71 results about "Corbicula fluminea" patented technology

The invention discloses a shellfish flavor peptide and a preparation method thereof. The method comprises the following steps: using pinctada martensii and Corbicula fluminea as raw materials, and beating; rinsing by using a NaCl solution and filtering to obtain protein; conducting glycosylation: dispersing the protein into the NaCl solution to let the protein uniformly mix with glucan in a proper weight ratio, then conducting freeze drying and dry heating to obtain glucan shellfish protein; conducting enzymatic hydrolysis: adding water in the glucan shellfish protein, adjusting the pH value and the temperature, adding papain and composite protease to conduct enzymatic hydrolysis, after completing the enzymatic hydrolysis, killing enzyme to obtain an enzymoiysis product; carrying out centrifugal filtration on the enzymoiysis product to obtain an enzymatic hydrolysis solution; fermenting to remove fishy smell: carrying out autoclaved sterilization on the enzymatic hydrolysis solution, cooling, inoculating lactic acid bacteria, fermenting to obtain a broth; carrying out ultrafiltration on the broth by using a 5000Da ultrafilter membrane and drying to obtain the flavor peptide. The shellfish flavor peptide prepared by the invention has rich nutrients on the basis of keeping the original taste and guarantees the food safety.

The invention relates to a quasi-ecological corbicula fluminea surface-flow constructed wetland and an operating method thereof. The constructed wetland comprises a bed body, timed aeration devices, corbicula fluminea cages, corbicula fluminea, a sandy soil layer and wetland plants, wherein the wetland bed body is of a cuboid, a water inlet pipe and a water outlet pipe are respectively arranged at the front and rear ends of the wetland, the wetland bed body is made of concrete, the sandy soil layer with the thickness of 50-70cm is packed at the bottom of the bed body, the timed aeration devices are mounted on two sidewalls of the bed body, and the corbicula fluminea cages containing corbicula fluminea are placed in gaps among the wetland plants on the sandy soil layer. According to the quasi-ecological corbicula fluminea surface-flow constructed wetland and the operating method thereof, the corbicula fluminea cages, the plants and microorganisms are combined, so that sewage is subjected to intensified treatment, the quality of outlet water is good, and no secondary pollution is caused; compared with the natural state, the number of corbicula fluminea in water is increased greatly, and the sewage purifying effect is enhanced.

The invention provides a production process for corbicula fluminea solid beverage, which is characterized by obtaining a concentrated filtrate by sand removing the corbicula fluminea, cleaning, shell removing, paste forming, steaming and concentrating, filtering; charging a prolease with weight percentage lower than 10% into the filter residue after filtration, enzymolyzing for 20-60 minutes in the microwave radiation enzyme reactor, boiling for enzyme-eliminating, filtering to obtain the filtrate; mixing the two filtrate, adding auxiliary material and mixing, homogenizing and drying under the pressure of 60-80mPa to obtain the corbicula fluminea solid beverage. Applying the process, a large amount of natural water-soluble flavor substance is preserved, nutrient content is further developed, enzymolysis period is largely reduced, so that the product is high in solubility, abundant in nutrient, convenient for eating, and process is simple and suitable for industrial production.

The invention discloses a novel method for combined usage of Corbicula fluminea and Limnodrilus hoffmeisteri in inhibition of release of endogenous phosphorus in a closed water body. The method comprises the following steps: 1) adding Limnodrilus hoffmeisteri into sediment; 2) adding Corbicula fluminea in 2 to 3 days; and 3) detecting the concentration of phosphorus in water in each ten days until the concentration of phosphorus is stable. According to the invention, resources are saved, new chemical risks are prevented from being introduced into the water body, the purpose of control of release of endogenous phosphorus is realized, and the landscape function of the water body is prevented from being destroyed in the process of restoration of the water body.

The invention discloses a making method of instant corbicula fluminea. The making method is characterized by comprising the steps that pollution-free fresh corbicula fluminea is selected, cultured temporarily, cleaned, cooked and shelled, and complete corbicula fluminea meat is obtained; the corbicula fluminea meat serves as the raw material, and is finished, cleaned, drained, pickled to become tasty, dewatered, baked at a high temperature for sterilization, packaged and subjected to secondary sterilization, and the instant corbicula fluminea is obtained. The corbicula fluminea instant product maintains the integrity of the appearance and nutritional ingredients of corbicula fluminea meat and can be eaten as long as packages are opened; compared with the prior art, the method is easy to implement and reasonable in procedure, the processed corbicula fluminea product is free of nutrition losses, capable of being eaten as long as the packages are opened, good in color, delicious in taste and convenient to eat, and the eating convenience of corbicula fluminea is improved.

The invention provides a formula of a Corbicula fluminea shell juice composite seasoning. The formula is characterized in that: it involves the following materials by weight: 9.5% of Corbicula fluminea juice, 64.86% of water, 6% of white sugar, 8.3% of salt, 3.4% of a hydrolyzed vegetable protein liquid, 2.3% of monosodium glutamate, 0.04% of potassium sorbate, 0.4% of xanthan gum, 0.2% of citric acid, and 5% of mature ginger juice. The formula and the method provided by the invention are mainly directed at the problem that in traditional Corbicula fluminea processing processes, the Corbicula fluminea juice is not well utilized, so that the nutritional value of Corbicula fluminea lacks depth development.

The invention discloses a preparation method of Corbicula fluminea seasoning. The method comprises steps as follows: after Corbicula fluminea meat paste and water are mixed in the mass ratio being 1:1, mixed enzymes accounting for 2% of a raw material liquid are added, the mixture is subjected to water bath at the temperature of 60 DEG C for 6 h and then subjected to boiling water bath at 100 DEG C for enzyme denaturalixation for 15 min after enzymolysis is completed, centrifugation is performed, and an enzymatic hydrolysate is obtained; 10 mL of the enzymatic hydrolysate obtained in S1 is taken, 0.6 g of glucose, 0.02 g of xylose, 0.2 g of arginine, 0.1 g of glycine and 0.2 g of taurine are added, the mixture reacts at 100 DEG C for 1 h, the reaction pH is 6, a reaction liquid is freeze-dried, and the Corbicula fluminea seasoning is obtained. Through a Maillard reaction, the purpose of fishy smell removal can be achieved, and the aroma-enhancing effect can be realized, so that the seasoning with seafood and meat fragrance is obtained. The Corbicula fluminea seasoning has good flavor and taste and is very healthy and beneficial to a human body. The variety of seasoning in the market at present is greatly enriched, and even, a new market is developed.

The invention discloses a corbicula fluminea anti-oxidative peptide and a preparation method thereof. The corbicula fluminea anti-oxidative peptide is prepared by deeply developing corbicula fluminea meat with a compound enzyme microwave synergistic controllable enzymolysis technology, is novel and has an anti-oxidative effect. The utilization rate of corbicula fluminea resources is improved, a new path is provided for deep processing of corbicula fluminea, the additional value of the corbicula fluminea is promoted, the income of peasants is increased, and meanwhile, better social and economic benefits can be gained on the aspect of promoting the development of the corbicula fluminea aquaculture industry.

The invention provides a simulation method for negative effects caused by controlling juvenile fish outbreak by using filter feeding organisms to control an ecological rehabilitation project. The simulation method comprises the following steps: preparing sediment, preparing lake water, adding corbicula fluminea and carps to start an experiment and sampling, wherein the density of the added corbicula fluminea and carps is corbicula fluminea density treatment (0, 60 and 180) and is converted into the unit area biomass (namely 0, 214g/m<2> and 643g/m<2>); the density of the carps is (1 and 3) and is converted into the unit area biomass (namely 4.3g/m<2> and 12.9g/m<2>). According to an experiment biological method adopted by a biological principle, the simulation of the negative effects caused by controlling the juvenile fish outbreak by using the filter feeding organisms to control the ecological rehabilitation project is accurately realized; a biological operation principle is utilized and the filter feeding organisms, namely the corbicula fluminea, are added; the sediment suspension caused by the juvenile fish outbreak of the carps is controlled by the corbicula fluminea, and the illumination conditions of a water body are improved, so that the aim of repairing the eutrophication water body is realized; the method has the characteristics of safety, low cost and the like.

The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bath; 3) conducting enzyme deactivation after enzymolysis; 4) carrying out centrifugal treatment to remove precipitate so as to obtain supernatant, i.e. Corbicula fluminea enzymolysis liquid; 5) subjecting the Corbicula fluminea enzymolysis liquid to vacuum freeze drying so as to obtain Corbicula fluminea polypeptide powder; 6) preparing a Corbicula fluminea polypeptide solution; 7) adding zinc vitriol and the Corbicula fluminea polypeptide solution (with a pH value of 5.06-6) to perform chelation; 8) carrying out two-stage chelation reaction; 9) then conducting chelation reaction; 10) removing the supernatant and collecting the precipitate; and 11) conducting vacuum freeze drying to obtain Corbicula fluminea polypeptide zinc chelate powder. The method provided by the invention can achieve good chelating effect, has high chelating rate up to 81% or more, the oxidation resistance of Corbicula fluminea polypeptide is significantly improved after chelating with zinc, and compared with polypeptide, the chelate has better bioactivity.

Complete equipment for investigation of corbicula fluminea resources comprises an image device and a fishing device, and is characterized in that the image device comprises a waterproof camera (1), an image transmission cable take-up and pay-off device (2) and an image display terminal (3); one end of an image transmission cable is connected with the waterproof camera (1); the other end of the image transmission cable is connected with the image display terminal (3); the waterproof camera (1) is used for observing the distribution condition of underwater corbicula fluminea before fishing so as to determine whether the distribution condition of the underwater corbicula fluminea meets the fishing condition or not; the fishing device is mainly composed of a fishing net cage (4), a pressing rod (5) and a dragging rope (6); the fishing net cage (4) is composed of a cage frame and a mesh body installed on the cage frame; the pressing rod (5) is fixed to the cage frame; the two ends of the dragging rope (6) are connected with the two sides of one face, not provided with the mesh body, of the cage frame; the lower bottom frame of one face, not provided with the mesh body, of the cage frame is additionally provided with spikes (8) inserted into river sludge conveniently. The complete equipment for investigation of the corbicula fluminea resources is simple and ingenious in structure, easy and convenient to operate, easy to implement and low in manufacturing cost.

The invention relates to a method for fast separating and extracting polypeptide and polysaccharide from corbicula fluminea. The method comprises the following steps: carrying out mashing pretreatment on corbicula fluminea meat; adding Alcalase alkaline protease of 1.5%-2.5% to a corbicula fluminea liquid with protein content of 1%-2%, regulating pH to 8-9, carrying out enzymolysis at 45-55 DEG C for 3 hours, carrying out enzyme deactivation at 90 DEG C for 10-15 minutes, and centrifugalizing to take supernate; separating the polypeptide through a macroporous adsorption resin column, loading a sample at the flow velocity of 1-2 BV/h and the concentration of 10-30 mg/ml, washing at the velocity of 2-4 BV/h, then resolving at the flow velocity of 1-3 ml/min by using ethanol, and carrying out rotary evaporation and concentration and freeze drying on a resolved solution to obtain corbicula fluminea polypeptide powder; adding a washing solution obtained from the last step to an ion-exchange chromatography column, respectively enabling an NaCl solution of 0.1-2 mol/L and deionized water to pass through the ion-exchange chromatography column at the flow velocity of 1-3 ml/min, collecting in steps, mixing same components, carrying out the rotary evaporation and concentration, dialyzing by using ionized water, carrying out the freeze drying on a dialyzed solution to obtain corbicula fluminea polysaccharide dry powder. The method disclosed by the invention is easy to operate and can be used for extracting the polypeptide and the polysaccharide at the same time and solving the problem that the polysaccharide is wasted when the polypeptide is extracted or the polypeptide is wasted when the polysaccharide is extracted. The polypeptide and the polysaccharide are high in yield and both have very good physicochemical property and functional property.

The invention discloses a breeding method for purifying lake and reservoir water quality by means of fishes, shrimps, clams and grass. According to the method, a lake and reservoir water quality purification ecological breeding region is established, and a three-dimensional breeding mode is formed by fully utilizing the fishes, shrimps, clams and grass at the upper layer, the middle layer, the lower layer and the bottom layer of the breeding region. The lake and reservoir water quality purification ecological breeding region is arranged at a lake and reservoir outlet, eutrophic lake water, obtained after aquaculture, of other regions of the lake and reservoir can flow to the lake and reservoir water quality purification ecological breeding region conveniently, and the lake and reservoir water quality purification ecological breeding region serves as the main feed source and plays the role of purifying the water quality. According to the method, only one feed is used, the use ratio of the feed is high, discharge of nitrogen, phosphorus and the like is low, and water environment protection is promoted. In addition, excreta of the fishes, shrimps and clams who take food is used for growth of hydrilla verticillata which provides natural fish nests for parents of yellow catfishes, freshwater shrimps and corbicula fluminea, and the natural fish nests are used for adhesion and incubation of zygotes of the fishes, shrimps and clams; meanwhile, hydrilla verticillata plays the role of improving the bottom environment of the breeding region, cyclic utilization of the fishes, shrimps, clams and grass in the lake and reservoir water quality purification ecological breeding region is formed, fishery development is achieved, and water environment protection is considered simultaneously.

The invention discloses a corbicula fluminea chili sauce and a making method thereof and belongs to the technical field of food processing. Fresh corbicula fluminea meat and stem-removed chilis respectively undergo steaming, cooking, enzyme deactivation and sterilization, then the fresh corbicula fluminea meat and the stem-removed chilis are respectively placed in an infrared drying box for dehydration and drying treatment, wherein the dried chilis also need to be chopped to make the chilis sauce. The dried corbicula fluminea meat and the red chili sauce are mixed with a proper amount of garlic, minced ginger paste, ground peanuts, sesame and spice seasonings, then mixing, stir-frying and baking operations are executed with edible vegetable oil, and finally packaging and cooling are executed to obtain the corbicula fluminea flavored chili sauce. The making method of the corbicula fluminea flavored chili sauce is simple and easy in process and convenient to operate, the deep processing way of corbicula fluminea meat is increased, and the variety of sauces is enriched. In addition, the obtained corbicula fluminea flavored chili sauce not only endows the chili sauce with the unique corbicula fluminea flavor, but also improves the nutritional value of the chili sauce, and has the advantages of being safe, nutritional and healthy.

The invention discloses a method for artificially culturing corbicula fluminea which has high survival rate and favorable stress resistance. The method comprises the following steps of: utilizing a corbicula fluminea individual with sexual maturity as a parent, artificially stimulating the corbicula fluminea individual until fertilized eggs are ovulated; collecting the fertilized eggs, placing the fertilized eggs into a hatching pond which has the water temperature of 20-22 DEG C, the pH of 7.3-7.8 and the salinity of 0, aerating and hatching the fertilized eggs, wherein the hatching density is 30-50 per ml; collecting D-shaped larvae, and culturing the D-shaped larvae, wherein the culturing density is 2-3 per ml, the culturing water is the filtered river water, the temperature is 20-22 DEG C, the pH is 7.3-7.8, the salinity is 0, the water is changed once or twice per day, and the water-changing amount is 1/3-1/2; feeding fresh water chlamydomonas baits with the density of 5-8 cell*103 per ml; when the D-shaped larvae grow as long as 190-210 mums, increasing the feeding amount to 3-5*104cell per ml, and culturing the D-shaped larvae to metamorphosis larvae; and culturing the metamorphosis larvae with the feeding amount of 5-8*104cell per ml until young conches grow to 1-2mm.

The invention discloses low-cost nutrition-balanced giant salamander aquatic feed. The low-cost nutrition-balanced giant salamander aquatic feed comprises the following raw materials in parts by weight: 50-60 parts of earthworms, 30-40 parts of silkworm chrysalis, 20-25 parts of water earthworms, 5-10 parts of wigglers, 10-15 parts of diplopods, 14-18 parts of pelteobagrus fulvidraco, 5-10 parts of parabramis pekinensis, 15-20 parts of snakeheads, 20-25 parts of silver carps, 20-25 parts of bighead carps, 5-10 parts of basses, 5-10 parts of mantis shrimps, 10-20 parts of black tiger shrimps, 2-5 parts of small-sized shrimps, 10-20 parts of ruditapes philippinarum, 15-20 parts of field snails, 10-20 parts of corbicula fluminea, 10-20 parts of extruded soybeans, 10-20 parts of fermented peanut meal, 30-40 parts of corn protein powder, 20-30 parts of skim milk powder, 10-15 parts of egg white, 5-10 parts of malic acid, 3-6 parts of locust bean gum, 5-8 parts of gelatin, 2-5 parts of grape seed oil, 1-3 parts of composite vitamin, 1.5-2 parts of composite minerals, and 1-2 parts of a probiotic preparation. According to the low-cost nutrition-balanced giant salamander aquatic feed, the cost is reduced, and the nutrition is comprehensive, rich and balanced.

The invention discloses a method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra. The method includes that: the medicinal whitmania pigra is used as an object; a mixing culture and killing experiment of the pomacea canaliculata and the medicinal whitmania pigra, a weight gain experiment of the medicinal whitmania pigra, and a difference contrast experiment of predation of the medicinal whitmania pigra on male and female individuals of the pomacea canaliculata are performed based on life habits that the medicinal whitmania pigra feeds on sap sucked from mollusks such as viviparid, clam, and corbicula fluminea;the difference contrast experiment of predation of the medicinal whitmania pigra on the male and female individuals of the pomacea canaliculata shows that: when the predation number difference of the medicinal whitmania pigra fluctuates between 0 and 0.4, the predation of the medicinal whitmania pigra on the pomacea canaliculata has no gender difference; when the number and the density of the pomacea canaliculata are large, the number ratio of the the pomacea canaliculata to the medicinal whitmania pigra is more than 3 : 1, the pomacea canaliculata eats dead bodies of the medicinal whitmania pigra, but in this environment, the accumulation predation number of the medicinal whitmania pigra on the pomacea canaliculata reaches maximum, and it verifies that the certain control effect of the medicinal whitmania pigra on the pomacea canaliculata can be achieved.

The invention relates to a high-yielding and propagating Corbicula fluminea culture method. The method is characterized by comprising the following steps: firstly, selecting a propagating culture area, which is required to be far away from shipping thoroughfares and estuaries for water intake and drainage and cannot be a settled fish spawning site in a lake; secondly, carrying out purse seine in the selected propagating culture area, and controlling the area of a purse seine to 3hm<2> to 5hm<2>, wherein the purse seine should meet the requirements on preventing cultured fishes from escaping and preventing natural enemy fishes of Corbicula fluminea outside the purse seine from entering, and the height of the purse seine should be higher than the highest water level of the culture area over the years by at least 50cm; thirdly, selecting waveless weather, and carrying out fish eradication by adopting a ground cage and netting method; fourthly, carrying out young Corbicula fluminea stocking: selecting Corbicula fluminea, of which the shell length is 0.5cm to 1.2cm, the individual weight is 0.5g to 1g and the number per kilogram is 1,500 to 2,200, as offspring, and carrying out soaking for 10 to 20 minutes in a 2% to 4% common-salt aqueous solution before stocking for disinfecting; fifthly, stocking propagating fishes: selecting Hypophthalmichthys molitrix and Aristichthys nobilis as propagating culture fishes; and sixthly, strengthening daily management. The method disclosed by the invention is simple and is obvious in economic efficiency.

The invention discloses a method of removing fishy odor and bitter taste and increasing fragrance for enzymatic hydrolysate of corbicula fluminea flesh. The method includes the steps of: 1) preparation of the enzymatic hydrolysate: enzymolyzing corbicula fluminea flesh pulp in certain material-to-liquid ratio with an enzyme mixture, and deactivating the enzyme to obtain the enzymatic hydrolysate;2) fishy odor and bitter taste removal: regulating pH value of the enzymatic hydrolysate and performing activated carbon adsorption at certain temperature, and performing centrifugation to prepare fishy odor and bitter taste removed enzymatic hydrolysate; 3) fragrance increase: performing Maillard reaction with amino acids or proteins and reducing sugar to treat the fishy odor and bitter taste removed enzymatic hydrolysate. The produced liquid has a mellow flavor of the corbicula fluminea and has a pleasant color. In the invention, through the activated carbon powder adsorption, the corbiculafluminea enzymatic hydrolysate is subjected to the fishy odor and bitter taste removal, wherein adsorption conditions are optimized; then through the Maillard reaction, the corbicula fluminea enzymatic hydrolysate is subjected to the fragrance increase treatment. The prepared reaction liquid has a slight brown color, has a mellow fragrance and abundant nutrients. The product can be used as a foodseasoning. The process supplies basis to improvement of quality and additional value of highly processed products of the corbicula fluminea and lays the fundament of development of new market of seasonings.

The present invention discloses a microwave-assisted extraction and enzymatic preparation method of a sober-up Corbicula fluminea juice. The method comprises the following steps: temporarily cultivating Corbicula fluminea, carrying out sand removal, washing, adding water, and carrying out microwave-assisted extraction; carrying out vacuum suction filtration, adjusting pH value of the filtrate, adding proteases, and carrying out microwave-assisted digestion; and performing enzyme inactivation treatment, and separating and concentrating with an ultrafiltering membrane to obtain the sober-up Corbicula fluminea juice. Compared with the existing hot-water extraction method, active ingredients of the Corbicula fluminea are fully extracted by the microwave in the present invention; proteolysis and ultrafiltration separation concentration technology are combined, such that the extraction time is short, the active ingredients content in the Corbicula fluminea is high and sober-up effect is remarkable. The process is simple, efficient, time-saving and yield-increasing. The prepared Corbicula fluminea juice has a significant sober-up effect, and is suitable for industrial production.

The invention discloses a method for separating and quantitatively analyzing food-borne nanoparticles. The method comprises the following steps of: (1) performing Corbicula fluminea hot processing to obtain Corbicula fluminea soup, centrifuging the Corbicula fluminea soup to obtain a supernatant, and performing freeze-drying treatment of the supernatant to obtain freeze-dried powder; (2) separating the functional nano particles in the freeze-dried powder solution by using capillary electrophoresis; and (3) calculating the concentration or number per volume of functional nanoparticles obtained in the step (2) by a linear regression equation. The method has the advantages of rapid separation, the good separation effect, the high sensitivity, the good reproducibility, the low detection limit, the small sample injection amount, capability of performing quantitative analysis on the functional nanoparticles and the like.

The invention discloses a nutritious corbicula fluminea juice and a preparation method thereof and relates to the technical field of preparation methods of corbicula fluminea juices. Shelled corbicula fluminea meat is mixed with water and subjected to homogenizing treatment to obtain corbicula fluminea meat paste, the corbicula fluminea meat paste is subjected to high-pressure pulsed electric field-ultrasonic composite treatment to obtain an initial corbicula fluminea juice, and then a dynamic complex protease is adopted for carrying out enzymolysis operation to obtain a concentrated corbicula fluminea juice; the concentrated corbicula fluminea juice is subjected to centrifugation and sterilization operation under a non-thermal pulse electric field to obtain the nutritious corbicula fluminea juice. Not only can the processing added value of corbicula fluminea be increased, the product type of the corbicula fluminea is enriched, but also industrial upgrading can be promoted, and the market competitiveness of products is improved. In addition, the nutritious corbicula fluminea juice obtained by means of the method not only is rich in nutrients such as amino acid, polypeptide and polysaccharide, clear and bright in appearance and unique in flavor but also has high in-vitro anti-oxidation activity.

The present invention discloses a corbicula fluminea health-care soft candy and a preparation method thereof. The corbicula fluminea health-care soft candy is mainly prepared from the following raw materials in parts by weight: 10-14 parts of corbicula fluminea juice, 0.7-1.3 parts of dried tangerine peel powder, 35-45 parts of glucose syrup, 30-45 parts of xylitol, 5-10 parts of gelatin, 0.9-1.5parts of high-fat pectin, 0.5-0.9 part of loquat powder and 0.1-0.5 part of citric acid. The prepared corbicula fluminea health-care soft candy is pleasant in mouthfeel, moderate in softness and hardness and good in chewability, and the corbicula fluminea deep processing product easily accepted by the public, also has functions of preventing intoxitation, dispelling alcoholism, protecting liver, preventing oxidation, etc., is rich in nutrients, contains rich carotene and various multivitamins, is easy to carry and meets consumer demands. The corbicula fluminea health-care soft candy is simpleand convenient in the preparation method and wide in raw material sources, and can be applied to industrial large-scale production applications.

The invention provides an enrichment and extraction method of mitochondrial whole genome DNA of corbicula fluminea, and a method of identifying the corbicula fluminea through mitochondrial genes. Through the enrichment and extraction method provided by the invention, the sufficient mitochondrial genome DNA of the corbicula fluminea can be successfully obtained. A primer pair Mit1 of an nad2-cox3 gene is designed according to the mitochondrial sequence of the corbicula fluminea, and a primer pair Mit2 is designed according to a cob gene segment; the two primer pairs provided by the invention are high in specificity, the mitochondrial sequence of the corbicula fluminea can be specifically amplified, and for mitochondria of other species, the gene segment cannot be formed by amplification; and the advantages of good amplification performance and discrimination, few by-products, and the like are achieved, and the primer pairs can be used for species identification of the corbicula fluminea.