57results about How to "Rapid separation and purification" patented technology

The invention relates to a multifunctional double-layer core-shell structure magnetic nano particle. In the invention, a magnetic nano particle with a particle size of 1-300 nm is used as a core and coated with a double-layer shell consisting of a SiO2 layer with a thickness of 1-200 nm and a hydrolyzed silane coupling agent layer is 1-100 nm thick and comprises one or more multifunctional groups; the particle size and the shell layer thickness can be controlled through regulating the volumes, the weight ratios and the reaction time of the magnetic core, a silicon dioxide precursor, a silane coupling agent and a catalyst in a preparation process; the total particle size of the nano particle can be as small as 5-50 nm and as large as 700-800 nm; the nano particle can have superparamagnetism, paramagnetism and ferromagnetism according to the change of the magnetic core particle size; and one or more bioactive molecules can be connected into the shell layer of the magnetic nano particle or to the surface of the shell layer through a chemical method or a physical method. The invention also provides a preparation method of the multifunctional double-layer core-shell structure magnetic nano particle and application thereof. The particle preparation method has the advantages of simplicity, moderate condition, low cost and easy realization of industrial production. The nano particle can obtain different functions through connecting different bioactive molecules and can be applied to the fields of protein enrichment, biological detection, separation and purification, targeted drug carriers, cell imaging and medical imaging.

The invention discloses a full-automatic QuECHERS experimental device and an experimental method. The full-automatic QuECHERS experimental device comprises a multifunctional mechanical arm, a cap screwing module, a liquid adding module, a centrifugal module, a salt adding module, a ceramic proton homogenization module, a nitrogen blowing concentration module, a vortex uniform mixing module and anoscillation module, and can automatically complete sample transferring, addition of extraction liquid, extraction salt and ceramic homogenization protons, oscillation uniform mixing, centrifugal separation and vortex uniform mixing actions; A method adopting the full-automatic QuECHERS experimental device to carry out experiments mainly integrates the processes of sample liquid adding extraction,centrifugal separation, solid phase extraction and nitrogen blowing concentration, so that the time is saved, the manual operation is reduced, the automation is improved, and the errors are reduced.

The invention relates to the field of separation and purification of blood products, in particular to a method for separating and purifying blood coagulation factor VIII. According to the method, a chromatographic raw material containing the blood coagulation factor VIII is separated by using super-macroporous ion exchange chromatography. The method also comprises a step of performing coarse separation treatment before separation of the super-macroporous ion exchange chromatography. According to the method, F VIII chromatography recovery rate stably reaches up to about 85 percent, the specific activity reaches up to 154 IU/mg protein, and the process is stable and easy to amplify; while in the prior art, the F VIII chromatography recovery rate is normally stabilized at about 25 percent, and the specific activity is 20-50 IU/mg protein; and thus, compared with the prior art, the method provided by the invention has the advantage of achieving an unexpected technical effect.

The invention relates to a separation and purification method for a recombinant hepatitis B core antigen. The method includes the steps of thermal denaturation and clarification; ammonium sulfate precipitation; ultrafiltration and concentration, washing filtering and liquid exchange; depolymerization; first-step molecular sieve chromatography; ultrafiltration and concentration, washing filtering and liquid exchange; repolymerization; second-step chromatography. By means of the method, the high-purity, low-host-residue and high-stability recombinant hepatitis B core antigen with a uniform granular structure can be obtained, that is to say, the method has the advantages that the antigen purity is high, the residue of host protein and host nucleic acid is low, antigen particles are uniform, and industrial enlargement is easy.

The invention relates to the technical field of biological separation and extraction, in particular to an extracting method of extracellular vesicles. The method comprises the steps that a biologicalsample is provided; the biological sample is in contact with a capturing surface at least twice on the condition that impurities in the biological sample are partially or wholly reserved on the capturing surface, wherein the impurities are partial or whole other substances except the extracellular vesicles in the biological sample, and the capturing surface comprises an inner surface and/or outersurface of a spherical-particle porous material; when the impurities are partially or wholly reserved on the surface of a capture, breakthrough liquid is extracted liquid containing the extracellularvesicles; or the breakthrough liquid is further concentrated. By means of the method, the epicyte vesicles can be quickly separated and purified, the separation operation is simple, the single use cost is low, the separated sample in high in purity, the bioactivity of the sample is kept good, and the extracting method is applied to fundamental research in the aspects of biomarker discovery, biotherapy and the like and industrial production.

The present invention belongs to the technical field of medicine, and relates to a high-speed countercurrent chromatography continuous circulation method for separating and preparing naphthoquinone compounds from Arnebia euchroma (Royle) Johnst. According to the method, the natural naphthoquinone compounds represented by a formula (I) are separated and prepared by using a high-speed countercurrent chromatography continuous circulation separation method, n-hexane, ethyl acetate, acetonitrile and water are used as a two-phase solvent system for high-speed countercurrent chromatography separation, an online storage technology and a circulation elution mode are combined, the fractions containing the target compound are subjected to continuous circulation elution, and the fractions are collected to obtain shikonin with a purity of more than 95%, propionylshikonin with a purity of more than 95%, deoxyshikonin with a purity of more than 95%, (beta,beta-dimethylacryl)shikonin with a purity of more than 95% and isovalerylshikonin with a purity of more than 95%. With the method of the present invention, the high purity naphthoquinone compounds can be obtained through the one separation purification, such that the method has advantages of simple operation, rapidness and high efficiency, and is suitable for the separation and preparation of naphthoquinone and a variety of natural compounds. The formula (I) is defined in the specification.

The invention provides an application of a protein A chromatography medium. The protein A chromatography medium is obtained by surface-hydrophilic dendrimer modification, activation and protein A coupling by taking a polymer microsphere containing a vinyl monomer as a raw material. When being used as an antibody and Fc fusion protein separation and purification medium, the protein A chromatography medium provided by the invention is capable of greatly increasing the flow rate, short in separation time, good in separation effect and low in cost so as to be suitable for rapid separation and purification of antibodies and Fc fusion proteins.

The invention relates the segregator, comprising permanent magnet components, cover (3), separating cap (4) and sample cap (5). In the separating cap (4) there is inner edge (6) which is connected with inner wall of separating cap (4); under interior extent (6) there is rubber ring (7), and between them there is filter paper; permanent magnet components comprises N magnet and S magnet; separating cap (4) is connected with sample cap (5). The invention solves the problem that the magnetic bacteria is not easy to separate, and the invention has the advantages of simple technology, low cost, easy usage and strong practicality.

The invention relates to a method for preparing walnut green husk polyphenol from fresh walnut green husks and belongs to the technical field of walnut green husk polyphenol preparation. The technological process of the method includes the steps that fresh walnut green husks are juiced, centrifuged and subjected to solid and liquid separation, after the pH value of liquid is adjusted to be 3-4, the liquid is added into a chromatographic column in which AB-8 type resin is contained with the polyphenol concentration of the sample adding liquid being 2-3 mg/mL, pH of the sample adding liquid being 3-4, the volume of added samples being 1.6 BV and the flowing speed of the added samples being 2 BV/h, washing is performed with water, after liquid flowing out of the lower portion of the chromatographic column is colorless, elution is performed with a 65-75% ethyl alcohol, the flowing speed of elution is 1-2 BV/h, and the volume of elution is 2.10 BV. 65-75% of ethyl alcohol eluant is collected, vacuum concentration is performed, and the walnut green husk polyphenol with the content being 53% is obtained through vacuum drying. Compared with a traditional method, the steps of drying the walnut green husks and extracting the walnut green husks with an organic solvent are reduced, the contact time between the green husks and air is shortened, oxidization of the walnut green husk polyphenol is reduced, polyphenol substances are effectively protected, and the consumption of heat and the organic solvent is remarkably reduced.

The invention discloses a Method for preparation of rabdosia form element which is distilled from rabdosia, adopting 95% ethanol as extracting solvent, the warming reflux extraction time is 30-50min, then after extracting by using ethyl acetate ethyl acetate: ligroine, ligroine: petrol mixed solvent of different proportion, the extract which containing rabdosia form element will be aquired; blending by column chromatographic silica gel and loading in short range for separating and purifying. The process of this invention is easy and feasible, it is new method to separate and purify rabdosia form element and suitable for mass production, extraction. Separation, purifying and reduction in production costs can be realized fleetly, the purity of obtained rabdosia form element is above 95%.

The present invention relates to the application of antibody capable of distinguishing heat shock protein (HSP) in preparing commercial affinity resin and affinity chromatographic column, and aims at extracting HSP and heat shock protein-polypeptide complexes formed with the HSP and non-covalent combined polypeptide antigen molecules fast, effectively and specifically from protein mixing liquid.

The invention relates to a method for preparing eskaserp molecular engram polymer, belonging to biological engineering technology. It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product. The molecule engram polymer is characterized by simple, fast, high-effective separation and purification, enriching residual alserin, and direct usage for selective separation and enrichment alserin.

The invention discloses a method for separating and purifying deoxynucleoside triphosphate, which comprises the following steps: when the temperature is below 4 DEG C, depositing deoxynucleoside triphosphate biosynthesized reaction solution directly with an organic solvent to obtain a solid mixture of the deoxynucleoside triphosphate, wherein the volume ratio of the deoxynucleoside triphosphate biosynthesized reaction solution to the organic solvent is between 1:1 and 1:25; when the temperature is below the 4 DEG C, dissolving the solid mixture in ultrapure water, adjusting the pH value to between 0.1 and 2 by using acidic aqueous solution, and using the organic solvent to perform deposition again to obtain a solid matter of the deoxynucleoside triphosphate; and dissolving the solid matter in the ultrapure water, adjusting the pH value to between 5 and 11 by using sodium hydroxide alkaline aqueous solution, and performing liquid nitrogen frozen and freeze-drying to obtain a sodium salt product of the deoxynucleoside triphosphate. The method has the advantages of quick separation, good purification effect, production cost reduction, water resource pollution reduction, process energy consumption reduction, and applicability to industrial production.

The invention discloses an immobilized metal chelate chromatography medium, and a preparation method and application thereof. The preparation method comprises the following steps of: hydrolyzing macroporous polymer microspheres under an acidic condition; carrying out swelling treatment an obtained product in a functional monomer solution; stirring and mixing an obtained product and an initiator ina solvent; carrying out graft polymerization reaction at a certain pH value and a certain temperature; washing off soluble substances by using deionized water; and soaking an obtained product in a metal ion solution with a certain concentration for a certain period of time, so as to obtain the immobilized metal chelate chromatography medium. The preparation method is simple to operate and has nonon-harsh requirements for the environment; the number of functional groups on the surface of the material is effectively increased; the protein loading capacity of the medium is improved; and metal ion leakage is reduced. The prepared immobilized metal chelate chromatography medium is high in mechanical strength, low in non-specific adsorption, strong in specific binding force, high in protein binding loading capacity, high in operation flow rate and suitable for high-flux separation and purification of biomacromolecules.

The invention discloses a system for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is characterized by comprising an ultrasonic extraction device, a pre-filtering device and ceramic membrane equipment which are connected in sequence, wherein a dialyzate outlet of the ceramic membrane equipment is connected with ultrafiltration membrane equipment; a concentrated liquid outlet of the ultrafiltration membrane equipment is connected with a first vacuum concentration and spraying device; the dialyzate outlet is connected with a continuous ion exchange andspectrum separation device; the continuous ion exchange and spectrum separation device and the dialyzate outlet are sequentially connected with membrane concentration equipment and a second vacuum concentration device and spraying device. The invention further discloses a method for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is simple in process, capable of achieving separation and purification of flavones and polysaccharide from oldenlandia diffusa rapidly at a time, short in production cycle, possible in continuous material feeding and discharge, stable in operation, high in product content, full-automatic in operation, capable of saving a great amount of labor and materials and applicable to industrial popularization.

The invention provides a method for quick separation and purification of walnut protein from walnuts. The method comprises the following steps that plant raw materials containing the walnut protein are subjected to ball milling and crushed into particles, thus walnut powder is obtained, and the dry weight is weighed; petroleum ether is adopted for degreasing, and an organic solvent is volatilized;an alcohol solution is adopted as an extracting solvent, and proteolytic enzyme is added for enzymolysis; the alcohol solvent is removed through rotary evaporation, then a supercritical fluid is adopted to extract the protein in the walnuts, and extracting liquid is adsorbed through macroporous resin; and alcohol solutions with the different concentrations are adopted to elute filtrate adsorbed on the macroporous resin, thus eluant is obtained, the eluant is combined, and vacuum concentration and freezing drying are conducted to obtain a walnut protein solid.

The invention discloses a method for continuously extracting DNJ, flavonoids and polysaccharides from a moraceae plant. The method is characterized by comprising the following steps: performing ultrasonic extraction on raw materials, performing ceramic membrane filtration, performing reverse osmosis concentration and performing consecutive ion exchange for three times to respectively obtain the DNJ, the flavonoids and the polysaccharides. The invention further comprises a system for continuously extracting the DNJ, the flavonoids and the polysaccharides from the moraceae plant. The method andthe system disclosed by the invention are simple in process, capable of quickly realizing the separation and the purification of mulberry leaf and mulberry twig extracts at a time, continuous in feeding and discharging, stable in operation, high in product content and fully-automatic in system operation, saves a large quantity of manpower and material resources and is suitable for industrial popularization.