subsequent separation; (C) carrying out low-speed centrifugation on the obtained culture supernatant to remove cell debris and apoptotic bodies, then adding SA-IONPs, mixing uniformly, incubating, then separating by a magnet, using PBS for re-suspension, eluting for multiple times to obtain the cell-derived micro-vacuoles with membrane surfaces modified by folic acid and iron oxide nano-particles, and freezing for storage; and (D) loading chemotherapeutic drugs or therapeutic genes into the functionalized micro-vacuoles doubly-modified by an electroporation mode, and carrying out re-suspension after separation with the magnet. The tumor-targeted delivery carrier based on cell-derived micro-vacuoles, the preparation method and the application disclosed by the invention are applicable to specific targeting delivery of multiple chemotherapeutic drugs and therapeutic genes, and have the advantages of enhancing the anti-tumor effect, reducing the systemic toxicity and improving the clinical effect of the current therapeutic selection, so that a new hope is brought for clinical therapy of tumors.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to the technical field of new dosage forms and preparations of biology and medicine, more specifically to a tumor-targeted delivery carrier based on cell-derived microvesicles, and also to a tumor-targeted delivery carrier based on cell-derived microvesicles The preparation method is convenient and efficient, and simultaneously realizes the folic acid and magnetic dual labeling on the surface of cell-derived microvesicles; it also involves a targeted delivery carrier based on cell-derived microvesicles in the preparation of local chemotherapy drugs for treating or preventing tumors and The application of gene therapy vectors is suitable for the use of this tumor-targeted delivery vector in scientific research and clinical practice for the specific targeted delivery of drugs or therapeutic genes to prevent or treat the following diseases: 1. Cervical cancer, ovarian cancer , endometrial cancer, testicular cancer, gastric canc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More