Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quickly estimating specificity of influenza A virus host

A type of influenza A virus, host-specific technology, applied in the preparation of test samples, measuring devices, material analysis by electromagnetic means, etc., can solve the problems of incomplete evaluation of influenza A virus pathogenic variation and host specificity , to achieve the effect of adapting to large-scale quarantine, high-throughput detection of viruses, and simple separation and purification

Inactive Publication Date: 2011-09-21
NORTHWEST UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problem that conventional methods for detecting avian influenza virus and detecting viral nucleic acid cannot fully evaluate the variation of influenza A virus pathogens and the specificity of the host, the present invention provides a method for rapidly evaluating the host specificity of influenza A virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly estimating specificity of influenza A virus host
  • Method for quickly estimating specificity of influenza A virus host

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] Realize the experimental material of the present invention specifically as follows:

[0064] 1), main materials and reagents:

[0065] Avian influenza virus was provided by Harbin Veterinary Research Institute; self-made hydroxylated magnetic particles;

[0066] 3'-N-Acetylneuraminyl-N-acetyllactosamine sodium salt(α-NeuNAc-(2→3)-β-D-Gal-(1→4)-D-GlcNAc) or 3'-Sialyllactose(α-NeuNAc-( 2→3)-β-D-Gal-(1→4)-D-Glc), 6'-Sialyllactose sodium salt(α-NeuNAc-(2→6)-β-D-Gal-(1→4) -D-Glc), phenylmethylsulfonphthalein fluoride (PMSF), ethanolamine, ProteinMarker, sigma; other commonly used reagents are of domestic analytical grade.

[0067] 2), the formula of main reagent:

[0068] Coupling buffer: 0.2mol / L pH5.4 acetic acid sodium acetate buffer;

[0069] Binding buffer: 20mmol / L Tris-HCl, 0.5mol / L NaCl, 10mmol / L CaCl 2 , 6mmol / L MnCl 2 , pH7.2;

[0070] Cleaning solution: 20mmol / L Tris-HCl, 0.5mol / L NaCl, 0.05% Tween20, pH7.2;

[0071] Elution buffer: 0.5% SDS;

[0072] Pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for quickly estimating specificity of an influenza A virus host. The method comprises the following steps of: primarily purifying the hemagglutinin of influenza A virus and preparing a sugar chain magnetic particle complex; combining the primarily purified hemagglutinin of the influenza A virus with the sugar chain magnetic particle complex; eluting the hemagglutinin of the influenza A virus, which is combined with the sugar chain magnetic particle complex; and identifying the eluted hemagglutinin of the influenza A virus so as to acquire the result of the specificity of the influenza A virus host and judge whether the influenza virus infects human bodies. The technical problem that the mutation of the pathogen of the influenza A virus and the specificityof the host of the influenza A virus cannot be completely estimated by the conventional influenza virus detecting means and virus nucleic acid detecting methods is solved. The method has the advantages that the method is quick, simple and convenient, has high efficiency and the like.

Description

technical field [0001] The invention relates to the field of influenza A virus, in particular to a method for evaluating host specificity of influenza A virus. Background technique [0002] Influenza A viruses are common influenza viruses and are prone to mutations. Avian influenza virus is a subtype of influenza A virus. Avian influenza is an acute infectious disease caused by avian influenza virus, which can also infect humans. Avian influenza virus not only seriously endangers the production of animal husbandry, especially the highly pathogenic H5N1 avian influenza virus has crossed the species barrier and directly caused human infection and death of the infected person. [0003] There are many types of avian influenza virus antigens and frequent mutations, making the prevention and control of highly pathogenic avian influenza very difficult. Therefore, it is necessary to develop new technologies that can be used to monitor and evaluate the variation and host specificit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N1/28G01N1/34G01N1/42G01N21/64
Inventor 李铮孙宇杜亚蓉祁会彩邓炜娜王秀荣杨刚龙
Owner NORTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com