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Preparation and application of two-aqueous-phase porous pancreas islet microcapsule with core-shell structure

A core-shell structure and microcapsule technology, which is applied in the field of porous pancreatic islet microcapsules and their preparation, can solve the problems of complex preparation of hydrogel microspheres, low transplantation survival rate, decreased cell activity, etc., and achieves low price and repeatability. High, simple method

Pending Publication Date: 2022-01-28
ZHONGDA HOSPITAL SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings of traditional hydrogel-encapsulated cells, such as decreased cell activity, low transplant survival rate, and complicated preparation of hydrogel microspheres, the present invention provides a two-phase aqueous-phase porous islet microcapsule with a core-shell structure and its preparation method

Method used

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  • Preparation and application of two-aqueous-phase porous pancreas islet microcapsule with core-shell structure
  • Preparation and application of two-aqueous-phase porous pancreas islet microcapsule with core-shell structure
  • Preparation and application of two-aqueous-phase porous pancreas islet microcapsule with core-shell structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of porous islet microcapsules with core-shell structure

[0036] (1) Design of microfluidic chip:

[0037] First design the microfluidic EFI chip, select capillary glass tubes with diameters of 800 microns and 300 microns, adjust the outlet of the external phase capillary to 500 microns by pulling the tube, and use the coaxial method to nest the capillary tubes with two diameters. The coaxial capillary is pasted and fixed with a needle at the corresponding place, leaving the inlet of the two-phase solution. Such as figure 1 shown. Repeated washing with ultrapure water to obtain a microfluidic chip.

[0038] (2) Preparation of porous islet microcapsules with core-shell structure:

[0039] In order to make the shell hydrogel have a porous structure, add a small amount of 1% (w / v) PEO solution to the prepared 1% (w / v) sodium alginate solution, stir overnight, and mix the two solutions well; prepare 1% (w / v) sodium carboxymethyl cellulose solution, build a ...

Embodiment 2

[0041] Preparation of porous islet microcapsules with core-shell structure

[0042] (1) Design of microfluidic chip:

[0043] First design the microfluidic EFI chip, select capillary glass tubes with diameters of 800 microns and 300 microns, adjust the outlet of the external phase capillary to 500 microns by pulling the tube, and use the coaxial method to nest the capillary tubes with two diameters. The coaxial capillary is pasted and fixed with a needle at the corresponding place, leaving the inlet of the two-phase solution. Such as figure 1 shown. Repeated washing with ultrapure water to obtain a microfluidic chip.

[0044] (2) Preparation of porous islet microcapsules with core-shell structure:

[0045] In order to make the shell hydrogel have a porous structure, prepare polyethylene glycol diacrylate (PEGDA) solutions with different concentration ranges, add a small amount of 1% (w / v) PEO solution, stir overnight, and make the two solutions fully mix evenly; Prepare a...

Embodiment 3

[0047] Preparation of porous islet microcapsules with core-shell structure

[0048] (1) Design of microfluidic chip:

[0049]First design the microfluidic EFI chip, select capillary glass tubes with diameters of 800 microns and 300 microns, adjust the outlet of the external phase capillary to 500 microns by pulling the tube, and use the coaxial method to nest the capillary tubes with two diameters. The coaxial capillary is pasted and fixed with a needle at the corresponding place, leaving the inlet of the two-phase solution. Such as figure 1 shown. Repeated washing with ultrapure water to obtain a microfluidic chip.

[0050] (2) Preparation of porous islet microcapsules with core-shell structure

[0051] In order to make the shell hydrogel have a porous structure, prepare gelatin methacrylate (GelMA) solutions of different concentrations, add a small amount of 1% (w / v) PEO solution, stir overnight, and mix the two solutions well; prepare 1% (w / v) sodium carboxymethyl cell...

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Abstract

The invention discloses a preparation method and application of an aqueous two-phase porous pancreas islet microcapsule with a core-shell structure. According to the method, based on an electronic injection microfluidic system, a large number of microcapsules with liquid cores and hydrogel shells are obtained, a pore-forming agent polyoxyethylene (PEO) solution is added into an alginic acid (ALG) solution of the shells, so that the hydrogel of the shells becomes a porous structure; and the inner phase is a carboxymethyl cellulose (CMC) solution. The microcapsule has good biocompatibility; target cells are added into the inner phase solution to obtain the cell-loaded microcapsule; and due to the internal liquid environment and the external porous gel, the three-dimensional culture of the cells can be realized, the cell survival rate can be improved, and the cell-loaded microcapsule can be transplanted to realize the cell treatment effect. The preparation method of the core-shell structure porous pancreas islet microcapsule based on microfluidic electronic injection has the advantages of reusability, low cost, simplicity in operation, convenience, reliability and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, and specifically relates to a porous islet microcapsule based on microfluidic electrospray with a core-shell structure and a preparation method thereof. The porous islet microcapsule can be applied to cell transplantation to ensure cell activity and isolate immune rejection, etc. in the technical field. Background technique [0002] Commonly used methods of cell transplantation include hepatic portal vein, renal capsule, peritoneal cavity, etc. Microcapsules are one of the cell transplantation methods that can be transplanted into the peritoneal cavity. The microcapsule has a two-phase aqueous core-shell structure, and the shell layer is a porous hydrogel, which can allow small molecular substances to pass through and prevent macromolecular substances such as immune cells and immunoglobulins, and has a certain immune isolation effect; the inner phase Provide a 3D liquid environment for the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K47/36A61K47/10A61K47/38A61K47/42A61K35/39A61P3/10C12N5/071B01J13/02
CPCA61K9/5089A61K9/5036A61K9/5031A61K9/5057A61K47/38A61K35/39A61P3/10C12N5/0677B01J13/02C12N2513/00C12N2533/30C12N2533/74C12N2533/50C12N2533/54C12N2533/40
Inventor 李玲赵远锦魏琼刘小钰余筠如王月桐
Owner ZHONGDA HOSPITAL SOUTHEAST UNIV
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