Two-step microcapsule preparation method in bio-artificial reaction device

Abstract

The invention relates to a two-step microcapsule preparation method in a bio-artificial reaction device. The invention adopts the technical scheme that biological cell material is fully and evenly mixed in 2 percents of sodium alginate solution, the obtained suspension enters a microcapsule generator from a jet microcapsule generator and then is jetted into calcium chloride solution to react poly-lysine solution and then react with sodium alginate solution, and microcapsules are obtained; and the microcapsules are fully and evenly mixed with sodium alginate solution; the above step is carried out again through the jet microcapsule generator; and microcapsules are obtained after microcapsule cores are liquefied by sodium citrate. The invention overcomes the defect in the prior microcapsules that some cell materials are embedded among capsule membranes of the microcapsules to cause the attack of immune molecules resulting in the damage of the tightness of the microcapsule. The microcapsule produced with the two-step microcapsule preparation method has stronger mechanical tension, still can keep the integrity of the microcapsule walls under the impact of high shear force, and reduces the overflow ratio of cell materials, and the immune insulation function prevents the integrity of the microcapsule walls from being damaged.

Description

technical field [0001] The invention relates to a bioartificial reaction device, in particular to a two-step microcapsule preparation method in the bioartificial reaction device. Background technique [0002] Bioartificial reaction device is a new type of artificial support system, which has broad application prospects. The basic principle is to place the biological cell material cultured and proliferated in vitro in a circulation device, and the patient's blood / body fluid exchanges material with the cultured biological cell material through the biological fiber semi-permeable membrane, so as to achieve the ideal artificial support function. The reaction device is the core part of the bioartificial reaction system. At present, clinical and scientific research adopts a coated suspension external bioartificial reaction device. It uses non-toxic polymer membrane materials to wrap cell materials to form a diameter of tens of microns to Microcapsules with hundreds of microns sep...

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Problems solved by technology

[0003] Before the present invention, the microcapsules used in the current bioartificial reaction device all adopt the one-step microcapsule method to make, such as figure 2 As shown, the biggest defect of the microcapsules prepared by this method is that it is easy to cause some cell materials to be embedded between the capsules of the microcapsules, thereby causing the attack of immune molecules, destroying the sealing property of the microcapsules, and finally causing Fibrosis occurs and the function of the cell material in the microcapsule decreases; secondly, the integrity and airtightness of the microcapsule prepared by the one-step microcapsule method is damaged due to the incarceration of the cell material, resulting in poor mechanical strength. The microcapsules in the state of high perfusion cannot withstand the high shear force brought by the body fluid circulation, which can easily cause the rupture of the capsule membrane, resulting in the overflow of cell materials; finally, because the mechanical strength of the microcapsule membrane is not high, the microcapsule After cryopreservation, especially liquid nitrogen cryopreservation and recovery, the integrity of the microcapsule wall is reduced, and the activity of biological cell materials cannot be effectively maintained

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