Method for Purifying Antigen of Foot and Mouth Disease Inactivated Virus by Hydrophobic Interaction Chromatography

Abstract

The invention relates to a method for purifying a foot-and-mouth disease inactive virus antigen from a cell culture liquid. The method is characterized in that the foot-and-mouth disease inactive virus antigen is adsorbed on a chromatographic column through interaction between a hydrophobic group on the surface of a foot-and-mouth disease virus and a hydrophobic group between a hydrophobic action chromatographic packing, so the foot-and-mouth disease inactive virus antigen is separated from impurities in the liquid. The foot-and-mouth disease inactive virus antigen adsorbed on the chromatographic column can be eluted under mild eluting conditions in order to realize the purifying purpose. The method has the advantages of fast speed, few operation steps, stable process, high yield of the foot-and-mouth disease inactive virus antigen, easy amplification and industrial scale production, and large practical application values.

Description

technical field [0001] The invention relates to the field of separation and purification of vaccine antigens, in particular to a chromatographic separation method for separation and purification of foot-and-mouth disease inactivated virus vaccine antigens from cell culture fluid. Background technique [0002] Foot and Mouth Disease (FMD) is a severe infectious disease caused by Foot-and-Mouth Disease Virus (FMDV). It mainly infects cloven-hoofed animals such as cattle, sheep, and pigs. Infection, but the symptoms are relatively mild. Foot-and-mouth disease epidemics often bring huge losses to the livestock industry. Due to its strong infectivity, fast transmission speed and great harm, foot-and-mouth disease has been the focus of international health circles since the beginning of the 20th century. Countries have established research institutions to find ways to control foot-and-mouth disease. It was not until the end of the 19th century that the foot-and-mouth disease vir...

AI Technical Summary

Problems solved by technology

At present, there are two main problems with the foot-and-mouth vaccine: 1. The immune protection is unstable, and the antibody level is not detected or the antibody level is low after immunization, or the immunization period is short; 2. The safety is poor, and the side effects are strong. Food loss, food stoppage and even death after vaccination

These problems lead to long processing time, high material cost, and the antigen is also prone to lose immunological activity during the long-term processing

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