5 results about "Pinctada martensii" patented technology

The present application relates to the technical field of wound repair, and provides a traditional Chinese medicine composition for promoting wound healing and a preparation method thereof.The traditional Chinese medicine composition comprises a first traditional Chinese medicine composition and a second traditional Chinese medicine composition which are applied to the acute phase and the repair proliferation phase.The former comprises naked flower purple perilla extract, nectarine leaf extract, purple perilla extract, dragon's blood micro powder, spironast extract, centella asiatica volatile oil, rhubarb extract, borneol, mussel mucin, medicinal glycerol and purified water.The latter comprises red algae extract, nectarine leaf extract, radix astragali extract, panax notoginseng total saponins, aloe extract, dendrobium officinale extract, sea buckthorn extract, pinctada martensii purified peptide, snail glycosaminoglycan, medicinal glycerol and purified water.The composition can play the roles of antibiosis, anti-inflammation, hemostasis, analgesia and astringency in the acute phase, and can promote angiogenesis, collagen synthesis and epithelial regeneration in the repair proliferation phase, so that the wound is rapidly repaired and healed.

The invention belongs to the technical field of marine ecological protection and aquaculture, and discloses an intervention method for relieving acid etching damage of shellfish larvae, according to the method, by adding strontium ions (Sr) with different concentrations into seawater, the malformation rate of the shellfish larvae under the acidification condition can be effectively reduced, the shell length, the mechanical property and the calcified gene expression level can be improved, and the acid etching damage of the shellfish larvae can be relieved. Therefore, the embryo shell forming ability and the acid corrosion resistance are enhanced; according to the invention, Ruditapes philippinarum, Crassostrea sikamea and Pinctada martensii are used as representatives, strontium intervention experiments are respectively carried out in seawater with pH values of 8.1, 7.7 and 7.4, and results show that a proper amount of strontium can significantly enhance the hardness and toughness of shells, and a more stable Sr-Ca eutectic structure is formed by partially replacing Ca in the shells, so that the stress resistance of larvae is improved. The method is quick in effect, wide in application range and suitable for embryo shell protection and cultivation stress resistance enhancement in the cultivation process of various shellfish seedlings.

The application provides a SNP molecular marker related to low-temperature resistance of Pinctada martensii and application thereof, and belongs to the technical field of aquatic genetic breeding. The molecular marker comprises one or more of SNP351, SNP352, SNP362, SNP365, SNP367, SNP378, SNP396, SNP456, SNP718, SNP823 and SNP831. The molecular marker can be used for genotype identification of parent shellfish before seed breeding, and the low-temperature resistance of offspring population can be improved by screening parent shellfish with multiple advantageous genotypes.

This invention discloses a molecular marker array, microarray, and its applications for Pinctada martensii. The molecular marker array comprises 31,053 molecular markers, the physical locations of which were determined by sequence alignment based on the Pinctada martensii reference genome CNP0002248. The SNP sites in the Pinctada martensii molecular marker array of this invention are highly representative, polymorphic, and versatile. Furthermore, it covers sites and functional sites significantly associated with traits such as shell length, shell width, shell height, shell weight, shell color, pearl color, and nacre thickness. It can be used for gene identification and functional analysis of important economic traits in Pinctada martensii, research on population genetic diversity, germplasm resource conservation, genetic improvement, and marker-assisted breeding, to cultivate new varieties with excellent economic traits and promote the healthy and sustainable development of the marine pearl industry.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.