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Method for culturing luminous pearls with pinctada martensi

A technology for pearl oyster martensii and pearls, which is applied in the field of aquaculture, and can solve the problems of no strict requirements and changes in the culture environment, pearl purity and yield impact, etc., so as to improve pearl quality, enhance promotion and application value, and increase yield and high-quality effects

Inactive Publication Date: 2019-02-01
贺琪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no strict requirements and changes to the culture environment, which will have a great impact on the purity and yield of the cultivated pearls

Method used

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  • Method for culturing luminous pearls with pinctada martensi

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Effect test

Embodiment 1

[0032] Embodiment one, such as figure 1 As shown, a method for cultivating luminous pearls of Pinctada martensii according to an embodiment of the present invention comprises the following steps:

[0033] Step S101: sea area selection: the sea area is selected in a tropical or subtropical monsoon climate sea area with high salinity, large evaporation, good water quality, and no pollution. All indicators of cadmium, lead, arsenic, mercury, and PCBs in the sea area should be Conforms to the national ocean quality first-class standard;

[0034] Step S103: Select nucleus insertion: prepare digestive juice by trypsin, dissociate the required lamella, select lamella with small mantle cells, and the closer the shell color of the lamella is to white, the better;

[0035] Step S105: Cultivate pearl sacs: apply fibronectin, polylysine, rat tail collagen, and SM substances on the luminous bead nucleus, dry and sterilize with high-pressure steam, and suck the cell suspension of the mantl...

Embodiment 2

[0042] Example 2, the trypsin is used to prepare digestive juice, the concentration is 0.3%, the pH value is 7.2-7.4, and the digestion temperature is 25°C.

Embodiment 3

[0043] Embodiment 3, the sodium chloride in the digestive juice can be replaced by sodium gluconate.

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Abstract

The invention discloses a method for culturing luminous pearls with pinctada martensi. The method for culturing the luminous pearls with pinctada martensi includes the following steps of selecting waters, wherein the waters with tropical or subtropical monsoon climates are selected, the waters are high in seawater salinity, large in evaporation capacity, good in water quality and free of pollution, and indexes of cadmium, lead, arsenic, mercury and polychlorinated biphenyl in the waters all accord with national marine quality first-class standards; selecting insertion nuclei, wherein digestionliquid is prepared with trypsin, required small piece shells are dissociated, small piece shells with mantle cell pieces are selected, and the small piece shells had better be white; culturing pearlsacs, wherein luminous pearl nuclei are painted with fibronectin, poly-lysine, rat tail collagen and an SM substance, high-pressure steam sterilization is conducted after drying, cell suspension of mantle tissue is sucked and dropped on the luminous pearl nuclei, mantle tissue blocks are attached to the luminous pearl nuclei, and then a medium is sucked and dropped to conduct culturing for three days. The method for culturing the luminous pearls with pinctada martensi has the advantages of increasing the yield, improving the quality of the pearls and making the pearl industry actually achievehigh yield and high quality.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for cultivating luminous pearls from Pinctada martensii. Background technique [0002] Breeding Pinctada martensii to cultivate seawater pearls is a green industry that benefits the country and the people. The current survival rate of seawater pearls needs to be improved. important way. [0003] The existing authorized patent No. CN201510259406.6 discloses a method for cultivating luminous pearls from Pinctada martensii, including the following steps: Step 1, grinding the pearl core from Pinctada martensii shells, The outside of the core is sequentially wrapped with nano-silver, luminescent sub-layer, and cold water sol layer to prepare a luminescent bead core with a diameter of 3 mm; step 2, inserting the 3 mm luminescent bead core to obtain pearl oysters; step 3, cultivating pearl oysters shellfish to obtain luminous pearls; the method for luminous pearls cultiva...

Claims

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Application Information

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IPC IPC(8): A01K61/56
CPCA01K61/56Y02A40/81
Inventor 贺琪
Owner 贺琪
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