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35 results about "Cleaved Cell" patented technology

Having to do with the appearance of cells when viewed under a microscope. The nucleus of cleaved cells appears divided or segmented.

Universal Anti-tag chimeric antigen receptor-expressing t cells and methods of treating cancer

The present invention provides a universal, yet adaptable, anti-tag chimeric antigen receptor (AT-CAR) system which provides T cells with the ability and specificity to recognize and kill target cells, such as tumor cells, that have been marked by tagged antibodies. As an example, αFITC-CAR-expressing T cells have been developed that specifically recognize various human cancer cells when those cells are bound by cancer-reactive FITC-labeled antibodies. The activation of αFITC-CAR-expressing T cells is shown to induce efficient target lysis, T cell proliferation, and cytokine / chemokine production. The system can be used to treating subjects having cancer.
Owner:UNIV OF MARYLAND BALTIMORE

Device and method for rapidly lysing cells or viruses

A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provide. Further, a method of lysing cells or viruses using the cell lysis device is provide. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.
Owner:SAMSUNG ELECTRONICS CO LTD

Application of antiplatelet thrombolysin in preparation of medicine for treating thrombotic thrombocytopenic purpura (TTP)

The invention relates to the field of medicines and especially relates to an application of antiplatelet thrombolysin in preparation of a medicine for treating thrombotic thrombocytopenic purpura (TTP). The antiplatelet thrombolysin limits adhesion and aggregation of platelets, recovers levels of the platelets, erythrocytes and hemoglobin, reduces level of lactic dehydrogenase and effectively inhibits thrombocytopenia and cleaved-cell hemolyticanemia, thereby treating the TTP. The antiplatelet thrombolysin has great clinical application prospect.
Owner:ZHAOKE PHARMA HEFEI

Method for extracting total DNAs of soil microorganisms at high purity

The invention discloses a method for extracting total DNAs of soil microorganisms at high purity. CTAB (Cetyltrimethyl Ammonium Bromide), lysozyme, protease and SDS (Sodium Dodecyl Sulfonate) act together to perform lysis of cells and simultaneously freeze and thaw the cells repeatedly; therefore, an excellent cell lysis effect is achieved; the humic acid is removed by utilizing PVP (Polyvinyl Pyrrolidone), CaC12 and BSA (Bull Serum Albumin); and the DNAs are precipitated by utilizing isopropanol so that the purity of the obtained DNAs is high. The method is simple and convenient; and without being purified, the DNAs obtained by the method can be used for subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (Denaturing Gradient Gel Electrophoresis) analysis. The purity of the extracted DNAs meets the requirement for directly performing subsequent molecular biological research; the extracted DNA segments can be subjected to 16SrDNA amplification and DGGE atlas analysis; glue cutting recovery and basic group sequencing can be performed on specific stripes of the DGGE band; unknown bacteria can be identified through comparison with the conventional sequences in an RDP (Ribosomal Database Project) database or by establishing a new sequence probe; and therefore, the structural composition of the microbial community can be determined, and a foundation can be laid for researching the structural diversity and ecological functions of the soil microorganisms in the rhizosphere of the mulberry in future.
Owner:INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI

FTA-coated media for use as a molecular diagnostic tool

There is provided a substrate for lysing cells and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.
Owner:GLOBAL LIFE SCI SOLUTIONS USA LLC

Method for extracting DNA from acidic mine water bed mud in coal mine

InactiveCN101648985ASolve the problem of low extraction efficiencySugar derivativesDNA preparationMicroorganismLysis
The invention relates to a method for extracting DNA from acidic mine water bed mud in a coal mine. Buffer solution elution, supersonic oscillation and membrane filtration for thallus collection are adopted in the method, and cell lysis is performed to extract DNA. The extracted DNA can be applied to such molecular biology technologies as PCR amplification, DGGE and TRFLP and the like for analyzing microbial communities in the acidic mine water bed mud in the coal mine without purification. The method has the advantages of being simple and rapid, and being capable of truly reflecting diversityof different microbial populations in the acidic mine water bed mud in the coal mine.
Owner:HENAN INST OF ENG

Kit for nucleic acid extraction with magnetic bead method, magnetic bead and preparation method of magnetic bead

The invention discloses a kit for nucleic acid extraction with a magnetic bead method, magnetic beads and a preparation method of the magnetic beads. The kit for nucleic acid extraction with the magnetic bead method comprises a cell lysis solution, the magnetic beads and a washing solution, wherein the cell lysis solution contains guanidine isothiocyanate, Triton X-100, 3-[3-(cholamidopropyl) dimethylamino]-1-propanesulfonic acid and N-sodium lauroyl sarcosinate. According to the kit for nucleic acid extraction with the magnetic bead method, the components of the lysis buffer solution are optimized, so that cells can be quickly and fully lysed, proteins can be quickly and fully denatured, the proteins and nucleic acid are effectively separated, and the nucleic acid is released; and the working efficiency of the cell lysis solution is improved, and the nucleic acid extraction time is shortened.
Owner:MGI TECH CO LTD

Device and method for rapidly lysing cells or viruses

A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provided. Further, a method of lysing cells or viruses using the cell lysis device is provided. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.
Owner:SAMSUNG ELECTRONICS CO LTD

Methods of extracting nucleic acids

InactiveUS20070190526A1Rapid captureRapid and simple extraction and isolationSugar derivativesMicrobiological testing/measurementSolid phasesLysis
Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
Owner:NEXGEN DIAGNOSTICS LLC

Method for extracting RNA from calcified tissue

The invention discloses a method for extracting RNA from a calcified tissue. The method comprises the following steps: grinding the calcified tissue in liquid nitrogen until the blocky particle diameter is less than 0.5cm, adding Trizol, then adding the liquid nitrogen to continue to grind into powder until the diameter is about 1mm or less than 1mm; then transferring the obtained powder into a RNase-Free tubule, adding the Trizol for cell digestion lysis; centrifuging the tubule, transferring a supernatant to a new tubular; adding chloroform into the supernatant for treatment, then centrifuging, transferring an topmost layer aqueous phase to another new tubular, adding a 75% ethanol solution, mixing gently for nucleic acid precipitation; then transferring to an adsorption column in a kit, standing then centrifuging for RNA to bind to the adsorption column; using a kit solution for washing; and then eluting to obtain the total calcified tissue RNA. The obtained total calcified tissue RNA has the advantages of high purity, high yield, good quality and good integrity.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION

Preservation method for preparing trace amount of cell lysate in batches, and application thereof

The present invention discloses a preservation method for preparing a trace amount of a cell lysate in batches. The preservation method comprises the following steps: (1) glycerol, DMSO or a mixture of the glycerol and DMSO are into a Trizol or TCL buffer lysate to be uniformly mixed; (2) the Trizol or TCL buffer lysate containing the additives is into a 96-well plate or a 384-well plate accordingto a certain volume to lyse cells or tissues, and a pipette is used for flowing and beating or other tools are used for uniform mixing; (3) the cell or tissue lysate well plates are rapidly placed ina refrigerator at a temperature of between -20 and 80 DEG C for preservation; (4) after stored for a period of time, the cell or tissue lysate well plates are taken out from the refrigerator, and thecell or tissue lysate is absorbed for RT-PCR after dissolution; and (5) electrophoresis detection is conducted. The method solves problems of crystallization and pollution by separately adding the glycerol in different proportions or the DMSO in different proportions or mixing the glycerol and DMSO in different proportions and then conducting adding.
Owner:HANGZHOU BIOLYNX TECH CO LTD

Silicon bead test nucleic acid extraction kit as well as use method and application thereof

The invention provides a silicon bead test nucleic acid extraction kit as well as a preparation method and application thereof. The silicon bead test nucleic acid extraction kit includes a lysis solution A, a lysis solution B, a proteinase K solution, an adsorption solution, an adsorption bead suspension, a rinsing solution and an eluent, wherein the lysis solution A, the lysis solution B and theproteinase K solution are mixed to form a lysate. After the lysis solution A, the lysis solution B and the proteinase K solution are mixed in proportion, the use volume of the lysate can be increased,complete immersion of a tested material is facilitated, so that nucleic acids in cells can be fully released; and at the same time, the problems of low lysis efficiency and decomposition in the presence of light caused by interaction between the lysis solution A and the lysis solution B can be solved, the lysate lyses the cells sufficiently and efficiently, the lysis efficiency is high, the nucleic acids with a higher concentration is obtained, improvement of the quality of the nucleic acids is facilitated, and accuracy of subsequent operation is ensured.
Owner:广州高盛生物科技有限公司

Method for measuring tyrosine kinase phosphorylation

InactiveUS7981633B2Quality improvementSpecificity efficientlyMicrobiological testing/measurementImmunoglobulinsAutophosphorylationO-phospho-L-tyrosine
The present invention relates to methods for measuring the autophosphorylation of one or more tyrosine kinases and use of such methods in profiling kinase inhibitors and activators. As a representative example, the method comprises inducing kinase autophosphorylation activity in cells in presence and in absence of a kinase inhibitor, lysing the cells, capturing the tyrosine kinase in the cell lysate by adding a plurality of tyrosine kinase specific binding proteins which are associated with unique dyes, adding a phosphotyrosine specific antibody tagged with a marker which is distinguishable from the unique dyes, and identifying the autophosphorylated tyrosine kinase by detecting the unique dye and the marker. Alternately, the tyrosine kinases themselves could be coupled to the unique dyes. The present invention also relates to kits and compositions for carrying out the above-described methods.
Owner:MERCK PATENT GMBH

Bispecific t-cell engager with cleavable cytokines for targeted immunotherapy

A long acting modified T-cell engager bispecific antibody with cytokine caps that provides reduced toxicity and boosted anti-tumor activity is disclosed. Method of making the modified and cytokine capped Bi-specific T-cell engager antibody is also disclosed.
Owner:SHENZHEN ENDURING BIOTECH LTD

Method for promoting recombinant virus vector to be secreted out of cells

The invention relates to preparation of a virus vector, and provides a method for promoting secretion of a recombinant virus vector to the outside of a cell. The technical scheme is as follows: in the process of cell culture or / and virus inoculation, a polysaccharide chemical substance containing sulfo groups is added into a culture medium to promote a virus vector to be secreted out of cells. The method specifically comprises the following steps: (1) cell resuscitation; (2) cell passage; (3) virus inoculation and culture; and (4) harvesting viruses. According to the method, a polysaccharide chemical substance containing a sulfo group is added in the virus amplification process, and the virus is promoted to be secreted from inside to outside of cells, so that in the virus harvesting process, cells do not need to be split, a culture supernatant is directly harvested to obtain the virus, and the harvesting process of a virus vector mainly located in the cells is simplified. The method provided by the invention is simple in process, harmful chemical substances such as a detergent are not introduced, the virus can be harvested without a complicated cell lysis step, and the method is economical and environment-friendly.
Owner:OBIO TECH SHANGHAI CORP LTD

A kind of preservation method and application thereof for batch preparation of micro-cell lysate

The present invention is a kind of preservation method that is used for preparing trace cell lysates in batches, comprises the following steps: (1) adding glycerol, DMSO or the mixture of both into Trizol or TCL buffer lysates and mixing; (2) using Add a certain volume of Trizol or TCL buffer lysate containing the above additives to a 96-well plate or a 384-well plate to lyse the cells or tissues, and mix them evenly with a pipette or other tools if necessary; (3) Quickly dissolve the cells or tissues The orifice of the lysate should be stored in a refrigerator at -20°C to 80°C; (4) After a period of storage, take the cell or tissue lysate out of the refrigerator, absorb the cell or tissue lysate for RT-PCR after dissolving ; (5) detected by electrophoresis. The method solves the problems of crystallization and contamination by adding different proportions of glycerin or DMSO separately, or mixing glycerin and DMSO in different proportions and then adding them.
Owner:HANGZHOU BIOLYNX TECH CO LTD

Method special for rapid massive extraction of oral epithelial cell DNA

The invention relates to a method special for rapid massive extraction of oral epithelial cell DNA, wherein the method comprises the following steps: (1), collecting sample cells; (2) cracking the cells; (3) removing proteins; (4) precipitating DNA; (5) washing with ethanol; (6) dissolving the DNA, and (7) preserving the DNA, marking the DNA sample, placing the DNA sample in a specified position at the temperature of -20 DEG C or -80 DEG C, and preserving. Compared with a centrifugal column method and a magnetic bead method, the method provided by the invention greatly reduces the experimentalcost; compared with the centrifugal column method and the magnetic bead method, the method has the DNA extraction time greatly shortened; compared with the centrifugal column method, the method greatly increases the amount of DNA extraction. The purity of the DNA is within a reasonable range. The extracted genomic DNA does not affect downstream molecular experiments.
Owner:TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD

Protocol for detecting interactions within one or more DNA molecules within a cell

The invention provides a method for detecting interactions between elements within one or more DNA molecules within a cell, wherein the elements are not adjacent in the primary DNA sequence, the method comprising: a) providing a cell in which elements within one or more DNA molecules that are in close proximity are cross-linked; b) simultaneously lysing the cell and mechanically fragmenting the DNA molecules within the cell; c) proximity ligating the one or more fragmented DNA molecules; d) reversing the crosslinks in the ligated DNA molecules; e) sequencing the ligated DNA molecules; and f) analysing the sequencing data to detect interactions between elements within the one or more DNA molecules within the cell.
Owner:OXFORD NANOPORE TECH LTD

Trace protein immunoblotting detection method

The invention particularly relates to a trace protein immunoblotting detection method, and belongs to the field of protein detection. The method is obtained by improving a traditional protein immunoblotting detection method, cells are directly lysed through a sample loading buffer solution, protein loss in the process that the cells are lysed through a lysate traditionally is avoided, the pore diameter of a gel making comb is adjusted to be 1.0-1.5 mm, the detection signal is enhanced, the defect that the total amount of protein donor cells required by the existing western blot technology is more than 10<6> is overcome, the method can be used for detecting the expression of a small amount of cell proteins, and the detection range of western blot is effectively expanded.
Owner:重庆生命知源科技有限公司

Cell lysate and application thereof for ATP detection

The invention belongs to the field of biotechnology, and discloses a cell lysate for ATP detection and an application thereof. The cell lyse is prepared by adding TritonX-100, EDTA, NaCl and the like to a buffer. The cell lysate of some examples of the present invention can lyse the cells and stabilize the ATP in it well at the same time, and the lysed samples can be stored stably at 2-8°C, which helps to obtain more accurate ATP detection As a result, storage conditions are easily satisfied. Compared with the existing cell lysate, the detection stability has been greatly improved, and it has the advantages of convenient operation, easy preparation, and low cost. The cell lysates of some examples of the present invention can be stably stored at 2-8°C, and the storage conditions are easy to meet, and repeated freezing and thawing are not required for use.
Owner:GUANGZHOU LDEBIO TECH CO LTD

A high-resolution conformation technique of genome-wide chromatin based on a small number of cells ehi-c 2.0

The invention discloses eHi-C 2.0, a high-resolution conformation technology based on a small number of cells and whole genome chromatin. The invention provides a preparation method of a cell eHi-C 2.0 sequencing library: lyse cells to obtain chromatin, and then digest The chromatin; the digested DNA is sequentially labeled with a marker and blunt-ended to obtain a circularized product; introducing an internal reference circular co-precipitated DNA molecule into the circularized product; and then using a restriction exonuclease Enzyme digestion, then ultrasonic fragmentation, and then use immunomagnetic beads to grab the DNA fragments with the markers from the fragmentation products; use the DNA fragments with the markers to prepare eHi-C 2.0 sequencing library . The present invention can perform eHi-C library construction and sequencing on the number of cells as low as 0.1 million cells.
Owner:AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI

Lysable cell microcapsule and preparation method thereof and cell culture method

ActiveCN111621493AImprove permeabilityImprove the efficiency of culture expansionArtificial cell constructsOn/in organic carrierCells/microLCell biology
The present invention discloses a lysable cell microcapsule and a preparation method thereof and a cell culture method. The lysable cell microcapsule comprises a capsule wall and cells and a culture solution wrapped by the capsule wall, the capsule wall can create an environment for long-term stable cell proliferation, and at the same time, after a certain period of time, the microcapsule is lysedto release the cells growing adherently to the wall to meet needs of cell morphology observation. The cell microcapsule is particularly suitable for cell experiments in a space environment conductedin space.
Owner:航天中心医院

A blood cell lysate and method for extracting nucleic acid in blood using the lysate

The invention discloses a blood cell lysate and a method for extracting nucleic acid in blood by using the lysate. The blood cell lyse contains heparin magnetic beads with a mass fraction of 3% to 10%, and Triton X-100 with a mass fraction of 0.5 to 1.5%. , urea with a final concentration of 0.5-1.0M, mass fraction of 0.02-0.08% sodium lauroyl sarcosinate, and calcium chloride and / or magnesium chloride with a final concentration of 0.1-0.3M. The compound detergent in the lysate of the present invention can effectively lyse cells, and the heparin magnetic beads can effectively adsorb proteins, and under the action of calcium (magnesium) ions, PCR interfering substances such as proteins can be solidified rapidly and nucleic acids can be separated out by warm treatment. Nucleic acids of high purity are obtained by centrifugation. The lysate of the present invention is stable in nature and temperature, and is not affected by seasons, temperatures, salt ion concentrations, etc., and the method is simple and fast in operation, is not easy to be polluted, has a high yield of nucleic acid extraction, and is suitable for clinical and scientific research.
Owner:BEIJING NAGENE DIAGNOSTIC REAGENT CO LTD

Method for rapidly identifying shRNA effectiveness

The invention discloses a method for rapidly identifying shRNA effectiveness. The method comprises the following steps: (1) designing and synthesizing a plurality of candidate shRNAs as candidate functional shRNAs; (2) constructing a PLKO.1-shRNA recombinant vector based on a lentiviral vector; (3) in HEK293 / HEK293T cells, co-transfecting the HEK293 / HEK293T cells into the cells by using a cell transient transfection method; (4) after the transfection is finished for 6-8 hours, replacing a fresh culture medium for the cells and continuously culturing for 24-48 hours; (5) collecting the cells, cracking the cells, and extracting total protein; and (6) detecting the expression of a target protein by Western Blot, and identifying the effectiveness of the candidate shRNAs by comparing the expression difference of target proteins in a control group and a treatment group. According to the method, the time cost is saved by about 50%, the overall experimental workload is reduced by about 70%, and the labor cost is remarkably and greatly reduced.
Owner:SHENZHEN UNIV

Cleasable cell microcapsules and its preparation method and cell culture method

The invention discloses cleavable cell microcapsules, a preparation method and a cell culture method thereof. The lysable cell microcapsules of the present invention include a capsule wall and cells wrapped by the capsule wall and culture fluid. The capsule wall can create an environment for long-term stable proliferation of cells. The cells grow adherently to meet the needs of cell morphology observation. The cell microcapsule of the present invention is particularly suitable for cell experiments in space environment.
Owner:AEROSPACE CENT HOSPITAL
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