2results about How to "Improve immunosuppression" patented technology

This invention belongs to the field of biomedical technology, specifically disclosing an injectable embolic agent, its preparation method, and its application. The embolic agent is a hydrocolloid system containing core-shell structured nanoparticles: the core contains Cu... 2+ Salt and ATOX1 inhibitors, where ATOX1 inhibitors can inhibit copper ion efflux and promote intracellular copper accumulation, and interact with Cu 2+ It synergistically induces copper death in tumor cells; the shell is a thermosensitive block copolymer hydrogel that can interact with Cu. 2+ Coordination bonds are formed, enhancing the stability of nanoparticles, while a sol-gel transition occurs at physiological temperatures, enabling precise vascular embolization; the overall core-shell structure allows for the formation of Cu... 2+ The sustained-release effect of ATOX1 inhibitors was observed. In the VX2 rabbit hepatocellular carcinoma model, this embolization agent significantly improved tumor necrosis rate and reduced metastasis rate compared to traditional iodized oil embolization; it also downregulated hypoxia- and angiogenesis-related factors such as HIF-1α, VEGF, and CD31, inhibited MMP9-mediated tumor invasion and metastasis, and promoted CD8+. + T-cell infiltration effectively improves the tumor immunosuppressive microenvironment after transarterial chemoembolization.

The application discloses application of MSCs culture solution in enhancing paracrine and immunosuppression efficiency, wherein a diplotene protein solution is added into a complete MSCs culture medium, i.e., a DMEM / F12 culture medium containing 10% fetal bovine serum, and the MSCs culture solution is obtained after sufficient mixing, wherein the concentration of the diplotene protein in the MSCs culture solution is 1 mu g / ml-500 mu g / ml, and the MSCs culture solution can significantly improve the proliferation capacity, paracrine capacity and immunosuppression efficiency of MSCs without changing the morphology and basic phenotype of the MSCs, the culture solution additive has simple components, can produce significant effects in a short time (24-48h), has wide application prospects, and is favorable for popularization and application.