This invention discloses a method for
interspecific hybridization and
seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and
drying them, placing them in
seawater, continuously aerating and artificially circulating the water to induce natural
sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding
ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a
sperm-containing
water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a
seedling pond; and cultivating the fertilized eggs in
seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and
seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the
reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new
hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine
pearl oyster germplasm and industrial upgrading.