3 results about "Hypophthalmichthys" patented technology

The application belongs to the technical field of fish breeding, and discloses a method for knocking out PHD3 gene of Hypophthalmichthys molitrix and application thereof in creating new germplasm of low-oxygen-tolerant Hypophthalmichthys molitrix. Taking PHD3 gene as a target gene, two gRNAs targeting the first exon of PHD3 gene are synthesized by in vitro transcription, mixed with Cas9 protein, and used to edit genes of wild-type Hypophthalmichthys molitrix, so as to improve the knocking-out efficiency of PHD3 gene, and then F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are efficiently obtained. The F0 generation mutants of Hypophthalmichthys molitrix with PHD3 gene knocked out are subjected to low-oxygen stress experiment, and the result shows that the low-oxygen tolerance of the PHD3 knockout mutants of Hypophthalmichthys molitrix is significantly improved compared with the wild type. The application successfully realizes efficient knocking out of PHD3 gene of Hypophthalmichthys molitrix and obtains Hypophthalmichthys molitrix PHD3 mutants with significantly enhanced low-oxygen tolerance, thereby providing an effective technical means for carrying out functional research and genetic improvement of Hypophthalmichthys molitrix, and having important industrial application value.

The application belongs to the method for improving fish quality. A method for improving the quality of fresh fish by using a composite curing agent, characterized in that it comprises the following steps: 1) raw material treatment: take fresh hypophthalmichthys nobilis, remove scales, remove internal organs, wash and cut into uniform small pieces to obtain hypophthalmichthys nobilis meat; 2) preparation of protease solution; 3) extraction of tea leaf extract; 4) preparation of composite curing agent: according to 100g of hypophthalmichthys nobilis meat, 1.5g of tea leaf extract, 0.5g of protease solution and 2.0g of salt, select the tea leaf extract, the protease solution and the salt, and mix to obtain the composite curing agent; 5) curing of fresh fish: according to the proportion described in step 4), take the hypophthalmichthys nobilis meat, add the composite curing agent, and mix thoroughly until slime appears on the surface of the fish meat; use a container to fill, wrap the container opening with preservative film, seal and place in a 4℃ refrigerator for cold storage for 4d. The method has a good synergistic deodorization and quality improvement effect on fresh fish.

The present application relates to the technical field of aquaculture, in particular to a preparation method and application of a fertilizer for reservoir culture of Hypophthalmichthys nobilis. The fertilizer comprises corn starch residue, distiller's grains, sugar cane residue, cerium oxide, lanthanum oxide, Chinese herbal medicine extract, brewing yeast, animal bifidobacterium, clostridium butyricum, acid protease, ferrous sulfate, magnesium sulfate, zinc sulfate, copper sulfate, citric acid, sodium alginate, calcium chloride, silicon dioxide and calcium carbonate. The present application realizes efficient utilization of rare earth, fermentation product enrichment and nutrient slow release by pre-complexing rare earth with citric acid, stepwise fermentation combined with pH regulation and calcium alginate microcapsule granulation, and effectively promotes rapid growth of Hypophthalmichthys nobilis.