17 results about "Hypophthalmichthys" patented technology

The application discloses a kind of silver carp variety specific SNP molecular marker combination, silver carp DNA fingerprint and application, the SNP molecular marker combination includes 367 SNP sites, the site information of the SNP molecular marker combination is as shown in table 1.The application takes silver carp germplasm resource as research object, obtains genotype by whole genome resequencing method, and then filters out 367 specific SNP markers capable of distinguishing different silver carp varieties, and the DNA fingerprint based on these markers can be used to distinguish different silver carp varieties.Compared with traditional methods, the fingerprint has the characteristics of high accuracy and strong reliability when used for silver carp variety identification.This technology not only provides strong support for the specific evaluation of silver carp varieties, the protection and management of breed rights, and the optimization and structure adjustment of silver carp breeding industry, but also shows good application prospects in the classification of silver carp germplasm resources, the confirmation of breed rights and the tracing of individual sources.

The present application relates to the field of biotechnology, and more particularly to a mylopharyngodon piceus gender-specific molecular marker, primer and application thereof, wherein the primer designed for the molecular marker is used for PCR amplification, and the genetic sex of the mylopharyngodon piceus can be accurately and rapidly identified. The molecular marker presents stable difference between male and female individuals, and the identification accuracy is 100%. In addition, the molecular marker can be stably applied to mylopharyngodon piceus of different specifications and different breeding strains, and shows good universality and reliability. The present application provides important technical support for the unisexual breeding, germplasm resource management and population structure monitoring of mylopharyngodon piceus, and has wide application prospect.

The utility model discloses a kind of unloading's hypophthalmichthys nobilis square block hooks, including buoyancy plate and main line, the outer surface of the buoyancy plate is fixedly connected with a group of extension rods, the end of each extension rod away from buoyancy plate is fixedly connected with middle pipe arm, the inside of the buoyancy plate is fixedly embedded with bearing, the outer surface of the buoyancy plate is equipped with cover bait plate.The device is cooperated with first spring and second spring by the sub-line hook and intermediate line hook of setting, when hypophthalmichthys nobilis fish hook struggles violently, the great impact force generated by it can be converted into spring's elastic potential energy, thereby being effectively buffered and absorbed, avoid instantaneous rigid tensile force exceeding fish hook strength or fish lip tissue bearing limit, leading to fishing hook failure, can effectively solve the problem that because hypophthalmichthys nobilis is middle upper layer filter-feeding fish, impact force is stronger, many traditional hypophthalmichthys nobilis square block hooks cannot carry out unloading when fishing group is impacted, prone to unhooking phenomenon, will seriously affect user fishing experience problem.

The application belongs to the technical field of fish breeding, and discloses a method for knocking out PHD3 gene of Hypophthalmichthys molitrix and application thereof in creating new germplasm of low-oxygen-tolerant Hypophthalmichthys molitrix. Taking PHD3 gene as a target gene, two gRNAs targeting the first exon of PHD3 gene are synthesized by in vitro transcription, mixed with Cas9 protein, and used to edit genes of wild-type Hypophthalmichthys molitrix, so as to improve the knocking-out efficiency of PHD3 gene, and then F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are efficiently obtained. The F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are subjected to low-oxygen stress experiment, and the result shows that the low-oxygen tolerance of the PHD3 knockout mutants of Hypophthalmichthys molitrix is significantly improved compared with the wild type. The application successfully realizes efficient knocking out of PHD3 gene of Hypophthalmichthys molitrix and obtains Hypophthalmichthys molitrix PHD3 mutants with significantly enhanced low-oxygen tolerance, thereby providing an effective technical means for carrying out functional research and genetic improvement of Hypophthalmichthys molitrix, and having important industrial application value.

The invention provides a protein glutaminase mutant with synchronously improved enzyme activity and thermal stability and application of the protein glutaminase mutant, and belongs to the field of gene engineering and enzyme engineering. According to the invention, a series of mutants MT1-MT6 are obtained by carrying out molecular modification on a coding gene of protein glutaminase PG3 from Chryseobacterium sp. Compared with a wild type, the mutant MT6 has the advantages that the optimal catalytic temperature of the mutant MT6 is increased by 5 DEG C, the specific activity and the specific activity t65 DEG C 1 / 2 are 106.1 U / mg and 76.7 min respectively and are 2.69 times and 2.99 times of the specific activity t65 DEG C 1 / 2 of the wild type, the purpose of simultaneously improving the catalytic activity and the thermal stability of the protein glutaminase PG3 is achieved, and the application potential of the protein glutaminase in the industrial field is expanded. In addition, the mutant can significantly improve the foamability and stability of the myofibrillar protein of the spotted silver carp, and provides a potential enzyme resource for enhancing the functional characteristics of the protein through enzymatic deamidation modification.

The invention provides a protein glutaminase mutant as well as a coding gene and application thereof, and belongs to the field of gene engineering and enzyme engineering. According to the invention, a series of mutants MT1-MT8 are obtained by carrying out molecular modification on a coding gene of chitophage protein glutaminase PG5. Compared with a wild type, the mutant has the advantages that the optimal catalytic temperature is increased by 5 DEG C, the specific activity and t55 DEG C 1 / 2 are 31.1 U / mg and 216.2 min respectively and are 2.99 times and 3.57 times that of the wild type, and the application potential of protein glutaminase in the industrial field is expanded. Besides, the mutant can significantly improve dissolution of the myofibrillar protein of the spotted silver carp under the condition of a salt-free system, provides a potential enzyme resource for enzymatic deamidation modification and enhancement of protein functional characteristics, and has a wide market application prospect.

The invention belongs to the technical field of fish breeding, and discloses a silver carp PHD3 gene knockout method and application of the silver carp PHD3 gene knockout method in creation of new hypoxia-resistant silver carp germplasm. A PHD3 gene is taken as a target gene, gRNA of the first exon of two targeted PHD3 genes is synthesized through in-vitro transcription, the gRNA is mixed with Cas9 protein, gene editing is carried out on wild silver carp, the PHD3 gene knockout efficiency is improved, and then the silver carp PHD3 gene knockout F0-generation mutant is efficiently obtained. The hypoxia stress experiment is carried out on the PHD3 gene knockout F0-generation mutant silver carp, and the result shows that the hypoxia resistance of the PHD3 knockout mutant of the silver carp is obviously improved compared with that of a wild type. Efficient knockout of the silver carp PHD3 gene is successfully achieved, the silver carp PHD3 mutant with remarkably enhanced hypoxia tolerance is obtained, an effective technical means is provided for functional research and genetic improvement of hypoxia tolerance and the like of silver carps, and the silver carp PHD3 mutant has important industrial application value.

The invention relates to chitophage protein glutaminase PG5, a coding gene and application of the chitophage protein glutaminase PG5, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG5 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG5 is as shown in SEQ ID No. 2. A large amount of chitophage-derived protein glutaminase PG5 is prepared through an escherichia coli expression system, and the protein glutaminase PG5 is used for carrying out enzymatic deamidation modification on the myofibrillar protein of the spotted silver carp, so that the thermal reversible protein gel is successfully prepared in a salt-free system. An important tool enzyme is provided for protein deamidation modification, unprecedented flexibility is provided for processing adaptability and cold-chain storage and transportation of fish protein gel products, and the application range of the fish protein gel products in food design is greatly expanded.

The application belongs to the method for improving fish quality. A method for improving the quality of fresh fish by using a composite curing agent, characterized in that it comprises the following steps: 1) raw material treatment: take fresh hypophthalmichthys nobilis, remove scales, remove internal organs, wash and cut into uniform small pieces to obtain hypophthalmichthys nobilis meat; 2) preparation of protease solution; 3) extraction of tea leaf extract; 4) preparation of composite curing agent: according to 100g of hypophthalmichthys nobilis meat, 1.5g of tea leaf extract, 0.5g of protease solution and 2.0g of salt, select the tea leaf extract, the protease solution and the salt, and mix to obtain the composite curing agent; 5) curing of fresh fish: according to the proportion described in step 4), take the hypophthalmichthys nobilis meat, add the composite curing agent, and mix thoroughly until slime appears on the surface of the fish meat; use a container to fill, wrap the container opening with preservative film, seal and place in a 4℃ refrigerator for cold storage for 4d. The method has a good synergistic deodorization and quality improvement effect on fresh fish.

The application relates to the technical field of judging or confirming hypophthalmichthys nobilis, and particularly relates to amino acid biomarkers for distinguishing wild hypophthalmichthys nobilis from cultured hypophthalmichthys nobilis, methods and purposes. The biomarkers comprise at least one of aspartic acid, alanine, phenylalanine, histidine and arginine. The biomarkers can accurately distinguish wild hypophthalmichthys nobilis from cultured hypophthalmichthys nobilis, provide scientific basis and technical support for relevant law enforcement departments, strengthen supervision and strike intensity of illegal fishing behavior in the Yangtze River basin, reduce overfishing of wild resources, and further provide a technical basis for better management and protection of hypophthalmichthys nobilis resources.

The application discloses a method for culturing and screening of zygotes of Megalobrama amblycephala, and steps include: S1, culturing parent fish in a light-proof incubator; S2, a conveying belt is used for receiving and conveying the discharged zygotes; S3, automatic screening is performed according to the characteristics of live eggs and dead eggs, and a collecting assembly is used for cleaning and collecting the live eggs with sticky characteristics; S4, a piston plate reciprocates in a piston cylinder, gas is discharged to the surface of the conveying belt through a bubble tube, and secondary screening is performed on the zygotes; and S5, a piston rod moves to drive a regulating assembly to work through a friction plate, so that the movement speed of the collecting assembly is adjusted. Through the automatic screening of the characteristics of the live eggs and the dead eggs in the conveying process, and the secondary screening of the gas discharged to the surface of the conveying belt through the bubble tube when the conveying assembly works, the piston rod drives the piston plate to reciprocate in the piston cylinder, the dead eggs can be effectively removed, and the overall fertilization rate of the zygotes is improved.

The invention discloses a composition, a kit and a method for detecting Huso dauricus mitochondrial genes. A specific primer probe composition is designed aiming at the Huso dauricus mitochondrial ND4 gene, and the corresponding nucleotide sequences of the specific primer probe composition are SEQ ID No.1-3. By taking the Huso dauricus mitochondrial ND4 gene as a core technical support, the invention further constructs and optimizes a real-time fluorescent PCR specific detection scheme based on the Huso dauricus mitochondrial ND4 gene. According to the scheme, a part of sequence of a Huso dauricus mitochondrial ND4 gene is selected as a target fragment, a primer and a TaqMan-MGB probe are directionally designed, and finally a set of detection system with rapidness, high sensitivity and strong specificity is successfully established. The detection system can be effectively applied to adulteration identification and germplasm resource identification of huso dauricus products, and has very wide industrial popularization and application prospects.

The invention relates to the technical field of biology, in particular to a silver carp sex specific molecular marker and a primer and application thereof, and the primer designed for the molecular marker is used for PCR amplification, so that the hereditary sex of silver carp can be accurately and rapidly identified. The molecular marker shows stable difference between male and female individuals, and the identification accuracy rate reaches 100%. In addition, the molecular marker can be stably applied to silver carps of different specifications and different culture lines, and shows good universality and reliability. The method provides important technical support for unisexual breeding, germplasm resource management and population structure monitoring of silver carps, and has a wide application prospect.

The invention discloses a silver carp variety specific SNP (Single Nucleotide Polymorphism) molecular marker combination, a silver carp DNA (Deoxyribose Nucleic Acid) fingerprint spectrum and application, the SNP molecular marker combination comprises 367 SNP sites, and the site information of the SNP molecular marker combination is shown in a table 1. Silver carp germplasm resources are used as research objects, genotypes are obtained through a whole genome re-sequencing method, then 367 specific SNP markers capable of distinguishing different silver carp varieties are screened out, and the DNA fingerprint spectrum constructed based on the markers can be used for distinguishing the different silver carp varieties. Compared with a traditional method, the fingerprint spectrum has the characteristics of high accuracy and high reliability when being used for identifying the varieties of the silver carps, and the technology provides powerful support for specific evaluation of the varieties of the silver carps, protection and management of the variety right and variety optimization and structure adjustment of the breeding industry of the silver carps; good application prospects are shown in the aspects of silver carp germplasm resource classification, variety right confirmation, individual source tracing and the like.

The present application relates to the technical field of aquaculture, in particular to a preparation method and application of a fertilizer for reservoir culture of Hypophthalmichthys nobilis. The fertilizer comprises corn starch residue, distiller's grains, sugar cane residue, cerium oxide, lanthanum oxide, Chinese herbal medicine extract, brewing yeast, animal bifidobacterium, clostridium butyricum, acid protease, ferrous sulfate, magnesium sulfate, zinc sulfate, copper sulfate, citric acid, sodium alginate, calcium chloride, silicon dioxide and calcium carbonate. The present application realizes efficient utilization of rare earth, fermentation product enrichment and nutrient slow release by pre-complexing rare earth with citric acid, stepwise fermentation combined with pH regulation and calcium alginate microcapsule granulation, and effectively promotes rapid growth of Hypophthalmichthys nobilis.

The application relates to a winter caught hypophthalmichthys nobilis classification and recognition method based on a DAG-SwishNet network, and belongs to the technical field of image recognition and deep learning. An improved network architecture is proposed, the ShuffleNet backbone is optimized, an inflation convolution pyramid module is introduced, multi-inflation rate parallel convolution is used to capture multi-scale features, and the recognition ability for details such as the body size and scales of the hypophthalmichthys nobilis is enhanced; a global grouping double-pooling coordinate attention mechanism is designed, spatial attention weights are generated through grouping double-pooling and shared convolution layers, target regions are highlighted, and background interference is suppressed; a self-adaptive Swish-ReLU fusion function is used to replace a traditional ReLU, the learnable parameter alpha is used to dynamically balance the Swish smoothness and the ReLU sparsity, and the complex feature modeling capability is improved; in the training, an ElasticNet regularization and a cosine annealing learning rate strategy are combined, and the model generalization and convergence stability are enhanced.

The application relates to the technical field of judging or confirming hypophthalmichthys nobilis, and particularly relates to a fatty acid biomarker for distinguishing wild and cultured hypophthalmichthys nobilis, a method and an application. The biomarker comprises at least one of tetradecanoic acid, cis, cis, cis-9, 12, 15-octadecatrienoic acid, cis-5, 8, 11, 14-eicosatetraenoic acid and cis-5, 8, 11, 14, 17-eicosapentaenoic acid. The biomarker can accurately distinguish wild and cultured hypophthalmichthys nobilis, provide scientific basis and technical support for relevant law enforcement departments, strengthen supervision and strike force of illegal fishing behavior in the Yangtze River basin, reduce overfishing of wild resources, and further provide a technical basis for better management and protection of hypophthalmichthys nobilis resources.