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Method for making seed of Pinctada martensii of red shell strain

A technology of pinnacle martensii and red shell color, which is applied in medical science, climate change adaptation, animal delivery, etc., can solve problems such as the unseen seed production method of pinnacle martensii, and achieve the effect of improving purity

Inactive Publication Date: 2006-09-06
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the seed production method of the red-shelled strain of Pinctada martensii

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Seed production

[0037] a. Broodling

[0038] From a family (breeding group) with red shell color, select individuals with obvious red shell characteristics and full gonads, and the shellfish age is 2 years old; they are kept indoors for 5 days, and the water temperature is above 25°C, and they are cultured at room temperature. During the breeding period, the water is changed once a day, and sufficient bait is fed to promote the further development of the gonads into a physiologically mature state.

[0039] Sperm and egg collection and insemination

[0040] Sperm and eggs were collected by dissection. The insemination adopts single crossing, and the sperm and eggs are respectively drawn into a beaker filled with filtered sea water. The collected sperm and eggs are filtered through a sieve to remove impurities and tissue fragments. And quickly add the eggs into a flat-bottomed glass container filled with 0.005% ammonia seawater, stay for 10 minutes, then add an a...

Embodiment 2

[0049] Place: Daya Bay, Shenzhen, time: October 2004, several generations were cultivated in the manner of Example 1, and when the red shell ratio reached more than 90%, the multiplication and production of seedlings began: each single-breeding group was paired for group mating, and the red shell was taken. There were 4 female shellfish and 4 male shellfish. Sperm eggs were collected by dissection, and the eggs were treated with 0.006% ammonia seawater for 10 minutes, then sperm were added to inseminate at a water temperature of 23°C, and then the water was changed twice with filtered seawater. Conventional breeding results in a red shell ratio of 80%.

Embodiment 3

[0051] Location: Daya Bay, Shenzhen, time: April 2005, similar to Example 2, take 1 female shellfish and 1 male shellfish with red shell, collect sperm eggs by dissection, treat the eggs with 0.006% ammonia seawater for 10 minutes, and then Add sperm to inseminate at a water temperature of 24°C, and then change the water twice with filtered seawater. Conventional breeding results in a red shell ratio of 96%.

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PUM

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Abstract

The invention relates to a method for producing Malpighian mother-of-pearl in red shell color, comprising the following steps: (1) seed preparing: choosing out one female and one male shell with apparent red shell color from bred family with high purity of shell color or from wild or breeding group, single mating and culturing with every single mating group; (2) expanding breeding: breeding for several generations with methods mentioned above, expanding breeding after the red shell proportion is over 90%, matching every single mated group for group mating or mating the female plant of single mated group with male plant from wild or breeding group. The seed of expanded breeding is used as cell shell and pearl-breeding shell for red shell pearl cultivation. The invention can prepare pearl of red shell color with relatively simple process, the purity of red shell is increased to 96% in maximum, and there is no need of external gene through trans-gene and other complicate technique, for which the product is safe for biology and environment.

Description

technical field [0001] The invention relates to a seed production method of a red shell color strain Pinctada martensii Background technique [0002] Studies have shown that there is a certain correlation between the individual's choice of shell color in the growth process and its growth environment (including the temperature and salinity of the water area, etc.), geographical location and physiological factors. According to some shellfish research reports, shell color polymorphism is a simple Mendelian inheritance, but there is no clear conclusion whether the selection of shell color is necessarily related to growth traits, physiological traits and some economic traits. [0003] Pinctada martensii Dunker (Pinctada martensii Dunker) belongs to Bivalvia, Pearl Oysteraceae, and Pinctada genus. It is an important cultured seawater pearl shellfish. Its shell surface is light yellow, often with several dark brown radial lines. Other case colors are less common. During the resea...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61D19/00
CPCY02A40/81
Inventor 何毛贤管云雁袁涛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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