9 results about "Pinctada martensii" patented technology

The present application relates to the technical field of polypeptide, and discloses a pearl producing Pinctada martensii with whitening and antioxidant effects, a preparation method and application thereof, wherein the pearl producing Pinctada martensii is a mixture of short peptides, the pearl producing Pinctada martensii takes pearl layer powder of Pinctada martensii as raw material, water and composite ionic liquid are added in proportion, and after physical modification under high temperature and high pressure, the pearl producing Pinctada martensii is sequentially subjected to complex degradation by bromelain and central protease, enzyme inactivation, centrifugation, separation and purification, and identification. The pearl producing Pinctada martensii prepared by the present application has the effects of whitening and free radical scavenging, and the present application provides a new type of bioactive peptide for the field of cosmetics, and simultaneously improves the application value of pearl layer powder in the fields of biological medicine and food.

The present application relates to the technical field of wound repair, and provides a traditional Chinese medicine composition for promoting wound healing and a preparation method thereof.The traditional Chinese medicine composition comprises a first traditional Chinese medicine composition and a second traditional Chinese medicine composition which are applied to the acute phase and the repair proliferation phase.The former comprises naked flower purple perilla extract, nectarine leaf extract, purple perilla extract, dragon's blood micro powder, spironast extract, centella asiatica volatile oil, rhubarb extract, borneol, mussel mucin, medicinal glycerol and purified water.The latter comprises red algae extract, nectarine leaf extract, radix astragali extract, panax notoginseng total saponins, aloe extract, dendrobium officinale extract, sea buckthorn extract, pinctada martensii purified peptide, snail glycosaminoglycan, medicinal glycerol and purified water.The composition can play the roles of antibiosis, anti-inflammation, hemostasis, analgesia and astringency in the acute phase, and can promote angiogenesis, collagen synthesis and epithelial regeneration in the repair proliferation phase, so that the wound is rapidly repaired and healed.

The present invention discloses a Pinctada martensii C-type lectin PmPLP, as well as its application in the preparation of drugs for inhibiting microorganisms and in promoting the release of pro-inflammatory factors induced by nucleation in Pinctada martensii. The present invention conducts preliminary research on the identification and immune function of Pinctada martensii C-type lectin PmPLP to determine the immune response of PmPLP molecules in the immune response of Pinctada martensii against the invasion of external pathogens, and to understand the antibacterial activity of PmPLP after in vitro expression, providing data support and research basis for the study of the immune defense mechanism of Pinctada martensii and other marine mollusks C-type lectins. The present invention conducts in-depth research on the immune response mechanism of Pinctada martensii, which helps to reveal its molecular regulatory mechanism in response to pathogen invasion, and is of great significance for improving aquaculture efficiency and disease prevention and control.

The invention belongs to the technical field of marine ecological protection and aquaculture, and discloses an intervention method for relieving acid etching damage of shellfish larvae, according to the method, by adding strontium ions (Sr) with different concentrations into seawater, the malformation rate of the shellfish larvae under the acidification condition can be effectively reduced, the shell length, the mechanical property and the calcified gene expression level can be improved, and the acid etching damage of the shellfish larvae can be relieved. Therefore, the embryo shell forming ability and the acid corrosion resistance are enhanced; according to the invention, Ruditapes philippinarum, Crassostrea sikamea and Pinctada martensii are used as representatives, strontium intervention experiments are respectively carried out in seawater with pH values of 8.1, 7.7 and 7.4, and results show that a proper amount of strontium can significantly enhance the hardness and toughness of shells, and a more stable Sr-Ca eutectic structure is formed by partially replacing Ca in the shells, so that the stress resistance of larvae is improved. The method is quick in effect, wide in application range and suitable for embryo shell protection and cultivation stress resistance enhancement in the cultivation process of various shellfish seedlings.

The application provides a SNP molecular marker related to low-temperature resistance of Pinctada martensii and application thereof, and belongs to the technical field of aquatic genetic breeding. The molecular marker comprises one or more of SNP351, SNP352, SNP362, SNP365, SNP367, SNP378, SNP396, SNP456, SNP718, SNP823 and SNP831. The molecular marker can be used for genotype identification of parent shellfish before seed breeding, and the low-temperature resistance of offspring population can be improved by screening parent shellfish with multiple advantageous genotypes.

This invention discloses a molecular marker array, microarray, and its applications for Pinctada martensii. The molecular marker array comprises 31,053 molecular markers, the physical locations of which were determined by sequence alignment based on the Pinctada martensii reference genome CNP0002248. The SNP sites in the Pinctada martensii molecular marker array of this invention are highly representative, polymorphic, and versatile. Furthermore, it covers sites and functional sites significantly associated with traits such as shell length, shell width, shell height, shell weight, shell color, pearl color, and nacre thickness. It can be used for gene identification and functional analysis of important economic traits in Pinctada martensii, research on population genetic diversity, germplasm resource conservation, genetic improvement, and marker-assisted breeding, to cultivate new varieties with excellent economic traits and promote the healthy and sustainable development of the marine pearl industry.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.

The present application relates to the technical field of shellfish culture, and particularly relates to a method for improving the low-oxygen tolerance of Pinctada martensii. The present application selects suitable Pinctada martensii for low-oxygen training, and then selects Pinctada martensii with mature gonad development after low-oxygen training as parent shellfish to breed seedlings; extracts nutrient solution from the Pinctada martensii after low-oxygen training; feeds the nutrient solution to Pinctada martensii larvae and juvenile shellfish for nutritional strengthening cultivation in the seedling stage, and adds the nutrient solution to strengthen the nutrition of algae in the culture of unicellular algae. The method provided by the present application can enhance the physical fitness of Pinctada martensii, protect the body tissue, reduce the stress response of Pinctada martensii to low oxygen, and can significantly improve the long-term low-oxygen tolerance of Pinctada martensii.

The invention discloses a Pinctada martensii C-type lectin PmPLP, an application of the Pinctada martensii C-type lectin PmPLP in preparation of drugs for inhibiting microorganisms and an application of the Pinctada martensii C-type lectin PmPLP in promotion of release of pro-inflammatory factors induced by nucleus implantation of Pinctada martensii. According to the invention, preliminary research is carried out on identification and immune functions of Pinctada martensii C-type lectin PmPLP, so that immune response of PmPLP molecules in immune response of Pinctada martensii for resisting invasion of external pathogenic bacteria is determined, and antibacterial activity of PmPLP after in-vitro expression is known; and data support and a research basis are provided for researching C-type lectin immune defense mechanisms of pinctada martensii and other marine mollusks. The immune response mechanism of the pinctada martensii is deeply studied, the molecular regulation mechanism of the pinctada martensii for coping with pathogen invasion is disclosed, and the method has important significance for improving the breeding efficiency and preventing and controlling diseases.