8 results about "Hypophthalmichthys" patented technology

The application discloses a kind of silver carp variety specific SNP molecular marker combination, silver carp DNA fingerprint and application, the SNP molecular marker combination includes 367 SNP sites, the site information of the SNP molecular marker combination is as shown in table 1.The application takes silver carp germplasm resource as research object, obtains genotype by whole genome resequencing method, and then filters out 367 specific SNP markers capable of distinguishing different silver carp varieties, and the DNA fingerprint based on these markers can be used to distinguish different silver carp varieties.Compared with traditional methods, the fingerprint has the characteristics of high accuracy and strong reliability when used for silver carp variety identification.This technology not only provides strong support for the specific evaluation of silver carp varieties, the protection and management of breed rights, and the optimization and structure adjustment of silver carp breeding industry, but also shows good application prospects in the classification of silver carp germplasm resources, the confirmation of breed rights and the tracing of individual sources.

The present application relates to the field of biotechnology, and more particularly to a mylopharyngodon piceus gender-specific molecular marker, primer and application thereof, wherein the primer designed for the molecular marker is used for PCR amplification, and the genetic sex of the mylopharyngodon piceus can be accurately and rapidly identified. The molecular marker presents stable difference between male and female individuals, and the identification accuracy is 100%. In addition, the molecular marker can be stably applied to mylopharyngodon piceus of different specifications and different breeding strains, and shows good universality and reliability. The present application provides important technical support for the unisexual breeding, germplasm resource management and population structure monitoring of mylopharyngodon piceus, and has wide application prospect.

The application belongs to the technical field of fish breeding, and discloses a method for knocking out PHD3 gene of Hypophthalmichthys molitrix and application thereof in creating new germplasm of low-oxygen-tolerant Hypophthalmichthys molitrix. Taking PHD3 gene as a target gene, two gRNAs targeting the first exon of PHD3 gene are synthesized by in vitro transcription, mixed with Cas9 protein, and used to edit genes of wild-type Hypophthalmichthys molitrix, so as to improve the knocking-out efficiency of PHD3 gene, and then F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are efficiently obtained. The F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are subjected to low-oxygen stress experiment, and the result shows that the low-oxygen tolerance of the PHD3 knockout mutants of Hypophthalmichthys molitrix is significantly improved compared with the wild type. The application successfully realizes efficient knocking out of PHD3 gene of Hypophthalmichthys molitrix and obtains Hypophthalmichthys molitrix PHD3 mutants with significantly enhanced low-oxygen tolerance, thereby providing an effective technical means for carrying out functional research and genetic improvement of Hypophthalmichthys molitrix, and having important industrial application value.

The invention belongs to the technical field of fish breeding, and discloses a silver carp PHD3 gene knockout method and application of the silver carp PHD3 gene knockout method in creation of new hypoxia-resistant silver carp germplasm. A PHD3 gene is taken as a target gene, gRNA of the first exon of two targeted PHD3 genes is synthesized through in-vitro transcription, the gRNA is mixed with Cas9 protein, gene editing is carried out on wild silver carp, the PHD3 gene knockout efficiency is improved, and then the silver carp PHD3 gene knockout F0-generation mutant is efficiently obtained. The hypoxia stress experiment is carried out on the PHD3 gene knockout F0-generation mutant silver carp, and the result shows that the hypoxia resistance of the PHD3 knockout mutant of the silver carp is obviously improved compared with that of a wild type. Efficient knockout of the silver carp PHD3 gene is successfully achieved, the silver carp PHD3 mutant with remarkably enhanced hypoxia tolerance is obtained, an effective technical means is provided for functional research and genetic improvement of hypoxia tolerance and the like of silver carps, and the silver carp PHD3 mutant has important industrial application value.

The application belongs to the method for improving fish quality. A method for improving the quality of fresh fish by using a composite curing agent, characterized in that it comprises the following steps: 1) raw material treatment: take fresh hypophthalmichthys nobilis, remove scales, remove internal organs, wash and cut into uniform small pieces to obtain hypophthalmichthys nobilis meat; 2) preparation of protease solution; 3) extraction of tea leaf extract; 4) preparation of composite curing agent: according to 100g of hypophthalmichthys nobilis meat, 1.5g of tea leaf extract, 0.5g of protease solution and 2.0g of salt, select the tea leaf extract, the protease solution and the salt, and mix to obtain the composite curing agent; 5) curing of fresh fish: according to the proportion described in step 4), take the hypophthalmichthys nobilis meat, add the composite curing agent, and mix thoroughly until slime appears on the surface of the fish meat; use a container to fill, wrap the container opening with preservative film, seal and place in a 4℃ refrigerator for cold storage for 4d. The method has a good synergistic deodorization and quality improvement effect on fresh fish.

The application relates to the technical field of judging or confirming hypophthalmichthys nobilis, and particularly relates to amino acid biomarkers for distinguishing wild hypophthalmichthys nobilis from cultured hypophthalmichthys nobilis, methods and purposes. The biomarkers comprise at least one of aspartic acid, alanine, phenylalanine, histidine and arginine. The biomarkers can accurately distinguish wild hypophthalmichthys nobilis from cultured hypophthalmichthys nobilis, provide scientific basis and technical support for relevant law enforcement departments, strengthen supervision and strike intensity of illegal fishing behavior in the Yangtze River basin, reduce overfishing of wild resources, and further provide a technical basis for better management and protection of hypophthalmichthys nobilis resources.

The invention discloses a composition, a kit and a method for detecting Huso dauricus mitochondrial genes. A specific primer probe composition is designed aiming at the Huso dauricus mitochondrial ND4 gene, and the corresponding nucleotide sequences of the specific primer probe composition are SEQ ID No.1-3. By taking the Huso dauricus mitochondrial ND4 gene as a core technical support, the invention further constructs and optimizes a real-time fluorescent PCR specific detection scheme based on the Huso dauricus mitochondrial ND4 gene. According to the scheme, a part of sequence of a Huso dauricus mitochondrial ND4 gene is selected as a target fragment, a primer and a TaqMan-MGB probe are directionally designed, and finally a set of detection system with rapidness, high sensitivity and strong specificity is successfully established. The detection system can be effectively applied to adulteration identification and germplasm resource identification of huso dauricus products, and has very wide industrial popularization and application prospects.

The present application relates to the technical field of aquaculture, in particular to a preparation method and application of a fertilizer for reservoir culture of Hypophthalmichthys nobilis. The fertilizer comprises corn starch residue, distiller's grains, sugar cane residue, cerium oxide, lanthanum oxide, Chinese herbal medicine extract, brewing yeast, animal bifidobacterium, clostridium butyricum, acid protease, ferrous sulfate, magnesium sulfate, zinc sulfate, copper sulfate, citric acid, sodium alginate, calcium chloride, silicon dioxide and calcium carbonate. The present application realizes efficient utilization of rare earth, fermentation product enrichment and nutrient slow release by pre-complexing rare earth with citric acid, stepwise fermentation combined with pH regulation and calcium alginate microcapsule granulation, and effectively promotes rapid growth of Hypophthalmichthys nobilis.