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Lysable cell microcapsule and preparation method thereof and cell culture method

A technology for cell lysis and cell culture, which is used in cell culture such as cell adherent culture, lysable cell microcapsules and their preparation. The effect of developing and stabilizing proliferation

Active Publication Date: 2020-09-04
航天中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to at least solve the problem of the time limitation of cells before the launch of the aircraft in space experiments, and at the same time meet the needs of observing the fine structure of cells in space experiments, the present invention proposes a cell microcapsule that can be cleaved to realize the long-term maintenance of cells under unmanned control. nourish

Method used

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  • Lysable cell microcapsule and preparation method thereof and cell culture method
  • Lysable cell microcapsule and preparation method thereof and cell culture method

Examples

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preparation example Construction

[0033] In an exemplary microcapsule preparation method, it includes the following steps:

[0034] Prepare sodium alginate, calcium chloride, polylysine;

[0035] After dissolving sodium alginate in double distilled water, make sodium alginate solution, filter and sterilize for later use;

[0036] Calcium chloride is dissolved in double distilled water to make a calcium chloride solution, which is filtered and sterilized for subsequent use;

[0037] Dissolve polylysine in double distilled water to make polylysine solution, filter and sterilize for later use;

[0038] Add the cells to be cultured into the sodium alginate solution to make a mixed suspension of sodium alginate cells;

[0039] Adjust the density of cells and the concentration of sodium alginate in the mixed suspension;

[0040] The mixed suspension of sodium alginate cells was added to the calcium chloride solution and allowed to stand for 10 minutes to form stable calcium alginate beads.

[0041] Add calcium a...

Embodiment 1

[0051] 1. After dissolving sodium alginate in water, filter it with a 0.22 micron filter. Calcium chloride is formulated into a calcium chloride solution according to a mass volume ratio of 1.1%, and filtered with a 0.22 micron filter. The polylysine was formulated into a 0.05% concentration solution and filtered with a 0.22 micron filter.

[0052] 2. The cells were added to the sodium alginate solution to prepare a sodium alginate cell mixture, and the final concentration of sodium alginate in the sodium alginate cell mixture was controlled to be 1% (w / v).

[0053] 3. The sodium alginate cell suspension was dripped into the calcium chloride solution through a syringe using oxygen blowing pressure to condense into calcium alginate beads, let stand for 10 minutes, and then rinsed 3 times with sterile normal saline.

[0054] 4. Add calcium alginate beads into the polylysine solution, let it stand for 10 minutes, and then rinse it with sterile saline for several times.

[0055]...

Embodiment 2

[0057] This example is used to illustrate the process of adding sodium alginate dropwise using a microcapsule generating device.

[0058] The microcapsule generating device in this embodiment includes a syringe (1), a syringe needle (2), a sterile plastic blown pressurized tube (3), a connector (7) and an oxygen pipeline (6).

[0059] When in use, suck the cell sodium alginate solution with a syringe (1). Insert the syringe needle (2) from the side wall of the sterile plastic blown pressurized tube (3). The syringe needle (2) should protrude beyond the end of the sterile plastic blown pressurized tube (3). Connect the sterile plastic blown pressurized tube connector (7) to the oxygen line (6). The oxygen valve is opened, and the pressure is adjusted to 5pa, and it can be seen that eddy surges are generated in the calcium chloride solution in the petri dish (4). Pressurize and push the syringe (7) at a constant speed. When made, the resulting suspension should be dripped on...

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Abstract

The present invention discloses a lysable cell microcapsule and a preparation method thereof and a cell culture method. The lysable cell microcapsule comprises a capsule wall and cells and a culture solution wrapped by the capsule wall, the capsule wall can create an environment for long-term stable cell proliferation, and at the same time, after a certain period of time, the microcapsule is lysedto release the cells growing adherently to the wall to meet needs of cell morphology observation. The cell microcapsule is particularly suitable for cell experiments in a space environment conductedin space.

Description

technical field [0001] The present invention relates to the field of cell culture, in particular to cleavable cell microcapsules, a preparation method thereof and a cell culture method, especially a method for cell culture such as cell adherent culture in an outer space environment. Background technique [0002] With the development of the aerospace industry, there are more and more demands for space cell level research, but due to various constraints, only some of the research has been successfully implemented, and the preparation time before the launch of the aircraft is an important factor. Under the culture conditions of the ground environment, the cells can proliferate and cover the surface of the culture vessel after about 7 days. If they cannot be separated and passaged in time, and the culture environment is changed, the cells will age and die. In order to avoid cell proliferation covering the surface of the culture vessel before entering space, the cell loading time...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/096C12N11/04C12N5/071C12N5/075
CPCC12N11/10C12N11/096C12N11/04C12N5/0609C12N5/0682
Inventor 田永胜王艳平郭良蓉刘萍苏琳王丹
Owner 航天中心医院
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