48results about How to "Improve immunosuppression" patented technology

An objective of the present invention is to provide novel therapeutic agents for cancer, which have an excellent antitumor effect in cancer patients by enhancing their immune function. The present inventors discovered that the administration of at IL-6 inhibitor and / or gemcitabine or a salt thereof to tumor-bearing organisms yields an excellent antitumor T cell response-enhancing effect, and completed the present invention. In addition, the present inventors discovered that the T cell response-enhancing effect produces an excellent antitumor effect.

A medicine which effectively functions as an immunosuppressant or anti-inflammatory agent and is effective in diminishing the occurrence of side effects. The medicine comprises a combination of: a diaryl sulfide or diaryl ether compound having a 2-amino-1,3-propanediol structure and having the function of diminishing lymphocytes circulating through the periphery; and an immunosuppressant and / or an anti-inflammatory agent.

The invention discloses an umbilical cord mesenchymal stem cell exosome, a preparation method and an application of the exosome to a cosmetic. The exosome released by mesenchymal stem cells has the characteristics of the stem cells and has various biological functions of promoting tissue repair, immune suppression, nerve protection and the like. Although the mesenchymal stem cells are regarded asthe strongest cells generating the exosome, market requirements are still difficultly met when the exosome is used as a cosmetic raw material. The umbilical cord mesenchymal stem cell exosome has thefunction of promoting human skin fibroblast proliferation, the human skin fibroblast proliferation promoting function of the exosome can be remarkably improved after the mesenchymal stem cells are modified and transformed to highly express miR-525-3p, the skin aging resistance activity of unit mass of the exosome is remarkably improved, and the umbilical cord mesenchymal stem cell exosome and themiR-525-3p high-expression umbilical cord mesenchymal stem cell exosome can be used for the cosmetic resisting skin aging.

The invention provides a pre-processing method for enhancing immune regulation capacity of mesenchymal stem cells. The mesenchymal stem cells are pre-processed in vitro by virtue of various protein factor combinations, so that an optimum factor proportion is found, and subsequently, the immune regulation capacity of the mesenchymal stem cells is rapidly enhanced. With the application of the short-time pre-processing method disclosed by the invention, the immune regulation capacity of the mesenchymal stem cells to peripheral blood lymphocytes is significantly enhanced; therefore, the culture method is conducive to the improvement of a clinical treatment effect on autoimmune diseases and immune disorder related diseases.

Disclosed herein are genetically modified cells expressing HLA-G (e.g., cell surface HLA-G) persistently, and nucleic acid compositions useful for generating such genetically modified cells. Also disclosed are cell therapy methods that utilize genetically modified cells that express HLA-G persistently. The HLA-G genetic modifications described herein provide the cells with characteristics of reduced immunogenicity and / or improved immunosuppression, such that these cells have the promise of being universal or improved donor cells for transplants, cellular and tissue regeneration or reconstruction, and other therapies.

An objective of the present invention is to provide novel therapeutic agents for cancer, which have an excellent antitumor effect in cancer patients by enhancing their immune function. The present inventors discovered that the administration of at IL-6 inhibitor and / or gemcitabine or a salt thereof to tumor-bearing organisms yields an excellent antitumor T cell response-enhancing effect, and completed the present invention. In addition, the present inventors discovered that the T cell response-enhancing effect produces an excellent antitumor effect.

The invention relates to mesenchymal stem cell membrane-coated bionic nanoparticles with overexpressed PD-L1 molecules on the surface as well as preparation and application of the mesenchymal stem cell membrane-coated bionic nanoparticles. The mesenchymal stem cell membrane-coated bionic nanoparticle with the overexpressed PD-L1 molecules on the surface comprises a nano-core and a mesenchymal stemcell membrane wrapping the nano-core, the nano core comprises a polymer with biocompatibility, and PD-L1 molecules are overexpressed on the membrane surface of the mesenchymal stem cell membrane. Theinvention also discloses an application of the mesenchymal stem cell membrane-coated bionic nano-particle with over-expressed PD-L1 molecules on the surface in preparation of an inflammation treatment preparation. The bionic nanoparticle has a remarkable immunosuppression effect, is high in biocompatibility and simple and mature in preparation process, can be used for preparing an inflammation treatment preparation, can be effectively enriched in an inflammation part, and shows an excellent curative effect in inflammation treatment by inhibiting excessively activated immune response and cytokine storm of the inflammation part.

The invention relates to a preparation method and application of an anti-tumor vaccine based on cell microvesicles and aims to effectively solve problems in preparation of anti-tumor vaccines high inyield, good in universality, strong in killing effect on tumor cells and capable of controlling tumors by improving tumor microenvironments, repairing an immune system and enhancing anti-tumor immuneresponse of organisms. The preparation method comprises the following steps: preparing tumor cell microvesicles loaded with immunomodulators and connecting the surfaces of the microvesicles with adjuvant-loaded liposome to stably form the anti-tumor vaccine. The prepared anti-tumor vaccine is high in yield, good in universality, strong in killing effect on tumor cells and capable of improving tumor microenvironments, repairing an immune system and enhancing anti-tumor immune response of organisms; the method is simple in process and high in efficiency; and the prepared microvesicles are derived from cells, are sufficient in quantity and wide in source, are easy for large-scale production, improve the bioavailability of adjuvants, are effectively applicable to preparation of anti-tumor vaccines based on the cell microvesicles, and can be applied to prevention and treatment of different types of tumors.

Provided are methods and compositions for obtaining functionally enhanced derivative effector cells obtained from directed differentiation of genomically engineered iPSCs. The derivative cells provided herein have stable and functional genome editing that delivers improved or enhanced therapeutic effects. Also provided are therapeutic compositions and the used thereof comprising the functionally enhanced derivative effector cells alone, or with antibodies or checkpoint inhibitors in combination therapies.

An extract of prunella spike for treating rheumatoid arthritis and improving immune suppression contains phenolic acid (60-70%) and rosemaric acid (14-21%). Its preparing process is also disclosed.

The invention discloses an application of a traditional Chinese medicinal composition in the preparation of anti-ageing medicines. The traditional Chinese medicinal composition is a pure traditional Chinese medicinal preparation prepared through using Cuscuta chinensis Lam., wolfberry fruit, Chinese magnoliavine and the like. Tests prove that the traditional Chinese medicinal composition can improve the learning and memory capability decline and immune suppression caused by ageing, and has a certain anti-oxidation effect.

The invention provides a health care product or medicine composition capable of relieving fatigue and enhancing immunity; the health care product or medicine composition is in a dosage form prepared from the following medicinal raw materials in parts by weight: 1-45 parts of cordyceps militaris, 2.5-75 parts of rhizoma polygonati, 2-60 parts of fruit of Chinese wolfberry, 2-60 parts of raspberry, 2-75 parts of mulberry, 3-75 parts of arillus longan, 5-150 parts of yam, 2-75 parts of jujube and 2-60 parts of hawthorn. The invention also provides a preparation method and application of the health care product or medicine composition. Various groups of the composition disclosed by the invention, differing in proportion, are excellent in immunity enhancing effect, and are capable of significantly increasing the number of blood cells, improving the immunosuppression of chemotherapy drugs on a body and effectively relieving fatigue; and the composition offers a new choice for clinical medication.

The invention provides a composition for preventing pneumoconiosis and a preparation method thereof, wherein the composition is prepared from cordyceps militaris, frosted mulberry leaves, tea polyphenols, gingkgo, fructus monordicae extracts, barbary wolfberry fruits, peach kernels, apricot kernels, and malto dextrin, and the preparation method comprises the steps of: (1) soaking the gingkgo, barbary wolfberry fruits, frosted mulberry leaves, peach kernels and apricot kernels in water; (2) heating the soaked materials to be boiling, leaching, then carrying out flocculation precipitation on leach liquor, and concentrating a supernatant fluid into a thick paste; (3) continuously adding water into the rest materials, heating and leaching; (4)mixing and concentrating the two-time extracts into a thick paste, carrying out vacuum drying and then crushing; (5) sieving the extract, and fully mixing with the cordyceps militaris nano powder, malto dextrin, tea polyphenols and fructus monordicae extracts; and (6) fully mixing the extract with the mixture to be uniform, mixing with alcohol, and palletizing. The composition has an obvious free radical scavenging action, reduces ceruloplasmin, obviously restrains pneumoconiosis fibrosis, and greatly improves the respiratory symptoms of patients with pneumoconiosis.

The invention provides cell culture fluid of serum-free cultivation for enhancing immunosuppression function of animal mesenchymal stem cells. Multiplication factors and specific additives are added into a basic nutrient solution for serum-free cultivation of animal mesenchymal stem cells, in order to promote healthy growth and proliferation of animal mesenchymal stem cells, and provide functions for maintaining and enhancing immunosuppression of animal mesenchymal stem cells. The fluid has the function for enhancing function of animal mesenchymal stem cells. Experiments prove that the serum-free culture fluid for enhancing immunosuppression function of animal mesenchymal stem cells can be used for serum-free cultivation of animal mesenchymal stem cells, and can be applied to clinic research and application in the aspect of immunosuppression application research of mesenchymal stem cells.

The invention belongs to the field of medicines and discloses a triptolide and chinese brake herb general flavone combined medicinal composition and preparation and application thereof. The medicinal composition is prepared by uniformly mixing 1 weight part of triptolide and 10<-3> to 10<5> weight part of chinese brake herb general flavone. The chinese brake herb general flavone is prepared from chinese brake herb through ethanol solution extraction, organic solvent distribution, and macroporous resin and polyamide column refining. The medicinal composition can be applied to preparation of medicines for treating rheumatoid arthritis, anti-tumor medicines, immunosuppression medicines, and anti-inflammatory medicines.

The invention provides a stem cell preparation for treating aplastic anemia and a preparation method thereof. The defects in the prior art are overcome, the occurrence rate of the graft versus host disease is decreased, more mesenchyme stem cells with the high immunosuppression function are obtained within a short period of time, the clinical requirements are met, a new thought is provided for the transplantation effect of stem cells, and important practical application value is brought into play. The invention further provides the preparation method of the stem cell preparation for treating aplastic anemia. According to the method, the mesenchymal stem cells are induced and cultivated through a special method, the immunosuppression capacity of the mesenchymal stem cells can be effectively improved and enhanced, and the mesenchymal stem cells can be further prepared into the stem cell preparation for treating aplastic anemia.

The invention relates to a sustained-release tacrolimus eye-drops preparation. The sustained-release tacrolimus eye-drops preparation is characterized by being prepared from tacrolimus playing a pharmacological role, radix astragali and / or pseudo-ginseng serving as a mucosal adhesive polymer medicine release system, and pharmaceutically acceptable pharmaceutical adjuvants for local eyes, wherein by weight, 100 parts of sustained-release tacrolimus eye-drops preparation contains 0.01-1.0 part of tacrolimus, the weight ratio of the tacrolimus to the radix astragali and / or pseudo-ginseng is 1 to (0.5-5.0), and the weight ratio of the radix astragali to the pseudo-ginseng is 1 to 3. The sustained-release tacrolimus eye-drops preparation prepared by a preparation method has the advantages of good intraocular penetration, high intraocular bioavailability, little toxic or side effect and capabilities of treating and preventing ophthalmic immune-related diseases such as autoimmune uveal retinitis.

The invention provides a macrophage exclusive chimeric antigen receptor, a controllable polarized mononuclear / macrophage for expressing the macrophage exclusive chimeric antigen receptor, and a preparation method and application thereof, and relates to the field of biotechnology. The invention provides a chimeric antigen receptor which comprises an extracellular antigen binding domain, a transmembrane domain and an intracellular activation domain which are sequentially connected, and the extracellular antigen binding domain comprises signal peptide and / or scFv and can specifically recognize cell membrane surface protein EGFRvIII specifically expressed by GBM; the transmembrane domain comprises CD8alpha which linking the extracellular antigen binding domain and the intracellular activation domain; an intracellular activation domain comprises TIR, CD3ZETA or GM-CSFRalpha / beta and promotes polarization of macrophages to M1, the chimeric antigen receptor is introduced into the macrophages, the macrophages are endowed with a targeted killing effect on GBM, and the M1 polarization state of the macrophages is effectively promoted and maintained.

The present invention relates to a method of suppressing organ rejection in a patient receiving an organ transplant by initiating oral treatment with a once-daily extended release tacrolimus dosage form, for example, at an initial dose of from about 0.15 to about 0.20 mg / kg / day within 24 or 48 hours following transplantation. The once-daily extended release tacrolimus dosage form (i) provides low fluctuation and / or swing of tacrolimus, (ii) provides a significantly lower Cmax than an immediate release formulation of tacrolimus while providing the same or greater area under the curve (AUC), (iii) releases the tacrolimus substantially in the colon and / or the lower ileum, (iv) releases at most 63.5% of the tacrolimus in the dosage form at the 12 hour time point, or (v) any combination of any of the foregoing.

In order to solve the problem of limited stem cell immunosuppressive ability existing in the prior art, the present invention relates to the fields of adult stem cell and cell therapy, and relates toa novel application of mesenchymal stem cells, in particular to a method for enhancing immunosuppression by over-expressing IDO by umbilical cord mesenchymal stem cells. The method is characterized byconstructing a lentivirus overexpressing an IDO gene, constructing the umbilical cord mesenchymal stem cell overexpressing IDO by a lentiviral transfection system, and adding a gene opening agent; and co-culturing the umbilical cord mesenchymal stem cell expressing the IDO and the immune cells, which greatly improves the immunosuppressive ability of the umbilical cord mesenchymal stem cells, reduces the number of clinical use of stem cells, and save a lot of time, labor and material costs.

The invention discloses a triptolide and lonicerin compatible medicine composition, a preparation and an application thereof, belonging to the field of medicines. The medicine composition is prepared from the following bulk pharmaceutical chemicals in parts by weight: 1 part of triptolide and 10<-3>-10<5> parts of lonicerin through the step of uniformly mixing, wherein the lonicerin has a chemical name of 3',4',-5,7-tetrahydroxy flavones-7-O-alpha-L-rhamnose (1->2)-beta-D-glucoside. The medicine composition is applied to the preparation of the medicine for treating rheumatoid arthritis, tumors, immunodepression and inflammation.

The invention relates to application of mesenchymal stem cell-derived small extracellular vesicles overexpressing interleukin 10 in preparation of drugs for treating autoimmune diseases. The MSCs-derived sEVs overexpressing IL-10 not only have the common characteristics and advantages of sEVs, such as easy transportation and storage, repeated freezing and thawing, low immunogenicity and the like,but also combine the strong immunosuppressive capacity of IL-10, so that the MSCs-derived sEVs overexpressing IL-10 has a great potential clinical application value and has the potential of being developed into a new clinical biological preparation, and the innovation and development of ophthalmic drugs are promoted. In addition, sEVs are found to have inflammation chemotactic ability and can be focused to diseased organs to play an immunosuppressive role.

To provide a medicament which efficiently expresses an immunosuppressive agent or an anti-inflammatory agent and reduces expression of side effect.A medicament includes diaryl sulfide or diaryl ether compound having a 2-amino-1,3-propanediol structure having an activity of reducing lymphocytes circulating peripherally, in combination with an immunosuppressive agent and / or an anti-inflammatory agent.

The application provides use of a CSF-1R kinase inhibitor, i.e., a compound with a general formula (A) or pharmaceutically acceptable salts thereof in preparation of drugs for treating CSF-1R kinase signal transduction channel related diseases or drugs for regulating immunity. Shown by in-vivo and in-vitro researches, the compound can be used for remarkably inhibiting the activity of a CSF-1R kinase; the compound can be used for remarkably inhibiting proliferation of a CSF-1 / CSF-1R-driven mouse myeloid leukemia cell line, inhibiting survival of CSF-1-induced macrophages and reversing M2-leant polarization phenotypes of the macrophages, and the effect is superior to that of a drug Pexidartinib on the market; in a TAMs-enriched tumor model (MC38 model), the compound can be used for remarkably antagonizing a tumor immunity inhibiting microenvironment, and a remarkable antitumor drug effect is embodied; and the compound has an inhibiting action on tumors insensitive to immunity check point drugs, can be used for strengthening the treatment effect of the immunity check point drugs and has a very good clinical application prospect.

The present disclosure discloses the Twa1 gene and its expression products and applications. This disclosure first discovers and verifies the role of the Twa1 gene and its expression product in the occurrence and development of cancer. The Twa1 gene and its expression product has the ability to promote cell proliferation, growth, migration, invasion, and tumor formation, thereby providing a diagnosis of malignant tumors. This disclosure also provides new targets and strategies for inhibiting or interfering with the occurrence and development of many malignant tumors. The present disclosure provides an expression vector containing the Twa1 gene and a transgenic cell line and host bacteria containing the expression vector.

The invention discloses an application of a traditional Chinese medicinal composition in the preparation of anti-ageing medicines. The traditional Chinese medicinal composition is a pure traditional Chinese medicinal preparation prepared through using Cuscuta chinensis Lam., wolfberry fruit, Chinese magnoliavine and the like. Tests prove that the traditional Chinese medicinal composition can improve the learning and memory capability decline and immune suppression caused by ageing, and has a certain anti-oxidation effect.

Disclosed herein are genetically modified cells expressing HLA-G (e.g., cell surface HLA-G) persistently, and nucleic acid compositions useful for generating such genetically modified cells. Also disclosed are cell therapy methods that utilize genetically modified cells that express HLA-G persistently. The HLA-G genetic modifications described herein provide the cells with characteristics of reduced immunogenicity and / or improved immunosuppression, such that these cells have the promise of being universal or improved donor cells for transplants, cellular and tissue regeneration or reconstruction, and other therapies.

The invention discloses a non-resistance traditional Chinese medicine formula for enhancing swine fever vaccine immunity and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The problem that an immune effect cannot be effectively achieved after fever vaccine is immunized due to pig immunosuppression is solved. The non-resistance traditional Chinesemedicine formula consists of the following raw materials in parts by weight: 20-30 parts of radix astragali, 15-25 parts of codonopsis pilosula, 18-25 parts of wine herba epimedii, 10-20 parts of polygonum multiflorum, 12-20 parts of fried rhizoma atractylodis macrocephalae, and 3-10 parts of wine-treated glossy privet fruit. The non-resistance traditional Chinese medicine formula is mainly used as a traditional Chinese medicine immunity enhancer.

The invention relates to a traditional Chinese medicine composition for improving the pregnancy rate of sows. The traditional Chinese medicine composition is prepared from, by weight, 15-35 parts of Chinese angelica extract, 25-35 parts of yam extract, 15-30 parts of epimedium extract and 10-25 parts of cinnamon extract. The traditional Chinese medicine composition can improve the pregnancy rate of sows, improve the production performance of the sows and improve the breeding benefits, is small in dosage, good in taste, small in side effect, free of residues and conforms to the modern concept of green, natural and healthy breeding. In addition, the invention further provides a preparation method and application of the traditional Chinese medicine composition.