Preparation method and application of anti-tumor vaccine based on cell microvesicles

A microvesicle, anti-tumor technology, applied in the field of medicine, achieves the effect of simple method, wide source and powerful killing effect

Inactive Publication Date: 2020-03-24
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report in the prior art that tumor cell microvesicles are used as nano iron ferric oxide carriers to capture adjuvant liposomes as vaccines to achieve anti-tumor therapy

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  • Preparation method and application of anti-tumor vaccine based on cell microvesicles
  • Preparation method and application of anti-tumor vaccine based on cell microvesicles
  • Preparation method and application of anti-tumor vaccine based on cell microvesicles

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Embodiment Construction

[0034] The specific implementation of the present invention will be described in detail below in combination with specific situations.

[0035] In the specific implementation of the present invention, a method for preparing an anti-tumor vaccine based on cell microvesicles comprises the following steps:

[0036] 1) Preparation of mouse-derived B16F10 melanoma cell microvesicles loaded with immunomodulator nano-ferric oxide:

[0037] (1) Place mouse B16F10 melanoma cells in RIPM1640 medium containing 10% fetal bovine serum at 37°C, saturated humidity, 5% CO 2 Cultivated in an incubator;

[0038] (2) Mix the immunomodulator nano-iron ferric oxide with RIPM1640 medium containing fetal bovine serum and add it to the adherent-growing murine B16F10 melanoma cells, and irradiate the murine B16F10 melanoma cells with ultraviolet rays for 60 minutes to make them Apoptosis occurs;

[0039] (3), continue to place at 37 ℃, saturated humidity, 5% CO 2Incubate the mouse-derived B16F10 m...

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Abstract

The invention relates to a preparation method and application of an anti-tumor vaccine based on cell microvesicles and aims to effectively solve problems in preparation of anti-tumor vaccines high inyield, good in universality, strong in killing effect on tumor cells and capable of controlling tumors by improving tumor microenvironments, repairing an immune system and enhancing anti-tumor immuneresponse of organisms. The preparation method comprises the following steps: preparing tumor cell microvesicles loaded with immunomodulators and connecting the surfaces of the microvesicles with adjuvant-loaded liposome to stably form the anti-tumor vaccine. The prepared anti-tumor vaccine is high in yield, good in universality, strong in killing effect on tumor cells and capable of improving tumor microenvironments, repairing an immune system and enhancing anti-tumor immune response of organisms; the method is simple in process and high in efficiency; and the prepared microvesicles are derived from cells, are sufficient in quantity and wide in source, are easy for large-scale production, improve the bioavailability of adjuvants, are effectively applicable to preparation of anti-tumor vaccines based on the cell microvesicles, and can be applied to prevention and treatment of different types of tumors.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of an anti-tumor vaccine based on cell microvesicles. Background technique [0002] From the immune checkpoint inhibitor CTLA-4 antibody in 2011, to the approval of PD-1 in 2014, and to the approval of CAR-T cell immunotherapy in 2017, tumor immunotherapy has frequently received good news. This major scientific breakthrough is expected to become a cancer treatment mainstream. As an important branch of tumor immunotherapy, tumor vaccines have also become a hot spot in the field of tumor treatment research in recent years. Tumor vaccines express specific and immunogenic tumor antigens (such as: tumor cell lysates, tumor-associated proteins or peptides, etc.), with the assistance of cytokines, chemokines and other adjuvants, to restart and maintain tumors -Immune cycle, restore the body's normal anti-tumor immune response, so as to control and eliminate tumor cells....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/39A61K45/06A61P35/00
CPCA61K39/0011A61K39/39A61K45/06A61P35/00A61K2039/55505A61K2039/55555A61K2039/55516A61K2039/55561A61K2039/876A61K2300/00
Inventor 赵洪娟王蕾张振中赵倍倍吴丽遐
Owner ZHENGZHOU UNIV
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