Method for separating and quantitatively analyzing food-borne nanoparticles

A technology for quantitative analysis of nanoparticles, applied in particle and sedimentation analysis, particle size analysis, analysis of materials, etc., can solve the problems of inability to quantitatively analyze and elute food-borne nanoparticles, achieve low detection limit, high sensitivity, Effect of small injection volume
CN110286098APending Publication Date: 2019-09-27ZHEJIANG GONGSHANG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG GONGSHANG UNIVERSITY
Publication Date
2019-09-27

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Abstract

The invention discloses a method for separating and quantitatively analyzing food-borne nanoparticles. The method comprises the following steps of: (1) performing Corbicula fluminea hot processing to obtain Corbicula fluminea soup, centrifuging the Corbicula fluminea soup to obtain a supernatant, and performing freeze-drying treatment of the supernatant to obtain freeze-dried powder; (2) separating the functional nano particles in the freeze-dried powder solution by using capillary electrophoresis; and (3) calculating the concentration or number per volume of functional nanoparticles obtained in the step (2) by a linear regression equation. The method has the advantages of rapid separation, the good separation effect, the high sensitivity, the good reproducibility, the low detection limit, the small sample injection amount, capability of performing quantitative analysis on the functional nanoparticles and the like.
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Description

technical field

[0001] The invention belongs to the field of food and nanometer material analysis, in particular to a method for separation and quantitative analysis of foodborne nanometer particles. Background technique

[0002] Capillary Electrophoresis (Capillary Electrophoresis, CE), also known as High Performance Capillary Electrophoresis (HPCE), the separation site is a capillary (inner diameter 10-200nm), the driving force is a high-voltage electric field, and the charged particles are different according to their mobility and distribution coefficient Electrophoresis technology for rapid separation. Based on the following advantages, capillary electrophoresis has been widely used in separation and analysis: (1) high separation efficiency, column efficiency can reach 10 7 / m or more; (2) fast speed, the detection time is usually more than ten seconds to ten minutes; (3) wide range of analysis, from small molecular substances to macromolecular polymers, used in life sc...

Claims

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